Rroxscaffold_2G00141180

Protein N-lysine methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0012035
Physical Location & Seq
Reverse (-)
79489152 .. 79489970
819 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00141180.1

Sequence Viewer

Length: 819 bp
ATGGCCGCTCTAGAAGAAGACCAAGACGACGTCGTTATAAACCCCGCCACAATGCTCCTCCGCGACGACGACTCCGACGAGGTCAAGATGGCGGAGAGCGAACAGCTCCGGCAGCATTCTCTGCCTTCGATCGAGTCCACAATCGTCACCAGGCAGCTCCCCTCGCAAGGTCTCTCCTTTCAGCTCTGGCCGGCGGCCACCACGCTCCTCACTCTCCTGGACGACCACCGCCGTGACCCCACCACCAGCCCTCTCGGCCCCACGCTCTCGGCCTTGGAATCCGGCCGGAGACTCAGGATCCTCGAACTAGGTTCTGGAACGGGCCTCGTCGGAATAGCCGCGGCCGCCACGCTCGGCGCGTGCGTCACGGTGACGGACCTCCCGCACGTCGTCGACAACCTTAAGTTCAATGCGGAACTAAACGCCGCCGTTTTGGGAGGGAGCGGCGGGAGTGTGACCGTGGCGGCGCTGAGGTGGGGAGAGGCGGAGGACGCGGAGGCGATAGGGCGGGAGTTTGATATTGTATTGGCGTCGGATGTAGTGTATCACGACCACCTCTACGAGCCGCTGCTCAAAACGCTGTCGTTTCTGCTGTTGGGGGAGGGCGGCGTGGTTTTTGTGATGGCCCACTTGAGGAGGTGGAAGAAGGAGTCGGCCTTCTTCAAGAGGGCCAGGAAGCTTTTTGAGGTTGAGGTCTTGCACGTGGATCGTCCGTGCCAGGGATCGCGGGTCGGAATTACTGTTTACCGTTTTACCGGGAAGCCAAGTAAGAAATTGTTGAAGGGTGTCTCAGTGAACATTGTCAATGGCATTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

272

Amino Acids

29.53

Weight (kDa)

5.54

Isoelectric Point (pI)

43.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_16 PF10294 54 - 225 6.4e-31 Lysine methyltransferase
Methyltransf_23 PF13489 94 - 219 5.3e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012035)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50850 AT5G49560
fragaria_vesca FvH4_1g13310
malus_domestica MD02G1147500.v1.1 MD15G1261800.v1.1
prunus_persica Prupe.7G154600_v2.0.a1
pyrus_communis pycom15g22900
rosa_chinensis RchiOBHm_Chr2g0101571
rosa_laevigata RLG00000017091
rosa_multiflora Rmu_co8339275.1_g000001 Rmu_sc0006153.1_g000007 Rmu_sc0019613.1_g000002
rosa_roxburghii Rroxscaffold_2G00141180
rosa_rugosa Rorug02G0097300
rosa_samantha Rh2AG148100 Rh2BG153500 Rh2CG154000 Rh2DG153800
rosa_wichuraiana Rw2G011550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 38
AatII GACGTC 1 cut(s) 33
AccBSI CCGCTC 2 cut(s) 8, 444
AccI GTMKAC 1 cut(s) 393
AccII CGCG 5 cut(s) 63, 341, 359, 494, 727
AclWI GGATC 4 cut(s) 292, 305, 714, 730
AcoI YGGCCR 5 cut(s) 3, 188, 195, 283, 342
AcvI CACGTG 1 cut(s) 703
AcyI GRCGYC 2 cut(s) 30, 530
AfiI CCNNNNNNNGG 3 cut(s) 167, 633, 719
AflII CTTAAG 1 cut(s) 401
AgsI TTSAA 3 cut(s) 409, 664, 781
AjiI CACGTC 1 cut(s) 388
AjnI CCWGG 4 cut(s) 149, 216, 671, 717
AleI CACNNNNGTG 1 cut(s) 231
AluBI AGCT 4 cut(s) 106, 157, 184, 679
AluI AGCT 4 cut(s) 106, 157, 184, 679
Alw26I GTCTC 3 cut(s) 176, 283, 793
AlwI GGATC 4 cut(s) 292, 305, 714, 730
ApeKI GCWGC 3 cut(s) 112, 154, 568
AspLEI GCGC 2 cut(s) 359, 469
AspS9I GGNCC 5 cut(s) 257, 322, 376, 625, 669
AsuC2I CCSGG 1 cut(s) 757
AsuHPI GGTGA 2 cut(s) 139, 382
AvaII GGWCC 1 cut(s) 376
BamHI GGATCC 1 cut(s) 297
BbrPI CACGTG 1 cut(s) 703
BbsI GAAGAC 1 cut(s) 24
BbvCI CCTCAGC 1 cut(s) 470
BbvI GCAGC 3 cut(s) 124, 166, 555
BccI CCATC 2 cut(s) 82, 616
BceAI ACGGC 2 cut(s) 216, 413
BcgI CGANNNNNNTGC 2 cut(s) 689, 723
BciT130I CCWGG 4 cut(s) 151, 218, 673, 719
BcnI CCSGG 1 cut(s) 757
BcoDI GTCTC 3 cut(s) 176, 283, 793
BfaI CTAG 2 cut(s) 11, 308
BfoI RGCGCY 1 cut(s) 470
BfrI CTTAAG 1 cut(s) 401
BglI GCCNNNNNGGC 1 cut(s) 255
Bme1390I CCNGG 5 cut(s) 151, 218, 673, 719, 757
Bme18I GGWCC 1 cut(s) 376
BmgBI CACGTC 1 cut(s) 388
BmgT120I GGNCC 5 cut(s) 257, 322, 376, 625, 669
BmiI GGNNCC 2 cut(s) 259, 299
BmrFI CCNGG 5 cut(s) 151, 218, 673, 719, 757
BpiI GAAGAC 1 cut(s) 24
Bpu10I CCTNAGC 1 cut(s) 470
BpuEI CTTGAG 1 cut(s) 652
BpuMI CCSGG 1 cut(s) 757
BsaAI YACGTR 1 cut(s) 703
BsaHI GRCGYC 2 cut(s) 30, 530
BsaI GGTCTC 1 cut(s) 176
BsaJI CCNNGG 4 cut(s) 273, 339, 459, 718
BsaXI ACNNNNNCTCC 2 cut(s) 56, 86
Bsc4I CCNNNNNNNGG 3 cut(s) 167, 633, 719
Bse118I RCCGGY 1 cut(s) 190
BseBI CCWGG 4 cut(s) 151, 218, 673, 719
BseDI CCNNGG 4 cut(s) 273, 339, 459, 718
BseGI GGATG 1 cut(s) 541
BseLI CCNNNNNNNGG 3 cut(s) 167, 633, 719
BseMII CTCAG 3 cut(s) 307, 461, 804
BseRI GAGGAG 3 cut(s) 47, 197, 649
BseX3I CGGCCG 2 cut(s) 283, 342
BseXI GCAGC 3 cut(s) 124, 166, 555
Bsh1236I CGCG 5 cut(s) 63, 341, 359, 494, 727
Bsh1285I CGRYCG 3 cut(s) 132, 286, 345
BsiEI CGRYCG 3 cut(s) 132, 286, 345
BsiSI CCGG 5 cut(s) 109, 191, 282, 286, 756
BslI CCNNNNNNNGG 3 cut(s) 167, 633, 719
BsmAI GTCTC 3 cut(s) 176, 283, 793
BsmI GAATGC 2 cut(s) 115, 810
Bso31I GGTCTC 1 cut(s) 176
Bsp143I GATC 4 cut(s) 129, 297, 706, 722
BspCNI CTCAG 3 cut(s) 306, 462, 803
BspFNI CGCG 5 cut(s) 63, 341, 359, 494, 727
BspLI GGNNCC 2 cut(s) 259, 299
BspPI GGATC 4 cut(s) 292, 305, 714, 730
BspTI CTTAAG 1 cut(s) 401
BspTNI GGTCTC 1 cut(s) 176
BsrBI CCGCTC 2 cut(s) 8, 444
BsrFI RCCGGY 1 cut(s) 190
BssAI RCCGGY 1 cut(s) 190
BssECI CCNNGG 4 cut(s) 273, 339, 459, 718
BssMI GATC 4 cut(s) 129, 297, 706, 722
BssNI GRCGYC 2 cut(s) 30, 530
BssT1I CCWWGG 1 cut(s) 273
Bst2UI CCWGG 4 cut(s) 151, 218, 673, 719
Bst4CI ACNGT 4 cut(s) 370, 460, 742, 749
BstACI GRCGYC 2 cut(s) 30, 530
BstAFI CTTAAG 1 cut(s) 401
BstAPI GCANNNNNTGC 1 cut(s) 121
BstBAI YACGTR 1 cut(s) 703
BstC8I GCNNGC 2 cut(s) 192, 361
BstDEI CTNAG 3 cut(s) 293, 470, 790
BstDSI CCRYGG 2 cut(s) 339, 459
BstF5I GGATG 1 cut(s) 541
BstFNI CGCG 5 cut(s) 63, 341, 359, 494, 727
BstH2I RGCGCY 1 cut(s) 470
BstHHI GCGC 2 cut(s) 359, 469
BstKTI GATC 4 cut(s) 132, 300, 709, 725
BstMAI GTCTC 3 cut(s) 176, 283, 793
BstMBI GATC 4 cut(s) 129, 297, 706, 722
BstMCI CGRYCG 3 cut(s) 132, 286, 345
BstMWI GCNNNNNNNGC 7 cut(s) 112, 121, 163, 255, 344, 491, 577
BstNI CCWGG 4 cut(s) 151, 218, 673, 719
BstSCI CCNGG 5 cut(s) 149, 216, 671, 717, 755
BstUI CGCG 5 cut(s) 63, 341, 359, 494, 727
BstV1I GCAGC 3 cut(s) 124, 166, 555
BstV2I GAAGAC 1 cut(s) 24
BstX2I RGATCY 1 cut(s) 297
BstYI RGATCY 1 cut(s) 297
BstZI CGGCCG 2 cut(s) 283, 342
BtgI CCRYGG 2 cut(s) 339, 459
BtrI CACGTC 1 cut(s) 388
BtsCI GGATG 1 cut(s) 541
BtsIMutI CAGTG 1 cut(s) 798
Cac8I GCNNGC 2 cut(s) 192, 361
CciNI GCGGCCGC 1 cut(s) 342
CfoI GCGC 2 cut(s) 359, 469
Cfr10I RCCGGY 1 cut(s) 190
Cfr13I GGNCC 5 cut(s) 257, 322, 376, 625, 669
Cfr42I CCGCGG 1 cut(s) 342
CseI GACGC 3 cut(s) 352, 500, 519
DdeI CTNAG 3 cut(s) 293, 470, 790
DpnI GATC 4 cut(s) 131, 299, 708, 724
DpnII GATC 4 cut(s) 129, 297, 706, 722
EaeI YGGCCR 5 cut(s) 3, 188, 195, 283, 342
EagI CGGCCG 2 cut(s) 283, 342
EciI GGCGGA 2 cut(s) 107, 500
EclXI CGGCCG 2 cut(s) 283, 342
Eco130I CCWWGG 1 cut(s) 273
Eco31I GGTCTC 1 cut(s) 176
Eco47I GGWCC 1 cut(s) 376
Eco52I CGGCCG 2 cut(s) 283, 342
Eco72I CACGTG 1 cut(s) 703
EcoRII CCWGG 4 cut(s) 149, 216, 671, 717
EcoT14I CCWWGG 1 cut(s) 273
ErhI CCWWGG 1 cut(s) 273
FaiI YATR 1 cut(s) 38
FauI CCCGC 5 cut(s) 52, 390, 440, 501, 720
FblI GTMKAC 1 cut(s) 393
FokI GGATG 1 cut(s) 548
FspBI CTAG 2 cut(s) 11, 308
GlaI GCGC 2 cut(s) 358, 468
HaeII RGCGCY 1 cut(s) 470
HapII CCGG 5 cut(s) 109, 191, 282, 286, 756
HgaI GACGC 3 cut(s) 352, 500, 519
HhaI GCGC 2 cut(s) 359, 469
Hin1I GRCGYC 2 cut(s) 30, 530
Hin6I GCGC 2 cut(s) 357, 467
HinP1I GCGC 2 cut(s) 357, 467
HincII GTYRAC 1 cut(s) 394
HindII GTYRAC 1 cut(s) 394
HindIII AAGCTT 1 cut(s) 677
HinfI GANTC 5 cut(s) 71, 134, 278, 291, 650
HpaII CCGG 5 cut(s) 109, 191, 282, 286, 756
HphI GGTGA 2 cut(s) 139, 382
Hpy166II GTNNAC 4 cut(s) 138, 394, 745, 796
Hpy188I TCNGA 4 cut(s) 76, 332, 535, 734
Hpy188III TCNNGA 6 cut(s) 11, 85, 295, 315, 548, 664
Hpy8I GTNNAC 4 cut(s) 138, 394, 745, 796
Hpy99I CGWCG 9 cut(s) 32, 35, 68, 71, 80, 332, 392, 395, 535
HpyAV CCTTC 4 cut(s) 135, 640, 667, 775
HpyCH4III ACNGT 4 cut(s) 370, 460, 742, 749
HpyCH4IV ACGT 3 cut(s) 30, 387, 702
HpyCH4V TGCA 1 cut(s) 700
HpyF10VI GCNNNNNNNGC 7 cut(s) 112, 121, 163, 255, 344, 491, 577
HpyF3I CTNAG 3 cut(s) 293, 470, 790
HpySE526I ACGT 3 cut(s) 30, 387, 702
Hsp92I GRCGYC 2 cut(s) 30, 530
HspAI GCGC 2 cut(s) 357, 467
KroI GCCGGC 1 cut(s) 190
KroNI GCCGGC 1 cut(s) 192
KspI CCGCGG 1 cut(s) 342
Kzo9I GATC 4 cut(s) 129, 297, 706, 722
LmnI GCTCC 5 cut(s) 60, 111, 162, 210, 441
Lsp1109I GCAGC 3 cut(s) 124, 166, 555
MaeI CTAG 2 cut(s) 11, 308
MaeII ACGT 3 cut(s) 30, 387, 702
MaeIII GTNAC 5 cut(s) 145, 233, 364, 370, 454
MalI GATC 4 cut(s) 131, 299, 708, 724
MbiI CCGCTC 2 cut(s) 8, 444
MboI GATC 4 cut(s) 129, 297, 706, 722
MboII GAAGA 4 cut(s) 26, 29, 652, 655
MflI RGATCY 1 cut(s) 297
MluCI AATT 2 cut(s) 735, 773
MlyI GAGTC 4 cut(s) 65, 143, 285, 659
MmeI TCCRAC 4 cut(s) 99, 310, 513, 712
MroNI GCCGGC 1 cut(s) 190
MseI TTAA 1 cut(s) 402
MslI CAYNNNNRTG 1 cut(s) 231
MspA1I CMGCKG 2 cut(s) 341, 568
MspCI CTTAAG 1 cut(s) 401
MspI CCGG 5 cut(s) 109, 191, 282, 286, 756
MspR9I CCNGG 5 cut(s) 151, 218, 673, 719, 757
Mva1269I GAATGC 2 cut(s) 115, 810
MvaI CCWGG 4 cut(s) 151, 218, 673, 719
MvnI CGCG 5 cut(s) 63, 341, 359, 494, 727
MwoI GCNNNNNNNGC 7 cut(s) 112, 121, 163, 255, 344, 491, 577
NaeI GCCGGC 1 cut(s) 192
NciI CCSGG 1 cut(s) 757
NdeII GATC 4 cut(s) 129, 297, 706, 722
NgoMIV GCCGGC 1 cut(s) 190
NlaIV GGNNCC 2 cut(s) 259, 299
NmeAIII GCCGAG 3 cut(s) 234, 248, 333
NmuCI GTSAC 5 cut(s) 145, 233, 364, 370, 454
NotI GCGGCCGC 1 cut(s) 342
OliI CACNNNNGTG 1 cut(s) 231
PcsI WCGNNNNNNNCGW 4 cut(s) 72, 75, 356, 360
PctI GAATGC 2 cut(s) 115, 810
PdiI GCCGGC 1 cut(s) 192
PfeI GAWTC 1 cut(s) 278
PflFI GACNNNGTC 2 cut(s) 29, 80
PfoI TCCNGGA 1 cut(s) 216
Ple19I CGATCG 1 cut(s) 132
PleI GAGTC 4 cut(s) 65, 142, 285, 658
PmaCI CACGTG 1 cut(s) 703
PmlI CACGTG 1 cut(s) 703
PpsI GAGTC 4 cut(s) 65, 142, 285, 658
Ppu21I YACGTR 1 cut(s) 703
PsiI TTATAA 1 cut(s) 38
Psp6I CCWGG 4 cut(s) 149, 216, 671, 717
PspCI CACGTG 1 cut(s) 703
PspGI CCWGG 4 cut(s) 149, 216, 671, 717
PspN4I GGNNCC 2 cut(s) 259, 299
PspPI GGNCC 5 cut(s) 257, 322, 376, 625, 669
PsuI RGATCY 1 cut(s) 297
PsyI GACNNNGTC 2 cut(s) 29, 80
PvuI CGATCG 1 cut(s) 132
RseI CAYNNNNRTG 1 cut(s) 231
SacII CCGCGG 1 cut(s) 342
SalI GTCGAC 1 cut(s) 392
SaqAI TTAA 1 cut(s) 402
Sau3AI GATC 4 cut(s) 129, 297, 706, 722
Sau96I GGNCC 5 cut(s) 257, 322, 376, 625, 669
SchI GAGTC 4 cut(s) 65, 143, 285, 659
ScrFI CCNGG 5 cut(s) 151, 218, 673, 719, 757
Sfr303I CCGCGG 1 cut(s) 342
SgrBI CCGCGG 1 cut(s) 342
SinI GGWCC 1 cut(s) 376
SmiMI CAYNNNNRTG 1 cut(s) 231
SmlI CTYRAG 2 cut(s) 401, 631
SmoI CTYRAG 2 cut(s) 401, 631
Sse9I AATT 2 cut(s) 735, 773
SspMI CTAG 2 cut(s) 11, 308
StyD4I CCNGG 5 cut(s) 149, 216, 671, 717, 755
StyI CCWWGG 1 cut(s) 273
TaaI ACNGT 4 cut(s) 370, 460, 742, 749
TaiI ACGT 3 cut(s) 33, 390, 705
TaqI TCGA 4 cut(s) 128, 132, 303, 393
TasI AATT 2 cut(s) 735, 773
TfiI GAWTC 1 cut(s) 278
Tru1I TTAA 1 cut(s) 402
Tru9I TTAA 1 cut(s) 402
TscAI CASTG 1 cut(s) 798
TseFI GTSAC 5 cut(s) 145, 233, 364, 370, 454
TseI GCWGC 3 cut(s) 112, 154, 568
Tsp45I GTSAC 5 cut(s) 145, 233, 364, 370, 454
TspGWI ACGGA 2 cut(s) 389, 702
TspRI CASTG 1 cut(s) 798
Tth111I GACNNNGTC 2 cut(s) 29, 80
Vha464I CTTAAG 1 cut(s) 401
VpaK11BI GGWCC 1 cut(s) 376
XbaI TCTAGA 1 cut(s) 10
XmiI GTMKAC 1 cut(s) 393
XspI CTAG 2 cut(s) 11, 308
ZraI GACGTC 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.