Rw2G011550

Protein N-lysine methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0012035
Physical Location & Seq
Forward (+)
11662482 .. 11663295
814 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G011550.1

Sequence Viewer

Length: 777 bp
ATGGCCGCTCTAGAAGAAGACCAAGACGACGTCGTTATAAACCCCGCCACAATGCTCCTCCGCCTCCGGCAGCATTCTCTGCCTTCGATCGACTCCACAATCGTCACCAGGCAGCTCCCCTCGCAGGGTCTCTCCTTTCAGCTCTGGCCGGCGGCCACCACGCTCCTCACTCTCCTGGACGACCACCGCCGTGACCCCACCACGAGCCCCCTTGGCCCCACGCTCTCGGCCTTGGAATCCGGCCGGAGACTCAAGATCCTCGAACTAGGTTCTGGAACCGGCCTCGTCGGAATAGCCGCCGCTGCCACGCTCGGCGCGTGCGTCACTGTGACGGACCTCCCGCACGTCGTCGACAACCTTAAGTTCAATGCGGAACTAAACGCCGCCGCTTTGGGAGGGAGCGGCGGGAGTGTGAGAGTGGCGGCGCTGAGGTGGGGAGAGGCGGAGGACGCGGAGGCAATAGGGCGGGAGTTTGATATTGTATTGGCGTCGGATGTAGTGTATCACGACCACCTCTACGAGCCGCTGCTCAAAACGCTGTCGTTTCTGCTGTTGGGGGAGGGCGGCGTGGTGTTTGTGATGGCCCACTTGAGGAGGTGGAAGAAGGAGTCGGCCTTCTTCAAGAGGGCCAGGAAGCTTTTTGAGGTTGAGGTCTTGCACGTGGATCGTCCGTGCCAGGGATCGAGGGTCGGAGTTACTGTTTACCGTTTTACCGGGAAGCCAAGTAAGAAATTGTTGAATGGTGTCTCTGTGAACATTGTCAATGGCATTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

258

Amino Acids

27.91

Weight (kDa)

6.66

Isoelectric Point (pI)

37.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_16 PF10294 40 - 211 4.1e-31 Lysine methyltransferase
Methyltransf_23 PF13489 80 - 205 6.6e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012035)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50850 AT5G49560
fragaria_vesca FvH4_1g13310
malus_domestica MD02G1147500.v1.1 MD15G1261800.v1.1
prunus_persica Prupe.7G154600_v2.0.a1
pyrus_communis pycom15g22900
rosa_chinensis RchiOBHm_Chr2g0101571
rosa_laevigata RLG00000017091
rosa_multiflora Rmu_co8339275.1_g000001 Rmu_sc0006153.1_g000007 Rmu_sc0019613.1_g000002
rosa_roxburghii Rroxscaffold_2G00141180
rosa_rugosa Rorug02G0097300
rosa_samantha Rh2AG148100 Rh2BG153500 Rh2CG154000 Rh2DG153800
rosa_wichuraiana Rw2G011550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 38
AatII GACGTC 1 cut(s) 33
AccBSI CCGCTC 2 cut(s) 8, 402
AccI GTMKAC 1 cut(s) 351
AccII CGCG 2 cut(s) 317, 452
AclWI GGATC 3 cut(s) 250, 672, 688
AcoI YGGCCR 4 cut(s) 3, 146, 153, 241
AcvI CACGTG 1 cut(s) 661
AcyI GRCGYC 2 cut(s) 30, 488
AfiI CCNNNNNNNGG 4 cut(s) 124, 125, 591, 677
AflII CTTAAG 1 cut(s) 359
AgsI TTSAA 3 cut(s) 367, 622, 739
AjiI CACGTC 1 cut(s) 346
AjnI CCWGG 4 cut(s) 107, 174, 629, 675
AleI CACNNNNGTG 1 cut(s) 189
AluBI AGCT 3 cut(s) 115, 142, 637
AluI AGCT 3 cut(s) 115, 142, 637
Alw26I GTCTC 3 cut(s) 134, 241, 751
AlwI GGATC 3 cut(s) 250, 672, 688
ApeKI GCWGC 4 cut(s) 70, 112, 302, 526
AspLEI GCGC 2 cut(s) 317, 427
AspS9I GGNCC 4 cut(s) 215, 334, 583, 627
AsuC2I CCSGG 1 cut(s) 715
AsuHPI GGTGA 1 cut(s) 97
AvaII GGWCC 1 cut(s) 334
BanII GRGCYC 1 cut(s) 209
BauI CACGAG 1 cut(s) 202
BbrPI CACGTG 1 cut(s) 661
BbsI GAAGAC 1 cut(s) 24
BbvCI CCTCAGC 1 cut(s) 428
BbvI GCAGC 4 cut(s) 82, 124, 289, 513
BccI CCATC 1 cut(s) 574
BceAI ACGGC 1 cut(s) 174
BcgI CGANNNNNNTGC 2 cut(s) 647, 681
BciT130I CCWGG 4 cut(s) 109, 176, 631, 677
BcnI CCSGG 1 cut(s) 715
BcoDI GTCTC 3 cut(s) 134, 241, 751
BfaI CTAG 2 cut(s) 11, 266
BfoI RGCGCY 1 cut(s) 428
BfrI CTTAAG 1 cut(s) 359
BglI GCCNNNNNGGC 1 cut(s) 213
Bme1390I CCNGG 5 cut(s) 109, 176, 631, 677, 715
Bme18I GGWCC 1 cut(s) 334
BmgBI CACGTC 1 cut(s) 346
BmgT120I GGNCC 4 cut(s) 215, 334, 583, 627
BmiI GGNNCC 2 cut(s) 217, 277
BmrFI CCNGG 5 cut(s) 109, 176, 631, 677, 715
BpiI GAAGAC 1 cut(s) 24
Bpu10I CCTNAGC 1 cut(s) 428
BpuEI CTTGAG 2 cut(s) 236, 610
BpuMI CCSGG 1 cut(s) 715
BsaAI YACGTR 1 cut(s) 661
BsaHI GRCGYC 2 cut(s) 30, 488
BsaI GGTCTC 1 cut(s) 134
BsaJI CCNNGG 3 cut(s) 211, 231, 676
BsaXI ACNNNNNCTCC 2 cut(s) 684, 714
Bsc4I CCNNNNNNNGG 4 cut(s) 124, 125, 591, 677
Bse118I RCCGGY 2 cut(s) 148, 278
BseBI CCWGG 4 cut(s) 109, 176, 631, 677
BseDI CCNNGG 3 cut(s) 211, 231, 676
BseGI GGATG 1 cut(s) 499
BseLI CCNNNNNNNGG 4 cut(s) 124, 125, 591, 677
BseMII CTCAG 1 cut(s) 419
BseRI GAGGAG 3 cut(s) 47, 155, 607
BseX3I CGGCCG 1 cut(s) 241
BseXI GCAGC 4 cut(s) 82, 124, 289, 513
Bsh1236I CGCG 2 cut(s) 317, 452
Bsh1285I CGRYCG 2 cut(s) 90, 244
BsiEI CGRYCG 2 cut(s) 90, 244
BsiSI CCGG 6 cut(s) 67, 149, 240, 244, 279, 714
BslI CCNNNNNNNGG 4 cut(s) 124, 125, 591, 677
BsmAI GTCTC 3 cut(s) 134, 241, 751
BsmI GAATGC 2 cut(s) 73, 768
Bso31I GGTCTC 1 cut(s) 134
Bsp1286I GDGCHC 1 cut(s) 209
Bsp143I GATC 4 cut(s) 87, 255, 664, 680
BspCNI CTCAG 1 cut(s) 420
BspFNI CGCG 2 cut(s) 317, 452
BspLI GGNNCC 2 cut(s) 217, 277
BspPI GGATC 3 cut(s) 250, 672, 688
BspTI CTTAAG 1 cut(s) 359
BspTNI GGTCTC 1 cut(s) 134
BsrBI CCGCTC 2 cut(s) 8, 402
BsrFI RCCGGY 2 cut(s) 148, 278
BssAI RCCGGY 2 cut(s) 148, 278
BssECI CCNNGG 3 cut(s) 211, 231, 676
BssMI GATC 4 cut(s) 87, 255, 664, 680
BssNI GRCGYC 2 cut(s) 30, 488
BssSI CACGAG 1 cut(s) 202
BssT1I CCWWGG 2 cut(s) 211, 231
Bst2BI CACGAG 1 cut(s) 202
Bst2UI CCWGG 4 cut(s) 109, 176, 631, 677
Bst4CI ACNGT 3 cut(s) 328, 700, 707
BstACI GRCGYC 2 cut(s) 30, 488
BstAFI CTTAAG 1 cut(s) 359
BstAPI GCANNNNNTGC 1 cut(s) 79
BstBAI YACGTR 1 cut(s) 661
BstC8I GCNNGC 2 cut(s) 150, 319
BstDEI CTNAG 1 cut(s) 428
BstF5I GGATG 1 cut(s) 499
BstFNI CGCG 2 cut(s) 317, 452
BstH2I RGCGCY 1 cut(s) 428
BstHHI GCGC 2 cut(s) 317, 427
BstKTI GATC 4 cut(s) 90, 258, 667, 683
BstMAI GTCTC 3 cut(s) 134, 241, 751
BstMBI GATC 4 cut(s) 87, 255, 664, 680
BstMCI CGRYCG 2 cut(s) 90, 244
BstMWI GCNNNNNNNGC 6 cut(s) 79, 121, 213, 302, 449, 535
BstNI CCWGG 4 cut(s) 109, 176, 631, 677
BstSCI CCNGG 5 cut(s) 107, 174, 629, 675, 713
BstUI CGCG 2 cut(s) 317, 452
BstV1I GCAGC 4 cut(s) 82, 124, 289, 513
BstV2I GAAGAC 1 cut(s) 24
BstX2I RGATCY 1 cut(s) 255
BstYI RGATCY 1 cut(s) 255
BstZI CGGCCG 1 cut(s) 241
BtrI CACGTC 1 cut(s) 346
BtsCI GGATG 1 cut(s) 499
BtsIMutI CAGTG 1 cut(s) 324
Cac8I GCNNGC 2 cut(s) 150, 319
CfoI GCGC 2 cut(s) 317, 427
Cfr10I RCCGGY 2 cut(s) 148, 278
Cfr13I GGNCC 4 cut(s) 215, 334, 583, 627
CseI GACGC 3 cut(s) 310, 458, 477
DdeI CTNAG 1 cut(s) 428
DpnI GATC 4 cut(s) 89, 257, 666, 682
DpnII GATC 4 cut(s) 87, 255, 664, 680
EaeI YGGCCR 4 cut(s) 3, 146, 153, 241
EagI CGGCCG 1 cut(s) 241
EciI GGCGGA 2 cut(s) 50, 458
EclXI CGGCCG 1 cut(s) 241
Eco130I CCWWGG 2 cut(s) 211, 231
Eco24I GRGCYC 1 cut(s) 209
Eco31I GGTCTC 1 cut(s) 134
Eco47I GGWCC 1 cut(s) 334
Eco52I CGGCCG 1 cut(s) 241
Eco72I CACGTG 1 cut(s) 661
EcoRII CCWGG 4 cut(s) 107, 174, 629, 675
EcoT14I CCWWGG 2 cut(s) 211, 231
EcoT38I GRGCYC 1 cut(s) 209
ErhI CCWWGG 2 cut(s) 211, 231
FaiI YATR 1 cut(s) 38
FauI CCCGC 4 cut(s) 52, 348, 398, 459
FblI GTMKAC 1 cut(s) 351
FokI GGATG 1 cut(s) 506
FriOI GRGCYC 1 cut(s) 209
FspBI CTAG 2 cut(s) 11, 266
GlaI GCGC 2 cut(s) 316, 426
HaeII RGCGCY 1 cut(s) 428
HapII CCGG 6 cut(s) 67, 149, 240, 244, 279, 714
HgaI GACGC 3 cut(s) 310, 458, 477
HhaI GCGC 2 cut(s) 317, 427
Hin1I GRCGYC 2 cut(s) 30, 488
Hin6I GCGC 2 cut(s) 315, 425
HinP1I GCGC 2 cut(s) 315, 425
HincII GTYRAC 1 cut(s) 352
HindII GTYRAC 1 cut(s) 352
HindIII AAGCTT 1 cut(s) 635
HinfI GANTC 4 cut(s) 92, 236, 249, 608
HpaII CCGG 6 cut(s) 67, 149, 240, 244, 279, 714
HphI GGTGA 1 cut(s) 97
Hpy166II GTNNAC 3 cut(s) 352, 703, 754
Hpy188I TCNGA 3 cut(s) 290, 493, 692
Hpy188III TCNNGA 5 cut(s) 11, 253, 273, 506, 622
Hpy8I GTNNAC 3 cut(s) 352, 703, 754
Hpy99I CGWCG 6 cut(s) 32, 35, 290, 350, 353, 493
HpyAV CCTTC 3 cut(s) 93, 598, 625
HpyCH4III ACNGT 3 cut(s) 328, 700, 707
HpyCH4IV ACGT 3 cut(s) 30, 345, 660
HpyCH4V TGCA 1 cut(s) 658
HpyF10VI GCNNNNNNNGC 6 cut(s) 79, 121, 213, 302, 449, 535
HpyF3I CTNAG 1 cut(s) 428
HpySE526I ACGT 3 cut(s) 30, 345, 660
Hsp92I GRCGYC 2 cut(s) 30, 488
HspAI GCGC 2 cut(s) 315, 425
KroI GCCGGC 1 cut(s) 148
KroNI GCCGGC 1 cut(s) 150
Kzo9I GATC 4 cut(s) 87, 255, 664, 680
LmnI GCTCC 4 cut(s) 60, 120, 168, 399
Lsp1109I GCAGC 4 cut(s) 82, 124, 289, 513
MaeI CTAG 2 cut(s) 11, 266
MaeII ACGT 3 cut(s) 30, 345, 660
MaeIII GTNAC 5 cut(s) 103, 191, 322, 328, 694
MalI GATC 4 cut(s) 89, 257, 666, 682
MbiI CCGCTC 2 cut(s) 8, 402
MboI GATC 4 cut(s) 87, 255, 664, 680
MboII GAAGA 4 cut(s) 26, 29, 610, 613
MflI RGATCY 1 cut(s) 255
MhlI GDGCHC 1 cut(s) 209
MluCI AATT 1 cut(s) 731
MlyI GAGTC 3 cut(s) 86, 243, 617
MmeI TCCRAC 3 cut(s) 268, 471, 670
MroNI GCCGGC 1 cut(s) 148
MseI TTAA 1 cut(s) 360
MslI CAYNNNNRTG 1 cut(s) 189
MspA1I CMGCKG 2 cut(s) 302, 526
MspCI CTTAAG 1 cut(s) 359
MspI CCGG 6 cut(s) 67, 149, 240, 244, 279, 714
MspR9I CCNGG 5 cut(s) 109, 176, 631, 677, 715
Mva1269I GAATGC 2 cut(s) 73, 768
MvaI CCWGG 4 cut(s) 109, 176, 631, 677
MvnI CGCG 2 cut(s) 317, 452
MwoI GCNNNNNNNGC 6 cut(s) 79, 121, 213, 302, 449, 535
NaeI GCCGGC 1 cut(s) 150
NciI CCSGG 1 cut(s) 715
NdeII GATC 4 cut(s) 87, 255, 664, 680
NgoMIV GCCGGC 1 cut(s) 148
NlaIV GGNNCC 2 cut(s) 217, 277
NmeAIII GCCGAG 2 cut(s) 206, 291
NmuCI GTSAC 4 cut(s) 103, 191, 322, 328
OliI CACNNNNGTG 1 cut(s) 189
PcsI WCGNNNNNNNCGW 2 cut(s) 314, 318
PctI GAATGC 2 cut(s) 73, 768
PdiI GCCGGC 1 cut(s) 150
PfeI GAWTC 1 cut(s) 236
PflFI GACNNNGTC 1 cut(s) 29
PfoI TCCNGGA 1 cut(s) 174
Ple19I CGATCG 1 cut(s) 90
PleI GAGTC 3 cut(s) 86, 243, 616
PmaCI CACGTG 1 cut(s) 661
PmlI CACGTG 1 cut(s) 661
PpsI GAGTC 3 cut(s) 86, 243, 616
Ppu21I YACGTR 1 cut(s) 661
PsiI TTATAA 1 cut(s) 38
Psp6I CCWGG 4 cut(s) 107, 174, 629, 675
PspCI CACGTG 1 cut(s) 661
PspGI CCWGG 4 cut(s) 107, 174, 629, 675
PspN4I GGNNCC 2 cut(s) 217, 277
PspPI GGNCC 4 cut(s) 215, 334, 583, 627
PsuI RGATCY 1 cut(s) 255
PsyI GACNNNGTC 1 cut(s) 29
PvuI CGATCG 1 cut(s) 90
RseI CAYNNNNRTG 1 cut(s) 189
SalI GTCGAC 1 cut(s) 350
SaqAI TTAA 1 cut(s) 360
Sau3AI GATC 4 cut(s) 87, 255, 664, 680
Sau96I GGNCC 4 cut(s) 215, 334, 583, 627
SchI GAGTC 3 cut(s) 86, 243, 617
ScrFI CCNGG 5 cut(s) 109, 176, 631, 677, 715
SduI GDGCHC 1 cut(s) 209
SinI GGWCC 1 cut(s) 334
SmiMI CAYNNNNRTG 1 cut(s) 189
SmlI CTYRAG 3 cut(s) 251, 359, 589
SmoI CTYRAG 3 cut(s) 251, 359, 589
Sse9I AATT 1 cut(s) 731
SspMI CTAG 2 cut(s) 11, 266
StyD4I CCNGG 5 cut(s) 107, 174, 629, 675, 713
StyI CCWWGG 2 cut(s) 211, 231
TaaI ACNGT 3 cut(s) 328, 700, 707
TaiI ACGT 3 cut(s) 33, 348, 663
TaqI TCGA 5 cut(s) 86, 90, 261, 351, 683
TasI AATT 1 cut(s) 731
TfiI GAWTC 1 cut(s) 236
Tru1I TTAA 1 cut(s) 360
Tru9I TTAA 1 cut(s) 360
TscAI CASTG 1 cut(s) 331
TseFI GTSAC 4 cut(s) 103, 191, 322, 328
TseI GCWGC 4 cut(s) 70, 112, 302, 526
Tsp45I GTSAC 4 cut(s) 103, 191, 322, 328
TspGWI ACGGA 2 cut(s) 347, 660
TspRI CASTG 1 cut(s) 331
Tth111I GACNNNGTC 1 cut(s) 29
Vha464I CTTAAG 1 cut(s) 359
VpaK11BI GGWCC 1 cut(s) 334
XbaI TCTAGA 1 cut(s) 10
XmiI GTMKAC 1 cut(s) 351
XspI CTAG 2 cut(s) 11, 266
ZraI GACGTC 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.