Prupe.7G154600_v2.0.a1

Protein N-lysine methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0012035
Physical Location & Seq
Reverse (-)
16416593 .. 16417396
804 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G154600.1

Sequence Viewer

Length: 804 bp
ATGGCCGCTCAACAAGGCGACGGTTCTGAAGACGATGCAACTCTAAGCCCTGTCACCGCCGCCATTCTCCTTCCACCGACTGAACATTGCCTCCACTCCATCAACTCAACAGTCTTCATCCGGCAGCTTCCGTCTCAAGGTCTCTCCTTCCAGCTCTGGCCAGCCGCCACCACGTTCCTCACTCTCCTCGACGACCACCGCCGTAACCCTAGCTCCGGCCCGCTGGGCCCCACTCTCACGGCCTTGGGCTCGCGTCGCCCCCCGCTCAAAATCCTCGAGCTCGGCTCCGGAACCGGCCTCGTCGGAATCGCAGCCGCTGTCACGCTCGGAGCTAGCGTCACGGTCACCGACCTCCCGCACGTCATTCCTAACCTTTCGTTTAACGTAAAAGCGAATGCCGCCGTTTTGGCCGCGAACGGCGGGGTCGTCCACGTGGCGGCGCTGAGATGGGGAGAGGCCGAGGACGTGGAGGTGATTGGGCGGGAGTTTGATCTTATCGTAGCCTCGGATGTAGTGTACCACGATCACCTGTACGAACCCTTGCTCAAAACGCTGCGTTTGTTGATAATGGGTGGGGAGGAGGCGGAGGAGGGAAGGGTGTTTGTGATGGCCCACTTGAGGAGGTGGAAGAAGGACTCGGCTTTCTTCAAGAAGGCAAGGAAGCTTTTTGAGGTTGAGGTTTTGCACGTGGACCCACCGTGCCATGGCTCCAGGGTTGGAGTTACTGTTTACCGTTTTGCAGGGAAAAAGTCAAGTAAGAAATTGTTGAAAGCAAACAATGCCAATGCCATTGCCATTGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

268

Amino Acids

28.64

Weight (kDa)

7.87

Isoelectric Point (pI)

40.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012035)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50850 AT5G49560
fragaria_vesca FvH4_1g13310
malus_domestica MD02G1147500.v1.1 MD15G1261800.v1.1
prunus_persica Prupe.7G154600_v2.0.a1
pyrus_communis pycom15g22900
rosa_chinensis RchiOBHm_Chr2g0101571
rosa_laevigata RLG00000017091
rosa_multiflora Rmu_co8339275.1_g000001 Rmu_sc0006153.1_g000007 Rmu_sc0019613.1_g000002
rosa_roxburghii Rroxscaffold_2G00141180
rosa_rugosa Rorug02G0097300
rosa_samantha Rh2AG148100 Rh2BG153500 Rh2CG154000 Rh2DG153800
rosa_wichuraiana Rw2G011550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 8, 265
AccII CGCG 2 cut(s) 253, 413
AccIII TCCGGA 1 cut(s) 287
AcoI YGGCCR 3 cut(s) 3, 158, 408
AcuI CTGAAG 1 cut(s) 48
AcvI CACGTG 2 cut(s) 433, 688
AfaI GTAC 2 cut(s) 518, 533
AfiI CCNNNNNNNGG 5 cut(s) 137, 215, 436, 618, 704
AgsI TTSAA 2 cut(s) 649, 769
AjiI CACGTC 2 cut(s) 361, 466
AjnI CCWGG 1 cut(s) 710
AloI GAACNNNNNNTCC 2 cut(s) 75, 107
AluBI AGCT 6 cut(s) 127, 154, 213, 280, 332, 664
AluI AGCT 6 cut(s) 127, 154, 213, 280, 332, 664
Alw21I GWGCWC 1 cut(s) 282
Alw26I GTCTC 2 cut(s) 138, 146
AlwNI CAGNNNCTG 1 cut(s) 317
Ama87I CYCGRG 1 cut(s) 275
Aor13HI TCCGGA 1 cut(s) 287
AoxI GGCC 9 cut(s) 3, 158, 217, 226, 240, 295, 408, 456, 609
ApaI GGGCCC 1 cut(s) 230
ApeKI GCWGC 3 cut(s) 124, 311, 553
AspLEI GCGC 1 cut(s) 442
AspS9I GGNCC 5 cut(s) 218, 226, 227, 610, 691
AsuHPI GGTGA 4 cut(s) 46, 337, 484, 518
AsuNHI GCTAGC 1 cut(s) 332
AvaI CYCGRG 1 cut(s) 275
AvaII GGWCC 1 cut(s) 691
BaeGI GKGCMC 1 cut(s) 230
BalI TGGCCA 1 cut(s) 160
BanII GRGCYC 3 cut(s) 230, 251, 282
BbrPI CACGTG 2 cut(s) 433, 688
BbsI GAAGAC 2 cut(s) 36, 106
Bbv12I GWGCWC 1 cut(s) 282
BbvI GCAGC 3 cut(s) 136, 323, 540
BccI CCATC 3 cut(s) 107, 441, 601
BceAI ACGGC 4 cut(s) 186, 255, 386, 433
BciT130I CCWGG 1 cut(s) 712
BcoDI GTCTC 2 cut(s) 138, 146
BfaI CTAG 2 cut(s) 210, 333
BfoI RGCGCY 1 cut(s) 443
BglI GCCNNNNNGGC 2 cut(s) 225, 407
Bme1390I CCNGG 1 cut(s) 712
Bme18I GGWCC 1 cut(s) 691
BmeT110I CYCGRG 1 cut(s) 275
BmgBI CACGTC 2 cut(s) 361, 466
BmgT120I GGNCC 5 cut(s) 218, 226, 227, 610, 691
BmiI GGNNCC 6 cut(s) 228, 229, 286, 292, 693, 709
BmrFI CCNGG 1 cut(s) 712
BmsI GCATC 1 cut(s) 25
BmtI GCTAGC 1 cut(s) 336
BpiI GAAGAC 2 cut(s) 36, 106
BplI GAGNNNNNCTC 2 cut(s) 269, 301
BpmI CTGGAG 1 cut(s) 694
BpuEI CTTGAG 2 cut(s) 120, 637
BsaAI YACGTR 2 cut(s) 433, 688
BsaI GGTCTC 1 cut(s) 146
BsaJI CCNNGG 5 cut(s) 243, 459, 504, 703, 711
BsaWI WCCGGW 1 cut(s) 287
BsaXI ACNNNNNCTCC 8 cut(s) 75, 105, 197, 227, 321, 351, 711, 741
Bsc4I CCNNNNNNNGG 5 cut(s) 137, 215, 436, 618, 704
Bse118I RCCGGY 1 cut(s) 293
Bse3DI GCAATG 3 cut(s) 85, 789, 795
BseAI TCCGGA 1 cut(s) 287
BseBI CCWGG 1 cut(s) 712
BseDI CCNNGG 5 cut(s) 243, 459, 504, 703, 711
BseGI GGATG 2 cut(s) 117, 514
BseLI CCNNNNNNNGG 5 cut(s) 137, 215, 436, 618, 704
BseMI GCAATG 3 cut(s) 85, 789, 795
BseMII CTCAG 1 cut(s) 434
BseRI GAGGAG 4 cut(s) 176, 593, 602, 634
BseSI GKGCMC 1 cut(s) 230
BseXI GCAGC 3 cut(s) 136, 323, 540
BseYI CCCAGC 1 cut(s) 223
Bsh1236I CGCG 2 cut(s) 253, 413
BshFI GGCC 9 cut(s) 5, 160, 219, 228, 242, 297, 410, 458, 611
BsiHKAI GWGCWC 1 cut(s) 282
BsiHKCI CYCGRG 1 cut(s) 275
BsiSI CCGG 4 cut(s) 121, 216, 288, 294
BslI CCNNNNNNNGG 5 cut(s) 137, 215, 436, 618, 704
BsmAI GTCTC 2 cut(s) 138, 146
BsmBI CGTCTC 1 cut(s) 138
BsmI GAATGC 1 cut(s) 400
BsnI GGCC 9 cut(s) 5, 160, 219, 228, 242, 297, 410, 458, 611
Bso31I GGTCTC 1 cut(s) 146
BsoBI CYCGRG 1 cut(s) 275
Bsp120I GGGCCC 1 cut(s) 226
Bsp1286I GDGCHC 3 cut(s) 230, 251, 282
Bsp13I TCCGGA 1 cut(s) 287
Bsp143I GATC 2 cut(s) 490, 523
Bsp19I CCATGG 1 cut(s) 703
BspANI GGCC 9 cut(s) 5, 160, 219, 228, 242, 297, 410, 458, 611
BspCNI CTCAG 1 cut(s) 435
BspEI TCCGGA 1 cut(s) 287
BspFNI CGCG 2 cut(s) 253, 413
BspLI GGNNCC 6 cut(s) 228, 229, 286, 292, 693, 709
BspOI GCTAGC 1 cut(s) 336
BspTNI GGTCTC 1 cut(s) 146
BsrBI CCGCTC 2 cut(s) 8, 265
BsrDI GCAATG 3 cut(s) 85, 789, 795
BsrFI RCCGGY 1 cut(s) 293
BssAI RCCGGY 1 cut(s) 293
BssECI CCNNGG 5 cut(s) 243, 459, 504, 703, 711
BssMI GATC 2 cut(s) 490, 523
BssT1I CCWWGG 2 cut(s) 243, 703
Bst2UI CCWGG 1 cut(s) 712
Bst4CI ACNGT 6 cut(s) 23, 112, 343, 699, 727, 734
BstAPI GCANNNNNTGC 1 cut(s) 779
BstBAI YACGTR 2 cut(s) 433, 688
BstC8I GCNNGC 4 cut(s) 162, 221, 251, 334
BstDEI CTNAG 2 cut(s) 44, 443
BstDSI CCRYGG 1 cut(s) 703
BstEII GGTNACC 1 cut(s) 343
BstF5I GGATG 2 cut(s) 117, 514
BstFNI CGCG 2 cut(s) 253, 413
BstH2I RGCGCY 1 cut(s) 443
BstHHI GCGC 1 cut(s) 442
BstKTI GATC 2 cut(s) 493, 526
BstMAI GTCTC 2 cut(s) 138, 146
BstMBI GATC 2 cut(s) 490, 523
BstMWI GCNNNNNNNGC 6 cut(s) 225, 255, 398, 407, 550, 779
BstNI CCWGG 1 cut(s) 712
BstPI GGTNACC 1 cut(s) 343
BstSCI CCNGG 1 cut(s) 710
BstSLI GKGCMC 1 cut(s) 230
BstUI CGCG 2 cut(s) 253, 413
BstV1I GCAGC 3 cut(s) 136, 323, 540
BstV2I GAAGAC 2 cut(s) 36, 106
BsuRI GGCC 9 cut(s) 5, 160, 219, 228, 242, 297, 410, 458, 611
BtgI CCRYGG 1 cut(s) 703
BtrI CACGTC 2 cut(s) 361, 466
BtsCI GGATG 2 cut(s) 117, 514
Cac8I GCNNGC 4 cut(s) 162, 221, 251, 334
CaiI CAGNNNCTG 1 cut(s) 317
CfoI GCGC 1 cut(s) 442
Cfr10I RCCGGY 1 cut(s) 293
Cfr13I GGNCC 5 cut(s) 218, 226, 227, 610, 691
CseI GACGC 2 cut(s) 242, 325
Csp6I GTAC 2 cut(s) 517, 532
CviAII CATG 1 cut(s) 704
CviQI GTAC 2 cut(s) 517, 532
DdeI CTNAG 2 cut(s) 44, 443
DpnI GATC 2 cut(s) 492, 525
DpnII GATC 2 cut(s) 490, 523
EaeI YGGCCR 3 cut(s) 3, 158, 408
EciI GGCGGA 1 cut(s) 599
Ecl136II GAGCTC 1 cut(s) 280
Eco130I CCWWGG 2 cut(s) 243, 703
Eco24I GRGCYC 3 cut(s) 230, 251, 282
Eco31I GGTCTC 1 cut(s) 146
Eco47I GGWCC 1 cut(s) 691
Eco53kI GAGCTC 1 cut(s) 280
Eco57I CTGAAG 1 cut(s) 48
Eco72I CACGTG 2 cut(s) 433, 688
Eco88I CYCGRG 1 cut(s) 275
Eco91I GGTNACC 1 cut(s) 343
EcoICRI GAGCTC 1 cut(s) 280
EcoO109I RGGNCCY 1 cut(s) 227
EcoO65I GGTNACC 1 cut(s) 343
EcoRII CCWGG 1 cut(s) 710
EcoT14I CCWWGG 2 cut(s) 243, 703
EcoT38I GRGCYC 3 cut(s) 230, 251, 282
ErhI CCWWGG 2 cut(s) 243, 703
Esp3I CGTCTC 1 cut(s) 138
FaeI CATG 1 cut(s) 707
FaiI YATR 1 cut(s) 705
FatI CATG 1 cut(s) 703
FauI CCCGC 5 cut(s) 228, 270, 363, 413, 474
FokI GGATG 2 cut(s) 104, 521
FriOI GRGCYC 3 cut(s) 230, 251, 282
FspBI CTAG 2 cut(s) 210, 333
GlaI GCGC 1 cut(s) 441
GsaI CCCAGC 1 cut(s) 227
GsuI CTGGAG 1 cut(s) 694
HaeII RGCGCY 1 cut(s) 443
HaeIII GGCC 9 cut(s) 5, 160, 219, 228, 242, 297, 410, 458, 611
HapII CCGG 4 cut(s) 121, 216, 288, 294
HgaI GACGC 2 cut(s) 242, 325
HhaI GCGC 1 cut(s) 442
Hin1II CATG 1 cut(s) 707
Hin6I GCGC 1 cut(s) 440
HinP1I GCGC 1 cut(s) 440
HindIII AAGCTT 1 cut(s) 662
HinfI GANTC 2 cut(s) 306, 635
HpaII CCGG 4 cut(s) 121, 216, 288, 294
HphI GGTGA 4 cut(s) 46, 337, 484, 518
Hpy166II GTNNAC 4 cut(s) 430, 517, 691, 730
Hpy188I TCNGA 4 cut(s) 28, 305, 329, 508
Hpy188III TCNNGA 2 cut(s) 288, 649
Hpy8I GTNNAC 4 cut(s) 430, 517, 691, 730
Hpy99I CGWCG 4 cut(s) 23, 194, 258, 305
HpyAV CCTTC 5 cut(s) 80, 157, 588, 625, 646
HpyCH4III ACNGT 6 cut(s) 23, 112, 343, 699, 727, 734
HpyCH4IV ACGT 6 cut(s) 173, 360, 384, 432, 465, 687
HpyCH4V TGCA 3 cut(s) 38, 685, 740
HpyF10VI GCNNNNNNNGC 6 cut(s) 225, 255, 398, 407, 550, 779
HpyF3I CTNAG 2 cut(s) 44, 443
HpySE526I ACGT 6 cut(s) 173, 360, 384, 432, 465, 687
Hsp92II CATG 1 cut(s) 707
HspAI GCGC 1 cut(s) 440
Kpn2I TCCGGA 1 cut(s) 287
Kzo9I GATC 2 cut(s) 490, 523
LmnI GCTCC 4 cut(s) 218, 290, 329, 713
Lsp1109I GCAGC 3 cut(s) 136, 323, 540
LweI GCATC 1 cut(s) 25
MaeI CTAG 2 cut(s) 210, 333
MaeII ACGT 6 cut(s) 173, 360, 384, 432, 465, 687
MaeIII GTNAC 6 cut(s) 52, 203, 319, 337, 343, 721
MalI GATC 2 cut(s) 492, 525
MbiI CCGCTC 2 cut(s) 8, 265
MboI GATC 2 cut(s) 490, 523
MboII GAAGA 4 cut(s) 41, 106, 637, 640
MhlI GDGCHC 3 cut(s) 230, 251, 282
MlsI TGGCCA 1 cut(s) 160
MluCI AATT 1 cut(s) 761
MluNI TGGCCA 1 cut(s) 160
MlyI GAGTC 1 cut(s) 629
MmeI TCCRAC 2 cut(s) 283, 697
Mox20I TGGCCA 1 cut(s) 160
MroI TCCGGA 1 cut(s) 287
MscI TGGCCA 1 cut(s) 160
MseI TTAA 1 cut(s) 381
Msp20I TGGCCA 1 cut(s) 160
MspA1I CMGCKG 2 cut(s) 223, 317
MspI CCGG 4 cut(s) 121, 216, 288, 294
MspR9I CCNGG 1 cut(s) 712
Mva1269I GAATGC 1 cut(s) 400
MvaI CCWGG 1 cut(s) 712
MvnI CGCG 2 cut(s) 253, 413
MwoI GCNNNNNNNGC 6 cut(s) 225, 255, 398, 407, 550, 779
NcoI CCATGG 1 cut(s) 703
NdeII GATC 2 cut(s) 490, 523
NheI GCTAGC 1 cut(s) 332
NlaIII CATG 1 cut(s) 707
NlaIV GGNNCC 6 cut(s) 228, 229, 286, 292, 693, 709
NmeAIII GCCGAG 3 cut(s) 261, 484, 617
NmuCI GTSAC 4 cut(s) 52, 319, 337, 343
PaeR7I CTCGAG 1 cut(s) 275
PcsI WCGNNNNNNNCGW 2 cut(s) 333, 423
PctI GAATGC 1 cut(s) 400
PfeI GAWTC 1 cut(s) 306
PleI GAGTC 1 cut(s) 629
PmaCI CACGTG 2 cut(s) 433, 688
PmlI CACGTG 2 cut(s) 433, 688
PpsI GAGTC 1 cut(s) 629
Ppu21I YACGTR 2 cut(s) 433, 688
Psp124BI GAGCTC 1 cut(s) 282
Psp6I CCWGG 1 cut(s) 710
PspCI CACGTG 2 cut(s) 433, 688
PspEI GGTNACC 1 cut(s) 343
PspFI CCCAGC 1 cut(s) 223
PspGI CCWGG 1 cut(s) 710
PspN4I GGNNCC 6 cut(s) 228, 229, 286, 292, 693, 709
PspOMI GGGCCC 1 cut(s) 226
PspPI GGNCC 5 cut(s) 218, 226, 227, 610, 691
PspXI VCTCGAGB 1 cut(s) 275
PstNI CAGNNNCTG 1 cut(s) 317
RsaI GTAC 2 cut(s) 518, 533
RsaNI GTAC 2 cut(s) 517, 532
SacI GAGCTC 1 cut(s) 282
SaqAI TTAA 1 cut(s) 381
Sau3AI GATC 2 cut(s) 490, 523
Sau96I GGNCC 5 cut(s) 218, 226, 227, 610, 691
SchI GAGTC 1 cut(s) 629
ScrFI CCNGG 1 cut(s) 712
SduI GDGCHC 3 cut(s) 230, 251, 282
SfaNI GCATC 1 cut(s) 25
SfiI GGCCNNNNNGGCC 1 cut(s) 225
Sfr274I CTCGAG 1 cut(s) 275
SinI GGWCC 1 cut(s) 691
SlaI CTCGAG 1 cut(s) 275
SmlI CTYRAG 3 cut(s) 135, 275, 616
SmoI CTYRAG 3 cut(s) 135, 275, 616
Sse9I AATT 1 cut(s) 761
SspMI CTAG 2 cut(s) 210, 333
SstI GAGCTC 1 cut(s) 282
StyD4I CCNGG 1 cut(s) 710
StyI CCWWGG 2 cut(s) 243, 703
TaaI ACNGT 6 cut(s) 23, 112, 343, 699, 727, 734
TaiI ACGT 6 cut(s) 176, 363, 387, 435, 468, 690
TaqI TCGA 2 cut(s) 189, 276
TasI AATT 1 cut(s) 761
TauI GCSGC 7 cut(s) 8, 62, 167, 317, 401, 413, 440
TfiI GAWTC 1 cut(s) 306
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TseFI GTSAC 4 cut(s) 52, 319, 337, 343
TseI GCWGC 3 cut(s) 124, 311, 553
Tsp45I GTSAC 4 cut(s) 52, 319, 337, 343
TspDTI ATGAA 1 cut(s) 106
TspGWI ACGGA 1 cut(s) 120
VpaK11BI GGWCC 1 cut(s) 691
XhoI CTCGAG 1 cut(s) 275
XspI CTAG 2 cut(s) 210, 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.