Rh2BG153500

Protein N-lysine methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0012035
Physical Location & Seq
Forward (+)
13154333 .. 13155154
822 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG153500.1

Sequence Viewer

Length: 822 bp
ATGGCCGCTCTAGAAGAAGACCAAGACGACGTCGTTATAAACCCCGCCACAATGCTCCTCCGCGACGACGACGACTCCGACGAGGTCAAGATGGCGGAGAGCGAACTGCTCCGGCAGCATTCTCTGCCTTCGATCGACTCCACAATCGTCACCAGGCAGCTCCCCTCGCAGGGTCTCTCCTTTCAGCTCTGGCCGGCGGCCACCACGCTCCTCACTCTCCTGGACGACCACCGCCGTGACCCCACCACGAGCCCCCTGGGCCCCACGCTCTCGGCCTTGGAATCCGGCCGGAGACTCAAGATCCTCGAACTAGGTTCTGGAACCGGCCTCGTCGGAATAGCCGCCGCTGCCACGCTCGGCGCGTGCGTCACTGTGACGGACCTCCCGCACGTCGTCGACAACCTTAAGTTCAATGCGGAACTAAACGCCGCCGCTTTGGGAGGGAGCGGCGGGAGTGTGAGAGTGGCGGCGCTGAGGTGGGGAGAGGCGGAGGACGCGGAGGCGATAGGGCGGGAGTTTGATATTGTATTGGCGTCGGATGTAGTGTATCACGACCACCTCTACGAGCCGCTGCTCAAAACGCTGTCGTTTCTGCTGTTGGGGGAGGGCGGCCTGGTGTTTGTGATGGCCCACTTGAGGAGGTGGAAGAAGGAGTCGGCCTTCTTCAAGAGGGCCAGGAAGCTTTTTGAGGTTGAGGTCTTGCACGTGGATCGTCCGTGCCAGGGATCGAGGGTCGGAGTTACTGTTTACCGTTTTACCGGGAAGCCAAGTAAGAAATTGTTGAATGGTGTCTCAGTGAACATTGTCAATGGCATTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

273

Amino Acids

29.6

Weight (kDa)

5.41

Isoelectric Point (pI)

39.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_16 PF10294 55 - 226 5.9e-31 Lysine methyltransferase
Methyltransf_23 PF13489 95 - 220 7e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012035)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50850 AT5G49560
fragaria_vesca FvH4_1g13310
malus_domestica MD02G1147500.v1.1 MD15G1261800.v1.1
prunus_persica Prupe.7G154600_v2.0.a1
pyrus_communis pycom15g22900
rosa_chinensis RchiOBHm_Chr2g0101571
rosa_laevigata RLG00000017091
rosa_multiflora Rmu_co8339275.1_g000001 Rmu_sc0006153.1_g000007 Rmu_sc0019613.1_g000002
rosa_roxburghii Rroxscaffold_2G00141180
rosa_rugosa Rorug02G0097300
rosa_samantha Rh2AG148100 Rh2BG153500 Rh2CG154000 Rh2DG153800
rosa_wichuraiana Rw2G011550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 38
AatII GACGTC 1 cut(s) 33
AccBSI CCGCTC 2 cut(s) 8, 447
AccI GTMKAC 1 cut(s) 396
AccII CGCG 3 cut(s) 63, 362, 497
AclWI GGATC 3 cut(s) 295, 717, 733
AcoI YGGCCR 4 cut(s) 3, 191, 198, 286
AcvI CACGTG 1 cut(s) 706
AcyI GRCGYC 2 cut(s) 30, 533
AfiI CCNNNNNNNGG 4 cut(s) 169, 170, 636, 722
AflII CTTAAG 1 cut(s) 404
AgsI TTSAA 3 cut(s) 412, 667, 784
AjiI CACGTC 1 cut(s) 391
AjnI CCWGG 6 cut(s) 152, 219, 255, 612, 674, 720
AleI CACNNNNGTG 1 cut(s) 234
AluBI AGCT 3 cut(s) 160, 187, 682
AluI AGCT 3 cut(s) 160, 187, 682
Alw26I GTCTC 3 cut(s) 179, 286, 796
AlwI GGATC 3 cut(s) 295, 717, 733
ApaI GGGCCC 1 cut(s) 263
ApeKI GCWGC 4 cut(s) 115, 157, 347, 571
AspLEI GCGC 2 cut(s) 362, 472
AspS9I GGNCC 5 cut(s) 259, 260, 379, 628, 672
AsuC2I CCSGG 1 cut(s) 760
AsuHPI GGTGA 1 cut(s) 142
AvaII GGWCC 1 cut(s) 379
BaeGI GKGCMC 1 cut(s) 263
BanII GRGCYC 2 cut(s) 254, 263
BauI CACGAG 1 cut(s) 247
BbrPI CACGTG 1 cut(s) 706
BbsI GAAGAC 1 cut(s) 24
BbvCI CCTCAGC 1 cut(s) 473
BbvI GCAGC 4 cut(s) 127, 169, 334, 558
BccI CCATC 2 cut(s) 85, 619
BceAI ACGGC 1 cut(s) 219
BcgI CGANNNNNNTGC 2 cut(s) 692, 726
BciT130I CCWGG 6 cut(s) 154, 221, 257, 614, 676, 722
BcnI CCSGG 1 cut(s) 760
BcoDI GTCTC 3 cut(s) 179, 286, 796
BfaI CTAG 2 cut(s) 11, 311
BfoI RGCGCY 1 cut(s) 473
BfrI CTTAAG 1 cut(s) 404
BglI GCCNNNNNGGC 1 cut(s) 258
Bme1390I CCNGG 7 cut(s) 154, 221, 257, 614, 676, 722, 760
Bme18I GGWCC 1 cut(s) 379
BmgBI CACGTC 1 cut(s) 391
BmgT120I GGNCC 5 cut(s) 259, 260, 379, 628, 672
BmiI GGNNCC 3 cut(s) 261, 262, 322
BmrFI CCNGG 7 cut(s) 154, 221, 257, 614, 676, 722, 760
BpiI GAAGAC 1 cut(s) 24
Bpu10I CCTNAGC 1 cut(s) 473
BpuEI CTTGAG 2 cut(s) 281, 655
BpuMI CCSGG 1 cut(s) 760
BsaAI YACGTR 1 cut(s) 706
BsaHI GRCGYC 2 cut(s) 30, 533
BsaI GGTCTC 1 cut(s) 179
BsaJI CCNNGG 4 cut(s) 255, 256, 276, 721
BsaXI ACNNNNNCTCC 4 cut(s) 59, 89, 729, 759
Bsc4I CCNNNNNNNGG 4 cut(s) 169, 170, 636, 722
Bse118I RCCGGY 2 cut(s) 193, 323
BseBI CCWGG 6 cut(s) 154, 221, 257, 614, 676, 722
BseDI CCNNGG 4 cut(s) 255, 256, 276, 721
BseGI GGATG 1 cut(s) 544
BseLI CCNNNNNNNGG 4 cut(s) 169, 170, 636, 722
BseMII CTCAG 2 cut(s) 464, 807
BseRI GAGGAG 3 cut(s) 47, 200, 652
BseSI GKGCMC 1 cut(s) 263
BseX3I CGGCCG 1 cut(s) 286
BseXI GCAGC 4 cut(s) 127, 169, 334, 558
Bsh1236I CGCG 3 cut(s) 63, 362, 497
Bsh1285I CGRYCG 2 cut(s) 135, 289
BsiEI CGRYCG 2 cut(s) 135, 289
BsiSI CCGG 6 cut(s) 112, 194, 285, 289, 324, 759
BslI CCNNNNNNNGG 4 cut(s) 169, 170, 636, 722
BsmAI GTCTC 3 cut(s) 179, 286, 796
BsmI GAATGC 2 cut(s) 118, 813
Bso31I GGTCTC 1 cut(s) 179
Bsp120I GGGCCC 1 cut(s) 259
Bsp1286I GDGCHC 2 cut(s) 254, 263
Bsp143I GATC 4 cut(s) 132, 300, 709, 725
BspCNI CTCAG 2 cut(s) 465, 806
BspFNI CGCG 3 cut(s) 63, 362, 497
BspLI GGNNCC 3 cut(s) 261, 262, 322
BspPI GGATC 3 cut(s) 295, 717, 733
BspTI CTTAAG 1 cut(s) 404
BspTNI GGTCTC 1 cut(s) 179
BsrBI CCGCTC 2 cut(s) 8, 447
BsrFI RCCGGY 2 cut(s) 193, 323
BssAI RCCGGY 2 cut(s) 193, 323
BssECI CCNNGG 4 cut(s) 255, 256, 276, 721
BssMI GATC 4 cut(s) 132, 300, 709, 725
BssNI GRCGYC 2 cut(s) 30, 533
BssSI CACGAG 1 cut(s) 247
BssT1I CCWWGG 1 cut(s) 276
Bst2BI CACGAG 1 cut(s) 247
Bst2UI CCWGG 6 cut(s) 154, 221, 257, 614, 676, 722
Bst4CI ACNGT 3 cut(s) 373, 745, 752
BstACI GRCGYC 2 cut(s) 30, 533
BstAFI CTTAAG 1 cut(s) 404
BstAPI GCANNNNNTGC 1 cut(s) 124
BstBAI YACGTR 1 cut(s) 706
BstC8I GCNNGC 2 cut(s) 195, 364
BstDEI CTNAG 2 cut(s) 473, 793
BstF5I GGATG 1 cut(s) 544
BstFNI CGCG 3 cut(s) 63, 362, 497
BstH2I RGCGCY 1 cut(s) 473
BstHHI GCGC 2 cut(s) 362, 472
BstKTI GATC 4 cut(s) 135, 303, 712, 728
BstMAI GTCTC 3 cut(s) 179, 286, 796
BstMBI GATC 4 cut(s) 132, 300, 709, 725
BstMCI CGRYCG 2 cut(s) 135, 289
BstMWI GCNNNNNNNGC 7 cut(s) 115, 124, 166, 258, 347, 494, 580
BstNI CCWGG 6 cut(s) 154, 221, 257, 614, 676, 722
BstSCI CCNGG 7 cut(s) 152, 219, 255, 612, 674, 720, 758
BstSLI GKGCMC 1 cut(s) 263
BstUI CGCG 3 cut(s) 63, 362, 497
BstV1I GCAGC 4 cut(s) 127, 169, 334, 558
BstV2I GAAGAC 1 cut(s) 24
BstX2I RGATCY 1 cut(s) 300
BstYI RGATCY 1 cut(s) 300
BstZI CGGCCG 1 cut(s) 286
BtrI CACGTC 1 cut(s) 391
BtsCI GGATG 1 cut(s) 544
BtsIMutI CAGTG 2 cut(s) 369, 801
Cac8I GCNNGC 2 cut(s) 195, 364
CfoI GCGC 2 cut(s) 362, 472
Cfr10I RCCGGY 2 cut(s) 193, 323
Cfr13I GGNCC 5 cut(s) 259, 260, 379, 628, 672
CseI GACGC 3 cut(s) 355, 503, 522
DdeI CTNAG 2 cut(s) 473, 793
DpnI GATC 4 cut(s) 134, 302, 711, 727
DpnII GATC 4 cut(s) 132, 300, 709, 725
EaeI YGGCCR 4 cut(s) 3, 191, 198, 286
EagI CGGCCG 1 cut(s) 286
EciI GGCGGA 2 cut(s) 110, 503
EclXI CGGCCG 1 cut(s) 286
Eco130I CCWWGG 1 cut(s) 276
Eco24I GRGCYC 2 cut(s) 254, 263
Eco31I GGTCTC 1 cut(s) 179
Eco47I GGWCC 1 cut(s) 379
Eco52I CGGCCG 1 cut(s) 286
Eco72I CACGTG 1 cut(s) 706
EcoO109I RGGNCCY 1 cut(s) 260
EcoRII CCWGG 6 cut(s) 152, 219, 255, 612, 674, 720
EcoT14I CCWWGG 1 cut(s) 276
EcoT38I GRGCYC 2 cut(s) 254, 263
ErhI CCWWGG 1 cut(s) 276
FaiI YATR 1 cut(s) 38
FauI CCCGC 4 cut(s) 52, 393, 443, 504
FblI GTMKAC 1 cut(s) 396
FokI GGATG 1 cut(s) 551
FriOI GRGCYC 2 cut(s) 254, 263
FspBI CTAG 2 cut(s) 11, 311
GlaI GCGC 2 cut(s) 361, 471
HaeII RGCGCY 1 cut(s) 473
HapII CCGG 6 cut(s) 112, 194, 285, 289, 324, 759
HgaI GACGC 3 cut(s) 355, 503, 522
HhaI GCGC 2 cut(s) 362, 472
Hin1I GRCGYC 2 cut(s) 30, 533
Hin6I GCGC 2 cut(s) 360, 470
HinP1I GCGC 2 cut(s) 360, 470
HincII GTYRAC 1 cut(s) 397
HindII GTYRAC 1 cut(s) 397
HindIII AAGCTT 1 cut(s) 680
HinfI GANTC 5 cut(s) 74, 137, 281, 294, 653
HpaII CCGG 6 cut(s) 112, 194, 285, 289, 324, 759
HphI GGTGA 1 cut(s) 142
Hpy166II GTNNAC 3 cut(s) 397, 748, 799
Hpy188I TCNGA 4 cut(s) 79, 335, 538, 737
Hpy188III TCNNGA 6 cut(s) 11, 88, 298, 318, 551, 667
Hpy8I GTNNAC 3 cut(s) 397, 748, 799
HpyAV CCTTC 3 cut(s) 138, 643, 670
HpyCH4III ACNGT 3 cut(s) 373, 745, 752
HpyCH4IV ACGT 3 cut(s) 30, 390, 705
HpyCH4V TGCA 1 cut(s) 703
HpyF10VI GCNNNNNNNGC 7 cut(s) 115, 124, 166, 258, 347, 494, 580
HpyF3I CTNAG 2 cut(s) 473, 793
HpySE526I ACGT 3 cut(s) 30, 390, 705
Hsp92I GRCGYC 2 cut(s) 30, 533
HspAI GCGC 2 cut(s) 360, 470
KroI GCCGGC 1 cut(s) 193
KroNI GCCGGC 1 cut(s) 195
Kzo9I GATC 4 cut(s) 132, 300, 709, 725
LmnI GCTCC 5 cut(s) 60, 114, 165, 213, 444
Lsp1109I GCAGC 4 cut(s) 127, 169, 334, 558
MaeI CTAG 2 cut(s) 11, 311
MaeII ACGT 3 cut(s) 30, 390, 705
MaeIII GTNAC 5 cut(s) 148, 236, 367, 373, 739
MalI GATC 4 cut(s) 134, 302, 711, 727
MbiI CCGCTC 2 cut(s) 8, 447
MboI GATC 4 cut(s) 132, 300, 709, 725
MboII GAAGA 4 cut(s) 26, 29, 655, 658
MflI RGATCY 1 cut(s) 300
MhlI GDGCHC 2 cut(s) 254, 263
MluCI AATT 1 cut(s) 776
MlyI GAGTC 4 cut(s) 68, 131, 288, 662
MmeI TCCRAC 4 cut(s) 102, 313, 516, 715
MroNI GCCGGC 1 cut(s) 193
MseI TTAA 1 cut(s) 405
MslI CAYNNNNRTG 1 cut(s) 234
MspA1I CMGCKG 2 cut(s) 347, 571
MspCI CTTAAG 1 cut(s) 404
MspI CCGG 6 cut(s) 112, 194, 285, 289, 324, 759
MspR9I CCNGG 7 cut(s) 154, 221, 257, 614, 676, 722, 760
Mva1269I GAATGC 2 cut(s) 118, 813
MvaI CCWGG 6 cut(s) 154, 221, 257, 614, 676, 722
MvnI CGCG 3 cut(s) 63, 362, 497
MwoI GCNNNNNNNGC 7 cut(s) 115, 124, 166, 258, 347, 494, 580
NaeI GCCGGC 1 cut(s) 195
NciI CCSGG 1 cut(s) 760
NdeII GATC 4 cut(s) 132, 300, 709, 725
NgoMIV GCCGGC 1 cut(s) 193
NlaIV GGNNCC 3 cut(s) 261, 262, 322
NmeAIII GCCGAG 2 cut(s) 251, 336
NmuCI GTSAC 4 cut(s) 148, 236, 367, 373
OliI CACNNNNGTG 1 cut(s) 234
PasI CCCWGGG 1 cut(s) 256
PcsI WCGNNNNNNNCGW 4 cut(s) 75, 78, 359, 363
PctI GAATGC 2 cut(s) 118, 813
PdiI GCCGGC 1 cut(s) 195
PfeI GAWTC 1 cut(s) 281
PflFI GACNNNGTC 2 cut(s) 29, 83
PfoI TCCNGGA 1 cut(s) 219
Ple19I CGATCG 1 cut(s) 135
PleI GAGTC 4 cut(s) 68, 131, 288, 661
PmaCI CACGTG 1 cut(s) 706
PmlI CACGTG 1 cut(s) 706
PpsI GAGTC 4 cut(s) 68, 131, 288, 661
Ppu21I YACGTR 1 cut(s) 706
PsiI TTATAA 1 cut(s) 38
Psp6I CCWGG 6 cut(s) 152, 219, 255, 612, 674, 720
PspCI CACGTG 1 cut(s) 706
PspGI CCWGG 6 cut(s) 152, 219, 255, 612, 674, 720
PspN4I GGNNCC 3 cut(s) 261, 262, 322
PspOMI GGGCCC 1 cut(s) 259
PspPI GGNCC 5 cut(s) 259, 260, 379, 628, 672
PsuI RGATCY 1 cut(s) 300
PsyI GACNNNGTC 2 cut(s) 29, 83
PvuI CGATCG 1 cut(s) 135
RseI CAYNNNNRTG 1 cut(s) 234
SalI GTCGAC 1 cut(s) 395
SaqAI TTAA 1 cut(s) 405
Sau3AI GATC 4 cut(s) 132, 300, 709, 725
Sau96I GGNCC 5 cut(s) 259, 260, 379, 628, 672
SchI GAGTC 4 cut(s) 68, 131, 288, 662
ScrFI CCNGG 7 cut(s) 154, 221, 257, 614, 676, 722, 760
SduI GDGCHC 2 cut(s) 254, 263
SinI GGWCC 1 cut(s) 379
SmiMI CAYNNNNRTG 1 cut(s) 234
SmlI CTYRAG 3 cut(s) 296, 404, 634
SmoI CTYRAG 3 cut(s) 296, 404, 634
Sse9I AATT 1 cut(s) 776
SspMI CTAG 2 cut(s) 11, 311
StyD4I CCNGG 7 cut(s) 152, 219, 255, 612, 674, 720, 758
StyI CCWWGG 1 cut(s) 276
TaaI ACNGT 3 cut(s) 373, 745, 752
TaiI ACGT 3 cut(s) 33, 393, 708
TaqI TCGA 5 cut(s) 131, 135, 306, 396, 728
TasI AATT 1 cut(s) 776
TfiI GAWTC 1 cut(s) 281
Tru1I TTAA 1 cut(s) 405
Tru9I TTAA 1 cut(s) 405
TscAI CASTG 2 cut(s) 376, 801
TseFI GTSAC 4 cut(s) 148, 236, 367, 373
TseI GCWGC 4 cut(s) 115, 157, 347, 571
Tsp45I GTSAC 4 cut(s) 148, 236, 367, 373
TspGWI ACGGA 2 cut(s) 392, 705
TspRI CASTG 2 cut(s) 376, 801
Tth111I GACNNNGTC 2 cut(s) 29, 83
Vha464I CTTAAG 1 cut(s) 404
VpaK11BI GGWCC 1 cut(s) 379
XbaI TCTAGA 1 cut(s) 10
XcmI CCANNNNNNNNNTGG 1 cut(s) 253
XmiI GTMKAC 1 cut(s) 396
XspI CTAG 2 cut(s) 11, 311
ZraI GACGTC 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.