AT5G49560

Protein N-lysine methyltransferase METTL21A-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012035
Physical Location & Seq
Reverse (-)
20110832 .. 20111806
975 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G49560.1

Sequence Viewer

Length: 825 bp
ATGATTACTCTTCCTCTGCGAGACGACGATGAGGAAAACGACACATACGAAGCTAAAATGCTCCTCATAGGCGAGAATGGTCTCGACGGTGAGAGTTTACCTCCTACTCCTCTGCGTGACGGTGCACTGGAGCTCCAGAAGTATAACATCAGCTCCATCGAGTCAACAGTCGTGATTCGTGAGCTGACGTCACAAGGTCTCTCCTTTCAGCTCTGGCCAGCGGCTTCTACTTTCGTCACGTTGCTTGATAACTACCGACGTGACCCTAGCAAGAGTCCACTCACCGCCACACTCTTGTCGCTGAAAAAACCATCGCCGTTGAACATACTCGAGCTCGGATCTGGAACCGGCCTCGTCGGGATCGCTGCGGCAATCACTCTCTCTGCTAACGTCACGGTGACGGATCTCCCACACGTCTTAGATAATCTCAACTTCAACGCCGAAGCAAACGCTGAAATTGTTGAGAGATTCGGCGGGAAAGTCAACGTGGCACCACTACGATGGGGAGAAGCTGATGACGTGGAGGTTCTAGGTCAGAACGTTGACTTGATTCTGGCTTCGGACGTGGTGTATCATGACCATCTCTACGAGCCTCTCCTCAAAACGCTGCGTTTGATGCAGCTAGAGGGGAAGAGGTTAATTTTCCTGATGGCGCATCTAAGGAGATGGAAGAAAGAATCTGTCTTCTTCAAAAAAGCTCGAAAGCTCTTCGATGTTGACGTTATCCACTCTGATGTTCCTCAAGAAAGTTCTAGAATCGGTGTTGTGGTTTACCGTTTCACCACGAAACAGCCAAATCAGAATGGTCGGATTGTTTCGTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

274

Amino Acids

30.49

Weight (kDa)

5.34

Isoelectric Point (pI)

43.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_16 PF10294 55 - 234 1.1e-32 Lysine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012035)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50850 AT5G49560
fragaria_vesca FvH4_1g13310
malus_domestica MD02G1147500.v1.1 MD15G1261800.v1.1
prunus_persica Prupe.7G154600_v2.0.a1
pyrus_communis pycom15g22900
rosa_chinensis RchiOBHm_Chr2g0101571
rosa_laevigata RLG00000017091
rosa_multiflora Rmu_co8339275.1_g000001 Rmu_sc0006153.1_g000007 Rmu_sc0019613.1_g000002
rosa_roxburghii Rroxscaffold_2G00141180
rosa_rugosa Rorug02G0097300
rosa_samantha Rh2AG148100 Rh2BG153500 Rh2CG154000 Rh2DG153800
rosa_wichuraiana Rw2G011550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 191
AccB1I GGYRCC 1 cut(s) 490
AciI CCGC 4 cut(s) 221, 285, 368, 474
AclI AACGTT 1 cut(s) 540
AclWI GGATC 3 cut(s) 346, 368, 411
AcoI YGGCCR 1 cut(s) 215
AcyI GRCGYC 1 cut(s) 188
AflIII ACRYGT 1 cut(s) 412
AgsI TTSAA 3 cut(s) 322, 436, 691
AjiI CACGTC 4 cut(s) 260, 415, 520, 565
Alw21I GWGCWC 3 cut(s) 127, 135, 336
Alw26I GTCTC 3 cut(s) 15, 86, 203
Alw44I GTGCAC 1 cut(s) 123
AlwI GGATC 3 cut(s) 346, 368, 411
Ama87I CYCGRG 1 cut(s) 329
AoxI GGCC 2 cut(s) 215, 349
ApaLI GTGCAC 1 cut(s) 123
ApeKI GCWGC 3 cut(s) 365, 607, 619
AspLEI GCGC 1 cut(s) 655
AsuHPI GGTGA 4 cut(s) 101, 274, 409, 772
AvaI CYCGRG 1 cut(s) 329
BaeGI GKGCMC 1 cut(s) 127
BalI TGGCCA 1 cut(s) 217
BanI GGYRCC 1 cut(s) 490
BanII GRGCYC 2 cut(s) 135, 336
BbsI GAAGAC 1 cut(s) 676
Bbv12I GWGCWC 3 cut(s) 127, 135, 336
BbvI GCAGC 3 cut(s) 352, 594, 631
BccI CCATC 6 cut(s) 164, 319, 495, 588, 643, 660
BceAI ACGGC 1 cut(s) 301
BcoDI GTCTC 3 cut(s) 15, 86, 203
BfaI CTAG 4 cut(s) 267, 530, 623, 753
BisI GCNGC 5 cut(s) 222, 366, 369, 608, 620
BlsI GCNGC 5 cut(s) 223, 367, 370, 609, 621
BmeT110I CYCGRG 1 cut(s) 329
BmgBI CACGTC 4 cut(s) 260, 415, 520, 565
BmiI GGNNCC 2 cut(s) 346, 492
BmsI GCATC 2 cut(s) 606, 664
BpiI GAAGAC 1 cut(s) 676
BplI GAGNNNNNCTC 2 cut(s) 85, 117
BpmI CTGGAG 2 cut(s) 119, 149
BpuEI CTTGAG 1 cut(s) 726
BsaHI GRCGYC 1 cut(s) 188
BsaI GGTCTC 2 cut(s) 86, 203
BsaXI ACNNNNNCTCC 4 cut(s) 117, 137, 147, 167
Bse118I RCCGGY 1 cut(s) 347
Bse1I ACTGG 1 cut(s) 132
BseNI ACTGG 1 cut(s) 132
BseRI GAGGAG 3 cut(s) 53, 99, 587
BseSI GKGCMC 1 cut(s) 127
BseXI GCAGC 3 cut(s) 352, 594, 631
BshFI GGCC 2 cut(s) 217, 351
BshNI GGYRCC 1 cut(s) 490
BsiHKAI GWGCWC 3 cut(s) 127, 135, 336
BsiHKCI CYCGRG 1 cut(s) 329
BsiSI CCGG 1 cut(s) 348
BsmAI GTCTC 3 cut(s) 15, 86, 203
BsmBI CGTCTC 1 cut(s) 15
BsnI GGCC 2 cut(s) 217, 351
Bso31I GGTCTC 2 cut(s) 86, 203
BsoBI CYCGRG 1 cut(s) 329
Bsp1286I GDGCHC 3 cut(s) 127, 135, 336
Bsp143I GATC 3 cut(s) 338, 360, 403
BspACI CCGC 4 cut(s) 221, 285, 368, 474
BspANI GGCC 2 cut(s) 217, 351
BspHI TCATGA 1 cut(s) 574
BspLI GGNNCC 2 cut(s) 346, 492
BspPI GGATC 3 cut(s) 346, 368, 411
BspQI GCTCTTC 1 cut(s) 713
BspT107I GGYRCC 1 cut(s) 490
BspTNI GGTCTC 2 cut(s) 86, 203
BsrFI RCCGGY 1 cut(s) 347
BsrI ACTGG 1 cut(s) 132
BssAI RCCGGY 1 cut(s) 347
BssMI GATC 3 cut(s) 338, 360, 403
BssNI GRCGYC 1 cut(s) 188
Bst4CI ACNGT 5 cut(s) 89, 122, 169, 397, 776
Bst6I CTCTTC 3 cut(s) 15, 626, 713
BstACI GRCGYC 1 cut(s) 188
BstC8I GCNNGC 1 cut(s) 219
BstDEI CTNAG 2 cut(s) 418, 659
BstHHI GCGC 1 cut(s) 655
BstKTI GATC 3 cut(s) 341, 363, 406
BstMAI GTCTC 3 cut(s) 15, 86, 203
BstMBI GATC 3 cut(s) 338, 360, 403
BstMWI GCNNNNNNNGC 1 cut(s) 616
BstSLI GKGCMC 1 cut(s) 127
BstV1I GCAGC 3 cut(s) 352, 594, 631
BstV2I GAAGAC 1 cut(s) 676
BstX2I RGATCY 2 cut(s) 338, 403
BstXI CCANNNNNNTGG 1 cut(s) 501
BstYI RGATCY 2 cut(s) 338, 403
BsuRI GGCC 2 cut(s) 217, 351
BtgZI GCGATG 1 cut(s) 297
BtrI CACGTC 4 cut(s) 260, 415, 520, 565
BtsIMutI CAGTG 1 cut(s) 125
Cac8I GCNNGC 1 cut(s) 219
CciI TCATGA 1 cut(s) 574
CfoI GCGC 1 cut(s) 655
Cfr10I RCCGGY 1 cut(s) 347
CviAII CATG 1 cut(s) 575
DdeI CTNAG 2 cut(s) 418, 659
DpnI GATC 3 cut(s) 340, 362, 405
DpnII GATC 3 cut(s) 338, 360, 403
EaeI YGGCCR 1 cut(s) 215
Eam1104I CTCTTC 3 cut(s) 15, 626, 713
EarI CTCTTC 3 cut(s) 15, 626, 713
Ecl136II GAGCTC 2 cut(s) 133, 334
Eco24I GRGCYC 2 cut(s) 135, 336
Eco31I GGTCTC 2 cut(s) 86, 203
Eco53kI GAGCTC 2 cut(s) 133, 334
Eco88I CYCGRG 1 cut(s) 329
EcoICRI GAGCTC 2 cut(s) 133, 334
EcoT38I GRGCYC 2 cut(s) 135, 336
Esp3I CGTCTC 1 cut(s) 15
FaeI CATG 1 cut(s) 578
FaiI YATR 5 cut(s) 46, 68, 144, 326, 576
FatI CATG 1 cut(s) 574
FauI CCCGC 1 cut(s) 467
Fnu4HI GCNGC 5 cut(s) 222, 366, 369, 608, 620
FriOI GRGCYC 2 cut(s) 135, 336
Fsp4HI GCNGC 5 cut(s) 222, 366, 369, 608, 620
FspBI CTAG 4 cut(s) 267, 530, 623, 753
GlaI GCGC 1 cut(s) 654
GluI GCNGC 5 cut(s) 222, 366, 369, 608, 620
GsuI CTGGAG 2 cut(s) 119, 149
HaeIII GGCC 2 cut(s) 217, 351
HapII CCGG 1 cut(s) 348
HhaI GCGC 1 cut(s) 655
Hin1I GRCGYC 1 cut(s) 188
Hin1II CATG 1 cut(s) 578
Hin6I GCGC 1 cut(s) 653
HinP1I GCGC 1 cut(s) 653
HincII GTYRAC 4 cut(s) 165, 484, 544, 718
HindII GTYRAC 4 cut(s) 165, 484, 544, 718
HinfI GANTC 7 cut(s) 161, 175, 274, 468, 550, 677, 756
HpaII CCGG 1 cut(s) 348
HphI GGTGA 4 cut(s) 101, 274, 409, 772
Hpy166II GTNNAC 8 cut(s) 98, 125, 165, 278, 484, 544, 718, 772
Hpy188I TCNGA 6 cut(s) 338, 537, 562, 733, 801, 810
Hpy8I GTNNAC 8 cut(s) 98, 125, 165, 278, 484, 544, 718, 772
Hpy99I CGWCG 4 cut(s) 29, 89, 261, 359
HpyCH4III ACNGT 5 cut(s) 89, 122, 169, 397, 776
HpyCH4V TGCA 2 cut(s) 125, 619
HpyF10VI GCNNNNNNNGC 1 cut(s) 616
HpyF3I CTNAG 2 cut(s) 418, 659
Hsp92I GRCGYC 1 cut(s) 188
Hsp92II CATG 1 cut(s) 578
HspAI GCGC 1 cut(s) 653
Kzo9I GATC 3 cut(s) 338, 360, 403
LguI GCTCTTC 1 cut(s) 713
LmnI GCTCC 4 cut(s) 66, 130, 138, 158
LpnPI CCDG 8 cut(s) 113, 149, 199, 231, 327, 361, 539, 659
Lsp1109I GCAGC 3 cut(s) 352, 594, 631
LweI GCATC 2 cut(s) 606, 664
MaeI CTAG 4 cut(s) 267, 530, 623, 753
MaeIII GTNAC 6 cut(s) 116, 189, 235, 260, 391, 397
MalI GATC 3 cut(s) 340, 362, 405
MboI GATC 3 cut(s) 338, 360, 403
MboII GAAGA 5 cut(s) 643, 676, 679, 682, 700
MflI RGATCY 2 cut(s) 338, 403
MhlI GDGCHC 3 cut(s) 127, 135, 336
MlsI TGGCCA 1 cut(s) 217
MluCI AATT 2 cut(s) 456, 639
MluNI TGGCCA 1 cut(s) 217
MlyI GAGTC 2 cut(s) 170, 283
MmeI TCCRAC 1 cut(s) 788
Mox20I TGGCCA 1 cut(s) 217
MscI TGGCCA 1 cut(s) 217
MseI TTAA 1 cut(s) 638
MslI CAYNNNNRTG 2 cut(s) 499, 732
Msp20I TGGCCA 1 cut(s) 217
MspA1I CMGCKG 1 cut(s) 221
MspI CCGG 1 cut(s) 348
MwoI GCNNNNNNNGC 1 cut(s) 616
NdeII GATC 3 cut(s) 338, 360, 403
NlaIII CATG 1 cut(s) 578
NlaIV GGNNCC 2 cut(s) 346, 492
NmuCI GTSAC 6 cut(s) 116, 189, 235, 260, 391, 397
PaeR7I CTCGAG 1 cut(s) 329
PagI TCATGA 1 cut(s) 574
PciSI GCTCTTC 1 cut(s) 713
PcsI WCGNNNNNNNCGW 2 cut(s) 45, 717
PfeI GAWTC 5 cut(s) 175, 468, 550, 677, 756
PkrI GCNGC 5 cut(s) 223, 367, 370, 609, 621
PleI GAGTC 2 cut(s) 169, 282
PpsI GAGTC 2 cut(s) 169, 282
Psp124BI GAGCTC 2 cut(s) 135, 336
Psp1406I AACGTT 1 cut(s) 540
PspN4I GGNNCC 2 cut(s) 346, 492
PspXI VCTCGAGB 1 cut(s) 329
PsuI RGATCY 2 cut(s) 338, 403
RseI CAYNNNNRTG 2 cut(s) 499, 732
SacI GAGCTC 2 cut(s) 135, 336
SapI GCTCTTC 1 cut(s) 713
SaqAI TTAA 1 cut(s) 638
SatI GCNGC 5 cut(s) 222, 366, 369, 608, 620
Sau3AI GATC 3 cut(s) 338, 360, 403
SchI GAGTC 2 cut(s) 170, 283
SduI GDGCHC 3 cut(s) 127, 135, 336
SfaNI GCATC 2 cut(s) 606, 664
Sfr274I CTCGAG 1 cut(s) 329
SlaI CTCGAG 1 cut(s) 329
SmiMI CAYNNNNRTG 2 cut(s) 499, 732
SmlI CTYRAG 2 cut(s) 329, 741
SmoI CTYRAG 2 cut(s) 329, 741
Sse9I AATT 2 cut(s) 456, 639
SsiI CCGC 4 cut(s) 221, 285, 368, 474
SspMI CTAG 4 cut(s) 267, 530, 623, 753
SstI GAGCTC 2 cut(s) 135, 336
TaaI ACNGT 5 cut(s) 89, 122, 169, 397, 776
TaqI TCGA 5 cut(s) 84, 159, 330, 700, 711
TasI AATT 2 cut(s) 456, 639
TauI GCSGC 2 cut(s) 224, 371
TfiI GAWTC 5 cut(s) 175, 468, 550, 677, 756
Tru1I TTAA 1 cut(s) 638
Tru9I TTAA 1 cut(s) 638
TscAI CASTG 1 cut(s) 132
TseFI GTSAC 6 cut(s) 116, 189, 235, 260, 391, 397
TseI GCWGC 3 cut(s) 365, 607, 619
Tsp45I GTSAC 6 cut(s) 116, 189, 235, 260, 391, 397
TspGWI ACGGA 1 cut(s) 416
TspRI CASTG 1 cut(s) 132
VneI GTGCAC 1 cut(s) 123
XbaI TCTAGA 1 cut(s) 752
XhoI CTCGAG 1 cut(s) 329
XspI CTAG 4 cut(s) 267, 530, 623, 753
ZraI GACGTC 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.