MD15G1261800.v1.1

Protein N-lysine methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012035
Physical Location & Seq
Forward (+)
22375618 .. 22376418
801 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1261800.v1.1.491

Sequence Viewer

Length: 801 bp
ATGGCGGATCAAGAAGGTGGTGATTCGGAAGACGATGCAATACTGATCCCCGAGACCGCAACGCTCCTTCCATCGACTCAACATTGCCTCCACTCCATCAACTCAGTCGTCTGTATCCGGGAGCTCCGGTCTCAAGGCCTCTCTTTCCAGCTTTGGCCGGCCGCCACCACGCTCCTCACTCTCCTCGACGGCTACCACGGTGACCCTAGCACCAGTCCCCTGGGACCCACACTCTCGGCCTTGGACATGCGGAAGCGCCCTCTCAAAATCCTCGAGCTCGGCTCCGGAACCGGCCTCGTCGGAATCGCCGCCGCAGCCACGCTCGGGGCTACCGTCACGGTCACTGACCTCCCACACGTCATCCCTAACCTTTTATTCAACGTGGAAGCGAACGTCTCCGTATTGGAAGCCAACGGCGGCATTGTCCACGTGGCGGCGCTGGGGTGGGGGGAAGCGGCGGACGTGGAGGTGATTGGGCGGGAATTTGATCTTATTCTGGCGTCGGATGTAGTGTATCACGATCATCTCTACGGGCCGTTGCTCAAAACGTTGCGTTTGCTGATATTAGGTGAGGCGGAGGAGAATGAGGAGGGAAAGGTTTTCGTGATGGCCCACCTGAGGCGGTGGAAGAAGGAGTCGGCTTTCTTCAAGAAGGCCAGGAAGGTTTTTGAGGTGGAGGTCTTGCACGTGGACCCCCCGTGCCATGGCTCCAGGGTTGGAGTTACTGTTTTCCGTTTTACCGCGAAGAAGTCGGGTCGGAAAATGTTGAATTCTACCGGCAATACCAATGTCACGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

267

Amino Acids

28.66

Weight (kDa)

5.8

Isoelectric Point (pI)

31.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_16 PF10294 37 - 220 8.9e-30 Lysine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012035)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50850 AT5G49560
fragaria_vesca FvH4_1g13310
malus_domestica MD02G1147500.v1.1 MD15G1261800.v1.1
prunus_persica Prupe.7G154600_v2.0.a1
pyrus_communis pycom15g22900
rosa_chinensis RchiOBHm_Chr2g0101571
rosa_laevigata RLG00000017091
rosa_multiflora Rmu_co8339275.1_g000001 Rmu_sc0006153.1_g000007 Rmu_sc0019613.1_g000002
rosa_roxburghii Rroxscaffold_2G00141180
rosa_rugosa Rorug02G0097300
rosa_samantha Rh2AG148100 Rh2BG153500 Rh2CG154000 Rh2DG153800
rosa_wichuraiana Rw2G011550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 743
AccIII TCCGGA 1 cut(s) 284
AclI AACGTT 1 cut(s) 548
AclWI GGATC 2 cut(s) 15, 40
AcoI YGGCCR 2 cut(s) 155, 159
AcsI RAATTY 2 cut(s) 482, 769
AcvI CACGTG 2 cut(s) 430, 688
AcyI GRCGYC 1 cut(s) 500
AfiI CCNNNNNNNGG 4 cut(s) 324, 433, 618, 704
AflIII ACRYGT 1 cut(s) 355
AgsI TTSAA 3 cut(s) 379, 649, 769
AjiI CACGTC 2 cut(s) 358, 463
AjnI CCWGG 3 cut(s) 219, 656, 710
AluBI AGCT 3 cut(s) 124, 151, 277
AluI AGCT 3 cut(s) 124, 151, 277
Alw21I GWGCWC 2 cut(s) 126, 279
Alw26I GTCTC 3 cut(s) 47, 135, 400
AlwI GGATC 2 cut(s) 15, 40
Ama87I CYCGRG 3 cut(s) 50, 272, 323
Aor13HI TCCGGA 1 cut(s) 284
AoxI GGCC 9 cut(s) 136, 155, 159, 237, 292, 533, 609, 654, 795
ApeKI GCWGC 1 cut(s) 314
ApoI RAATTY 2 cut(s) 482, 769
AspLEI GCGC 2 cut(s) 258, 439
AspS9I GGNCC 4 cut(s) 224, 533, 610, 691
AsuC2I CCSGG 1 cut(s) 119
AsuHPI GGTGA 4 cut(s) 32, 212, 481, 581
AvaI CYCGRG 3 cut(s) 50, 272, 323
AvaII GGWCC 2 cut(s) 224, 691
AxyI CCTNAGG 1 cut(s) 617
BanII GRGCYC 2 cut(s) 126, 279
BbrPI CACGTG 2 cut(s) 430, 688
BbsI GAAGAC 1 cut(s) 36
Bbv12I GWGCWC 2 cut(s) 126, 279
BbvI GCAGC 1 cut(s) 326
BccI CCATC 3 cut(s) 79, 104, 601
BceAI ACGGC 3 cut(s) 205, 430, 520
BciT130I CCWGG 3 cut(s) 221, 658, 712
BciVI GTATCC 1 cut(s) 125
BcnI CCSGG 1 cut(s) 119
BcoDI GTCTC 3 cut(s) 47, 135, 400
BfaI CTAG 1 cut(s) 207
BfoI RGCGCY 2 cut(s) 259, 440
BfuI GTATCC 1 cut(s) 125
BisI GCNGC 7 cut(s) 162, 309, 312, 315, 418, 435, 456
BlsI GCNGC 7 cut(s) 163, 310, 313, 316, 419, 436, 457
Bme1390I CCNGG 4 cut(s) 119, 221, 658, 712
Bme18I GGWCC 2 cut(s) 224, 691
BmeT110I CYCGRG 3 cut(s) 50, 272, 323
BmgBI CACGTC 2 cut(s) 358, 463
BmgT120I GGNCC 4 cut(s) 224, 533, 610, 691
BmiI GGNNCC 6 cut(s) 225, 226, 283, 289, 693, 709
BmrFI CCNGG 4 cut(s) 119, 221, 658, 712
BmsI GCATC 1 cut(s) 25
BpiI GAAGAC 1 cut(s) 36
BplI GAGNNNNNCTC 2 cut(s) 266, 298
BpmI CTGGAG 1 cut(s) 694
BpuEI CTTGAG 1 cut(s) 117
BpuMI CCSGG 1 cut(s) 119
BsaAI YACGTR 2 cut(s) 430, 688
BsaHI GRCGYC 1 cut(s) 500
BsaI GGTCTC 2 cut(s) 47, 135
BsaJI CCNNGG 6 cut(s) 196, 219, 220, 240, 703, 711
BsaWI WCCGGW 2 cut(s) 126, 284
BsaXI ACNNNNNCTCC 6 cut(s) 72, 102, 113, 143, 711, 741
Bsc4I CCNNNNNNNGG 4 cut(s) 324, 433, 618, 704
Bse118I RCCGGY 3 cut(s) 157, 290, 776
Bse1I ACTGG 1 cut(s) 213
Bse21I CCTNAGG 1 cut(s) 617
Bse3DI GCAATG 1 cut(s) 82
BseAI TCCGGA 1 cut(s) 284
BseBI CCWGG 3 cut(s) 221, 658, 712
BseDI CCNNGG 6 cut(s) 196, 219, 220, 240, 703, 711
BseGI GGATG 2 cut(s) 360, 511
BseLI CCNNNNNNNGG 4 cut(s) 324, 433, 618, 704
BseMI GCAATG 1 cut(s) 82
BseMII CTCAG 2 cut(s) 117, 608
BseNI ACTGG 1 cut(s) 213
BseRI GAGGAG 4 cut(s) 164, 173, 593, 602
BseX3I CGGCCG 1 cut(s) 159
BseXI GCAGC 1 cut(s) 326
BseYI CCCAGC 1 cut(s) 439
Bsh1236I CGCG 1 cut(s) 743
Bsh1285I CGRYCG 1 cut(s) 162
BshFI GGCC 9 cut(s) 138, 157, 161, 239, 294, 535, 611, 656, 797
BsiEI CGRYCG 1 cut(s) 162
BsiHKAI GWGCWC 2 cut(s) 126, 279
BsiHKCI CYCGRG 3 cut(s) 50, 272, 323
BsiSI CCGG 6 cut(s) 118, 127, 158, 285, 291, 777
BslFI GGGAC 2 cut(s) 201, 237
BslI CCNNNNNNNGG 4 cut(s) 324, 433, 618, 704
BsmAI GTCTC 3 cut(s) 47, 135, 400
BsmBI CGTCTC 1 cut(s) 400
BsmFI GGGAC 2 cut(s) 201, 237
BsnI GGCC 9 cut(s) 138, 157, 161, 239, 294, 535, 611, 656, 797
Bso31I GGTCTC 2 cut(s) 47, 135
BsoBI CYCGRG 3 cut(s) 50, 272, 323
Bsp1286I GDGCHC 2 cut(s) 126, 279
Bsp13I TCCGGA 1 cut(s) 284
Bsp143I GATC 4 cut(s) 7, 45, 487, 520
Bsp19I CCATGG 1 cut(s) 703
BspANI GGCC 9 cut(s) 138, 157, 161, 239, 294, 535, 611, 656, 797
BspCNI CTCAG 2 cut(s) 116, 609
BspEI TCCGGA 1 cut(s) 284
BspFNI CGCG 1 cut(s) 743
BspLI GGNNCC 6 cut(s) 225, 226, 283, 289, 693, 709
BspPI GGATC 2 cut(s) 15, 40
BspTNI GGTCTC 2 cut(s) 47, 135
BsrDI GCAATG 1 cut(s) 82
BsrFI RCCGGY 3 cut(s) 157, 290, 776
BsrI ACTGG 1 cut(s) 213
BssAI RCCGGY 3 cut(s) 157, 290, 776
BssECI CCNNGG 6 cut(s) 196, 219, 220, 240, 703, 711
BssMI GATC 4 cut(s) 7, 45, 487, 520
BssNI GRCGYC 1 cut(s) 500
BssT1I CCWWGG 2 cut(s) 240, 703
Bst2UI CCWGG 3 cut(s) 221, 658, 712
Bst4CI ACNGT 4 cut(s) 200, 334, 340, 727
BstACI GRCGYC 1 cut(s) 500
BstBAI YACGTR 2 cut(s) 430, 688
BstC8I GCNNGC 1 cut(s) 159
BstDEI CTNAG 2 cut(s) 103, 617
BstDSI CCRYGG 2 cut(s) 196, 703
BstEII GGTNACC 1 cut(s) 200
BstF5I GGATG 2 cut(s) 360, 511
BstFNI CGCG 1 cut(s) 743
BstH2I RGCGCY 2 cut(s) 259, 440
BstHHI GCGC 2 cut(s) 258, 439
BstKTI GATC 4 cut(s) 10, 48, 490, 523
BstMAI GTCTC 3 cut(s) 47, 135, 400
BstMBI GATC 4 cut(s) 7, 45, 487, 520
BstMCI CGRYCG 1 cut(s) 162
BstMWI GCNNNNNNNGC 1 cut(s) 314
BstNI CCWGG 3 cut(s) 221, 658, 712
BstNSI RCATGY 1 cut(s) 250
BstPI GGTNACC 1 cut(s) 200
BstSCI CCNGG 4 cut(s) 117, 219, 656, 710
BstUI CGCG 1 cut(s) 743
BstV1I GCAGC 1 cut(s) 326
BstV2I GAAGAC 1 cut(s) 36
BstXI CCANNNNNNTGG 1 cut(s) 220
BstZI CGGCCG 1 cut(s) 159
Bsu36I CCTNAGG 1 cut(s) 617
BsuI GTATCC 1 cut(s) 125
BsuRI GGCC 9 cut(s) 138, 157, 161, 239, 294, 535, 611, 656, 797
BtgI CCRYGG 2 cut(s) 196, 703
BtrI CACGTC 2 cut(s) 358, 463
BtsCI GGATG 2 cut(s) 360, 511
BtsIMutI CAGTG 1 cut(s) 342
Cac8I GCNNGC 1 cut(s) 159
CfoI GCGC 2 cut(s) 258, 439
Cfr10I RCCGGY 3 cut(s) 157, 290, 776
Cfr13I GGNCC 4 cut(s) 224, 533, 610, 691
CseI GACGC 1 cut(s) 489
CviAII CATG 2 cut(s) 247, 704
DdeI CTNAG 2 cut(s) 103, 617
DpnI GATC 4 cut(s) 9, 47, 489, 522
DpnII GATC 4 cut(s) 7, 45, 487, 520
EaeI YGGCCR 2 cut(s) 155, 159
EagI CGGCCG 1 cut(s) 159
EciI GGCGGA 3 cut(s) 20, 473, 590
Ecl136II GAGCTC 2 cut(s) 124, 277
EclXI CGGCCG 1 cut(s) 159
Eco130I CCWWGG 2 cut(s) 240, 703
Eco147I AGGCCT 1 cut(s) 138
Eco24I GRGCYC 2 cut(s) 126, 279
Eco31I GGTCTC 2 cut(s) 47, 135
Eco47I GGWCC 2 cut(s) 224, 691
Eco52I CGGCCG 1 cut(s) 159
Eco53kI GAGCTC 2 cut(s) 124, 277
Eco72I CACGTG 2 cut(s) 430, 688
Eco81I CCTNAGG 1 cut(s) 617
Eco88I CYCGRG 3 cut(s) 50, 272, 323
Eco91I GGTNACC 1 cut(s) 200
EcoICRI GAGCTC 2 cut(s) 124, 277
EcoO109I RGGNCCY 1 cut(s) 224
EcoO65I GGTNACC 1 cut(s) 200
EcoRI GAATTC 1 cut(s) 769
EcoRII CCWGG 3 cut(s) 219, 656, 710
EcoT14I CCWWGG 2 cut(s) 240, 703
EcoT38I GRGCYC 2 cut(s) 126, 279
ErhI CCWWGG 2 cut(s) 240, 703
Esp3I CGTCTC 1 cut(s) 400
FaeI CATG 2 cut(s) 250, 707
FaiI YATR 2 cut(s) 248, 705
FaqI GGGAC 2 cut(s) 201, 237
FatI CATG 2 cut(s) 246, 703
FauI CCCGC 1 cut(s) 471
Fnu4HI GCNGC 7 cut(s) 162, 309, 312, 315, 418, 435, 456
FokI GGATG 2 cut(s) 347, 518
FriOI GRGCYC 2 cut(s) 126, 279
FseI GGCCGGCC 1 cut(s) 161
Fsp4HI GCNGC 7 cut(s) 162, 309, 312, 315, 418, 435, 456
FspBI CTAG 1 cut(s) 207
GlaI GCGC 2 cut(s) 257, 438
GluI GCNGC 7 cut(s) 162, 309, 312, 315, 418, 435, 456
GsaI CCCAGC 1 cut(s) 443
GsuI CTGGAG 1 cut(s) 694
HaeII RGCGCY 2 cut(s) 259, 440
HaeIII GGCC 9 cut(s) 138, 157, 161, 239, 294, 535, 611, 656, 797
HapII CCGG 6 cut(s) 118, 127, 158, 285, 291, 777
HgaI GACGC 1 cut(s) 489
HhaI GCGC 2 cut(s) 258, 439
Hin1I GRCGYC 1 cut(s) 500
Hin1II CATG 2 cut(s) 250, 707
Hin6I GCGC 2 cut(s) 256, 437
HinP1I GCGC 2 cut(s) 256, 437
HinfI GANTC 4 cut(s) 23, 76, 303, 635
HpaII CCGG 6 cut(s) 118, 127, 158, 285, 291, 777
HphI GGTGA 4 cut(s) 32, 212, 481, 581
Hpy166II GTNNAC 2 cut(s) 427, 691
Hpy188I TCNGA 4 cut(s) 28, 302, 505, 759
Hpy188III TCNNGA 5 cut(s) 11, 285, 518, 604, 649
Hpy8I GTNNAC 2 cut(s) 427, 691
Hpy99I CGWCG 3 cut(s) 191, 302, 505
HpyAV CCTTC 5 cut(s) 8, 77, 625, 646, 655
HpyCH4III ACNGT 4 cut(s) 200, 334, 340, 727
HpyCH4IV ACGT 7 cut(s) 357, 381, 393, 429, 462, 548, 687
HpyCH4V TGCA 2 cut(s) 38, 685
HpyF10VI GCNNNNNNNGC 1 cut(s) 314
HpyF3I CTNAG 2 cut(s) 103, 617
HpySE526I ACGT 7 cut(s) 357, 381, 393, 429, 462, 548, 687
Hsp92I GRCGYC 1 cut(s) 500
Hsp92II CATG 2 cut(s) 250, 707
HspAI GCGC 2 cut(s) 256, 437
KflI GGGWCCC 1 cut(s) 224
Kpn2I TCCGGA 1 cut(s) 284
KroI GCCGGC 1 cut(s) 157
KroNI GCCGGC 1 cut(s) 159
Kzo9I GATC 4 cut(s) 7, 45, 487, 520
LmnI GCTCC 6 cut(s) 69, 121, 129, 177, 287, 713
Lsp1109I GCAGC 1 cut(s) 326
LweI GCATC 1 cut(s) 25
MaeI CTAG 1 cut(s) 207
MaeII ACGT 7 cut(s) 357, 381, 393, 429, 462, 548, 687
MaeIII GTNAC 5 cut(s) 200, 334, 340, 721, 790
MalI GATC 4 cut(s) 9, 47, 489, 522
MboI GATC 4 cut(s) 7, 45, 487, 520
MboII GAAGA 4 cut(s) 41, 637, 640, 757
MhlI GDGCHC 2 cut(s) 126, 279
MluCI AATT 2 cut(s) 482, 769
MlyI GAGTC 2 cut(s) 70, 644
MmeI TCCRAC 4 cut(s) 280, 483, 697, 737
MroI TCCGGA 1 cut(s) 284
MroNI GCCGGC 1 cut(s) 157
MspI CCGG 6 cut(s) 118, 127, 158, 285, 291, 777
MspR9I CCNGG 4 cut(s) 119, 221, 658, 712
MvaI CCWGG 3 cut(s) 221, 658, 712
MvnI CGCG 1 cut(s) 743
MwoI GCNNNNNNNGC 1 cut(s) 314
NaeI GCCGGC 1 cut(s) 159
NciI CCSGG 1 cut(s) 119
NcoI CCATGG 1 cut(s) 703
NdeII GATC 4 cut(s) 7, 45, 487, 520
NgoMIV GCCGGC 1 cut(s) 157
NlaIII CATG 2 cut(s) 250, 707
NlaIV GGNNCC 6 cut(s) 225, 226, 283, 289, 693, 709
NmeAIII GCCGAG 2 cut(s) 215, 258
NmuCI GTSAC 4 cut(s) 200, 334, 340, 790
NspI RCATGY 1 cut(s) 250
PaeR7I CTCGAG 1 cut(s) 272
PasI CCCWGGG 1 cut(s) 220
PceI AGGCCT 1 cut(s) 138
PcsI WCGNNNNNNNCGW 1 cut(s) 330
PdiI GCCGGC 1 cut(s) 159
PfeI GAWTC 2 cut(s) 23, 303
PfoI TCCNGGA 1 cut(s) 117
PkrI GCNGC 7 cut(s) 163, 310, 313, 316, 419, 436, 457
PleI GAGTC 2 cut(s) 70, 643
PmaCI CACGTG 2 cut(s) 430, 688
PmlI CACGTG 2 cut(s) 430, 688
PpsI GAGTC 2 cut(s) 70, 643
Ppu21I YACGTR 2 cut(s) 430, 688
PpuMI RGGWCCY 1 cut(s) 224
Psp124BI GAGCTC 2 cut(s) 126, 279
Psp1406I AACGTT 1 cut(s) 548
Psp5II RGGWCCY 1 cut(s) 224
Psp6I CCWGG 3 cut(s) 219, 656, 710
PspCI CACGTG 2 cut(s) 430, 688
PspEI GGTNACC 1 cut(s) 200
PspFI CCCAGC 1 cut(s) 439
PspGI CCWGG 3 cut(s) 219, 656, 710
PspN4I GGNNCC 6 cut(s) 225, 226, 283, 289, 693, 709
PspPI GGNCC 4 cut(s) 224, 533, 610, 691
PspPPI RGGWCCY 1 cut(s) 224
PspXI VCTCGAGB 1 cut(s) 272
RigI GGCCGGCC 1 cut(s) 161
SacI GAGCTC 2 cut(s) 126, 279
SatI GCNGC 7 cut(s) 162, 309, 312, 315, 418, 435, 456
Sau3AI GATC 4 cut(s) 7, 45, 487, 520
Sau96I GGNCC 4 cut(s) 224, 533, 610, 691
SchI GAGTC 2 cut(s) 70, 644
ScrFI CCNGG 4 cut(s) 119, 221, 658, 712
SduI GDGCHC 2 cut(s) 126, 279
SfaNI GCATC 1 cut(s) 25
Sfr274I CTCGAG 1 cut(s) 272
SinI GGWCC 2 cut(s) 224, 691
SlaI CTCGAG 1 cut(s) 272
SmlI CTYRAG 2 cut(s) 132, 272
SmoI CTYRAG 2 cut(s) 132, 272
Sse9I AATT 2 cut(s) 482, 769
SseBI AGGCCT 1 cut(s) 138
SspMI CTAG 1 cut(s) 207
SstI GAGCTC 2 cut(s) 126, 279
StuI AGGCCT 1 cut(s) 138
StyD4I CCNGG 4 cut(s) 117, 219, 656, 710
StyI CCWWGG 2 cut(s) 240, 703
TaaI ACNGT 4 cut(s) 200, 334, 340, 727
TaiI ACGT 7 cut(s) 360, 384, 396, 432, 465, 551, 690
TaqI TCGA 3 cut(s) 74, 186, 273
TasI AATT 2 cut(s) 482, 769
TauI GCSGC 6 cut(s) 164, 311, 314, 420, 437, 458
TfiI GAWTC 2 cut(s) 23, 303
TscAI CASTG 1 cut(s) 349
TseFI GTSAC 4 cut(s) 200, 334, 340, 790
TseI GCWGC 1 cut(s) 314
Tsp45I GTSAC 4 cut(s) 200, 334, 340, 790
TspGWI ACGGA 2 cut(s) 388, 722
TspRI CASTG 1 cut(s) 349
VpaK11BI GGWCC 2 cut(s) 224, 691
XapI RAATTY 2 cut(s) 482, 769
XceI RCATGY 1 cut(s) 250
XhoI CTCGAG 1 cut(s) 272
XspI CTAG 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.