Rh2CG154000

Protein N-lysine methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0012035
Physical Location & Seq
Forward (+)
13382697 .. 13383512
816 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG154000.1

Sequence Viewer

Length: 816 bp
ATGGCCGCTCTAGAAGAAGACCAAGACGACGTCGTTATAAACCCCGCCACAATGCTCCTCCGCGACGACGACTCCGACTCCGACGAGGTCAAGATGGTGGAGAGCGAACAGCTCCGGCAGCATTCTCTGCCTTCGATCGACTCCACAATCGTCACCAGGCAGCTCCCCTCGCAGGGTCTCTCCTTTCAGCTCTGGCCGGCGGCCACCACGCTCCTCACTCTCCTGGACGACCACCGCCGTGATCCCACCACGAGCCCCCTTGGCCCCACGCTCTCGGCCTTGGAATCCGGCCGGAGACTCAAGATCCTCGAACTAGGTTCTGGAACCGGCCTCGTCGGAATAGCCGCCGCCGCCACGCTCGGCGCGTGCGTCACTGTGACGGACCTCCCTCACGTCGTCGACAACCTTAAGTTCAATGCGGAACTAAACGCCGCCGCTTTGGGAGGGAGCGGCGGGAGTGTGAGAGTGGCGGCGCTGAGGTGGGGAGAGGGGGAGGACGCGGAGGCGATAGGGCGGGAGTTTGATATTGTATTGGCGTCGGATGTAGTGTATCACGACCACCTCTACGAGCCGCTGCTCAAAACGCTGTCGTTTCTGCTGTTGGGGGAGGGCGGCGTGGTGTTTGTGATGGCCCACTTGAGGAGGTGGAAGAAGGAGTCGGCCTTCTTCAAGAGGGCCAGGAAGCTTTTTGAGGTTGAGGTCTTGCACGTGGATCGTCCGTGCCAGGGATCGCGGGTCGGAGTTACTGTTTACCGTTTTACCGGGAAGCCAAGTAAGAAATTGTTGAATGGTGTCTCTGTCAATGGCATTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

271

Amino Acids

29.38

Weight (kDa)

5.41

Isoelectric Point (pI)

39.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_16 PF10294 56 - 227 8.6e-31 Lysine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012035)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50850 AT5G49560
fragaria_vesca FvH4_1g13310
malus_domestica MD02G1147500.v1.1 MD15G1261800.v1.1
prunus_persica Prupe.7G154600_v2.0.a1
pyrus_communis pycom15g22900
rosa_chinensis RchiOBHm_Chr2g0101571
rosa_laevigata RLG00000017091
rosa_multiflora Rmu_co8339275.1_g000001 Rmu_sc0006153.1_g000007 Rmu_sc0019613.1_g000002
rosa_roxburghii Rroxscaffold_2G00141180
rosa_rugosa Rorug02G0097300
rosa_samantha Rh2AG148100 Rh2BG153500 Rh2CG154000 Rh2DG153800
rosa_wichuraiana Rw2G011550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 38
AatII GACGTC 1 cut(s) 33
AccBSI CCGCTC 2 cut(s) 8, 450
AccI GTMKAC 1 cut(s) 399
AccII CGCG 4 cut(s) 63, 365, 500, 733
AclWI GGATC 4 cut(s) 236, 298, 720, 736
AcoI YGGCCR 4 cut(s) 3, 194, 201, 289
AcvI CACGTG 1 cut(s) 709
AcyI GRCGYC 2 cut(s) 30, 536
AfiI CCNNNNNNNGG 4 cut(s) 172, 173, 639, 725
AflII CTTAAG 1 cut(s) 407
AgsI TTSAA 3 cut(s) 415, 670, 787
AjiI CACGTC 1 cut(s) 394
AjnI CCWGG 4 cut(s) 155, 222, 677, 723
AleI CACNNNNGTG 1 cut(s) 237
AluBI AGCT 4 cut(s) 112, 163, 190, 685
AluI AGCT 4 cut(s) 112, 163, 190, 685
Alw26I GTCTC 3 cut(s) 182, 289, 799
AlwI GGATC 4 cut(s) 236, 298, 720, 736
ApeKI GCWGC 3 cut(s) 118, 160, 574
AspLEI GCGC 2 cut(s) 365, 475
AspS9I GGNCC 4 cut(s) 263, 382, 631, 675
AsuC2I CCSGG 1 cut(s) 763
AsuHPI GGTGA 1 cut(s) 145
AvaII GGWCC 1 cut(s) 382
BanII GRGCYC 1 cut(s) 257
BauI CACGAG 1 cut(s) 250
BbrPI CACGTG 1 cut(s) 709
BbsI GAAGAC 1 cut(s) 24
BbvCI CCTCAGC 1 cut(s) 476
BbvI GCAGC 3 cut(s) 130, 172, 561
BccI CCATC 2 cut(s) 88, 622
BceAI ACGGC 1 cut(s) 222
BcgI CGANNNNNNTGC 2 cut(s) 695, 729
BciT130I CCWGG 4 cut(s) 157, 224, 679, 725
BcnI CCSGG 1 cut(s) 763
BcoDI GTCTC 3 cut(s) 182, 289, 799
BfaI CTAG 2 cut(s) 11, 314
BfoI RGCGCY 1 cut(s) 476
BfrI CTTAAG 1 cut(s) 407
BglI GCCNNNNNGGC 1 cut(s) 261
Bme1390I CCNGG 5 cut(s) 157, 224, 679, 725, 763
Bme18I GGWCC 1 cut(s) 382
BmgBI CACGTC 1 cut(s) 394
BmgT120I GGNCC 4 cut(s) 263, 382, 631, 675
BmiI GGNNCC 2 cut(s) 265, 325
BmrFI CCNGG 5 cut(s) 157, 224, 679, 725, 763
BpiI GAAGAC 1 cut(s) 24
Bpu10I CCTNAGC 1 cut(s) 476
BpuEI CTTGAG 2 cut(s) 284, 658
BpuMI CCSGG 1 cut(s) 763
BsaAI YACGTR 1 cut(s) 709
BsaHI GRCGYC 2 cut(s) 30, 536
BsaI GGTCTC 1 cut(s) 182
BsaJI CCNNGG 3 cut(s) 259, 279, 724
BsaXI ACNNNNNCTCC 6 cut(s) 56, 62, 86, 92, 732, 762
Bsc4I CCNNNNNNNGG 4 cut(s) 172, 173, 639, 725
Bse118I RCCGGY 2 cut(s) 196, 326
BseBI CCWGG 4 cut(s) 157, 224, 679, 725
BseDI CCNNGG 3 cut(s) 259, 279, 724
BseGI GGATG 1 cut(s) 547
BseLI CCNNNNNNNGG 4 cut(s) 172, 173, 639, 725
BseMII CTCAG 1 cut(s) 467
BseRI GAGGAG 3 cut(s) 47, 203, 655
BseX3I CGGCCG 1 cut(s) 289
BseXI GCAGC 3 cut(s) 130, 172, 561
Bsh1236I CGCG 4 cut(s) 63, 365, 500, 733
Bsh1285I CGRYCG 2 cut(s) 138, 292
BsiEI CGRYCG 2 cut(s) 138, 292
BsiSI CCGG 6 cut(s) 115, 197, 288, 292, 327, 762
BslI CCNNNNNNNGG 4 cut(s) 172, 173, 639, 725
BsmAI GTCTC 3 cut(s) 182, 289, 799
BsmI GAATGC 2 cut(s) 121, 807
Bso31I GGTCTC 1 cut(s) 182
Bsp1286I GDGCHC 1 cut(s) 257
Bsp143I GATC 5 cut(s) 135, 241, 303, 712, 728
BspCNI CTCAG 1 cut(s) 468
BspFNI CGCG 4 cut(s) 63, 365, 500, 733
BspLI GGNNCC 2 cut(s) 265, 325
BspPI GGATC 4 cut(s) 236, 298, 720, 736
BspTI CTTAAG 1 cut(s) 407
BspTNI GGTCTC 1 cut(s) 182
BsrBI CCGCTC 2 cut(s) 8, 450
BsrFI RCCGGY 2 cut(s) 196, 326
BssAI RCCGGY 2 cut(s) 196, 326
BssECI CCNNGG 3 cut(s) 259, 279, 724
BssMI GATC 5 cut(s) 135, 241, 303, 712, 728
BssNI GRCGYC 2 cut(s) 30, 536
BssSI CACGAG 1 cut(s) 250
BssT1I CCWWGG 2 cut(s) 259, 279
Bst2BI CACGAG 1 cut(s) 250
Bst2UI CCWGG 4 cut(s) 157, 224, 679, 725
Bst4CI ACNGT 3 cut(s) 376, 748, 755
BstACI GRCGYC 2 cut(s) 30, 536
BstAFI CTTAAG 1 cut(s) 407
BstAPI GCANNNNNTGC 1 cut(s) 127
BstBAI YACGTR 1 cut(s) 709
BstC8I GCNNGC 2 cut(s) 198, 367
BstDEI CTNAG 1 cut(s) 476
BstF5I GGATG 1 cut(s) 547
BstFNI CGCG 4 cut(s) 63, 365, 500, 733
BstH2I RGCGCY 1 cut(s) 476
BstHHI GCGC 2 cut(s) 365, 475
BstKTI GATC 5 cut(s) 138, 244, 306, 715, 731
BstMAI GTCTC 3 cut(s) 182, 289, 799
BstMBI GATC 5 cut(s) 135, 241, 303, 712, 728
BstMCI CGRYCG 2 cut(s) 138, 292
BstMWI GCNNNNNNNGC 6 cut(s) 118, 127, 169, 261, 350, 583
BstNI CCWGG 4 cut(s) 157, 224, 679, 725
BstSCI CCNGG 5 cut(s) 155, 222, 677, 723, 761
BstUI CGCG 4 cut(s) 63, 365, 500, 733
BstV1I GCAGC 3 cut(s) 130, 172, 561
BstV2I GAAGAC 1 cut(s) 24
BstX2I RGATCY 1 cut(s) 303
BstYI RGATCY 1 cut(s) 303
BstZI CGGCCG 1 cut(s) 289
BtrI CACGTC 1 cut(s) 394
BtsCI GGATG 1 cut(s) 547
BtsIMutI CAGTG 1 cut(s) 372
Cac8I GCNNGC 2 cut(s) 198, 367
CfoI GCGC 2 cut(s) 365, 475
Cfr10I RCCGGY 2 cut(s) 196, 326
Cfr13I GGNCC 4 cut(s) 263, 382, 631, 675
CseI GACGC 3 cut(s) 358, 506, 525
DdeI CTNAG 1 cut(s) 476
DpnI GATC 5 cut(s) 137, 243, 305, 714, 730
DpnII GATC 5 cut(s) 135, 241, 303, 712, 728
EaeI YGGCCR 4 cut(s) 3, 194, 201, 289
EagI CGGCCG 1 cut(s) 289
EclXI CGGCCG 1 cut(s) 289
Eco130I CCWWGG 2 cut(s) 259, 279
Eco24I GRGCYC 1 cut(s) 257
Eco31I GGTCTC 1 cut(s) 182
Eco47I GGWCC 1 cut(s) 382
Eco52I CGGCCG 1 cut(s) 289
Eco72I CACGTG 1 cut(s) 709
EcoRII CCWGG 4 cut(s) 155, 222, 677, 723
EcoT14I CCWWGG 2 cut(s) 259, 279
EcoT38I GRGCYC 1 cut(s) 257
ErhI CCWWGG 2 cut(s) 259, 279
FaiI YATR 1 cut(s) 38
FauI CCCGC 4 cut(s) 52, 446, 507, 726
FblI GTMKAC 1 cut(s) 399
FokI GGATG 1 cut(s) 554
FriOI GRGCYC 1 cut(s) 257
FspBI CTAG 2 cut(s) 11, 314
GlaI GCGC 2 cut(s) 364, 474
HaeII RGCGCY 1 cut(s) 476
HapII CCGG 6 cut(s) 115, 197, 288, 292, 327, 762
HgaI GACGC 3 cut(s) 358, 506, 525
HhaI GCGC 2 cut(s) 365, 475
Hin1I GRCGYC 2 cut(s) 30, 536
Hin6I GCGC 2 cut(s) 363, 473
HinP1I GCGC 2 cut(s) 363, 473
HincII GTYRAC 1 cut(s) 400
HindII GTYRAC 1 cut(s) 400
HindIII AAGCTT 1 cut(s) 683
HinfI GANTC 6 cut(s) 71, 77, 140, 284, 297, 656
HpaII CCGG 6 cut(s) 115, 197, 288, 292, 327, 762
HphI GGTGA 1 cut(s) 145
Hpy166II GTNNAC 2 cut(s) 400, 751
Hpy188I TCNGA 5 cut(s) 76, 82, 338, 541, 740
Hpy188III TCNNGA 6 cut(s) 11, 91, 301, 321, 554, 670
Hpy8I GTNNAC 2 cut(s) 400, 751
Hpy99I CGWCG 9 cut(s) 32, 35, 68, 71, 86, 338, 398, 401, 541
HpyAV CCTTC 3 cut(s) 141, 646, 673
HpyCH4III ACNGT 3 cut(s) 376, 748, 755
HpyCH4IV ACGT 3 cut(s) 30, 393, 708
HpyCH4V TGCA 1 cut(s) 706
HpyF10VI GCNNNNNNNGC 6 cut(s) 118, 127, 169, 261, 350, 583
HpyF3I CTNAG 1 cut(s) 476
HpySE526I ACGT 3 cut(s) 30, 393, 708
Hsp92I GRCGYC 2 cut(s) 30, 536
HspAI GCGC 2 cut(s) 363, 473
KroI GCCGGC 1 cut(s) 196
KroNI GCCGGC 1 cut(s) 198
Kzo9I GATC 5 cut(s) 135, 241, 303, 712, 728
LmnI GCTCC 5 cut(s) 60, 117, 168, 216, 447
Lsp1109I GCAGC 3 cut(s) 130, 172, 561
MaeI CTAG 2 cut(s) 11, 314
MaeII ACGT 3 cut(s) 30, 393, 708
MaeIII GTNAC 4 cut(s) 151, 370, 376, 742
MalI GATC 5 cut(s) 137, 243, 305, 714, 730
MbiI CCGCTC 2 cut(s) 8, 450
MboI GATC 5 cut(s) 135, 241, 303, 712, 728
MboII GAAGA 4 cut(s) 26, 29, 658, 661
MflI RGATCY 1 cut(s) 303
MhlI GDGCHC 1 cut(s) 257
MluCI AATT 1 cut(s) 779
MlyI GAGTC 5 cut(s) 65, 71, 134, 291, 665
MmeI TCCRAC 5 cut(s) 99, 105, 316, 519, 718
MroNI GCCGGC 1 cut(s) 196
MseI TTAA 1 cut(s) 408
MslI CAYNNNNRTG 1 cut(s) 237
MspA1I CMGCKG 1 cut(s) 574
MspCI CTTAAG 1 cut(s) 407
MspI CCGG 6 cut(s) 115, 197, 288, 292, 327, 762
MspR9I CCNGG 5 cut(s) 157, 224, 679, 725, 763
Mva1269I GAATGC 2 cut(s) 121, 807
MvaI CCWGG 4 cut(s) 157, 224, 679, 725
MvnI CGCG 4 cut(s) 63, 365, 500, 733
MwoI GCNNNNNNNGC 6 cut(s) 118, 127, 169, 261, 350, 583
NaeI GCCGGC 1 cut(s) 198
NciI CCSGG 1 cut(s) 763
NdeII GATC 5 cut(s) 135, 241, 303, 712, 728
NgoMIV GCCGGC 1 cut(s) 196
NlaIV GGNNCC 2 cut(s) 265, 325
NmeAIII GCCGAG 2 cut(s) 254, 339
NmuCI GTSAC 3 cut(s) 151, 370, 376
OliI CACNNNNGTG 1 cut(s) 237
PcsI WCGNNNNNNNCGW 3 cut(s) 72, 362, 366
PctI GAATGC 2 cut(s) 121, 807
PdiI GCCGGC 1 cut(s) 198
PfeI GAWTC 1 cut(s) 284
PflFI GACNNNGTC 2 cut(s) 29, 86
PfoI TCCNGGA 1 cut(s) 222
Ple19I CGATCG 1 cut(s) 138
PleI GAGTC 5 cut(s) 65, 71, 134, 291, 664
PmaCI CACGTG 1 cut(s) 709
PmlI CACGTG 1 cut(s) 709
PpsI GAGTC 5 cut(s) 65, 71, 134, 291, 664
Ppu21I YACGTR 1 cut(s) 709
PsiI TTATAA 1 cut(s) 38
Psp6I CCWGG 4 cut(s) 155, 222, 677, 723
PspCI CACGTG 1 cut(s) 709
PspGI CCWGG 4 cut(s) 155, 222, 677, 723
PspN4I GGNNCC 2 cut(s) 265, 325
PspPI GGNCC 4 cut(s) 263, 382, 631, 675
PsuI RGATCY 1 cut(s) 303
PsyI GACNNNGTC 2 cut(s) 29, 86
PvuI CGATCG 1 cut(s) 138
RseI CAYNNNNRTG 1 cut(s) 237
SalI GTCGAC 1 cut(s) 398
SaqAI TTAA 1 cut(s) 408
Sau3AI GATC 5 cut(s) 135, 241, 303, 712, 728
Sau96I GGNCC 4 cut(s) 263, 382, 631, 675
SchI GAGTC 5 cut(s) 65, 71, 134, 291, 665
ScrFI CCNGG 5 cut(s) 157, 224, 679, 725, 763
SduI GDGCHC 1 cut(s) 257
SinI GGWCC 1 cut(s) 382
SmiMI CAYNNNNRTG 1 cut(s) 237
SmlI CTYRAG 3 cut(s) 299, 407, 637
SmoI CTYRAG 3 cut(s) 299, 407, 637
Sse9I AATT 1 cut(s) 779
SspMI CTAG 2 cut(s) 11, 314
StyD4I CCNGG 5 cut(s) 155, 222, 677, 723, 761
StyI CCWWGG 2 cut(s) 259, 279
TaaI ACNGT 3 cut(s) 376, 748, 755
TaiI ACGT 3 cut(s) 33, 396, 711
TaqI TCGA 4 cut(s) 134, 138, 309, 399
TasI AATT 1 cut(s) 779
TfiI GAWTC 1 cut(s) 284
Tru1I TTAA 1 cut(s) 408
Tru9I TTAA 1 cut(s) 408
TscAI CASTG 1 cut(s) 379
TseFI GTSAC 3 cut(s) 151, 370, 376
TseI GCWGC 3 cut(s) 118, 160, 574
Tsp45I GTSAC 3 cut(s) 151, 370, 376
TspGWI ACGGA 2 cut(s) 395, 708
TspRI CASTG 1 cut(s) 379
Tth111I GACNNNGTC 2 cut(s) 29, 86
Vha464I CTTAAG 1 cut(s) 407
VpaK11BI GGWCC 1 cut(s) 382
XbaI TCTAGA 1 cut(s) 10
XmiI GTMKAC 1 cut(s) 399
XspI CTAG 2 cut(s) 11, 314
ZraI GACGTC 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.