AT3G51000

epoxide hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004189
Physical Location & Seq
Reverse (-)
18945025 .. 18946621
1597 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G51000.1

Sequence Viewer

Length: 972 bp
ATGACGAGCTCTGTTAGAGAGAAGAAGATCAAGACCAACGGGATTTGGTTAAACGTTGCTGAGAAAGGAGACGAAGAAGGGCCTTTGGTTCTGTTACTCCATGGTTTCCCTGAGACATGGTACTCTTGGCGTCACCAGATCGATTTCTTGTCTAGCCATGGTTACCACGTGGTCGCTCCAGATCTCAGAGGCTACGGTGACTCCGATTCTCTACCGAGCCACGAGTCTTACACTGTGAGTCACCTAGTCGCTGATGTCATCGGTTTACTCGATCACTACGGCACTACTCAGGCTTTTGTGGCTGGGCATGACTGGGGAGCAATCATAGGTTGGTGTCTATGCTTGTTTAGACCAGACCGAGTCAAAGGTTTCATAAGTCTCTCTGTTCCATATTTCCCAAGAGATCCAAAACTCAAACCTTCAGATTTTTTCAAAATTTTTGGAGATGGTTTATACATCACTCAGTTTCAGAAACCCGGAAGAGCTGAGGCTGCATTTGCCAAGCATGATTGTTTATCGGTAATGAAGAAGTTCTTGCTAATAACAAGAACAGACTACTTAGTGGCTCCTCCTGACACAGAGATCATAGATCATCTTGAGATACCGTCGACGATTCCAGATTGGATAACAGAAGAAGAGATTCAAGTGTATGCGGAAAAGTTTCAAAGATCTGGATTCACTGGCCCTTTGAATTACTACAGATCCATGGATATGAATTGGGAGATATTGGCGCCGTGGCAAGACTCCAAAATCGTCGTTCCTACTAAGTTTATAGCGGGAGACAAAGATATCGGATATGAAGGACCTAATGGAACAATGGAGTATGTGAAAGGAGAGGTGTTCAAGATTGTTGTGCCTAATCTTGAGATAGTTGTTATTGAAGGTGGTCATCATTTTATCCAGCAGGAGAAGTCTGAACAAGTCTCTCAAGAGATTCTCTCTTTCTTAAACAAGTTAAGCAAAACAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

323

Amino Acids

36.7

Weight (kDa)

5.39

Isoelectric Point (pI)

30.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 28 - 176 2e-27 alpha/beta hydrolase fold
Hydrolase_4 PF12146 29 - 142 7e-13 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 30 - 309 1.2e-19 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 730
AccI GTMKAC 1 cut(s) 608
AciI CCGC 2 cut(s) 653, 776
AclI AACGTT 1 cut(s) 54
AclWI GGATC 2 cut(s) 398, 696
AcsI RAATTY 1 cut(s) 435
AcuI CTGAAG 1 cut(s) 405
AcvI CACGTG 1 cut(s) 169
AcyI GRCGYC 2 cut(s) 130, 731
AfaI GTAC 1 cut(s) 122
AgsI TTSAA 6 cut(s) 433, 644, 665, 691, 844, 881
AluBI AGCT 2 cut(s) 9, 485
AluI AGCT 2 cut(s) 9, 485
Alw21I GWGCWC 1 cut(s) 11
Alw26I GTCTC 5 cut(s) 63, 107, 383, 774, 928
AlwI GGATC 2 cut(s) 398, 696
AoxI GGCC 2 cut(s) 80, 682
ApeKI GCWGC 1 cut(s) 491
ApoI RAATTY 1 cut(s) 435
Asp700I GAANNNNTTC 3 cut(s) 530, 639, 660
AspLEI GCGC 1 cut(s) 733
AspS9I GGNCC 3 cut(s) 80, 683, 803
AsuC2I CCSGG 1 cut(s) 477
AsuHPI GGTGA 3 cut(s) 125, 209, 233
AvaII GGWCC 1 cut(s) 803
BanI GGYRCC 1 cut(s) 730
BanII GRGCYC 1 cut(s) 11
BauI CACGAG 1 cut(s) 221
BbrPI CACGTG 1 cut(s) 169
Bbv12I GWGCWC 1 cut(s) 11
BbvCI CCTCAGC 1 cut(s) 486
BbvI GCAGC 1 cut(s) 478
BccI CCATC 1 cut(s) 440
BceAI ACGGC 2 cut(s) 295, 718
BcnI CCSGG 1 cut(s) 477
BcoDI GTCTC 5 cut(s) 63, 107, 383, 774, 928
BfaI CTAG 2 cut(s) 153, 245
BfmI CTRYAG 1 cut(s) 697
BfoI RGCGCY 1 cut(s) 734
BglII AGATCT 2 cut(s) 181, 668
BisI GCNGC 1 cut(s) 492
BlsI GCNGC 1 cut(s) 493
Bme1390I CCNGG 1 cut(s) 477
Bme18I GGWCC 1 cut(s) 803
BmgT120I GGNCC 3 cut(s) 80, 683, 803
BmiI GGNNCC 2 cut(s) 567, 732
BmrFI CCNGG 1 cut(s) 477
BmrI ACTGGG 1 cut(s) 322
BmuI ACTGGG 1 cut(s) 322
BplI GAGNNNNNCTC 2 cut(s) 923, 955
BpmI CTGGAG 1 cut(s) 162
Bpu10I CCTNAGC 1 cut(s) 486
BpuEI CTTGAG 3 cut(s) 617, 884, 912
BpuMI CCSGG 1 cut(s) 477
Bsa29I ATCGAT 1 cut(s) 141
BsaAI YACGTR 1 cut(s) 169
BsaHI GRCGYC 2 cut(s) 130, 731
BsaJI CCNNGG 4 cut(s) 100, 157, 705, 734
BsaXI ACNNNNNCTCC 2 cut(s) 185, 215
Bse1I ACTGG 2 cut(s) 317, 685
BseCI ATCGAT 1 cut(s) 141
BseDI CCNNGG 4 cut(s) 100, 157, 705, 734
BseMII CTCAG 6 cut(s) 51, 102, 199, 302, 476, 477
BseNI ACTGG 2 cut(s) 317, 685
BseRI GAGGAG 1 cut(s) 558
BseXI GCAGC 1 cut(s) 478
BseYI CCCAGC 1 cut(s) 302
BshFI GGCC 2 cut(s) 82, 684
BshNI GGYRCC 1 cut(s) 730
BshVI ATCGAT 1 cut(s) 141
BsiHKAI GWGCWC 1 cut(s) 11
BsiSI CCGG 1 cut(s) 477
BsmAI GTCTC 5 cut(s) 63, 107, 383, 774, 928
BsmBI CGTCTC 1 cut(s) 63
BsnI GGCC 2 cut(s) 82, 684
Bsp1286I GDGCHC 1 cut(s) 11
Bsp143I GATC 9 cut(s) 27, 138, 181, 271, 403, 582, 589, 668, 701
Bsp19I CCATGG 3 cut(s) 100, 157, 705
BspACI CCGC 2 cut(s) 653, 776
BspANI GGCC 2 cut(s) 82, 684
BspCNI CTCAG 6 cut(s) 52, 103, 198, 301, 475, 478
BspDI ATCGAT 1 cut(s) 141
BspLI GGNNCC 2 cut(s) 567, 732
BspPI GGATC 2 cut(s) 398, 696
BspQI GCTCTTC 1 cut(s) 475
BspT107I GGYRCC 1 cut(s) 730
BsrI ACTGG 2 cut(s) 317, 685
BssECI CCNNGG 4 cut(s) 100, 157, 705, 734
BssMI GATC 9 cut(s) 27, 138, 181, 271, 403, 582, 589, 668, 701
BssNI GRCGYC 2 cut(s) 130, 731
BssSI CACGAG 1 cut(s) 221
BssT1I CCWWGG 3 cut(s) 100, 157, 705
Bst2BI CACGAG 1 cut(s) 221
Bst4CI ACNGT 3 cut(s) 197, 235, 606
Bst6I CTCTTC 2 cut(s) 475, 630
BstACI GRCGYC 2 cut(s) 130, 731
BstBAI YACGTR 1 cut(s) 169
BstDEI CTNAG 8 cut(s) 60, 111, 185, 288, 462, 486, 559, 765
BstDSI CCRYGG 4 cut(s) 100, 157, 705, 734
BstEII GGTNACC 1 cut(s) 161
BstH2I RGCGCY 1 cut(s) 734
BstHHI GCGC 1 cut(s) 733
BstKTI GATC 9 cut(s) 30, 141, 184, 274, 406, 585, 592, 671, 704
BstMAI GTCTC 5 cut(s) 63, 107, 383, 774, 928
BstMBI GATC 9 cut(s) 27, 138, 181, 271, 403, 582, 589, 668, 701
BstMWI GCNNNNNNNGC 3 cut(s) 299, 491, 497
BstPI GGTNACC 1 cut(s) 161
BstSCI CCNGG 1 cut(s) 475
BstSFI CTRYAG 1 cut(s) 697
BstV1I GCAGC 1 cut(s) 478
BstX2I RGATCY 4 cut(s) 181, 403, 668, 701
BstYI RGATCY 4 cut(s) 181, 403, 668, 701
Bsu15I ATCGAT 1 cut(s) 141
BsuRI GGCC 2 cut(s) 82, 684
BsuTUI ATCGAT 1 cut(s) 141
BtgI CCRYGG 4 cut(s) 100, 157, 705, 734
BtsIMutI CAGTG 2 cut(s) 231, 678
CfoI GCGC 1 cut(s) 733
Cfr13I GGNCC 3 cut(s) 80, 683, 803
ClaI ATCGAT 1 cut(s) 141
CseI GACGC 1 cut(s) 119
Csp6I GTAC 1 cut(s) 121
CviAII CATG 6 cut(s) 101, 117, 158, 308, 506, 706
CviQI GTAC 1 cut(s) 121
DdeI CTNAG 8 cut(s) 60, 111, 185, 288, 462, 486, 559, 765
DinI GGCGCC 1 cut(s) 732
DpnI GATC 9 cut(s) 29, 140, 183, 273, 405, 584, 591, 670, 703
DpnII GATC 9 cut(s) 27, 138, 181, 271, 403, 582, 589, 668, 701
Eam1104I CTCTTC 2 cut(s) 475, 630
EarI CTCTTC 2 cut(s) 475, 630
Ecl136II GAGCTC 1 cut(s) 9
Eco130I CCWWGG 3 cut(s) 100, 157, 705
Eco24I GRGCYC 1 cut(s) 11
Eco32I GATATC 1 cut(s) 790
Eco47I GGWCC 1 cut(s) 803
Eco53kI GAGCTC 1 cut(s) 9
Eco57I CTGAAG 1 cut(s) 405
Eco72I CACGTG 1 cut(s) 169
Eco91I GGTNACC 1 cut(s) 161
EcoICRI GAGCTC 1 cut(s) 9
EcoO109I RGGNCCY 2 cut(s) 80, 803
EcoO65I GGTNACC 1 cut(s) 161
EcoRV GATATC 1 cut(s) 790
EcoT14I CCWWGG 3 cut(s) 100, 157, 705
EcoT38I GRGCYC 1 cut(s) 11
EgeI GGCGCC 1 cut(s) 732
EheI GGCGCC 1 cut(s) 732
ErhI CCWWGG 3 cut(s) 100, 157, 705
Esp3I CGTCTC 1 cut(s) 63
FaeI CATG 6 cut(s) 104, 120, 161, 311, 509, 709
FalI AAGNNNNNCTT 2 cut(s) 518, 550
FatI CATG 6 cut(s) 100, 116, 157, 307, 505, 705
FauI CCCGC 1 cut(s) 769
FblI GTMKAC 1 cut(s) 608
Fnu4HI GCNGC 1 cut(s) 492
FriOI GRGCYC 1 cut(s) 11
Fsp4HI GCNGC 1 cut(s) 492
FspBI CTAG 2 cut(s) 153, 245
GlaI GCGC 1 cut(s) 732
GluI GCNGC 1 cut(s) 492
GsaI CCCAGC 1 cut(s) 306
GsuI CTGGAG 1 cut(s) 162
HaeII RGCGCY 1 cut(s) 734
HaeIII GGCC 2 cut(s) 82, 684
HapII CCGG 1 cut(s) 477
HgaI GACGC 1 cut(s) 119
HhaI GCGC 1 cut(s) 733
Hin1I GRCGYC 2 cut(s) 130, 731
Hin1II CATG 6 cut(s) 104, 120, 161, 311, 509, 709
Hin6I GCGC 1 cut(s) 731
HinP1I GCGC 1 cut(s) 731
HincII GTYRAC 1 cut(s) 609
HindII GTYRAC 1 cut(s) 609
HpaII CCGG 1 cut(s) 477
HphI GGTGA 3 cut(s) 125, 209, 233
Hpy166II GTNNAC 2 cut(s) 266, 609
Hpy188I TCNGA 6 cut(s) 188, 205, 424, 471, 794, 916
Hpy188III TCNNGA 9 cut(s) 31, 179, 572, 596, 617, 672, 844, 863, 929
Hpy8I GTNNAC 2 cut(s) 266, 609
Hpy99I CGWCG 3 cut(s) 610, 613, 758
HpyAV CCTTC 4 cut(s) 71, 429, 794, 875
HpyCH4III ACNGT 3 cut(s) 197, 235, 606
HpyCH4IV ACGT 2 cut(s) 54, 168
HpyCH4V TGCA 1 cut(s) 494
HpyF10VI GCNNNNNNNGC 3 cut(s) 299, 491, 497
HpyF3I CTNAG 8 cut(s) 60, 111, 185, 288, 462, 486, 559, 765
HpySE526I ACGT 2 cut(s) 54, 168
Hsp92I GRCGYC 2 cut(s) 130, 731
Hsp92II CATG 6 cut(s) 104, 120, 161, 311, 509, 709
HspAI GCGC 1 cut(s) 731
KasI GGCGCC 1 cut(s) 730
Kzo9I GATC 9 cut(s) 27, 138, 181, 271, 403, 582, 589, 668, 701
LguI GCTCTTC 1 cut(s) 475
LmnI GCTCC 3 cut(s) 181, 317, 571
Lsp1109I GCAGC 1 cut(s) 478
MaeI CTAG 2 cut(s) 153, 245
MaeII ACGT 2 cut(s) 54, 168
MaeIII GTNAC 5 cut(s) 93, 131, 161, 197, 239
MalI GATC 9 cut(s) 29, 140, 183, 273, 405, 584, 591, 670, 703
MboI GATC 9 cut(s) 27, 138, 181, 271, 403, 582, 589, 668, 701
MboII GAAGA 7 cut(s) 34, 37, 86, 492, 538, 644, 647
MflI RGATCY 4 cut(s) 181, 403, 668, 701
MhlI GDGCHC 1 cut(s) 11
MluCI AATT 3 cut(s) 435, 691, 715
Mly113I GGCGCC 1 cut(s) 731
MlyI GAGTC 5 cut(s) 194, 233, 247, 369, 737
MnlI CCTC 4 cut(s) 182, 481, 579, 829
MroXI GAANNNNTTC 3 cut(s) 530, 639, 660
MseI TTAA 3 cut(s) 50, 947, 956
MslI CAYNNNNRTG 1 cut(s) 710
MspI CCGG 1 cut(s) 477
MspR9I CCNGG 1 cut(s) 477
MwoI GCNNNNNNNGC 3 cut(s) 299, 491, 497
NarI GGCGCC 1 cut(s) 731
NciI CCSGG 1 cut(s) 477
NcoI CCATGG 3 cut(s) 100, 157, 705
NdeII GATC 9 cut(s) 27, 138, 181, 271, 403, 582, 589, 668, 701
NlaIII CATG 6 cut(s) 104, 120, 161, 311, 509, 709
NlaIV GGNNCC 2 cut(s) 567, 732
NmuCI GTSAC 3 cut(s) 131, 197, 239
PciSI GCTCTTC 1 cut(s) 475
PcsI WCGNNNNNNNCGW 2 cut(s) 201, 267
PdmI GAANNNNTTC 3 cut(s) 530, 639, 660
PfeI GAWTC 5 cut(s) 206, 613, 640, 675, 934
PflFI GACNNNGTC 1 cut(s) 359
PkrI GCNGC 1 cut(s) 493
PleI GAGTC 5 cut(s) 194, 232, 246, 368, 737
PluTI GGCGCC 1 cut(s) 734
PmaCI CACGTG 1 cut(s) 169
PmlI CACGTG 1 cut(s) 169
PpsI GAGTC 5 cut(s) 194, 232, 246, 368, 737
Ppu21I YACGTR 1 cut(s) 169
PpuMI RGGWCCY 1 cut(s) 803
Psp124BI GAGCTC 1 cut(s) 11
Psp1406I AACGTT 1 cut(s) 54
Psp5II RGGWCCY 1 cut(s) 803
PspCI CACGTG 1 cut(s) 169
PspEI GGTNACC 1 cut(s) 161
PspFI CCCAGC 1 cut(s) 302
PspN4I GGNNCC 2 cut(s) 567, 732
PspPI GGNCC 3 cut(s) 80, 683, 803
PspPPI RGGWCCY 1 cut(s) 803
PsuI RGATCY 4 cut(s) 181, 403, 668, 701
PsyI GACNNNGTC 1 cut(s) 359
RsaI GTAC 1 cut(s) 122
RsaNI GTAC 1 cut(s) 121
RseI CAYNNNNRTG 1 cut(s) 710
SacI GAGCTC 1 cut(s) 11
SalI GTCGAC 1 cut(s) 607
SapI GCTCTTC 1 cut(s) 475
SaqAI TTAA 3 cut(s) 50, 947, 956
SatI GCNGC 1 cut(s) 492
Sau3AI GATC 9 cut(s) 27, 138, 181, 271, 403, 582, 589, 668, 701
Sau96I GGNCC 3 cut(s) 80, 683, 803
SchI GAGTC 5 cut(s) 194, 233, 247, 369, 737
ScrFI CCNGG 1 cut(s) 477
SduI GDGCHC 1 cut(s) 11
SfcI CTRYAG 1 cut(s) 697
SfoI GGCGCC 1 cut(s) 732
SgrDI CGTCGACG 1 cut(s) 607
SinI GGWCC 1 cut(s) 803
SmiMI CAYNNNNRTG 1 cut(s) 710
SmlI CTYRAG 3 cut(s) 596, 863, 927
SmoI CTYRAG 3 cut(s) 596, 863, 927
Sse9I AATT 3 cut(s) 435, 691, 715
SsiI CCGC 2 cut(s) 653, 776
SspDI GGCGCC 1 cut(s) 730
SspMI CTAG 2 cut(s) 153, 245
SstI GAGCTC 1 cut(s) 11
StyD4I CCNGG 1 cut(s) 475
StyI CCWWGG 3 cut(s) 100, 157, 705
TaaI ACNGT 3 cut(s) 197, 235, 606
TaiI ACGT 2 cut(s) 57, 171
TaqI TCGA 3 cut(s) 141, 270, 608
TaqII GACCGA 1 cut(s) 372
TasI AATT 3 cut(s) 435, 691, 715
TfiI GAWTC 5 cut(s) 206, 613, 640, 675, 934
Tru1I TTAA 3 cut(s) 50, 947, 956
Tru9I TTAA 3 cut(s) 50, 947, 956
TscAI CASTG 2 cut(s) 238, 685
TseFI GTSAC 3 cut(s) 131, 197, 239
TseI GCWGC 1 cut(s) 491
Tsp45I GTSAC 3 cut(s) 131, 197, 239
TspDTI ATGAA 4 cut(s) 361, 539, 728, 813
TspRI CASTG 2 cut(s) 238, 685
Tth111I GACNNNGTC 1 cut(s) 359
VpaK11BI GGWCC 1 cut(s) 803
XapI RAATTY 1 cut(s) 435
XmiI GTMKAC 1 cut(s) 608
XmnI GAANNNNTTC 3 cut(s) 530, 639, 660
XspI CTAG 2 cut(s) 153, 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.