AT3G53620

Inorganic pyrophosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0007013
Physical Location & Seq
Forward (+)
19880504 .. 19883743
3240 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G53620.1

Sequence Viewer

Length: 651 bp
ATGGCGCCACCGATTGAGGTTTCTACCAAAAGCTACGTTGAGAAACATGTTTCACTTCCTACTCTTAATGAGAGGATACTTTCGTCCATGAGTCACAGATCAGTAGCTGCACACCCATGGCATGATCTCGAGATAGGACCTGAAGCCCCAATTATCTTCAATTGTGTGGTTGAGATAGGAAAAGGGAGCAAGGTGAAATATGAACTCGACAAAACTACGGGTCTCATTAAGGTCGACCGTATTCTTTACTCATCTGTCGTATACCCACACAACTATGGGTTCATTCCGCGTACCCTTTGTGAGGACAGTGACCCTATTGATGTTCTTGTCATTATGCAGGAACCGGTGATCCCAGGATGCTTTCTTCGGGCCAAAGCTATTGGTCTGATGCCAATGATTGATCAGGGTGAGAAAGACGACAAGATCATTGCTGTCTGCGCTGACGATCCAGAGTATCGCCATTACAACGACATCAGTGAGCTTCCGCCTCATCGTATGGCTGAGATCCGCCGTTTCTTTGAAGACTATAAGAAAAACGAGAACAAGGAAGTAGCCGTTAACGACTTCCTTCCGGCAACTGCAGCCTACGACGCAGTTCAGCATTCCATGGATCTCTATGCAGACTACGTCGTGGAGAACCTAAGACGTTGA

Protein Analysis

216

Amino Acids

24.58

Weight (kDa)

5.26

Isoelectric Point (pI)

45.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyrophosphatase PF00719 55 - 206 8.3e-58 Inorganic pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 2 cut(s) 234, 261
AccII CGCG 1 cut(s) 289
AciI CCGC 3 cut(s) 287, 485, 508
AclWI GGATC 4 cut(s) 343, 440, 499, 618
AcuI CTGAAG 1 cut(s) 162
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 292
AfiI CCNNNNNNNGG 1 cut(s) 301
AflIII ACRYGT 1 cut(s) 46
AgeI ACCGGT 1 cut(s) 343
AgsI TTSAA 2 cut(s) 160, 521
AjnI CCWGG 1 cut(s) 352
AloI GAACNNNNNNTCC 2 cut(s) 333, 365
AluBI AGCT 4 cut(s) 33, 107, 377, 481
AluI AGCT 4 cut(s) 33, 107, 377, 481
Alw26I GTCTC 1 cut(s) 227
AlwI GGATC 4 cut(s) 343, 440, 499, 618
AlwNI CAGNNNCTG 1 cut(s) 107
Ama87I CYCGRG 1 cut(s) 128
AoxI GGCC 1 cut(s) 369
ApeKI GCWGC 2 cut(s) 107, 581
AsiGI ACCGGT 1 cut(s) 343
AspLEI GCGC 2 cut(s) 7, 440
AspS9I GGNCC 2 cut(s) 137, 369
AsuHPI GGTGA 3 cut(s) 205, 358, 419
AvaI CYCGRG 1 cut(s) 128
AvaII GGWCC 1 cut(s) 137
BaeI ACNNNNGTAYC 2 cut(s) 68, 101
BanI GGYRCC 1 cut(s) 4
BbsI GAAGAC 1 cut(s) 528
BbvI GCAGC 2 cut(s) 94, 593
BceAI ACGGC 2 cut(s) 495, 539
BciT130I CCWGG 1 cut(s) 354
BciVI GTATCC 1 cut(s) 69
BclI TGATCA 1 cut(s) 400
BcoDI GTCTC 1 cut(s) 227
BfmI CTRYAG 1 cut(s) 579
BfoI RGCGCY 1 cut(s) 8
BfuI GTATCC 1 cut(s) 69
BisI GCNGC 2 cut(s) 108, 582
BlsI GCNGC 2 cut(s) 109, 583
Bme1390I CCNGG 1 cut(s) 354
Bme18I GGWCC 1 cut(s) 137
BmeT110I CYCGRG 1 cut(s) 128
BmgT120I GGNCC 2 cut(s) 137, 369
BmiI GGNNCC 2 cut(s) 6, 342
BmrFI CCNGG 1 cut(s) 354
BmsI GCATC 2 cut(s) 347, 378
BpiI GAAGAC 1 cut(s) 528
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 227
BsaJI CCNNGG 3 cut(s) 116, 352, 606
BsaWI WCCGGW 1 cut(s) 343
Bsc4I CCNNNNNNNGG 1 cut(s) 301
Bse118I RCCGGY 1 cut(s) 343
Bse3DI GCAATG 1 cut(s) 426
BseBI CCWGG 1 cut(s) 354
BseDI CCNNGG 3 cut(s) 116, 352, 606
BseGI GGATG 1 cut(s) 362
BseLI CCNNNNNNNGG 1 cut(s) 301
BseMI GCAATG 1 cut(s) 426
BseMII CTCAG 1 cut(s) 492
BseXI GCAGC 2 cut(s) 94, 593
BsgI GTGCAG 1 cut(s) 93
Bsh1236I CGCG 1 cut(s) 289
Bsh1285I CGRYCG 1 cut(s) 238
BshFI GGCC 1 cut(s) 371
BshNI GGYRCC 1 cut(s) 4
BshTI ACCGGT 1 cut(s) 343
BsiEI CGRYCG 1 cut(s) 238
BsiHKCI CYCGRG 1 cut(s) 128
BsiSI CCGG 2 cut(s) 344, 572
BslI CCNNNNNNNGG 1 cut(s) 301
BsmAI GTCTC 1 cut(s) 227
BsmI GAATGC 1 cut(s) 601
BsnI GGCC 1 cut(s) 371
Bso31I GGTCTC 1 cut(s) 227
BsoBI CYCGRG 1 cut(s) 128
Bsp143I GATC 8 cut(s) 98, 124, 348, 400, 423, 445, 504, 610
Bsp19I CCATGG 2 cut(s) 116, 606
BspACI CCGC 3 cut(s) 287, 485, 508
BspANI GGCC 1 cut(s) 371
BspCNI CTCAG 1 cut(s) 493
BspFNI CGCG 1 cut(s) 289
BspLI GGNNCC 2 cut(s) 6, 342
BspMAI CTGCAG 1 cut(s) 583
BspPI GGATC 4 cut(s) 343, 440, 499, 618
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 1 cut(s) 227
BsrDI GCAATG 1 cut(s) 426
BsrFI RCCGGY 1 cut(s) 343
BssAI RCCGGY 1 cut(s) 343
BssECI CCNNGG 3 cut(s) 116, 352, 606
BssMI GATC 8 cut(s) 98, 124, 348, 400, 423, 445, 504, 610
BssNAI GTATAC 1 cut(s) 262
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 2 cut(s) 116, 606
Bst1107I GTATAC 1 cut(s) 262
Bst2UI CCWGG 1 cut(s) 354
Bst4CI ACNGT 2 cut(s) 239, 308
BstACI GRCGYC 1 cut(s) 5
BstDEI CTNAG 2 cut(s) 501, 641
BstDSI CCRYGG 2 cut(s) 116, 606
BstENI CCTNNNNNAGG 1 cut(s) 299
BstF5I GGATG 1 cut(s) 362
BstFNI CGCG 1 cut(s) 289
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 440
BstKTI GATC 8 cut(s) 101, 127, 351, 403, 426, 448, 507, 613
BstMAI GTCTC 1 cut(s) 227
BstMBI GATC 8 cut(s) 98, 124, 348, 400, 423, 445, 504, 610
BstMCI CGRYCG 1 cut(s) 238
BstMWI GCNNNNNNNGC 3 cut(s) 437, 581, 590
BstNI CCWGG 1 cut(s) 354
BstNSI RCATGY 1 cut(s) 50
BstSCI CCNGG 1 cut(s) 352
BstSFI CTRYAG 1 cut(s) 579
BstUI CGCG 1 cut(s) 289
BstV1I GCAGC 2 cut(s) 94, 593
BstV2I GAAGAC 1 cut(s) 528
BstX2I RGATCY 2 cut(s) 504, 610
BstYI RGATCY 2 cut(s) 504, 610
BstZ17I GTATAC 1 cut(s) 262
BsuI GTATCC 1 cut(s) 69
BsuRI GGCC 1 cut(s) 371
BtgI CCRYGG 2 cut(s) 116, 606
BtsCI GGATG 1 cut(s) 362
BtsIMutI CAGTG 2 cut(s) 313, 481
CaiI CAGNNNCTG 1 cut(s) 107
CfoI GCGC 2 cut(s) 7, 440
Cfr10I RCCGGY 1 cut(s) 343
Cfr13I GGNCC 2 cut(s) 137, 369
CseI GACGC 1 cut(s) 599
Csp6I GTAC 1 cut(s) 291
CspAI ACCGGT 1 cut(s) 343
CviAII CATG 5 cut(s) 47, 88, 117, 122, 607
CviJI RGCY 9 cut(s) 33, 107, 146, 371, 377, 481, 500, 554, 584
CviKI_1 RGCY 9 cut(s) 33, 107, 146, 371, 377, 481, 500, 554, 584
CviQI GTAC 1 cut(s) 291
DdeI CTNAG 2 cut(s) 501, 641
DinI GGCGCC 1 cut(s) 6
DpnI GATC 8 cut(s) 100, 126, 350, 402, 425, 447, 506, 612
DpnII GATC 8 cut(s) 98, 124, 348, 400, 423, 445, 504, 610
EciI GGCGGA 2 cut(s) 474, 497
Eco130I CCWWGG 2 cut(s) 116, 606
Eco31I GGTCTC 1 cut(s) 227
Eco47I GGWCC 1 cut(s) 137
Eco57I CTGAAG 1 cut(s) 162
Eco88I CYCGRG 1 cut(s) 128
EcoNI CCTNNNNNAGG 1 cut(s) 299
EcoO109I RGGNCCY 1 cut(s) 137
EcoRII CCWGG 1 cut(s) 352
EcoT14I CCWWGG 2 cut(s) 116, 606
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 116, 606
FaeI CATG 5 cut(s) 50, 91, 120, 125, 610
FatI CATG 5 cut(s) 46, 87, 116, 121, 606
FbaI TGATCA 1 cut(s) 400
FblI GTMKAC 2 cut(s) 234, 261
Fnu4HI GCNGC 2 cut(s) 108, 582
FokI GGATG 1 cut(s) 369
Fsp4HI GCNGC 2 cut(s) 108, 582
GlaI GCGC 2 cut(s) 6, 439
GluI GCNGC 2 cut(s) 108, 582
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 371
HapII CCGG 2 cut(s) 344, 572
HgaI GACGC 1 cut(s) 599
HhaI GCGC 2 cut(s) 7, 440
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 5 cut(s) 50, 91, 120, 125, 610
Hin6I GCGC 2 cut(s) 5, 438
HinP1I GCGC 2 cut(s) 5, 438
HincII GTYRAC 2 cut(s) 235, 559
HindII GTYRAC 2 cut(s) 235, 559
HinfI GANTC 1 cut(s) 91
HpaI GTTAAC 1 cut(s) 559
HpaII CCGG 2 cut(s) 344, 572
HphI GGTGA 3 cut(s) 205, 358, 419
Hpy166II GTNNAC 3 cut(s) 235, 262, 559
Hpy188I TCNGA 1 cut(s) 387
Hpy188III TCNNGA 3 cut(s) 128, 130, 449
Hpy8I GTNNAC 3 cut(s) 235, 262, 559
Hpy99I CGWCG 2 cut(s) 593, 632
HpyAV CCTTC 1 cut(s) 578
HpyCH4III ACNGT 2 cut(s) 239, 308
HpyCH4IV ACGT 3 cut(s) 36, 627, 646
HpyCH4V TGCA 4 cut(s) 110, 337, 581, 620
HpyF10VI GCNNNNNNNGC 3 cut(s) 437, 581, 590
HpyF3I CTNAG 2 cut(s) 501, 641
HpySE526I ACGT 3 cut(s) 36, 627, 646
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 5 cut(s) 50, 91, 120, 125, 610
HspAI GCGC 2 cut(s) 5, 438
KasI GGCGCC 1 cut(s) 4
Ksp22I TGATCA 1 cut(s) 400
KspAI GTTAAC 1 cut(s) 559
Kzo9I GATC 8 cut(s) 98, 124, 348, 400, 423, 445, 504, 610
LmnI GCTCC 1 cut(s) 186
LpnPI CCDG 8 cut(s) 153, 323, 339, 357, 366, 389, 462, 585
Lsp1109I GCAGC 2 cut(s) 94, 593
LweI GCATC 2 cut(s) 347, 378
MaeII ACGT 3 cut(s) 36, 627, 646
MaeIII GTNAC 2 cut(s) 92, 308
MalI GATC 8 cut(s) 100, 126, 350, 402, 425, 447, 506, 612
MboI GATC 8 cut(s) 98, 124, 348, 400, 423, 445, 504, 610
MboII GAAGA 3 cut(s) 148, 356, 533
MfeI CAATTG 1 cut(s) 160
MflI RGATCY 2 cut(s) 504, 610
MluCI AATT 2 cut(s) 150, 160
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 100
MnlI CCTC 4 cut(s) 10, 66, 295, 498
MseI TTAA 3 cut(s) 66, 228, 558
MslI CAYNNNNRTG 2 cut(s) 115, 273
MspI CCGG 2 cut(s) 344, 572
MspR9I CCNGG 1 cut(s) 354
MunI CAATTG 1 cut(s) 160
Mva1269I GAATGC 1 cut(s) 601
MvaI CCWGG 1 cut(s) 354
MvnI CGCG 1 cut(s) 289
MwoI GCNNNNNNNGC 3 cut(s) 437, 581, 590
NarI GGCGCC 1 cut(s) 5
NcoI CCATGG 2 cut(s) 116, 606
NdeII GATC 8 cut(s) 98, 124, 348, 400, 423, 445, 504, 610
NlaIII CATG 5 cut(s) 50, 91, 120, 125, 610
NlaIV GGNNCC 2 cut(s) 6, 342
NmuCI GTSAC 2 cut(s) 92, 308
NspI RCATGY 1 cut(s) 50
PaeR7I CTCGAG 1 cut(s) 128
PciI ACATGT 1 cut(s) 46
PctI GAATGC 1 cut(s) 601
PflFI GACNNNGTC 1 cut(s) 626
PinAI ACCGGT 1 cut(s) 343
PkrI GCNGC 2 cut(s) 109, 583
PleI GAGTC 1 cut(s) 99
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 99
PpuMI RGGWCCY 1 cut(s) 137
PscI ACATGT 1 cut(s) 46
Psp5II RGGWCCY 1 cut(s) 137
Psp6I CCWGG 1 cut(s) 352
PspGI CCWGG 1 cut(s) 352
PspN4I GGNNCC 2 cut(s) 6, 342
PspPI GGNCC 2 cut(s) 137, 369
PspPPI RGGWCCY 1 cut(s) 137
PstI CTGCAG 1 cut(s) 583
PstNI CAGNNNCTG 1 cut(s) 107
PsuI RGATCY 2 cut(s) 504, 610
PsyI GACNNNGTC 1 cut(s) 626
RsaI GTAC 1 cut(s) 292
RsaNI GTAC 1 cut(s) 291
RseI CAYNNNNRTG 2 cut(s) 115, 273
SalI GTCGAC 1 cut(s) 233
SaqAI TTAA 3 cut(s) 66, 228, 558
SatI GCNGC 2 cut(s) 108, 582
Sau3AI GATC 8 cut(s) 98, 124, 348, 400, 423, 445, 504, 610
Sau96I GGNCC 2 cut(s) 137, 369
SchI GAGTC 1 cut(s) 100
ScrFI CCNGG 1 cut(s) 354
SfaNI GCATC 2 cut(s) 347, 378
SfcI CTRYAG 1 cut(s) 579
SfoI GGCGCC 1 cut(s) 6
Sfr274I CTCGAG 1 cut(s) 128
SinI GGWCC 1 cut(s) 137
SlaI CTCGAG 1 cut(s) 128
SmiMI CAYNNNNRTG 2 cut(s) 115, 273
SmlI CTYRAG 1 cut(s) 128
SmoI CTYRAG 1 cut(s) 128
Sse9I AATT 2 cut(s) 150, 160
SsiI CCGC 3 cut(s) 287, 485, 508
SspDI GGCGCC 1 cut(s) 4
StyD4I CCNGG 1 cut(s) 352
StyI CCWWGG 2 cut(s) 116, 606
TaaI ACNGT 2 cut(s) 239, 308
TaiI ACGT 3 cut(s) 39, 630, 649
TaqI TCGA 3 cut(s) 129, 207, 234
TasI AATT 2 cut(s) 150, 160
Tru1I TTAA 3 cut(s) 66, 228, 558
Tru9I TTAA 3 cut(s) 66, 228, 558
TscAI CASTG 2 cut(s) 313, 481
TseFI GTSAC 2 cut(s) 92, 308
TseI GCWGC 2 cut(s) 107, 581
Tsp45I GTSAC 2 cut(s) 92, 308
TspDTI ATGAA 2 cut(s) 216, 271
TspRI CASTG 2 cut(s) 313, 481
Tth111I GACNNNGTC 1 cut(s) 626
VpaK11BI GGWCC 1 cut(s) 137
XagI CCTNNNNNAGG 1 cut(s) 299
XceI RCATGY 1 cut(s) 50
XhoI CTCGAG 1 cut(s) 128
XmiI GTMKAC 2 cut(s) 234, 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.