MD04G1120600.v1.1

Soluble inorganic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0007013
Physical Location & Seq
Forward (+)
20720288 .. 20724240
3953 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1120600.v1.1.491

Sequence Viewer

Length: 960 bp
ATGAGCACCAAAACTTGGGAATTTTTCACTTTAATTTTTCGATTTTTTTGCCCTTTAAATGGGAAGGCACAAAGAGAGCGGGAACACGTAGATATACAGCTGCTTATTCCTTTCATCCTCTACTTGAGCGCCCCATTCACTCGTCCTCCTTTCGCTCTTTCAATTTTTGAGATCTGCGCTCTCTTCCTCTTGAAGCTTCCGCTTCAATCCACCGACCGATTCGCAGCTCCGATCCTCCGCCGGTACGTTATCGCCTCGCTTTCCCCACTCCATATGGCTCCACCGATTGAGACTGTTCAAAAAGTTCAGCCTCCACAAAATGCTATTGAACCCCATGTCACCTATCATCATGATCATTCCTCACATCCACCTCTAAATGAGAGGATACTTTCATCCATGACCAGGAGGTCTATCGCTGCCCACCCGTGGCATGACCTTGAAATCGGACCTGGAGCTCCTAAGATTTTCAACTGTGTTATCGAAATTCCAAAAGGAAGCAAAGTGAAATATGAGCTTGACAAAAAAACTGGACTCATCAAGGTTGACCGCATACTGTACTCATCTGTTGTCTACCCCCATAACTATGGTTTCATCCCCCGTACTCTTTGTGAGGATAATGACCCCTTGGATGTCTTGATTATTATGCAGGAGCCAGTTGTTCCTGGATGCTTTCTTCGTGCCAAAGCTATTGGCCTGATGCCTATGATTGACCAGGGTGAGAAAGATGACAAGATCATTGCTGTTTGTGCTGATGATCCTGAGTACCGACACTACAATGACATCAAGGAGCTCCCACCTCACCGTTTGGCTGAGATCCGGCGCTTCTTTGAGGACTACAAGAAAAATGAGAACAAAGAAGTTGCAGTTGATGACTTTCTCCCTGCATCTACTGCCTTTGATGCAATCCAGCATTCCATGAACCTCTATGCGGACTACATTGTGGAGAGCCTTAGGCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0008150 GO:0010035 GO:0010038 GO:0042221 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Pyrophosphatase

Protein Analysis

320

Amino Acids

36.73

Weight (kDa)

6.5

Isoelectric Point (pI)

49.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyrophosphatase PF00719 158 - 309 7.7e-60 Inorganic pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 406
AccB7I CCANNNNNTGG 1 cut(s) 15
AccBSI CCGCTC 1 cut(s) 79
AccI GTMKAC 1 cut(s) 570
AciI CCGC 6 cut(s) 79, 200, 238, 547, 929, 955
AclWI GGATC 3 cut(s) 226, 749, 808
AcsI RAATTY 2 cut(s) 20, 483
AfaI GTAC 4 cut(s) 245, 557, 601, 764
AfiI CCNNNNNNNGG 5 cut(s) 15, 59, 402, 426, 928
AflIII ACRYGT 1 cut(s) 85
AgsI TTSAA 7 cut(s) 162, 193, 206, 299, 329, 440, 469
AjnI CCWGG 4 cut(s) 401, 448, 661, 711
AluBI AGCT 7 cut(s) 100, 196, 227, 455, 514, 686, 790
AluI AGCT 7 cut(s) 100, 196, 227, 455, 514, 686, 790
Alw21I GWGCWC 3 cut(s) 8, 457, 792
Alw26I GTCTC 1 cut(s) 284
AlwI GGATC 3 cut(s) 226, 749, 808
AoxI GGCC 1 cut(s) 691
ApeKI GCWGC 3 cut(s) 100, 224, 416
ApoI RAATTY 2 cut(s) 20, 483
AspLEI GCGC 3 cut(s) 131, 179, 822
AspS9I GGNCC 1 cut(s) 446
AsuHPI GGTGA 3 cut(s) 331, 728, 791
AvaII GGWCC 1 cut(s) 446
AxyI CCTNAGG 1 cut(s) 950
BanII GRGCYC 2 cut(s) 457, 792
Bbv12I GWGCWC 3 cut(s) 8, 457, 792
BbvI GCAGC 3 cut(s) 87, 236, 403
BcgI CGANNNNNNTGC 2 cut(s) 30, 64
BciT130I CCWGG 4 cut(s) 403, 450, 663, 713
BciVI GTATCC 1 cut(s) 378
BclI TGATCA 1 cut(s) 352
BcoDI GTCTC 1 cut(s) 284
BfoI RGCGCY 2 cut(s) 132, 823
BfuI GTATCC 1 cut(s) 378
BglII AGATCT 1 cut(s) 171
BisI GCNGC 3 cut(s) 101, 225, 417
BlsI GCNGC 3 cut(s) 102, 226, 418
Bme1390I CCNGG 4 cut(s) 403, 450, 663, 713
Bme18I GGWCC 1 cut(s) 446
BmgT120I GGNCC 1 cut(s) 446
BmiI GGNNCC 2 cut(s) 279, 651
BmrFI CCNGG 4 cut(s) 403, 450, 663, 713
BmsI GCATC 4 cut(s) 656, 687, 889, 893
BpmI CTGGAG 1 cut(s) 471
BpuEI CTTGAG 1 cut(s) 145
BsaAI YACGTR 1 cut(s) 88
BsaJI CCNNGG 3 cut(s) 425, 624, 712
BsaXI ACNNNNNCTCC 2 cut(s) 130, 160
Bsc4I CCNNNNNNNGG 5 cut(s) 15, 59, 402, 426, 928
Bse118I RCCGGY 1 cut(s) 240
Bse1I ACTGG 2 cut(s) 532, 653
Bse21I CCTNAGG 1 cut(s) 950
Bse3DI GCAATG 1 cut(s) 735
BseBI CCWGG 4 cut(s) 403, 450, 663, 713
BseDI CCNNGG 3 cut(s) 425, 624, 712
BseGI GGATG 6 cut(s) 114, 364, 392, 591, 634, 671
BseLI CCNNNNNNNGG 5 cut(s) 15, 59, 402, 426, 928
BseMI GCAATG 1 cut(s) 735
BseMII CTCAG 2 cut(s) 750, 801
BseNI ACTGG 2 cut(s) 532, 653
BseXI GCAGC 3 cut(s) 87, 236, 403
Bsh1285I CGRYCG 1 cut(s) 217
BshFI GGCC 1 cut(s) 693
BsiEI CGRYCG 1 cut(s) 217
BsiHKAI GWGCWC 3 cut(s) 8, 457, 792
BsiSI CCGG 2 cut(s) 241, 817
BslI CCNNNNNNNGG 5 cut(s) 15, 59, 402, 426, 928
BsmAI GTCTC 1 cut(s) 284
BsmI GAATGC 1 cut(s) 910
BsnI GGCC 1 cut(s) 693
Bsp1286I GDGCHC 3 cut(s) 8, 457, 792
Bsp143I GATC 6 cut(s) 171, 231, 352, 732, 754, 813
BspACI CCGC 6 cut(s) 79, 200, 238, 547, 929, 955
BspANI GGCC 1 cut(s) 693
BspCNI CTCAG 2 cut(s) 751, 802
BspHI TCATGA 1 cut(s) 349
BspLI GGNNCC 2 cut(s) 279, 651
BspPI GGATC 3 cut(s) 226, 749, 808
BsrBI CCGCTC 1 cut(s) 79
BsrDI GCAATG 1 cut(s) 735
BsrFI RCCGGY 1 cut(s) 240
BsrI ACTGG 2 cut(s) 532, 653
BssAI RCCGGY 1 cut(s) 240
BssECI CCNNGG 3 cut(s) 425, 624, 712
BssMI GATC 6 cut(s) 171, 231, 352, 732, 754, 813
BssT1I CCWWGG 1 cut(s) 624
Bst2UI CCWGG 4 cut(s) 403, 450, 663, 713
Bst4CI ACNGT 4 cut(s) 295, 473, 555, 803
Bst6I CTCTTC 1 cut(s) 188
BstAPI GCANNNNNTGC 1 cut(s) 890
BstBAI YACGTR 1 cut(s) 88
BstDEI CTNAG 4 cut(s) 459, 759, 810, 950
BstDSI CCRYGG 1 cut(s) 425
BstF5I GGATG 6 cut(s) 114, 364, 392, 591, 634, 671
BstH2I RGCGCY 2 cut(s) 132, 823
BstHHI GCGC 3 cut(s) 131, 179, 822
BstKTI GATC 6 cut(s) 174, 234, 355, 735, 757, 816
BstMAI GTCTC 1 cut(s) 284
BstMBI GATC 6 cut(s) 171, 231, 352, 732, 754, 813
BstMCI CGRYCG 1 cut(s) 217
BstMWI GCNNNNNNNGC 3 cut(s) 746, 890, 899
BstNI CCWGG 4 cut(s) 403, 450, 663, 713
BstSCI CCNGG 4 cut(s) 401, 448, 661, 711
BstV1I GCAGC 3 cut(s) 87, 236, 403
BstX2I RGATCY 2 cut(s) 171, 813
BstXI CCANNNNNNTGG 1 cut(s) 584
BstYI RGATCY 2 cut(s) 171, 813
Bsu36I CCTNAGG 1 cut(s) 950
BsuI GTATCC 1 cut(s) 378
BsuRI GGCC 1 cut(s) 693
BtgI CCRYGG 1 cut(s) 425
BtsCI GGATG 6 cut(s) 114, 364, 392, 591, 634, 671
CciI TCATGA 1 cut(s) 349
CfoI GCGC 3 cut(s) 131, 179, 822
Cfr10I RCCGGY 1 cut(s) 240
Cfr13I GGNCC 1 cut(s) 446
Csp6I GTAC 4 cut(s) 244, 556, 600, 763
CviAII CATG 5 cut(s) 335, 350, 397, 431, 916
CviQI GTAC 4 cut(s) 244, 556, 600, 763
DdeI CTNAG 4 cut(s) 459, 759, 810, 950
DpnI GATC 6 cut(s) 173, 233, 354, 734, 756, 815
DpnII GATC 6 cut(s) 171, 231, 352, 732, 754, 813
DraI TTTAAA 1 cut(s) 57
DrdI GACNNNNNNGTC 1 cut(s) 406
DseDI GACNNNNNNGTC 1 cut(s) 406
Eam1104I CTCTTC 1 cut(s) 188
EarI CTCTTC 1 cut(s) 188
EciI GGCGGA 1 cut(s) 227
Ecl136II GAGCTC 2 cut(s) 455, 790
Eco130I CCWWGG 1 cut(s) 624
Eco24I GRGCYC 2 cut(s) 457, 792
Eco47I GGWCC 1 cut(s) 446
Eco53kI GAGCTC 2 cut(s) 455, 790
Eco81I CCTNAGG 1 cut(s) 950
EcoICRI GAGCTC 2 cut(s) 455, 790
EcoRII CCWGG 4 cut(s) 401, 448, 661, 711
EcoT14I CCWWGG 1 cut(s) 624
EcoT38I GRGCYC 2 cut(s) 457, 792
ErhI CCWWGG 1 cut(s) 624
FaeI CATG 5 cut(s) 338, 353, 400, 434, 919
FatI CATG 5 cut(s) 334, 349, 396, 430, 915
FauI CCCGC 1 cut(s) 72
FauNDI CATATG 1 cut(s) 273
FbaI TGATCA 1 cut(s) 352
FblI GTMKAC 1 cut(s) 570
Fnu4HI GCNGC 3 cut(s) 101, 225, 417
FokI GGATG 6 cut(s) 101, 351, 379, 578, 641, 678
FriOI GRGCYC 2 cut(s) 457, 792
Fsp4HI GCNGC 3 cut(s) 101, 225, 417
GlaI GCGC 3 cut(s) 130, 178, 821
GluI GCNGC 3 cut(s) 101, 225, 417
GsuI CTGGAG 1 cut(s) 471
HaeII RGCGCY 2 cut(s) 132, 823
HaeIII GGCC 1 cut(s) 693
HapII CCGG 2 cut(s) 241, 817
HhaI GCGC 3 cut(s) 131, 179, 822
Hin1II CATG 5 cut(s) 338, 353, 400, 434, 919
Hin6I GCGC 3 cut(s) 129, 177, 820
HinP1I GCGC 3 cut(s) 129, 177, 820
HincII GTYRAC 1 cut(s) 544
HindII GTYRAC 1 cut(s) 544
HindIII AAGCTT 1 cut(s) 194
HinfI GANTC 2 cut(s) 219, 531
HpaII CCGG 2 cut(s) 241, 817
HphI GGTGA 3 cut(s) 331, 728, 791
Hpy166II GTNNAC 2 cut(s) 544, 571
Hpy188I TCNGA 2 cut(s) 231, 446
Hpy188III TCNNGA 4 cut(s) 190, 350, 634, 758
Hpy8I GTNNAC 2 cut(s) 544, 571
HpyAV CCTTC 1 cut(s) 58
HpyCH4III ACNGT 4 cut(s) 295, 473, 555, 803
HpyCH4IV ACGT 2 cut(s) 87, 246
HpyCH4V TGCA 4 cut(s) 646, 863, 884, 902
HpyF10VI GCNNNNNNNGC 3 cut(s) 746, 890, 899
HpyF3I CTNAG 4 cut(s) 459, 759, 810, 950
HpySE526I ACGT 2 cut(s) 87, 246
Hsp92II CATG 5 cut(s) 338, 353, 400, 434, 919
HspAI GCGC 3 cut(s) 129, 177, 820
Ksp22I TGATCA 1 cut(s) 352
Kzo9I GATC 6 cut(s) 171, 231, 352, 732, 754, 813
LmnI GCTCC 7 cut(s) 232, 283, 452, 460, 649, 787, 795
Lsp1109I GCAGC 3 cut(s) 87, 236, 403
LweI GCATC 4 cut(s) 656, 687, 889, 893
MaeII ACGT 2 cut(s) 87, 246
MaeIII GTNAC 1 cut(s) 337
MalI GATC 6 cut(s) 173, 233, 354, 734, 756, 815
MbiI CCGCTC 1 cut(s) 79
MboI GATC 6 cut(s) 171, 231, 352, 732, 754, 813
MboII GAAGA 2 cut(s) 175, 665
MflI RGATCY 2 cut(s) 171, 813
MhlI GDGCHC 3 cut(s) 8, 457, 792
MluCI AATT 4 cut(s) 20, 33, 162, 483
MlyI GAGTC 1 cut(s) 525
MseI TTAA 2 cut(s) 32, 56
MslI CAYNNNNRTG 2 cut(s) 582, 774
MspA1I CMGCKG 1 cut(s) 100
MspI CCGG 2 cut(s) 241, 817
MspR9I CCNGG 4 cut(s) 403, 450, 663, 713
Mva1269I GAATGC 1 cut(s) 910
MvaI CCWGG 4 cut(s) 403, 450, 663, 713
MwoI GCNNNNNNNGC 3 cut(s) 746, 890, 899
NdeI CATATG 1 cut(s) 273
NdeII GATC 6 cut(s) 171, 231, 352, 732, 754, 813
NlaIII CATG 5 cut(s) 338, 353, 400, 434, 919
NlaIV GGNNCC 2 cut(s) 279, 651
NmuCI GTSAC 1 cut(s) 337
PagI TCATGA 1 cut(s) 349
PctI GAATGC 1 cut(s) 910
PfeI GAWTC 1 cut(s) 219
PflMI CCANNNNNTGG 1 cut(s) 15
PfoI TCCNGGA 1 cut(s) 661
PkrI GCNGC 3 cut(s) 102, 226, 418
PleI GAGTC 1 cut(s) 525
PpsI GAGTC 1 cut(s) 525
Ppu21I YACGTR 1 cut(s) 88
Psp124BI GAGCTC 2 cut(s) 457, 792
Psp6I CCWGG 4 cut(s) 401, 448, 661, 711
PspGI CCWGG 4 cut(s) 401, 448, 661, 711
PspN4I GGNNCC 2 cut(s) 279, 651
PspPI GGNCC 1 cut(s) 446
PsuI RGATCY 2 cut(s) 171, 813
PvuII CAGCTG 1 cut(s) 100
RsaI GTAC 4 cut(s) 245, 557, 601, 764
RsaNI GTAC 4 cut(s) 244, 556, 600, 763
RseI CAYNNNNRTG 2 cut(s) 582, 774
SacI GAGCTC 2 cut(s) 457, 792
SaqAI TTAA 2 cut(s) 32, 56
SatI GCNGC 3 cut(s) 101, 225, 417
Sau3AI GATC 6 cut(s) 171, 231, 352, 732, 754, 813
Sau96I GGNCC 1 cut(s) 446
SchI GAGTC 1 cut(s) 525
ScrFI CCNGG 4 cut(s) 403, 450, 663, 713
SduI GDGCHC 3 cut(s) 8, 457, 792
SfaNI GCATC 4 cut(s) 656, 687, 889, 893
SinI GGWCC 1 cut(s) 446
SmiMI CAYNNNNRTG 2 cut(s) 582, 774
SmlI CTYRAG 1 cut(s) 124
SmoI CTYRAG 1 cut(s) 124
Sse9I AATT 4 cut(s) 20, 33, 162, 483
SsiI CCGC 6 cut(s) 79, 200, 238, 547, 929, 955
SstI GAGCTC 2 cut(s) 457, 792
StyD4I CCNGG 4 cut(s) 401, 448, 661, 711
StyI CCWWGG 1 cut(s) 624
TaaI ACNGT 4 cut(s) 295, 473, 555, 803
TaiI ACGT 2 cut(s) 90, 249
TaqI TCGA 2 cut(s) 40, 480
TaqII GACCGA 1 cut(s) 231
TasI AATT 4 cut(s) 20, 33, 162, 483
TatI WGTACW 1 cut(s) 555
TfiI GAWTC 1 cut(s) 219
Tru1I TTAA 2 cut(s) 32, 56
Tru9I TTAA 2 cut(s) 32, 56
TseFI GTSAC 1 cut(s) 337
TseI GCWGC 3 cut(s) 100, 224, 416
Tsp45I GTSAC 1 cut(s) 337
TspDTI ATGAA 4 cut(s) 103, 381, 580, 932
Van91I CCANNNNNTGG 1 cut(s) 15
VpaK11BI GGWCC 1 cut(s) 446
XapI RAATTY 2 cut(s) 20, 483
XmiI GTMKAC 1 cut(s) 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.