MD12G1137900.v1.1

Soluble inorganic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0007013
Physical Location & Seq
Forward (+)
21556041 .. 21559790
3750 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1137900.v1.1.491

Sequence Viewer

Length: 942 bp
ATGAGCACCAAAACTTGGGAAATTTTCACTTTAACTTTTCATTTATTTTATTTTATTTATTTATTTTCCTTTAAATGGGAAAGCACAAAGAGAGCGGGAACACGTACAGCTGCTTCTACCTTTCATATTCTGCTTGAGCGCCCCATTCACCCCCACTTCCTTCCTCTCTTTCAATTTTTTAGATCTGCGCTCTCTTCCTCTCGAAGCTTTCTCCTCGCTCCGCCGACGGATTTTCAGCTCCGTTCCTCCGCCGATATGGCTCCACCAATTGAGACAGTTCAGAAAGTTGAGCCTCCTCAAAAAGTTGTGGAATCCCATGTCACCTATCATCATGATCACTCCTCGCATCCACCTCTTAATGAGAGGATACTTTCATCCATGACCAGGAGGTCTATCGCTGCTCACCCATGGCATGACCTTGAAATCGGACCTGGAGCTCCTAAGATTTTCAACTGTGTTATCGAAATCCCAAAAGGAAGCAAGGTGAAATATGAGCTTGACAAGAAAACTGGACTCATCAAGGTTGACCGCATATTGTACTCATCTGTTGTCTACCCCCACAACTATGGCTTCATCCCCCGTACCCTTTGTGAGGACAATGATCCCTTGGATGTCTTGATTATTATGCAGGAGCCAGTTGTTCCTGGATGCTTTCTTCGTGCCAAAGCTATTGGCCTGATGCCTATGATTGATCAGGGTGAGAAAGATGACAAGATCATTGCTGTTTGTGCTGATGATCCTGAGTACCGACACTACAATGACATCAAGGAGCTCCCACCTCACCGTTTGGCTGAGATCCGGCGCTTCTTTGAGGACTACAAGAAAAATGAGAACAAAGAAGTTGCAGTTGACGACTTTCTCCCTGCAGCTACTGCCTTTGACGCAATCCAGCATTCCATGAACCTCTATGCGGACTACATTGTGGAGAGCCTCAGGCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0008150 GO:0010035 GO:0010038 GO:0042221 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Pyrophosphatase

Protein Analysis

314

Amino Acids

36.13

Weight (kDa)

6.57

Isoelectric Point (pI)

51.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyrophosphatase PF00719 152 - 303 6.1e-60 Inorganic pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 388
AccB7I CCANNNNNTGG 1 cut(s) 15
AccBSI CCGCTC 1 cut(s) 95
AccI GTMKAC 1 cut(s) 552
AciI CCGC 6 cut(s) 95, 221, 249, 529, 911, 937
AclWI GGATC 3 cut(s) 596, 731, 790
AcsI RAATTY 1 cut(s) 21
AfaI GTAC 4 cut(s) 106, 539, 583, 746
AfiI CCNNNNNNNGG 5 cut(s) 15, 75, 384, 592, 910
AflIII ACRYGT 1 cut(s) 101
AgsI TTSAA 3 cut(s) 173, 422, 451
AjnI CCWGG 3 cut(s) 383, 430, 643
AluBI AGCT 8 cut(s) 110, 207, 238, 437, 496, 668, 772, 869
AluI AGCT 8 cut(s) 110, 207, 238, 437, 496, 668, 772, 869
Alw21I GWGCWC 3 cut(s) 8, 439, 774
Alw26I GTCTC 1 cut(s) 266
AlwI GGATC 3 cut(s) 596, 731, 790
AlwNI CAGNNNCTG 1 cut(s) 872
AoxI GGCC 1 cut(s) 673
ApeKI GCWGC 3 cut(s) 110, 398, 866
ApoI RAATTY 1 cut(s) 21
AspLEI GCGC 3 cut(s) 141, 190, 804
AspS9I GGNCC 1 cut(s) 428
AsuHPI GGTGA 6 cut(s) 140, 313, 395, 496, 710, 773
AvaII GGWCC 1 cut(s) 428
AxyI CCTNAGG 1 cut(s) 932
BanII GRGCYC 2 cut(s) 439, 774
BarI GAAGNNNNNNTAC 2 cut(s) 97, 129
Bbv12I GWGCWC 3 cut(s) 8, 439, 774
BbvI GCAGC 3 cut(s) 97, 385, 878
BciT130I CCWGG 3 cut(s) 385, 432, 645
BciVI GTATCC 1 cut(s) 360
BclI TGATCA 2 cut(s) 334, 691
BcoDI GTCTC 1 cut(s) 266
BfmI CTRYAG 1 cut(s) 864
BfoI RGCGCY 2 cut(s) 142, 805
BfuI GTATCC 1 cut(s) 360
BglI GCCNNNNNGGC 1 cut(s) 257
BglII AGATCT 1 cut(s) 182
BisI GCNGC 3 cut(s) 111, 399, 867
BlsI GCNGC 3 cut(s) 112, 400, 868
Bme1390I CCNGG 3 cut(s) 385, 432, 645
Bme18I GGWCC 1 cut(s) 428
BmgT120I GGNCC 1 cut(s) 428
BmiI GGNNCC 2 cut(s) 261, 633
BmrFI CCNGG 3 cut(s) 385, 432, 645
BmsI GCATC 3 cut(s) 355, 638, 669
BpmI CTGGAG 1 cut(s) 453
BpuEI CTTGAG 1 cut(s) 155
BsaAI YACGTR 1 cut(s) 104
BsaJI CCNNGG 2 cut(s) 407, 606
Bsc4I CCNNNNNNNGG 5 cut(s) 15, 75, 384, 592, 910
Bse1I ACTGG 2 cut(s) 514, 635
Bse21I CCTNAGG 1 cut(s) 932
Bse3DI GCAATG 1 cut(s) 717
BseBI CCWGG 3 cut(s) 385, 432, 645
BseDI CCNNGG 2 cut(s) 407, 606
BseGI GGATG 5 cut(s) 346, 374, 573, 616, 653
BseLI CCNNNNNNNGG 5 cut(s) 15, 75, 384, 592, 910
BseMI GCAATG 1 cut(s) 717
BseMII CTCAG 2 cut(s) 732, 783
BseNI ACTGG 2 cut(s) 514, 635
BseRI GAGGAG 3 cut(s) 203, 285, 331
BseXI GCAGC 3 cut(s) 97, 385, 878
BshFI GGCC 1 cut(s) 675
BsiHKAI GWGCWC 3 cut(s) 8, 439, 774
BsiSI CCGG 1 cut(s) 799
BslI CCNNNNNNNGG 5 cut(s) 15, 75, 384, 592, 910
BsmAI GTCTC 1 cut(s) 266
BsmI GAATGC 1 cut(s) 892
BsnI GGCC 1 cut(s) 675
Bsp1286I GDGCHC 3 cut(s) 8, 439, 774
Bsp143I GATC 7 cut(s) 182, 334, 601, 691, 714, 736, 795
Bsp19I CCATGG 1 cut(s) 407
BspACI CCGC 6 cut(s) 95, 221, 249, 529, 911, 937
BspANI GGCC 1 cut(s) 675
BspCNI CTCAG 2 cut(s) 733, 784
BspHI TCATGA 1 cut(s) 331
BspLI GGNNCC 2 cut(s) 261, 633
BspMAI CTGCAG 1 cut(s) 868
BspPI GGATC 3 cut(s) 596, 731, 790
BsrBI CCGCTC 1 cut(s) 95
BsrDI GCAATG 1 cut(s) 717
BsrI ACTGG 2 cut(s) 514, 635
BssECI CCNNGG 2 cut(s) 407, 606
BssMI GATC 7 cut(s) 182, 334, 601, 691, 714, 736, 795
BssT1I CCWWGG 2 cut(s) 407, 606
Bst2UI CCWGG 3 cut(s) 385, 432, 645
Bst4CI ACNGT 3 cut(s) 277, 455, 785
Bst6I CTCTTC 1 cut(s) 199
BstAPI GCANNNNNTGC 1 cut(s) 872
BstBAI YACGTR 1 cut(s) 104
BstDEI CTNAG 4 cut(s) 441, 741, 792, 932
BstDSI CCRYGG 1 cut(s) 407
BstENI CCTNNNNNAGG 1 cut(s) 590
BstF5I GGATG 5 cut(s) 346, 374, 573, 616, 653
BstH2I RGCGCY 2 cut(s) 142, 805
BstHHI GCGC 3 cut(s) 141, 190, 804
BstKTI GATC 7 cut(s) 185, 337, 604, 694, 717, 739, 798
BstMAI GTCTC 1 cut(s) 266
BstMBI GATC 7 cut(s) 182, 334, 601, 691, 714, 736, 795
BstMWI GCNNNNNNNGC 4 cut(s) 257, 728, 872, 881
BstNI CCWGG 3 cut(s) 385, 432, 645
BstSCI CCNGG 3 cut(s) 383, 430, 643
BstSFI CTRYAG 1 cut(s) 864
BstV1I GCAGC 3 cut(s) 97, 385, 878
BstX2I RGATCY 2 cut(s) 182, 795
BstXI CCANNNNNNTGG 1 cut(s) 566
BstYI RGATCY 2 cut(s) 182, 795
Bsu36I CCTNAGG 1 cut(s) 932
BsuI GTATCC 1 cut(s) 360
BsuRI GGCC 1 cut(s) 675
BtgI CCRYGG 1 cut(s) 407
BtsCI GGATG 5 cut(s) 346, 374, 573, 616, 653
CaiI CAGNNNCTG 1 cut(s) 872
CciI TCATGA 1 cut(s) 331
CfoI GCGC 3 cut(s) 141, 190, 804
Cfr13I GGNCC 1 cut(s) 428
CseI GACGC 1 cut(s) 890
Csp6I GTAC 4 cut(s) 105, 538, 582, 745
CviAII CATG 6 cut(s) 317, 332, 379, 408, 413, 898
CviQI GTAC 4 cut(s) 105, 538, 582, 745
DdeI CTNAG 4 cut(s) 441, 741, 792, 932
DpnI GATC 7 cut(s) 184, 336, 603, 693, 716, 738, 797
DpnII GATC 7 cut(s) 182, 334, 601, 691, 714, 736, 795
DraI TTTAAA 1 cut(s) 73
DrdI GACNNNNNNGTC 1 cut(s) 388
DseDI GACNNNNNNGTC 1 cut(s) 388
Eam1104I CTCTTC 1 cut(s) 199
EarI CTCTTC 1 cut(s) 199
EciI GGCGGA 2 cut(s) 210, 238
Ecl136II GAGCTC 2 cut(s) 437, 772
Eco130I CCWWGG 2 cut(s) 407, 606
Eco24I GRGCYC 2 cut(s) 439, 774
Eco47I GGWCC 1 cut(s) 428
Eco53kI GAGCTC 2 cut(s) 437, 772
Eco81I CCTNAGG 1 cut(s) 932
EcoICRI GAGCTC 2 cut(s) 437, 772
EcoNI CCTNNNNNAGG 1 cut(s) 590
EcoRII CCWGG 3 cut(s) 383, 430, 643
EcoT14I CCWWGG 2 cut(s) 407, 606
EcoT38I GRGCYC 2 cut(s) 439, 774
ErhI CCWWGG 2 cut(s) 407, 606
FaeI CATG 6 cut(s) 320, 335, 382, 411, 416, 901
FatI CATG 6 cut(s) 316, 331, 378, 407, 412, 897
FauI CCCGC 1 cut(s) 88
FbaI TGATCA 2 cut(s) 334, 691
FblI GTMKAC 1 cut(s) 552
Fnu4HI GCNGC 3 cut(s) 111, 399, 867
FokI GGATG 5 cut(s) 333, 361, 560, 623, 660
FriOI GRGCYC 2 cut(s) 439, 774
Fsp4HI GCNGC 3 cut(s) 111, 399, 867
GlaI GCGC 3 cut(s) 140, 189, 803
GluI GCNGC 3 cut(s) 111, 399, 867
GsuI CTGGAG 1 cut(s) 453
HaeII RGCGCY 2 cut(s) 142, 805
HaeIII GGCC 1 cut(s) 675
HapII CCGG 1 cut(s) 799
HgaI GACGC 1 cut(s) 890
HhaI GCGC 3 cut(s) 141, 190, 804
Hin1II CATG 6 cut(s) 320, 335, 382, 411, 416, 901
Hin6I GCGC 3 cut(s) 139, 188, 802
HinP1I GCGC 3 cut(s) 139, 188, 802
HincII GTYRAC 2 cut(s) 526, 850
HindII GTYRAC 2 cut(s) 526, 850
HindIII AAGCTT 1 cut(s) 205
HinfI GANTC 2 cut(s) 311, 513
HpaII CCGG 1 cut(s) 799
HphI GGTGA 6 cut(s) 140, 313, 395, 496, 710, 773
Hpy166II GTNNAC 3 cut(s) 526, 553, 850
Hpy188I TCNGA 2 cut(s) 282, 428
Hpy188III TCNNGA 4 cut(s) 201, 332, 616, 740
Hpy8I GTNNAC 3 cut(s) 526, 553, 850
Hpy99I CGWCG 1 cut(s) 229
HpyAV CCTTC 1 cut(s) 170
HpyCH4III ACNGT 3 cut(s) 277, 455, 785
HpyCH4IV ACGT 1 cut(s) 103
HpyCH4V TGCA 3 cut(s) 628, 845, 866
HpyF10VI GCNNNNNNNGC 4 cut(s) 257, 728, 872, 881
HpyF3I CTNAG 4 cut(s) 441, 741, 792, 932
HpySE526I ACGT 1 cut(s) 103
Hsp92II CATG 6 cut(s) 320, 335, 382, 411, 416, 901
HspAI GCGC 3 cut(s) 139, 188, 802
Ksp22I TGATCA 2 cut(s) 334, 691
Kzo9I GATC 7 cut(s) 182, 334, 601, 691, 714, 736, 795
LmnI GCTCC 8 cut(s) 223, 243, 265, 434, 442, 631, 769, 777
Lsp1109I GCAGC 3 cut(s) 97, 385, 878
LweI GCATC 3 cut(s) 355, 638, 669
MaeII ACGT 1 cut(s) 103
MaeIII GTNAC 1 cut(s) 319
MalI GATC 7 cut(s) 184, 336, 603, 693, 716, 738, 797
MbiI CCGCTC 1 cut(s) 95
MboI GATC 7 cut(s) 182, 334, 601, 691, 714, 736, 795
MboII GAAGA 2 cut(s) 186, 647
MfeI CAATTG 1 cut(s) 267
MflI RGATCY 2 cut(s) 182, 795
MhlI GDGCHC 3 cut(s) 8, 439, 774
MluCI AATT 3 cut(s) 21, 173, 267
MlyI GAGTC 1 cut(s) 507
MseI TTAA 3 cut(s) 32, 72, 357
MslI CAYNNNNRTG 2 cut(s) 564, 756
MspA1I CMGCKG 1 cut(s) 110
MspI CCGG 1 cut(s) 799
MspR9I CCNGG 3 cut(s) 385, 432, 645
MunI CAATTG 1 cut(s) 267
Mva1269I GAATGC 1 cut(s) 892
MvaI CCWGG 3 cut(s) 385, 432, 645
MwoI GCNNNNNNNGC 4 cut(s) 257, 728, 872, 881
NcoI CCATGG 1 cut(s) 407
NdeII GATC 7 cut(s) 182, 334, 601, 691, 714, 736, 795
NlaIII CATG 6 cut(s) 320, 335, 382, 411, 416, 901
NlaIV GGNNCC 2 cut(s) 261, 633
NmuCI GTSAC 1 cut(s) 319
PagI TCATGA 1 cut(s) 331
PctI GAATGC 1 cut(s) 892
PfeI GAWTC 1 cut(s) 311
PflMI CCANNNNNTGG 1 cut(s) 15
PfoI TCCNGGA 1 cut(s) 643
PkrI GCNGC 3 cut(s) 112, 400, 868
PleI GAGTC 1 cut(s) 507
PpsI GAGTC 1 cut(s) 507
Ppu21I YACGTR 1 cut(s) 104
Psp124BI GAGCTC 2 cut(s) 439, 774
Psp6I CCWGG 3 cut(s) 383, 430, 643
PspGI CCWGG 3 cut(s) 383, 430, 643
PspN4I GGNNCC 2 cut(s) 261, 633
PspPI GGNCC 1 cut(s) 428
PstI CTGCAG 1 cut(s) 868
PstNI CAGNNNCTG 1 cut(s) 872
PsuI RGATCY 2 cut(s) 182, 795
PvuII CAGCTG 1 cut(s) 110
RsaI GTAC 4 cut(s) 106, 539, 583, 746
RsaNI GTAC 4 cut(s) 105, 538, 582, 745
RseI CAYNNNNRTG 2 cut(s) 564, 756
SacI GAGCTC 2 cut(s) 439, 774
SaqAI TTAA 3 cut(s) 32, 72, 357
SatI GCNGC 3 cut(s) 111, 399, 867
Sau3AI GATC 7 cut(s) 182, 334, 601, 691, 714, 736, 795
Sau96I GGNCC 1 cut(s) 428
SchI GAGTC 1 cut(s) 507
ScrFI CCNGG 3 cut(s) 385, 432, 645
SduI GDGCHC 3 cut(s) 8, 439, 774
SfaNI GCATC 3 cut(s) 355, 638, 669
SfcI CTRYAG 1 cut(s) 864
SinI GGWCC 1 cut(s) 428
SmiMI CAYNNNNRTG 2 cut(s) 564, 756
SmlI CTYRAG 1 cut(s) 134
SmoI CTYRAG 1 cut(s) 134
Sse9I AATT 3 cut(s) 21, 173, 267
SsiI CCGC 6 cut(s) 95, 221, 249, 529, 911, 937
SstI GAGCTC 2 cut(s) 439, 774
StyD4I CCNGG 3 cut(s) 383, 430, 643
StyI CCWWGG 2 cut(s) 407, 606
TaaI ACNGT 3 cut(s) 277, 455, 785
TaiI ACGT 1 cut(s) 106
TaqI TCGA 2 cut(s) 202, 462
TasI AATT 3 cut(s) 21, 173, 267
TatI WGTACW 1 cut(s) 537
TfiI GAWTC 1 cut(s) 311
Tru1I TTAA 3 cut(s) 32, 72, 357
Tru9I TTAA 3 cut(s) 32, 72, 357
TseFI GTSAC 1 cut(s) 319
TseI GCWGC 3 cut(s) 110, 398, 866
Tsp45I GTSAC 1 cut(s) 319
TspDTI ATGAA 5 cut(s) 29, 113, 363, 562, 914
TspGWI ACGGA 2 cut(s) 230, 242
Van91I CCANNNNNTGG 1 cut(s) 15
VpaK11BI GGWCC 1 cut(s) 428
XagI CCTNNNNNAGG 1 cut(s) 590
XapI RAATTY 1 cut(s) 21
XmiI GTMKAC 1 cut(s) 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.