FvH4_6g14350

Soluble inorganic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0007013
Physical Location & Seq
Reverse (-)
8781007 .. 8785142
4136 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g14350.t3

Sequence Viewer

Length: 741 bp
ATGGCTCCACCCATTGAGGCTACTCAGACAGTTACTGAGATTCCCCAAAAAGTTACTGAAATTCCTCAAAAGGTTGTTGAGGCTTCTAAGAAAGTTACCGAGACTCCCAACAAGGGTGCCAACCATTATTCCTCACATCCACCTCTTAATGAGAGGATCCTTTCTTCCATGACCAGGAGGTCTATTGCTGCACACCCTTGGCATGATCTTGAAATAGGCCCCGGAGCTCCAAAGATCTTCAACTGTGTTGTCGAAATCGGAAAAGGAAGCAAGGTGAAATATGAACTTGACAAAAAAACTGGACTTATCAAGGTTGACCGTATTCTGTACTCATCTGTTGTCTACCCCCACAACTATGGCTTCATCCCCCGTACACTTTGTGAGGACAATGATCCCTTGGATTGCTTGATTATTATGCAGGAGCCGGTTCTTCCTGGATGCTTTCTTCGGGCTAAAGCTATTGGCCTGATGCCCATGATTGATCAGATGACAAGATCATTGCTGTCTGTGCTGATGATCCTGAATACCGTCACTACAGTGACATCAAGGATCTGCCACCACATCGTTTGGCTGAGATCCGTCGCTTCTTTGAGGACTACAAGAAAAATGAGAACAAGGAGGTTGCAGTCGACGACTTTCTCCCTGCATCTGCTGCCTTTGAAAGTATCCAGCAGTCAATGGATCTTTATGCAGACTACATTGTGGAGAGCCTCAGGCGGTAGTTGTTCGTGCGAGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0008150 GO:0010035 GO:0010038 GO:0042221 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Pyrophosphatase

Protein Analysis

247

Amino Acids

27.64

Weight (kDa)

9.49

Isoelectric Point (pI)

55.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyrophosphatase PF00719 82 - 233 3e-58 Inorganic pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 178
AccB1I GGYRCC 1 cut(s) 116
AccI GTMKAC 2 cut(s) 342, 629
AciI CCGC 1 cut(s) 717
AclWI GGATC 7 cut(s) 151, 164, 386, 511, 557, 570, 689
AcsI RAATTY 1 cut(s) 60
AdeI CACNNNGTG 1 cut(s) 380
AfaI GTAC 2 cut(s) 329, 373
AfiI CCNNNNNNNGG 2 cut(s) 113, 174
AgsI TTSAA 3 cut(s) 212, 241, 661
AjnI CCWGG 2 cut(s) 173, 433
AleI CACNNNNGTG 1 cut(s) 536
AluBI AGCT 2 cut(s) 227, 458
AluI AGCT 2 cut(s) 227, 458
Alw21I GWGCWC 1 cut(s) 229
Alw26I GTCTC 1 cut(s) 95
AlwI GGATC 7 cut(s) 151, 164, 386, 511, 557, 570, 689
AlwNI CAGNNNCTG 1 cut(s) 35
AoxI GGCC 2 cut(s) 217, 463
ApeKI GCWGC 2 cut(s) 188, 652
ApoI RAATTY 1 cut(s) 60
AspS9I GGNCC 1 cut(s) 218
AsuC2I CCSGG 1 cut(s) 222
AsuHPI GGTGA 1 cut(s) 286
AxyI CCTNAGG 1 cut(s) 712
BamHI GGATCC 1 cut(s) 156
BanI GGYRCC 1 cut(s) 116
BanII GRGCYC 1 cut(s) 229
Bbv12I GWGCWC 1 cut(s) 229
BbvI GCAGC 2 cut(s) 175, 639
BciT130I CCWGG 2 cut(s) 175, 435
BciVI GTATCC 1 cut(s) 676
BclI TGATCA 1 cut(s) 481
BcnI CCSGG 1 cut(s) 222
BcoDI GTCTC 1 cut(s) 95
BfmI CTRYAG 1 cut(s) 534
BfuI GTATCC 1 cut(s) 676
BglII AGATCT 1 cut(s) 234
BisI GCNGC 2 cut(s) 189, 653
BlsI GCNGC 2 cut(s) 190, 654
Bme1390I CCNGG 3 cut(s) 175, 222, 435
BmgT120I GGNCC 1 cut(s) 218
BmiI GGNNCC 5 cut(s) 6, 118, 158, 220, 423
BmrFI CCNGG 3 cut(s) 175, 222, 435
BmsI GCATC 3 cut(s) 428, 459, 655
BpuMI CCSGG 1 cut(s) 222
BsaJI CCNNGG 3 cut(s) 197, 220, 396
BsaXI ACNNNNNCTCC 2 cut(s) 88, 118
Bsc4I CCNNNNNNNGG 2 cut(s) 113, 174
Bse118I RCCGGY 1 cut(s) 424
Bse1I ACTGG 1 cut(s) 304
Bse21I CCTNAGG 1 cut(s) 712
Bse3DI GCAATG 1 cut(s) 497
BseBI CCWGG 2 cut(s) 175, 435
BseDI CCNNGG 3 cut(s) 197, 220, 396
BseGI GGATG 3 cut(s) 136, 363, 443
BseLI CCNNNNNNNGG 2 cut(s) 113, 174
BseMI GCAATG 1 cut(s) 497
BseMII CTCAG 4 cut(s) 27, 38, 563, 726
BseNI ACTGG 1 cut(s) 304
BseXI GCAGC 2 cut(s) 175, 639
BsgI GTGCAG 1 cut(s) 174
BshFI GGCC 2 cut(s) 219, 465
BshNI GGYRCC 1 cut(s) 116
BsiHKAI GWGCWC 1 cut(s) 229
BsiSI CCGG 2 cut(s) 222, 425
BslI CCNNNNNNNGG 2 cut(s) 113, 174
BsmAI GTCTC 1 cut(s) 95
BsnI GGCC 2 cut(s) 219, 465
Bsp1286I GDGCHC 1 cut(s) 229
BspACI CCGC 1 cut(s) 717
BspANI GGCC 2 cut(s) 219, 465
BspCNI CTCAG 4 cut(s) 28, 37, 564, 725
BspLI GGNNCC 5 cut(s) 6, 118, 158, 220, 423
BspPI GGATC 7 cut(s) 151, 164, 386, 511, 557, 570, 689
BspT107I GGYRCC 1 cut(s) 116
BsrDI GCAATG 1 cut(s) 497
BsrFI RCCGGY 1 cut(s) 424
BsrI ACTGG 1 cut(s) 304
BssAI RCCGGY 1 cut(s) 424
BssECI CCNNGG 3 cut(s) 197, 220, 396
BssT1I CCWWGG 2 cut(s) 197, 396
Bst2UI CCWGG 2 cut(s) 175, 435
Bst4CI ACNGT 5 cut(s) 31, 245, 320, 529, 538
BstAPI GCANNNNNTGC 1 cut(s) 652
BstC8I GCNNGC 1 cut(s) 734
BstDEI CTNAG 5 cut(s) 24, 36, 87, 572, 712
BstF5I GGATG 3 cut(s) 136, 363, 443
BstMAI GTCTC 1 cut(s) 95
BstMWI GCNNNNNNNGC 2 cut(s) 508, 652
BstNI CCWGG 2 cut(s) 175, 435
BstSCI CCNGG 3 cut(s) 173, 220, 433
BstSFI CTRYAG 1 cut(s) 534
BstV1I GCAGC 2 cut(s) 175, 639
BstX2I RGATCY 5 cut(s) 156, 234, 549, 575, 681
BstXI CCANNNNNNTGG 1 cut(s) 356
BstYI RGATCY 5 cut(s) 156, 234, 549, 575, 681
Bsu36I CCTNAGG 1 cut(s) 712
BsuI GTATCC 1 cut(s) 676
BsuRI GGCC 2 cut(s) 219, 465
BtsCI GGATG 3 cut(s) 136, 363, 443
BtsIMutI CAGTG 1 cut(s) 543
Cac8I GCNNGC 1 cut(s) 734
CaiI CAGNNNCTG 1 cut(s) 35
Cfr10I RCCGGY 1 cut(s) 424
Cfr13I GGNCC 1 cut(s) 218
Csp6I GTAC 2 cut(s) 328, 372
CspCI CAANNNNNGTGG 2 cut(s) 548, 583
CviAII CATG 3 cut(s) 169, 203, 475
CviQI GTAC 2 cut(s) 328, 372
DdeI CTNAG 5 cut(s) 24, 36, 87, 572, 712
DraIII CACNNNGTG 1 cut(s) 380
DrdI GACNNNNNNGTC 1 cut(s) 178
DseDI GACNNNNNNGTC 1 cut(s) 178
Ecl136II GAGCTC 1 cut(s) 227
Eco130I CCWWGG 2 cut(s) 197, 396
Eco24I GRGCYC 1 cut(s) 229
Eco53kI GAGCTC 1 cut(s) 227
Eco81I CCTNAGG 1 cut(s) 712
EcoICRI GAGCTC 1 cut(s) 227
EcoO109I RGGNCCY 1 cut(s) 218
EcoRII CCWGG 2 cut(s) 173, 433
EcoT14I CCWWGG 2 cut(s) 197, 396
EcoT38I GRGCYC 1 cut(s) 229
ErhI CCWWGG 2 cut(s) 197, 396
FaeI CATG 3 cut(s) 172, 206, 478
FaiI YATR 7 cut(s) 170, 204, 282, 357, 416, 476, 689
FatI CATG 3 cut(s) 168, 202, 474
FbaI TGATCA 1 cut(s) 481
FblI GTMKAC 2 cut(s) 342, 629
Fnu4HI GCNGC 2 cut(s) 189, 653
FokI GGATG 3 cut(s) 123, 350, 450
FriOI GRGCYC 1 cut(s) 229
Fsp4HI GCNGC 2 cut(s) 189, 653
GluI GCNGC 2 cut(s) 189, 653
HaeIII GGCC 2 cut(s) 219, 465
HapII CCGG 2 cut(s) 222, 425
Hin1II CATG 3 cut(s) 172, 206, 478
HincII GTYRAC 2 cut(s) 316, 630
HindII GTYRAC 2 cut(s) 316, 630
HinfI GANTC 2 cut(s) 40, 103
HpaII CCGG 2 cut(s) 222, 425
HphI GGTGA 1 cut(s) 286
Hpy166II GTNNAC 4 cut(s) 316, 343, 374, 630
Hpy188I TCNGA 3 cut(s) 27, 260, 486
Hpy188III TCNNGA 2 cut(s) 209, 520
Hpy8I GTNNAC 4 cut(s) 316, 343, 374, 630
Hpy99I CGWCG 2 cut(s) 584, 634
HpyCH4III ACNGT 5 cut(s) 31, 245, 320, 529, 538
HpyCH4V TGCA 5 cut(s) 191, 418, 625, 646, 691
HpyF10VI GCNNNNNNNGC 2 cut(s) 508, 652
HpyF3I CTNAG 5 cut(s) 24, 36, 87, 572, 712
Hsp92II CATG 3 cut(s) 172, 206, 478
Ksp22I TGATCA 1 cut(s) 481
LmnI GCTCC 4 cut(s) 10, 224, 232, 421
Lsp1109I GCAGC 2 cut(s) 175, 639
LweI GCATC 3 cut(s) 428, 459, 655
MaeIII GTNAC 5 cut(s) 31, 52, 94, 529, 538
MboII GAAGA 4 cut(s) 156, 229, 422, 437
MflI RGATCY 5 cut(s) 156, 234, 549, 575, 681
MhlI GDGCHC 1 cut(s) 229
MluCI AATT 1 cut(s) 60
MlyI GAGTC 1 cut(s) 97
MseI TTAA 1 cut(s) 147
MslI CAYNNNNRTG 2 cut(s) 354, 536
MspI CCGG 2 cut(s) 222, 425
MspR9I CCNGG 3 cut(s) 175, 222, 435
MvaI CCWGG 2 cut(s) 175, 435
MwoI GCNNNNNNNGC 2 cut(s) 508, 652
NciI CCSGG 1 cut(s) 222
NlaIII CATG 3 cut(s) 172, 206, 478
NlaIV GGNNCC 5 cut(s) 6, 118, 158, 220, 423
NmuCI GTSAC 2 cut(s) 529, 538
OliI CACNNNNGTG 1 cut(s) 536
PfeI GAWTC 1 cut(s) 40
PfoI TCCNGGA 1 cut(s) 433
PkrI GCNGC 2 cut(s) 190, 654
PleI GAGTC 1 cut(s) 97
PpsI GAGTC 1 cut(s) 97
Psp124BI GAGCTC 1 cut(s) 229
Psp6I CCWGG 2 cut(s) 173, 433
PspGI CCWGG 2 cut(s) 173, 433
PspN4I GGNNCC 5 cut(s) 6, 118, 158, 220, 423
PspPI GGNCC 1 cut(s) 218
PstNI CAGNNNCTG 1 cut(s) 35
PsuI RGATCY 5 cut(s) 156, 234, 549, 575, 681
RsaI GTAC 2 cut(s) 329, 373
RsaNI GTAC 2 cut(s) 328, 372
RseI CAYNNNNRTG 2 cut(s) 354, 536
SacI GAGCTC 1 cut(s) 229
SalI GTCGAC 1 cut(s) 628
SaqAI TTAA 1 cut(s) 147
SatI GCNGC 2 cut(s) 189, 653
Sau96I GGNCC 1 cut(s) 218
SchI GAGTC 1 cut(s) 97
ScrFI CCNGG 3 cut(s) 175, 222, 435
SduI GDGCHC 1 cut(s) 229
SetI ASST 8 cut(s) 75, 145, 182, 229, 276, 315, 460, 623
SfaNI GCATC 3 cut(s) 428, 459, 655
SfcI CTRYAG 1 cut(s) 534
SmiMI CAYNNNNRTG 2 cut(s) 354, 536
Sse9I AATT 1 cut(s) 60
SsiI CCGC 1 cut(s) 717
SstI GAGCTC 1 cut(s) 229
StyD4I CCNGG 3 cut(s) 173, 220, 433
StyI CCWWGG 2 cut(s) 197, 396
TaaI ACNGT 5 cut(s) 31, 245, 320, 529, 538
TaqI TCGA 2 cut(s) 252, 629
TasI AATT 1 cut(s) 60
TatI WGTACW 1 cut(s) 327
TfiI GAWTC 1 cut(s) 40
Tru1I TTAA 1 cut(s) 147
Tru9I TTAA 1 cut(s) 147
TscAI CASTG 1 cut(s) 543
TseFI GTSAC 2 cut(s) 529, 538
TseI GCWGC 2 cut(s) 188, 652
Tsp45I GTSAC 2 cut(s) 529, 538
TspDTI ATGAA 2 cut(s) 297, 352
TspGWI ACGGA 1 cut(s) 568
TspRI CASTG 1 cut(s) 543
XapI RAATTY 1 cut(s) 60
XmiI GTMKAC 2 cut(s) 342, 629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.