AT3G53630

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0008470
Physical Location & Seq
Forward (+)
19883787 .. 19885938
2152 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G53630.2

Sequence Viewer

Length: 585 bp
ATGAGCTCCTTCAATGGAAACAGCAACATCGACAACCTCCTAATCCAAACCCTACTTGGACGTCTCCAAATTCGTCCCCCTAACTCTCATTTCCTCTCTCAATCCCTAGACGACCTCCTCTTCAAATCCGACGACAGCGACGGCGATGATGACGGAGAAGGTCAGACCAGTCTCGACAGAGAAGAAGCTAGGCTTGAGAAAGAGCTAATCCGAGTCATCGTCTCTGGTCGAAGCGATTCTCTCAAGCCGAATTCAGGTCAAGCCGTGACAGTCAACGAACACCACATCTGCGTTGGGTTTCATGAGGATGAGGAATCGGATTATCGTGTTTGGGAGTGGCATGGTCATATCATGTTGTTTGATGAAGAGAATGGGTACACGCCTGAGTATATCTACGGGAATTACTTCGAGAGGTTGCCTGTGAAGCTTCCTAATAACCGCCGTGTGGAGAAGGAGAAGAAGAAGGAGATGAAAGAAGAGGAGGTGGAGAATTTGGGGCTTAGAGAGTTGATCGATGGTGGTGACGCTGCTCCTGGTAGGATTCTTCATAGGAACAACATCAACATCGGTTCCTCAAGGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

194

Amino Acids

22.21

Weight (kDa)

4.85

Isoelectric Point (pI)

49.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 64
AciI CCGC 1 cut(s) 439
AcsI RAATTY 3 cut(s) 69, 250, 490
AcyI GRCGYC 1 cut(s) 61
AfaI GTAC 1 cut(s) 377
AfiI CCNNNNNNNGG 2 cut(s) 254, 445
AgsI TTSAA 2 cut(s) 13, 124
AjnI CCWGG 1 cut(s) 532
AluBI AGCT 4 cut(s) 6, 188, 205, 427
AluI AGCT 4 cut(s) 6, 188, 205, 427
Alw21I GWGCWC 1 cut(s) 8
Alw26I GTCTC 3 cut(s) 68, 176, 226
ApeKI GCWGC 1 cut(s) 527
ApoI RAATTY 3 cut(s) 69, 250, 490
Asp700I GAANNNNTTC 2 cut(s) 235, 404
AsuHPI GGTGA 1 cut(s) 533
BanII GRGCYC 1 cut(s) 8
Bbv12I GWGCWC 1 cut(s) 8
BbvI GCAGC 1 cut(s) 514
BccI CCATC 1 cut(s) 509
BceAI ACGGC 3 cut(s) 157, 248, 426
BciT130I CCWGG 1 cut(s) 534
BcoDI GTCTC 3 cut(s) 68, 176, 226
BfaI CTAG 2 cut(s) 107, 189
BisI GCNGC 1 cut(s) 528
BlsI GCNGC 1 cut(s) 529
Bme1390I CCNGG 1 cut(s) 534
BmiI GGNNCC 1 cut(s) 571
BmrFI CCNGG 1 cut(s) 534
BpuEI CTTGAG 3 cut(s) 215, 227, 559
Bsa29I ATCGAT 1 cut(s) 513
BsaHI GRCGYC 1 cut(s) 61
Bsc4I CCNNNNNNNGG 2 cut(s) 254, 445
Bse1I ACTGG 1 cut(s) 168
BseBI CCWGG 1 cut(s) 534
BseCI ATCGAT 1 cut(s) 513
BseGI GGATG 1 cut(s) 313
BseLI CCNNNNNNNGG 2 cut(s) 254, 445
BseMII CTCAG 1 cut(s) 375
BseNI ACTGG 1 cut(s) 168
BseRI GAGGAG 2 cut(s) 107, 494
BseXI GCAGC 1 cut(s) 514
BshVI ATCGAT 1 cut(s) 513
BsiHKAI GWGCWC 1 cut(s) 8
BslFI GGGAC 1 cut(s) 60
BslI CCNNNNNNNGG 2 cut(s) 254, 445
BsmAI GTCTC 3 cut(s) 68, 176, 226
BsmBI CGTCTC 2 cut(s) 68, 226
BsmFI GGGAC 1 cut(s) 60
Bsp1286I GDGCHC 1 cut(s) 8
Bsp143I GATC 1 cut(s) 510
BspACI CCGC 1 cut(s) 439
BspCNI CTCAG 1 cut(s) 376
BspDI ATCGAT 1 cut(s) 513
BspHI TCATGA 1 cut(s) 301
BspLI GGNNCC 1 cut(s) 571
BsrI ACTGG 1 cut(s) 168
BssMI GATC 1 cut(s) 510
BssNI GRCGYC 1 cut(s) 61
Bst2UI CCWGG 1 cut(s) 534
Bst4CI ACNGT 1 cut(s) 271
Bst6I CTCTTC 3 cut(s) 125, 360, 471
BstACI GRCGYC 1 cut(s) 61
BstDEI CTNAG 2 cut(s) 384, 500
BstF5I GGATG 1 cut(s) 313
BstKTI GATC 1 cut(s) 513
BstMAI GTCTC 3 cut(s) 68, 176, 226
BstMBI GATC 1 cut(s) 510
BstMWI GCNNNNNNNGC 1 cut(s) 424
BstNI CCWGG 1 cut(s) 534
BstSCI CCNGG 1 cut(s) 532
BstV1I GCAGC 1 cut(s) 514
Bsu15I ATCGAT 1 cut(s) 513
BsuTUI ATCGAT 1 cut(s) 513
BtgZI GCGATG 1 cut(s) 159
BtsCI GGATG 1 cut(s) 313
CciI TCATGA 1 cut(s) 301
ClaI ATCGAT 1 cut(s) 513
CseI GACGC 1 cut(s) 533
Csp6I GTAC 1 cut(s) 376
CviAII CATG 3 cut(s) 302, 341, 352
CviJI RGCY 8 cut(s) 6, 188, 193, 205, 247, 263, 427, 499
CviKI_1 RGCY 8 cut(s) 6, 188, 193, 205, 247, 263, 427, 499
CviQI GTAC 1 cut(s) 376
DdeI CTNAG 2 cut(s) 384, 500
DpnI GATC 1 cut(s) 512
DpnII GATC 1 cut(s) 510
Eam1104I CTCTTC 3 cut(s) 125, 360, 471
EarI CTCTTC 3 cut(s) 125, 360, 471
Ecl136II GAGCTC 1 cut(s) 6
Eco24I GRGCYC 1 cut(s) 8
Eco53kI GAGCTC 1 cut(s) 6
EcoICRI GAGCTC 1 cut(s) 6
EcoRI GAATTC 1 cut(s) 250
EcoRII CCWGG 1 cut(s) 532
EcoT38I GRGCYC 1 cut(s) 8
Esp3I CGTCTC 2 cut(s) 68, 226
FaeI CATG 3 cut(s) 305, 344, 355
FaiI YATR 6 cut(s) 303, 342, 348, 353, 390, 549
FalI AAGNNNNNCTT 2 cut(s) 177, 209
FaqI GGGAC 1 cut(s) 60
FatI CATG 3 cut(s) 301, 340, 351
Fnu4HI GCNGC 1 cut(s) 528
FokI GGATG 1 cut(s) 320
FriOI GRGCYC 1 cut(s) 8
Fsp4HI GCNGC 1 cut(s) 528
FspBI CTAG 2 cut(s) 107, 189
GluI GCNGC 1 cut(s) 528
HgaI GACGC 1 cut(s) 533
Hin1I GRCGYC 1 cut(s) 61
Hin1II CATG 3 cut(s) 305, 344, 355
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HindIII AAGCTT 1 cut(s) 425
HinfI GANTC 4 cut(s) 213, 236, 314, 541
HphI GGTGA 1 cut(s) 533
Hpy166II GTNNAC 2 cut(s) 274, 378
Hpy188I TCNGA 4 cut(s) 130, 165, 212, 319
Hpy188III TCNNGA 3 cut(s) 173, 302, 409
Hpy8I GTNNAC 2 cut(s) 274, 378
Hpy99I CGWCG 2 cut(s) 134, 143
HpyAV CCTTC 4 cut(s) 19, 152, 445, 457
HpyCH4III ACNGT 1 cut(s) 271
HpyCH4IV ACGT 1 cut(s) 61
HpyF10VI GCNNNNNNNGC 1 cut(s) 424
HpyF3I CTNAG 2 cut(s) 384, 500
HpySE526I ACGT 1 cut(s) 61
Hsp92I GRCGYC 1 cut(s) 61
Hsp92II CATG 3 cut(s) 305, 344, 355
Kzo9I GATC 1 cut(s) 510
LmnI GCTCC 2 cut(s) 11, 535
LpnPI CCDG 7 cut(s) 181, 210, 240, 396, 432, 519, 546
Lsp1109I GCAGC 1 cut(s) 514
MaeI CTAG 2 cut(s) 107, 189
MaeII ACGT 1 cut(s) 61
MaeIII GTNAC 2 cut(s) 265, 521
MalI GATC 1 cut(s) 512
MboI GATC 1 cut(s) 510
MboII GAAGA 7 cut(s) 112, 194, 377, 469, 472, 488, 536
MhlI GDGCHC 1 cut(s) 8
MluCI AATT 4 cut(s) 69, 250, 400, 490
MlyI GAGTC 1 cut(s) 222
MmeI TCCRAC 1 cut(s) 153
MroXI GAANNNNTTC 2 cut(s) 235, 404
MslI CAYNNNNRTG 1 cut(s) 306
MspR9I CCNGG 1 cut(s) 534
MvaI CCWGG 1 cut(s) 534
MwoI GCNNNNNNNGC 1 cut(s) 424
NdeII GATC 1 cut(s) 510
NlaIII CATG 3 cut(s) 305, 344, 355
NlaIV GGNNCC 1 cut(s) 571
NmuCI GTSAC 2 cut(s) 265, 521
PagI TCATGA 1 cut(s) 301
PdmI GAANNNNTTC 2 cut(s) 235, 404
PfeI GAWTC 3 cut(s) 236, 314, 541
PkrI GCNGC 1 cut(s) 529
PleI GAGTC 1 cut(s) 221
PpsI GAGTC 1 cut(s) 221
Psp124BI GAGCTC 1 cut(s) 8
Psp6I CCWGG 1 cut(s) 532
PspGI CCWGG 1 cut(s) 532
PspN4I GGNNCC 1 cut(s) 571
RsaI GTAC 1 cut(s) 377
RsaNI GTAC 1 cut(s) 376
RseI CAYNNNNRTG 1 cut(s) 306
SacI GAGCTC 1 cut(s) 8
SatI GCNGC 1 cut(s) 528
Sau3AI GATC 1 cut(s) 510
SchI GAGTC 1 cut(s) 222
ScrFI CCNGG 1 cut(s) 534
SduI GDGCHC 1 cut(s) 8
SmiMI CAYNNNNRTG 1 cut(s) 306
SmlI CTYRAG 3 cut(s) 194, 242, 574
SmoI CTYRAG 3 cut(s) 194, 242, 574
Sse9I AATT 4 cut(s) 69, 250, 400, 490
SsiI CCGC 1 cut(s) 439
SspMI CTAG 2 cut(s) 107, 189
SstI GAGCTC 1 cut(s) 8
StyD4I CCNGG 1 cut(s) 532
TaaI ACNGT 1 cut(s) 271
TaiI ACGT 1 cut(s) 64
TaqI TCGA 5 cut(s) 30, 174, 229, 408, 513
TasI AATT 4 cut(s) 69, 250, 400, 490
TfiI GAWTC 3 cut(s) 236, 314, 541
TseFI GTSAC 2 cut(s) 265, 521
TseI GCWGC 1 cut(s) 527
Tsp45I GTSAC 2 cut(s) 265, 521
TspDTI ATGAA 4 cut(s) 290, 378, 485, 536
TspGWI ACGGA 1 cut(s) 168
XapI RAATTY 3 cut(s) 69, 250, 490
XcmI CCANNNNNNNNNTGG 2 cut(s) 53, 290
XmnI GAANNNNTTC 2 cut(s) 235, 404
XspI CTAG 2 cut(s) 107, 189
ZraI GACGTC 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.