FvH4_6g14340

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0008470
Physical Location & Seq
Reverse (-)
8777058 .. 8779780
2723 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g14340.t1

Sequence Viewer

Length: 633 bp
ATGAGCTCCTTCACCAGCACCACCAACTTCGACAACCTCATGCTCCAGACCCTCATGGGTCGCCTCCAAATCCGACCCCCAACCATTACCACCAACTCCTCCTTCCTCTCCCAAACCCTCGAAGACCTCCTCTACGACGCCGCCAACCTCTCCTCCTCCGACGACGACGAGAACAAAACCCAGCTCGCCAAAGAGGAGTCCAAGCTCGAGCGGGAGATCATCCGGGTCATCCTCACCGGCAACACCGAATCCCTCAAACCCAATTCGGGCCAGGCCGTTACGATCGGCGAGCACCACATTTGCGTCGGGTTTCACGAGGAAACGGGTTCGGATTATCGGGTCTGGGAGTGGCACGGTCATATTATGCTGTTTGATGAAGAGAATGGGTACACGCCGGAGTATATATATGGGAATTACTTTGAGAGGTTGGTTGGGAAGGCGCGTGGCGGAAGCAGCGGCGGTGTTCGTGGCAGTGAGGAGGTGAAAGAGGAAGAGGAGGAGGAGAAAGAGGTGGTTGGGAATATGGGGTTGAGGGAGTTGATTGATGGTGTGGAGGAGGATTCGGGTCAGGCTCGGATTGTTCATCGGAATATCAATGCCAGTGCCGGTTCTACAACTACTACAAGGAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

211

Amino Acids

23.41

Weight (kDa)

4.66

Isoelectric Point (pI)

48.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 211
AccII CGCG 1 cut(s) 442
AciI CCGC 5 cut(s) 141, 211, 447, 456, 459
AcyI GRCGYC 1 cut(s) 138
AfaI GTAC 1 cut(s) 389
AfiI CCNNNNNNNGG 1 cut(s) 266
AjnI CCWGG 1 cut(s) 270
AjuI GAANNNNNNNTTGG 2 cut(s) 86, 118
AluBI AGCT 3 cut(s) 6, 184, 205
AluI AGCT 3 cut(s) 6, 184, 205
Alw21I GWGCWC 2 cut(s) 8, 294
Ama87I CYCGRG 1 cut(s) 206
AoxI GGCC 2 cut(s) 268, 273
ApeKI GCWGC 1 cut(s) 453
AspLEI GCGC 1 cut(s) 442
AspS9I GGNCC 1 cut(s) 268
AsuC2I CCSGG 1 cut(s) 224
AsuHPI GGTGA 3 cut(s) 4, 226, 493
AvaI CYCGRG 1 cut(s) 206
BanII GRGCYC 1 cut(s) 8
BauI CACGAG 1 cut(s) 314
BbsI GAAGAC 1 cut(s) 129
Bbv12I GWGCWC 2 cut(s) 8, 294
BbvI GCAGC 1 cut(s) 465
BccI CCATC 1 cut(s) 539
BceAI ACGGC 1 cut(s) 260
BciT130I CCWGG 1 cut(s) 272
BcnI CCSGG 1 cut(s) 224
BisI GCNGC 3 cut(s) 141, 454, 457
BlsI GCNGC 3 cut(s) 142, 455, 458
Bme1390I CCNGG 2 cut(s) 224, 272
BmeT110I CYCGRG 1 cut(s) 206
BmgT120I GGNCC 1 cut(s) 268
BmrFI CCNGG 2 cut(s) 224, 272
BpiI GAAGAC 1 cut(s) 129
BpmI CTGGAG 1 cut(s) 29
BpuMI CCSGG 1 cut(s) 224
BsaHI GRCGYC 1 cut(s) 138
BsaXI ACNNNNNCTCC 2 cut(s) 137, 167
Bsc4I CCNNNNNNNGG 1 cut(s) 266
Bse118I RCCGGY 2 cut(s) 236, 605
Bse1I ACTGG 1 cut(s) 600
BseBI CCWGG 1 cut(s) 272
BseGI GGATG 2 cut(s) 219, 228
BseLI CCNNNNNNNGG 1 cut(s) 266
BseNI ACTGG 1 cut(s) 600
BseXI GCAGC 1 cut(s) 465
BseYI CCCAGC 1 cut(s) 180
Bsh1236I CGCG 1 cut(s) 442
Bsh1285I CGRYCG 1 cut(s) 285
BshFI GGCC 2 cut(s) 270, 275
BsiEI CGRYCG 1 cut(s) 285
BsiHKAI GWGCWC 2 cut(s) 8, 294
BsiHKCI CYCGRG 1 cut(s) 206
BsiSI CCGG 4 cut(s) 223, 237, 395, 606
BslI CCNNNNNNNGG 1 cut(s) 266
BsnI GGCC 2 cut(s) 270, 275
BsoBI CYCGRG 1 cut(s) 206
Bsp1286I GDGCHC 2 cut(s) 8, 294
Bsp143I GATC 2 cut(s) 216, 282
BspACI CCGC 5 cut(s) 141, 211, 447, 456, 459
BspANI GGCC 2 cut(s) 270, 275
BspFNI CGCG 1 cut(s) 442
BsrBI CCGCTC 1 cut(s) 211
BsrFI RCCGGY 2 cut(s) 236, 605
BsrI ACTGG 1 cut(s) 600
BssAI RCCGGY 2 cut(s) 236, 605
BssMI GATC 2 cut(s) 216, 282
BssNI GRCGYC 1 cut(s) 138
BssSI CACGAG 1 cut(s) 314
Bst2BI CACGAG 1 cut(s) 314
Bst2UI CCWGG 1 cut(s) 272
Bst4CI ACNGT 1 cut(s) 356
Bst6I CTCTTC 2 cut(s) 372, 486
BstACI GRCGYC 1 cut(s) 138
BstC8I GCNNGC 2 cut(s) 186, 290
BstF5I GGATG 2 cut(s) 219, 228
BstFNI CGCG 1 cut(s) 442
BstHHI GCGC 1 cut(s) 442
BstKTI GATC 2 cut(s) 219, 285
BstMBI GATC 2 cut(s) 216, 282
BstMCI CGRYCG 1 cut(s) 285
BstMWI GCNNNNNNNGC 1 cut(s) 453
BstNI CCWGG 1 cut(s) 272
BstSCI CCNGG 2 cut(s) 222, 270
BstUI CGCG 1 cut(s) 442
BstV1I GCAGC 1 cut(s) 465
BstV2I GAAGAC 1 cut(s) 129
BsuRI GGCC 2 cut(s) 270, 275
BtsCI GGATG 2 cut(s) 219, 228
BtsI GCAGTG 1 cut(s) 478
BtsIMutI CAGTG 2 cut(s) 478, 607
Cac8I GCNNGC 2 cut(s) 186, 290
CfoI GCGC 1 cut(s) 442
Cfr10I RCCGGY 2 cut(s) 236, 605
Cfr13I GGNCC 1 cut(s) 268
CseI GACGC 2 cut(s) 146, 292
Csp6I GTAC 1 cut(s) 388
CviAII CATG 2 cut(s) 40, 55
CviJI RGCY 6 cut(s) 6, 184, 205, 270, 275, 572
CviKI_1 RGCY 6 cut(s) 6, 184, 205, 270, 275, 572
CviQI GTAC 1 cut(s) 388
DpnI GATC 2 cut(s) 218, 284
DpnII GATC 2 cut(s) 216, 282
Eam1104I CTCTTC 2 cut(s) 372, 486
EarI CTCTTC 2 cut(s) 372, 486
EciI GGCGGA 1 cut(s) 462
Ecl136II GAGCTC 1 cut(s) 6
Eco24I GRGCYC 1 cut(s) 8
Eco53kI GAGCTC 1 cut(s) 6
Eco88I CYCGRG 1 cut(s) 206
EcoICRI GAGCTC 1 cut(s) 6
EcoRII CCWGG 1 cut(s) 270
EcoT38I GRGCYC 1 cut(s) 8
FaeI CATG 2 cut(s) 43, 58
FaiI YATR 9 cut(s) 41, 56, 360, 365, 402, 404, 406, 408, 524
FatI CATG 2 cut(s) 39, 54
FauI CCCGC 1 cut(s) 204
Fnu4HI GCNGC 3 cut(s) 141, 454, 457
FokI GGATG 2 cut(s) 206, 215
FriOI GRGCYC 1 cut(s) 8
Fsp4HI GCNGC 3 cut(s) 141, 454, 457
GlaI GCGC 1 cut(s) 441
GluI GCNGC 3 cut(s) 141, 454, 457
GsaI CCCAGC 1 cut(s) 184
GsuI CTGGAG 1 cut(s) 29
HaeIII GGCC 2 cut(s) 270, 275
HapII CCGG 4 cut(s) 223, 237, 395, 606
HgaI GACGC 2 cut(s) 146, 292
HhaI GCGC 1 cut(s) 442
Hin1I GRCGYC 1 cut(s) 138
Hin1II CATG 2 cut(s) 43, 58
Hin6I GCGC 1 cut(s) 440
HinP1I GCGC 1 cut(s) 440
HinfI GANTC 3 cut(s) 197, 248, 560
HpaII CCGG 4 cut(s) 223, 237, 395, 606
HphI GGTGA 3 cut(s) 4, 226, 493
Hpy166II GTNNAC 1 cut(s) 390
Hpy188I TCNGA 5 cut(s) 74, 160, 331, 576, 588
Hpy188III TCNNGA 2 cut(s) 46, 314
Hpy8I GTNNAC 1 cut(s) 390
Hpy99I CGWCG 5 cut(s) 140, 164, 167, 170, 308
HpyAV CCTTC 3 cut(s) 19, 112, 430
HpyCH4III ACNGT 1 cut(s) 356
HpyF10VI GCNNNNNNNGC 1 cut(s) 453
Hsp92I GRCGYC 1 cut(s) 138
Hsp92II CATG 2 cut(s) 43, 58
HspAI GCGC 1 cut(s) 440
Kzo9I GATC 2 cut(s) 216, 282
LmnI GCTCC 2 cut(s) 11, 48
Lsp1109I GCAGC 1 cut(s) 465
MaeIII GTNAC 1 cut(s) 277
MalI GATC 2 cut(s) 218, 284
MbiI CCGCTC 1 cut(s) 211
MboI GATC 2 cut(s) 216, 282
MboII GAAGA 3 cut(s) 134, 389, 503
MhlI GDGCHC 2 cut(s) 8, 294
MluCI AATT 2 cut(s) 262, 412
MlyI GAGTC 1 cut(s) 206
MmeI TCCRAC 2 cut(s) 97, 183
MspA1I CMGCKG 1 cut(s) 456
MspI CCGG 4 cut(s) 223, 237, 395, 606
MspR9I CCNGG 2 cut(s) 224, 272
MvaI CCWGG 1 cut(s) 272
MvnI CGCG 1 cut(s) 442
MwoI GCNNNNNNNGC 1 cut(s) 453
NciI CCSGG 1 cut(s) 224
NdeII GATC 2 cut(s) 216, 282
NlaIII CATG 2 cut(s) 43, 58
PaeR7I CTCGAG 1 cut(s) 206
PcsI WCGNNNNNNNCGW 1 cut(s) 312
PfeI GAWTC 2 cut(s) 248, 560
PkrI GCNGC 3 cut(s) 142, 455, 458
Ple19I CGATCG 1 cut(s) 285
PleI GAGTC 1 cut(s) 205
PpsI GAGTC 1 cut(s) 205
Psp124BI GAGCTC 1 cut(s) 8
Psp6I CCWGG 1 cut(s) 270
PspFI CCCAGC 1 cut(s) 180
PspGI CCWGG 1 cut(s) 270
PspPI GGNCC 1 cut(s) 268
PspXI VCTCGAGB 1 cut(s) 206
PvuI CGATCG 1 cut(s) 285
RsaI GTAC 1 cut(s) 389
RsaNI GTAC 1 cut(s) 388
SacI GAGCTC 1 cut(s) 8
SatI GCNGC 3 cut(s) 141, 454, 457
Sau3AI GATC 2 cut(s) 216, 282
Sau96I GGNCC 1 cut(s) 268
SchI GAGTC 1 cut(s) 206
ScrFI CCNGG 2 cut(s) 224, 272
SduI GDGCHC 2 cut(s) 8, 294
SetI ASST 9 cut(s) 8, 39, 129, 150, 186, 207, 428, 483, 513
Sfr274I CTCGAG 1 cut(s) 206
SlaI CTCGAG 1 cut(s) 206
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
Sse9I AATT 2 cut(s) 262, 412
SsiI CCGC 5 cut(s) 141, 211, 447, 456, 459
SstI GAGCTC 1 cut(s) 8
StyD4I CCNGG 2 cut(s) 222, 270
TaaI ACNGT 1 cut(s) 356
TaqI TCGA 3 cut(s) 30, 120, 207
TasI AATT 2 cut(s) 262, 412
TauI GCSGC 2 cut(s) 143, 459
TfiI GAWTC 2 cut(s) 248, 560
TscAI CASTG 2 cut(s) 478, 607
TseI GCWGC 1 cut(s) 453
TspDTI ATGAA 2 cut(s) 390, 572
TspRI CASTG 2 cut(s) 478, 607
XhoI CTCGAG 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.