MD04G1120700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0008470
Physical Location & Seq
Forward (+)
20725698 .. 20728450
2753 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1120700.v1.1.491

Sequence Viewer

Length: 657 bp
ATGAGCTCCTTCACCAACACCACCAATTTCGACAACCTCATGCTCCAAACCCTAATGGGCAGGCTCCAAATCCGACCCCCCACCAACAACTCCTTCCTCTCCCAAACCCTCGAGGATCTTCTCCTCGACGCTGCCAATCTCTCCGCCGACGACGACGATGACGACGAGAACAAGACCCAGCTCGCCAAGGAAGAATCCCGCCTCGAGAAGGATATCATCCGGGTTATTCTCAGCGGCAAGACAGATTCCCTCAAGCCCAATTCGGGTCAGGCCGTCACCATCGGAGAGCACCACATTTGCGTGGGGTTTCATGAGGAATCGGGTTCGGACTATCGGGTTTGGGAGTGGCATGGGCACATCATGCTCTTTGATGAAGAGAACGGGTACACTCCTGAGTATATATATGGGAATTACTTTGAGAGGCTGATTGGTAAGGCTCGTGGAAGTGGTGGCAGCGGAGGTGACAGTGTTCTTGTTGAGGAGGCCAAGGAGGAAGAGGAAGAGGAGGAGGATAAAGAGAAAGTTACTACTTTGGGGCTCAGGGAGTTGATTGATGGCGGGGATTCGGGTCAGGGTCGAATTCTTCATCGGAATATCAATGCGGGTTCTACAAGATTACCTAAAAGAAAGGCTTTTGGTTGCATAAGAAGTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

219

Amino Acids

24.4

Weight (kDa)

4.73

Isoelectric Point (pI)

47.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 110
AciI CCGC 6 cut(s) 144, 199, 234, 456, 558, 602
AclWI GGATC 1 cut(s) 123
AcsI RAATTY 1 cut(s) 579
AfaI GTAC 1 cut(s) 386
AfiI CCNNNNNNNGG 2 cut(s) 208, 263
AjuI GAANNNNNNNTTGG 2 cut(s) 77, 109
AluBI AGCT 2 cut(s) 6, 181
AluI AGCT 2 cut(s) 6, 181
Alw21I GWGCWC 2 cut(s) 8, 291
AlwI GGATC 1 cut(s) 123
Ama87I CYCGRG 2 cut(s) 110, 203
AoxI GGCC 2 cut(s) 270, 483
ApeKI GCWGC 2 cut(s) 131, 453
ApoI RAATTY 1 cut(s) 579
AsuC2I CCSGG 1 cut(s) 221
AsuHPI GGTGA 3 cut(s) 4, 268, 473
AvaI CYCGRG 2 cut(s) 110, 203
BaeGI GKGCMC 1 cut(s) 357
BanII GRGCYC 2 cut(s) 8, 540
BauI CACGAG 1 cut(s) 438
Bbv12I GWGCWC 2 cut(s) 8, 291
BbvI GCAGC 2 cut(s) 118, 465
BccI CCATC 2 cut(s) 287, 548
BceAI ACGGC 1 cut(s) 257
BcnI CCSGG 1 cut(s) 221
BfaI CTAG 1 cut(s) 655
BisI GCNGC 3 cut(s) 132, 235, 454
BlsI GCNGC 3 cut(s) 133, 236, 455
Bme1390I CCNGG 1 cut(s) 221
BmeT110I CYCGRG 2 cut(s) 110, 203
BmiI GGNNCC 1 cut(s) 65
BmrFI CCNGG 1 cut(s) 221
Bpu10I CCTNAGC 1 cut(s) 539
BpuEI CTTGAG 1 cut(s) 236
BpuMI CCSGG 1 cut(s) 221
BsaJI CCNNGG 2 cut(s) 186, 486
Bsc4I CCNNNNNNNGG 2 cut(s) 208, 263
BseDI CCNNGG 2 cut(s) 186, 486
BseGI GGATG 1 cut(s) 216
BseLI CCNNNNNNNGG 2 cut(s) 208, 263
BseMII CTCAG 3 cut(s) 244, 384, 553
BseRI GAGGAG 4 cut(s) 113, 494, 518, 521
BseSI GKGCMC 1 cut(s) 357
BseXI GCAGC 2 cut(s) 118, 465
BseYI CCCAGC 1 cut(s) 177
BshFI GGCC 2 cut(s) 272, 485
BsiHKAI GWGCWC 2 cut(s) 8, 291
BsiHKCI CYCGRG 2 cut(s) 110, 203
BsiSI CCGG 1 cut(s) 220
BslI CCNNNNNNNGG 2 cut(s) 208, 263
BsnI GGCC 2 cut(s) 272, 485
BsoBI CYCGRG 2 cut(s) 110, 203
Bsp1286I GDGCHC 4 cut(s) 8, 291, 357, 540
Bsp143I GATC 1 cut(s) 115
BspACI CCGC 6 cut(s) 144, 199, 234, 456, 558, 602
BspANI GGCC 2 cut(s) 272, 485
BspCNI CTCAG 3 cut(s) 243, 385, 552
BspHI TCATGA 1 cut(s) 310
BspLI GGNNCC 1 cut(s) 65
BspPI GGATC 1 cut(s) 123
BssECI CCNNGG 2 cut(s) 186, 486
BssMI GATC 1 cut(s) 115
BssSI CACGAG 1 cut(s) 438
BssT1I CCWWGG 2 cut(s) 186, 486
Bst2BI CACGAG 1 cut(s) 438
Bst4CI ACNGT 1 cut(s) 467
Bst6I CTCTTC 3 cut(s) 369, 489, 495
BstAPI GCANNNNNTGC 1 cut(s) 361
BstC8I GCNNGC 2 cut(s) 62, 183
BstDEI CTNAG 3 cut(s) 230, 393, 539
BstENI CCTNNNNNAGG 1 cut(s) 206
BstF5I GGATG 1 cut(s) 216
BstKTI GATC 1 cut(s) 118
BstMBI GATC 1 cut(s) 115
BstMWI GCNNNNNNNGC 1 cut(s) 361
BstSCI CCNGG 1 cut(s) 219
BstSLI GKGCMC 1 cut(s) 357
BstV1I GCAGC 2 cut(s) 118, 465
BstX2I RGATCY 1 cut(s) 115
BstYI RGATCY 1 cut(s) 115
BsuRI GGCC 2 cut(s) 272, 485
BtsCI GGATG 1 cut(s) 216
BtsIMutI CAGTG 1 cut(s) 472
Cac8I GCNNGC 2 cut(s) 62, 183
CciI TCATGA 1 cut(s) 310
CseI GACGC 1 cut(s) 137
Csp6I GTAC 1 cut(s) 385
CviAII CATG 4 cut(s) 40, 311, 350, 361
CviQI GTAC 1 cut(s) 385
DdeI CTNAG 3 cut(s) 230, 393, 539
DpnI GATC 1 cut(s) 117
DpnII GATC 1 cut(s) 115
Eam1104I CTCTTC 3 cut(s) 369, 489, 495
EarI CTCTTC 3 cut(s) 369, 489, 495
EciI GGCGGA 1 cut(s) 133
Ecl136II GAGCTC 1 cut(s) 6
Eco130I CCWWGG 2 cut(s) 186, 486
Eco24I GRGCYC 2 cut(s) 8, 540
Eco32I GATATC 1 cut(s) 214
Eco53kI GAGCTC 1 cut(s) 6
Eco88I CYCGRG 2 cut(s) 110, 203
EcoICRI GAGCTC 1 cut(s) 6
EcoNI CCTNNNNNAGG 1 cut(s) 206
EcoRI GAATTC 1 cut(s) 579
EcoRV GATATC 1 cut(s) 214
EcoT14I CCWWGG 2 cut(s) 186, 486
EcoT38I GRGCYC 2 cut(s) 8, 540
ErhI CCWWGG 2 cut(s) 186, 486
FaeI CATG 4 cut(s) 43, 314, 353, 364
FaiI YATR 9 cut(s) 41, 312, 351, 362, 399, 401, 403, 405, 644
FalI AAGNNNNNCTT 2 cut(s) 616, 648
FatI CATG 4 cut(s) 39, 310, 349, 360
FauI CCCGC 3 cut(s) 206, 551, 595
Fnu4HI GCNGC 3 cut(s) 132, 235, 454
FokI GGATG 1 cut(s) 203
FriOI GRGCYC 2 cut(s) 8, 540
Fsp4HI GCNGC 3 cut(s) 132, 235, 454
FspBI CTAG 1 cut(s) 655
GluI GCNGC 3 cut(s) 132, 235, 454
GsaI CCCAGC 1 cut(s) 181
HaeIII GGCC 2 cut(s) 272, 485
HapII CCGG 1 cut(s) 220
HgaI GACGC 1 cut(s) 137
Hin1II CATG 4 cut(s) 43, 314, 353, 364
HinfI GANTC 4 cut(s) 194, 245, 317, 563
HpaII CCGG 1 cut(s) 220
HphI GGTGA 3 cut(s) 4, 268, 473
Hpy166II GTNNAC 1 cut(s) 387
Hpy188I TCNGA 4 cut(s) 74, 284, 328, 591
Hpy188III TCNNGA 3 cut(s) 205, 311, 392
Hpy8I GTNNAC 1 cut(s) 387
Hpy99I CGWCG 5 cut(s) 131, 152, 155, 158, 167
HpyAV CCTTC 3 cut(s) 19, 103, 202
HpyCH4III ACNGT 1 cut(s) 467
HpyCH4V TGCA 1 cut(s) 642
HpyF10VI GCNNNNNNNGC 1 cut(s) 361
HpyF3I CTNAG 3 cut(s) 230, 393, 539
Hsp92II CATG 4 cut(s) 43, 314, 353, 364
Kzo9I GATC 1 cut(s) 115
LmnI GCTCC 3 cut(s) 11, 48, 69
LpnPI CCDG 7 cut(s) 46, 191, 233, 254, 405, 526, 557
Lsp1109I GCAGC 2 cut(s) 118, 465
MaeI CTAG 1 cut(s) 655
MaeIII GTNAC 3 cut(s) 274, 461, 523
MalI GATC 1 cut(s) 117
MboI GATC 1 cut(s) 115
MboII GAAGA 6 cut(s) 110, 203, 386, 506, 512, 575
MflI RGATCY 1 cut(s) 115
MhlI GDGCHC 4 cut(s) 8, 291, 357, 540
MluCI AATT 4 cut(s) 25, 259, 409, 579
MmeI TCCRAC 1 cut(s) 97
MslI CAYNNNNRTG 1 cut(s) 299
MspA1I CMGCKG 2 cut(s) 234, 456
MspI CCGG 1 cut(s) 220
MspR9I CCNGG 1 cut(s) 221
MwoI GCNNNNNNNGC 1 cut(s) 361
NciI CCSGG 1 cut(s) 221
NdeII GATC 1 cut(s) 115
NlaIII CATG 4 cut(s) 43, 314, 353, 364
NlaIV GGNNCC 1 cut(s) 65
NmuCI GTSAC 2 cut(s) 274, 461
PaeR7I CTCGAG 2 cut(s) 110, 203
PagI TCATGA 1 cut(s) 310
PcsI WCGNNNNNNNCGW 2 cut(s) 159, 162
PfeI GAWTC 4 cut(s) 194, 245, 317, 563
PkrI GCNGC 3 cut(s) 133, 236, 455
Psp124BI GAGCTC 1 cut(s) 8
PspFI CCCAGC 1 cut(s) 177
PspN4I GGNNCC 1 cut(s) 65
PspXI VCTCGAGB 1 cut(s) 110
PsuI RGATCY 1 cut(s) 115
RsaI GTAC 1 cut(s) 386
RsaNI GTAC 1 cut(s) 385
RseI CAYNNNNRTG 1 cut(s) 299
SacI GAGCTC 1 cut(s) 8
SatI GCNGC 3 cut(s) 132, 235, 454
Sau3AI GATC 1 cut(s) 115
ScrFI CCNGG 1 cut(s) 221
SduI GDGCHC 4 cut(s) 8, 291, 357, 540
SetI ASST 5 cut(s) 8, 39, 183, 463, 622
Sfr274I CTCGAG 2 cut(s) 110, 203
SlaI CTCGAG 2 cut(s) 110, 203
SmiMI CAYNNNNRTG 1 cut(s) 299
SmlI CTYRAG 3 cut(s) 110, 203, 251
SmoI CTYRAG 3 cut(s) 110, 203, 251
Sse9I AATT 4 cut(s) 25, 259, 409, 579
SsiI CCGC 6 cut(s) 144, 199, 234, 456, 558, 602
SspMI CTAG 1 cut(s) 655
SstI GAGCTC 1 cut(s) 8
StyD4I CCNGG 1 cut(s) 219
StyI CCWWGG 2 cut(s) 186, 486
TaaI ACNGT 1 cut(s) 467
TaqI TCGA 5 cut(s) 30, 111, 126, 204, 577
TasI AATT 4 cut(s) 25, 259, 409, 579
TauI GCSGC 1 cut(s) 237
TfiI GAWTC 4 cut(s) 194, 245, 317, 563
TscAI CASTG 1 cut(s) 472
TseFI GTSAC 2 cut(s) 274, 461
TseI GCWGC 2 cut(s) 131, 453
Tsp45I GTSAC 2 cut(s) 274, 461
TspDTI ATGAA 3 cut(s) 299, 387, 575
TspRI CASTG 1 cut(s) 472
XagI CCTNNNNNAGG 1 cut(s) 206
XapI RAATTY 1 cut(s) 579
XhoI CTCGAG 2 cut(s) 110, 203
XspI CTAG 1 cut(s) 655
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.