Rroxscaffold_6G00413710

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0008470
Physical Location & Seq
Forward (+)
36081504 .. 36082624
1121 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00413710.1

Sequence Viewer

Length: 756 bp
ATGAGCTCCTTCAGCAGCACCACCAACTTTGACAACCTGATGCTCCAAACCCTCATGGGTCGCCTTCAAATCCGACCCCCAACCACCACCGCCAACAACAACTCCTTCCTCTCCCAAACCCTCGAAGACCTCCTCTTCGACGCCGCCAACCTCTCCGCCGACGACGACGACGACGAGAACAAGACCCAGCTCGCCAAGGAGGAGTCCAAGCTCGAGAGGGAGATCATCCGGGTCATCCTCACCGGCAACACCGATTCGCTCAAGCCCAATTCGGGCCAGGCCGTCACGATCGGGGAGCACCACATTTGCGTCGGGTTTCACGAGGAAACGGGTTCGGATTATCGGGTCTGGGAGTGGCACGGCCATATTATGCTGTTTGATGAAGAAAACGGGTACACGCCGGAGTATATATACGGGAATTACTTTGAGAGATTGATTGGGAAGGCGCGTGGTGGAAGTAGCGGCGGCGGCGGTCGTGGTGAAGAGGTGAAAGAGGAGGAGGAGAAAGAGAAGGTGGGGAATTTGGGGCTCAGGGAATTGATTGATGGTGTGGAGGATTCTGGTCTGGCTCGGATTGTTCATCGGAATATCAATGCCAATGCCGGTTCTACAACTAACACAAGTGACTTGTTATACCGTCAAAGTCAGAGGTTCAAGCTTGATGTCACTGATCTAGATCAGGTTGACACCTCCAAAATAGGTCATGATTGGACTAGCAGTTTAATGAAGAGATATGCAAGTGGGATTAGGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

251

Amino Acids

28.05

Weight (kDa)

4.83

Isoelectric Point (pI)

34.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 448
AciI CCGC 7 cut(s) 90, 144, 156, 462, 465, 468, 471
AcoI YGGCCR 1 cut(s) 361
AcsI RAATTY 1 cut(s) 520
AcyI GRCGYC 1 cut(s) 141
AfaI GTAC 1 cut(s) 395
AfiI CCNNNNNNNGG 1 cut(s) 272
AgsI TTSAA 2 cut(s) 68, 655
AjnI CCWGG 1 cut(s) 276
AluBI AGCT 4 cut(s) 6, 190, 211, 658
AluI AGCT 4 cut(s) 6, 190, 211, 658
Alw21I GWGCWC 2 cut(s) 8, 300
Ama87I CYCGRG 1 cut(s) 212
AoxI GGCC 3 cut(s) 274, 279, 361
ApeKI GCWGC 1 cut(s) 15
ApoI RAATTY 1 cut(s) 520
ArsI GACNNNNNNTTYG 2 cut(s) 119, 151
AspLEI GCGC 1 cut(s) 448
AspS9I GGNCC 1 cut(s) 274
AsuC2I CCSGG 1 cut(s) 230
AsuHPI GGTGA 3 cut(s) 232, 491, 499
AvaI CYCGRG 1 cut(s) 212
BanII GRGCYC 2 cut(s) 8, 531
BauI CACGAG 1 cut(s) 320
BbsI GAAGAC 1 cut(s) 132
Bbv12I GWGCWC 2 cut(s) 8, 300
BbvI GCAGC 1 cut(s) 27
BccI CCATC 1 cut(s) 539
BceAI ACGGC 2 cut(s) 266, 376
BciT130I CCWGG 1 cut(s) 278
BcnI CCSGG 1 cut(s) 230
BfaI CTAG 2 cut(s) 674, 714
BisI GCNGC 5 cut(s) 16, 144, 463, 466, 469
BlsI GCNGC 5 cut(s) 17, 145, 464, 467, 470
Bme1390I CCNGG 2 cut(s) 230, 278
BmeT110I CYCGRG 1 cut(s) 212
BmgT120I GGNCC 1 cut(s) 274
BmrFI CCNGG 2 cut(s) 230, 278
BmsI GCATC 1 cut(s) 30
BpiI GAAGAC 1 cut(s) 132
Bpu10I CCTNAGC 1 cut(s) 530
BpuEI CTTGAG 1 cut(s) 245
BpuMI CCSGG 1 cut(s) 230
BsaBI GATNNNNATC 1 cut(s) 675
BsaHI GRCGYC 1 cut(s) 141
BsaJI CCNNGG 1 cut(s) 195
BsaXI ACNNNNNCTCC 2 cut(s) 86, 116
Bsc4I CCNNNNNNNGG 1 cut(s) 272
Bse118I RCCGGY 2 cut(s) 242, 602
Bse8I GATNNNNATC 1 cut(s) 675
BseBI CCWGG 1 cut(s) 278
BseDI CCNNGG 1 cut(s) 195
BseGI GGATG 2 cut(s) 225, 234
BseJI GATNNNNATC 1 cut(s) 675
BseLI CCNNNNNNNGG 1 cut(s) 272
BseMII CTCAG 1 cut(s) 544
BseRI GAGGAG 5 cut(s) 122, 215, 509, 512, 515
BseXI GCAGC 1 cut(s) 27
BseYI CCCAGC 1 cut(s) 186
Bsh1236I CGCG 1 cut(s) 448
Bsh1285I CGRYCG 2 cut(s) 291, 475
BshFI GGCC 3 cut(s) 276, 281, 363
BsiEI CGRYCG 2 cut(s) 291, 475
BsiHKAI GWGCWC 2 cut(s) 8, 300
BsiHKCI CYCGRG 1 cut(s) 212
BsiSI CCGG 4 cut(s) 229, 243, 401, 603
BslI CCNNNNNNNGG 1 cut(s) 272
BsnI GGCC 3 cut(s) 276, 281, 363
BsoBI CYCGRG 1 cut(s) 212
Bsp1286I GDGCHC 3 cut(s) 8, 300, 531
Bsp143I GATC 4 cut(s) 222, 288, 670, 676
BspACI CCGC 7 cut(s) 90, 144, 156, 462, 465, 468, 471
BspANI GGCC 3 cut(s) 276, 281, 363
BspCNI CTCAG 1 cut(s) 543
BspFNI CGCG 1 cut(s) 448
BspHI TCATGA 1 cut(s) 703
BsrFI RCCGGY 2 cut(s) 242, 602
BssAI RCCGGY 2 cut(s) 242, 602
BssECI CCNNGG 1 cut(s) 195
BssMI GATC 4 cut(s) 222, 288, 670, 676
BssNI GRCGYC 1 cut(s) 141
BssSI CACGAG 1 cut(s) 320
BssT1I CCWWGG 1 cut(s) 195
Bst2BI CACGAG 1 cut(s) 320
Bst2UI CCWGG 1 cut(s) 278
Bst4CI ACNGT 1 cut(s) 638
Bst6I CTCTTC 3 cut(s) 140, 477, 722
BstACI GRCGYC 1 cut(s) 141
BstC8I GCNNGC 1 cut(s) 192
BstDEI CTNAG 1 cut(s) 530
BstF5I GGATG 2 cut(s) 225, 234
BstFNI CGCG 1 cut(s) 448
BstHHI GCGC 1 cut(s) 448
BstKTI GATC 4 cut(s) 225, 291, 673, 679
BstMBI GATC 4 cut(s) 222, 288, 670, 676
BstMCI CGRYCG 2 cut(s) 291, 475
BstMWI GCNNNNNNNGC 2 cut(s) 12, 468
BstNI CCWGG 1 cut(s) 278
BstSCI CCNGG 2 cut(s) 228, 276
BstUI CGCG 1 cut(s) 448
BstV1I GCAGC 1 cut(s) 27
BstV2I GAAGAC 1 cut(s) 132
BsuRI GGCC 3 cut(s) 276, 281, 363
BtsCI GGATG 2 cut(s) 225, 234
BtsIMutI CAGTG 1 cut(s) 666
Cac8I GCNNGC 1 cut(s) 192
CciI TCATGA 1 cut(s) 703
CfoI GCGC 1 cut(s) 448
Cfr10I RCCGGY 2 cut(s) 242, 602
Cfr13I GGNCC 1 cut(s) 274
CseI GACGC 2 cut(s) 149, 298
Csp6I GTAC 1 cut(s) 394
CspCI CAANNNNNGTGG 2 cut(s) 10, 45
CviAII CATG 2 cut(s) 55, 704
CviQI GTAC 1 cut(s) 394
DdeI CTNAG 1 cut(s) 530
DpnI GATC 4 cut(s) 224, 290, 672, 678
DpnII GATC 4 cut(s) 222, 288, 670, 676
EaeI YGGCCR 1 cut(s) 361
Eam1104I CTCTTC 3 cut(s) 140, 477, 722
EarI CTCTTC 3 cut(s) 140, 477, 722
EciI GGCGGA 1 cut(s) 145
Ecl136II GAGCTC 1 cut(s) 6
Eco130I CCWWGG 1 cut(s) 195
Eco24I GRGCYC 2 cut(s) 8, 531
Eco53kI GAGCTC 1 cut(s) 6
Eco88I CYCGRG 1 cut(s) 212
EcoICRI GAGCTC 1 cut(s) 6
EcoRII CCWGG 1 cut(s) 276
EcoT14I CCWWGG 1 cut(s) 195
EcoT38I GRGCYC 2 cut(s) 8, 531
ErhI CCWWGG 1 cut(s) 195
FaeI CATG 2 cut(s) 58, 707
FaiI YATR 9 cut(s) 56, 366, 371, 408, 410, 412, 634, 705, 735
FatI CATG 2 cut(s) 54, 703
Fnu4HI GCNGC 5 cut(s) 16, 144, 463, 466, 469
FokI GGATG 2 cut(s) 212, 221
FriOI GRGCYC 2 cut(s) 8, 531
Fsp4HI GCNGC 5 cut(s) 16, 144, 463, 466, 469
FspBI CTAG 2 cut(s) 674, 714
GlaI GCGC 1 cut(s) 447
GluI GCNGC 5 cut(s) 16, 144, 463, 466, 469
GsaI CCCAGC 1 cut(s) 190
HaeIII GGCC 3 cut(s) 276, 281, 363
HapII CCGG 4 cut(s) 229, 243, 401, 603
HgaI GACGC 2 cut(s) 149, 298
HhaI GCGC 1 cut(s) 448
Hin1I GRCGYC 1 cut(s) 141
Hin1II CATG 2 cut(s) 58, 707
Hin6I GCGC 1 cut(s) 446
HinP1I GCGC 1 cut(s) 446
HincII GTYRAC 1 cut(s) 685
HindII GTYRAC 1 cut(s) 685
HindIII AAGCTT 1 cut(s) 656
HinfI GANTC 3 cut(s) 203, 254, 557
HpaII CCGG 4 cut(s) 229, 243, 401, 603
HphI GGTGA 3 cut(s) 232, 491, 499
Hpy166II GTNNAC 2 cut(s) 396, 685
Hpy188I TCNGA 5 cut(s) 74, 337, 573, 585, 648
Hpy188III TCNNGA 5 cut(s) 214, 286, 320, 674, 704
Hpy8I GTNNAC 2 cut(s) 396, 685
Hpy99I CGWCG 7 cut(s) 143, 164, 167, 170, 173, 176, 314
HpyAV CCTTC 5 cut(s) 19, 74, 115, 436, 505
HpyCH4III ACNGT 1 cut(s) 638
HpyCH4V TGCA 1 cut(s) 737
HpyF10VI GCNNNNNNNGC 2 cut(s) 12, 468
HpyF3I CTNAG 1 cut(s) 530
Hsp92I GRCGYC 1 cut(s) 141
Hsp92II CATG 2 cut(s) 58, 707
HspAI GCGC 1 cut(s) 446
Kzo9I GATC 4 cut(s) 222, 288, 670, 676
LmnI GCTCC 3 cut(s) 11, 48, 295
Lsp1109I GCAGC 1 cut(s) 27
LweI GCATC 1 cut(s) 30
MaeI CTAG 2 cut(s) 674, 714
MaeIII GTNAC 3 cut(s) 283, 623, 664
MalI GATC 4 cut(s) 224, 290, 672, 678
MboI GATC 4 cut(s) 222, 288, 670, 676
MboII GAAGA 5 cut(s) 127, 137, 395, 494, 739
MhlI GDGCHC 3 cut(s) 8, 300, 531
MluCI AATT 4 cut(s) 268, 418, 520, 536
MlyI GAGTC 1 cut(s) 212
MmeI TCCRAC 1 cut(s) 97
MseI TTAA 1 cut(s) 722
MspI CCGG 4 cut(s) 229, 243, 401, 603
MspR9I CCNGG 2 cut(s) 230, 278
MvaI CCWGG 1 cut(s) 278
MvnI CGCG 1 cut(s) 448
MwoI GCNNNNNNNGC 2 cut(s) 12, 468
NciI CCSGG 1 cut(s) 230
NdeII GATC 4 cut(s) 222, 288, 670, 676
NlaIII CATG 2 cut(s) 58, 707
NmuCI GTSAC 3 cut(s) 283, 623, 664
PaeR7I CTCGAG 1 cut(s) 212
PagI TCATGA 1 cut(s) 703
PcsI WCGNNNNNNNCGW 3 cut(s) 168, 171, 318
PfeI GAWTC 2 cut(s) 254, 557
PkrI GCNGC 5 cut(s) 17, 145, 464, 467, 470
Ple19I CGATCG 1 cut(s) 291
PleI GAGTC 1 cut(s) 211
PpsI GAGTC 1 cut(s) 211
Psp124BI GAGCTC 1 cut(s) 8
Psp6I CCWGG 1 cut(s) 276
PspFI CCCAGC 1 cut(s) 186
PspGI CCWGG 1 cut(s) 276
PspPI GGNCC 1 cut(s) 274
PvuI CGATCG 1 cut(s) 291
RsaI GTAC 1 cut(s) 395
RsaNI GTAC 1 cut(s) 394
SacI GAGCTC 1 cut(s) 8
SaqAI TTAA 1 cut(s) 722
SatI GCNGC 5 cut(s) 16, 144, 463, 466, 469
Sau3AI GATC 4 cut(s) 222, 288, 670, 676
Sau96I GGNCC 1 cut(s) 274
SchI GAGTC 1 cut(s) 212
ScrFI CCNGG 2 cut(s) 230, 278
SduI GDGCHC 3 cut(s) 8, 300, 531
SfaNI GCATC 1 cut(s) 30
Sfr274I CTCGAG 1 cut(s) 212
SlaI CTCGAG 1 cut(s) 212
SmlI CTYRAG 2 cut(s) 212, 260
SmoI CTYRAG 2 cut(s) 212, 260
Sse9I AATT 4 cut(s) 268, 418, 520, 536
SsiI CCGC 7 cut(s) 90, 144, 156, 462, 465, 468, 471
SspMI CTAG 2 cut(s) 674, 714
SstI GAGCTC 1 cut(s) 8
StyD4I CCNGG 2 cut(s) 228, 276
StyI CCWWGG 1 cut(s) 195
TaaI ACNGT 1 cut(s) 638
TaqI TCGA 3 cut(s) 123, 138, 213
TasI AATT 4 cut(s) 268, 418, 520, 536
TauI GCSGC 4 cut(s) 146, 465, 468, 471
TfiI GAWTC 2 cut(s) 254, 557
Tru1I TTAA 1 cut(s) 722
Tru9I TTAA 1 cut(s) 722
TscAI CASTG 1 cut(s) 673
TseFI GTSAC 3 cut(s) 283, 623, 664
TseI GCWGC 1 cut(s) 15
Tsp45I GTSAC 3 cut(s) 283, 623, 664
TspDTI ATGAA 3 cut(s) 396, 569, 740
TspRI CASTG 1 cut(s) 673
XapI RAATTY 1 cut(s) 520
XbaI TCTAGA 1 cut(s) 673
XhoI CTCGAG 1 cut(s) 212
XspI CTAG 2 cut(s) 674, 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.