pycom04g10980

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0008470
Physical Location & Seq
Forward (+)
13791227 .. 13793356
2130 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g10980.2

Sequence Viewer

Length: 636 bp
ATGAACTCCTTCACCAACACCACCAATTTCGACAACCTCATGCTCCAAACCCTAATGTGCAGGCTCCAAATCCGCCCCCCCACCAACAACTCCTTCCTCTCCCAAACCCTCGAGGATCTCCTCCTCGACGCTGCCAATCTCACCGCCGACGACGACGATGACGACGAGAACAAGACCCAGCTCGCCAAAGAAGAATCCCGCCTCGAGAAGGATATCATCCGGGTTATTCTCAGCGGCAAGACCGATTCCCTCAAGCCCAATTCTGGTCAGGCCGTCACCATTGGAGAGCATCACATTTGCGTGGGGTTTCATGAGGAATCGGGTTCGGACTATCGAGTTTGGGAGTGGCATGGGCACATCATGCTCTTTGATGAAGAGAACGGGTACACCCCTGAGTATATATATGGGAATTACTTTGAGAGGTTGATTGGTAAGGCTCGTGGAAGTGGTGGCAGCGGAGGTGGCAGTGTTCTTGTTGAGGAGGCCAAGGAGGAAGAGGAAGAGGAAGAGGAGGAGGAGGAGGATAAAGAGAAAGTTATTACTTTGGGGCTCAGGGAGTTGATTGATGGCGGGGATTCGGGTCAGGGTCGAATTCTTCATCGGAATATCAATGCGGGTTCTACAAGGATTACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

212

Amino Acids

23.67

Weight (kDa)

4.47

Isoelectric Point (pI)

46.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 110
AciI CCGC 7 cut(s) 73, 144, 199, 234, 456, 570, 614
AclWI GGATC 1 cut(s) 123
AcsI RAATTY 1 cut(s) 591
AfaI GTAC 1 cut(s) 386
AfiI CCNNNNNNNGG 2 cut(s) 208, 263
AjuI GAANNNNNNNTTGG 2 cut(s) 77, 109
AluBI AGCT 1 cut(s) 181
AluI AGCT 1 cut(s) 181
AlwI GGATC 1 cut(s) 123
Ama87I CYCGRG 2 cut(s) 110, 203
AoxI GGCC 2 cut(s) 270, 483
ApeKI GCWGC 2 cut(s) 131, 453
ApoI RAATTY 1 cut(s) 591
Asp700I GAANNNNTTC 1 cut(s) 8
AsuC2I CCSGG 1 cut(s) 221
AsuHPI GGTGA 3 cut(s) 4, 133, 268
AvaI CYCGRG 2 cut(s) 110, 203
BaeGI GKGCMC 1 cut(s) 357
BanII GRGCYC 1 cut(s) 552
BauI CACGAG 1 cut(s) 438
BbvI GCAGC 2 cut(s) 118, 465
BccI CCATC 1 cut(s) 560
BceAI ACGGC 1 cut(s) 257
BcnI CCSGG 1 cut(s) 221
BisI GCNGC 3 cut(s) 132, 235, 454
BlsI GCNGC 3 cut(s) 133, 236, 455
Bme1390I CCNGG 1 cut(s) 221
BmeT110I CYCGRG 2 cut(s) 110, 203
BmiI GGNNCC 1 cut(s) 65
BmrFI CCNGG 1 cut(s) 221
BmsI GCATC 1 cut(s) 298
Bpu10I CCTNAGC 1 cut(s) 551
BpuEI CTTGAG 1 cut(s) 236
BpuMI CCSGG 1 cut(s) 221
BsaJI CCNNGG 1 cut(s) 486
Bsc4I CCNNNNNNNGG 2 cut(s) 208, 263
BseDI CCNNGG 1 cut(s) 486
BseGI GGATG 1 cut(s) 216
BseLI CCNNNNNNNGG 2 cut(s) 208, 263
BseMII CTCAG 3 cut(s) 244, 384, 565
BseRI GAGGAG 7 cut(s) 110, 113, 494, 524, 527, 530, 533
BseSI GKGCMC 1 cut(s) 357
BseXI GCAGC 2 cut(s) 118, 465
BseYI CCCAGC 1 cut(s) 177
BsgI GTGCAG 1 cut(s) 79
BshFI GGCC 2 cut(s) 272, 485
BsiHKCI CYCGRG 2 cut(s) 110, 203
BsiSI CCGG 1 cut(s) 220
BslI CCNNNNNNNGG 2 cut(s) 208, 263
BsnI GGCC 2 cut(s) 272, 485
BsoBI CYCGRG 2 cut(s) 110, 203
Bsp1286I GDGCHC 2 cut(s) 357, 552
Bsp143I GATC 1 cut(s) 115
BspACI CCGC 7 cut(s) 73, 144, 199, 234, 456, 570, 614
BspANI GGCC 2 cut(s) 272, 485
BspCNI CTCAG 3 cut(s) 243, 385, 564
BspHI TCATGA 1 cut(s) 310
BspLI GGNNCC 1 cut(s) 65
BspPI GGATC 1 cut(s) 123
BssECI CCNNGG 1 cut(s) 486
BssMI GATC 1 cut(s) 115
BssSI CACGAG 1 cut(s) 438
BssT1I CCWWGG 1 cut(s) 486
Bst2BI CACGAG 1 cut(s) 438
Bst6I CTCTTC 4 cut(s) 369, 489, 495, 501
BstAPI GCANNNNNTGC 1 cut(s) 361
BstC8I GCNNGC 2 cut(s) 62, 183
BstDEI CTNAG 3 cut(s) 230, 393, 551
BstENI CCTNNNNNAGG 1 cut(s) 206
BstF5I GGATG 1 cut(s) 216
BstKTI GATC 1 cut(s) 118
BstMBI GATC 1 cut(s) 115
BstMWI GCNNNNNNNGC 2 cut(s) 361, 462
BstSCI CCNGG 1 cut(s) 219
BstSLI GKGCMC 1 cut(s) 357
BstV1I GCAGC 2 cut(s) 118, 465
BstX2I RGATCY 1 cut(s) 115
BstYI RGATCY 1 cut(s) 115
BsuRI GGCC 2 cut(s) 272, 485
BtsCI GGATG 1 cut(s) 216
BtsI GCAGTG 1 cut(s) 472
BtsIMutI CAGTG 1 cut(s) 472
Cac8I GCNNGC 2 cut(s) 62, 183
CciI TCATGA 1 cut(s) 310
CseI GACGC 1 cut(s) 137
Csp6I GTAC 1 cut(s) 385
CviAII CATG 4 cut(s) 40, 311, 350, 361
CviJI RGCY 7 cut(s) 64, 181, 256, 272, 437, 485, 550
CviKI_1 RGCY 7 cut(s) 64, 181, 256, 272, 437, 485, 550
CviQI GTAC 1 cut(s) 385
DdeI CTNAG 3 cut(s) 230, 393, 551
DpnI GATC 1 cut(s) 117
DpnII GATC 1 cut(s) 115
Eam1104I CTCTTC 4 cut(s) 369, 489, 495, 501
EarI CTCTTC 4 cut(s) 369, 489, 495, 501
EciI GGCGGA 1 cut(s) 62
Eco130I CCWWGG 1 cut(s) 486
Eco24I GRGCYC 1 cut(s) 552
Eco32I GATATC 1 cut(s) 214
Eco88I CYCGRG 2 cut(s) 110, 203
EcoNI CCTNNNNNAGG 1 cut(s) 206
EcoRI GAATTC 1 cut(s) 591
EcoRV GATATC 1 cut(s) 214
EcoT14I CCWWGG 1 cut(s) 486
EcoT38I GRGCYC 1 cut(s) 552
ErhI CCWWGG 1 cut(s) 486
FaeI CATG 4 cut(s) 43, 314, 353, 364
FaiI YATR 8 cut(s) 41, 312, 351, 362, 399, 401, 403, 405
FatI CATG 4 cut(s) 39, 310, 349, 360
FauI CCCGC 3 cut(s) 206, 563, 607
Fnu4HI GCNGC 3 cut(s) 132, 235, 454
FokI GGATG 1 cut(s) 203
FriOI GRGCYC 1 cut(s) 552
Fsp4HI GCNGC 3 cut(s) 132, 235, 454
GluI GCNGC 3 cut(s) 132, 235, 454
GsaI CCCAGC 1 cut(s) 181
HaeIII GGCC 2 cut(s) 272, 485
HapII CCGG 1 cut(s) 220
HgaI GACGC 1 cut(s) 137
Hin1II CATG 4 cut(s) 43, 314, 353, 364
HinfI GANTC 4 cut(s) 194, 245, 317, 575
HpaII CCGG 1 cut(s) 220
HphI GGTGA 3 cut(s) 4, 133, 268
Hpy166II GTNNAC 1 cut(s) 387
Hpy188I TCNGA 2 cut(s) 328, 603
Hpy188III TCNNGA 2 cut(s) 205, 311
Hpy8I GTNNAC 1 cut(s) 387
Hpy99I CGWCG 5 cut(s) 131, 152, 155, 158, 167
HpyAV CCTTC 3 cut(s) 19, 103, 202
HpyCH4V TGCA 1 cut(s) 60
HpyF10VI GCNNNNNNNGC 2 cut(s) 361, 462
HpyF3I CTNAG 3 cut(s) 230, 393, 551
Hsp92II CATG 4 cut(s) 43, 314, 353, 364
Kzo9I GATC 1 cut(s) 115
LmnI GCTCC 2 cut(s) 48, 69
LpnPI CCDG 8 cut(s) 46, 191, 233, 249, 254, 405, 538, 569
Lsp1109I GCAGC 2 cut(s) 118, 465
LweI GCATC 1 cut(s) 298
MaeIII GTNAC 1 cut(s) 274
MalI GATC 1 cut(s) 117
MboI GATC 1 cut(s) 115
MboII GAAGA 6 cut(s) 203, 386, 506, 512, 518, 587
MflI RGATCY 1 cut(s) 115
MhlI GDGCHC 2 cut(s) 357, 552
MluCI AATT 4 cut(s) 25, 259, 409, 591
MroXI GAANNNNTTC 1 cut(s) 8
MslI CAYNNNNRTG 1 cut(s) 299
MspA1I CMGCKG 2 cut(s) 234, 456
MspI CCGG 1 cut(s) 220
MspR9I CCNGG 1 cut(s) 221
MwoI GCNNNNNNNGC 2 cut(s) 361, 462
NciI CCSGG 1 cut(s) 221
NdeII GATC 1 cut(s) 115
NlaIII CATG 4 cut(s) 43, 314, 353, 364
NlaIV GGNNCC 1 cut(s) 65
NmuCI GTSAC 1 cut(s) 274
PaeR7I CTCGAG 2 cut(s) 110, 203
PagI TCATGA 1 cut(s) 310
PcsI WCGNNNNNNNCGW 2 cut(s) 159, 162
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 4 cut(s) 194, 245, 317, 575
PkrI GCNGC 3 cut(s) 133, 236, 455
PspFI CCCAGC 1 cut(s) 177
PspN4I GGNNCC 1 cut(s) 65
PspXI VCTCGAGB 1 cut(s) 110
PsuI RGATCY 1 cut(s) 115
RsaI GTAC 1 cut(s) 386
RsaNI GTAC 1 cut(s) 385
RseI CAYNNNNRTG 1 cut(s) 299
SatI GCNGC 3 cut(s) 132, 235, 454
Sau3AI GATC 1 cut(s) 115
ScrFI CCNGG 1 cut(s) 221
SduI GDGCHC 2 cut(s) 357, 552
SetI ASST 5 cut(s) 39, 183, 425, 463, 635
SfaNI GCATC 1 cut(s) 298
Sfr274I CTCGAG 2 cut(s) 110, 203
SlaI CTCGAG 2 cut(s) 110, 203
SmiMI CAYNNNNRTG 1 cut(s) 299
SmlI CTYRAG 3 cut(s) 110, 203, 251
SmoI CTYRAG 3 cut(s) 110, 203, 251
Sse9I AATT 4 cut(s) 25, 259, 409, 591
SsiI CCGC 7 cut(s) 73, 144, 199, 234, 456, 570, 614
StyD4I CCNGG 1 cut(s) 219
StyI CCWWGG 1 cut(s) 486
TaqI TCGA 6 cut(s) 30, 111, 126, 204, 334, 589
TaqII GACCGA 1 cut(s) 257
TasI AATT 4 cut(s) 25, 259, 409, 591
TauI GCSGC 1 cut(s) 237
TfiI GAWTC 4 cut(s) 194, 245, 317, 575
TscAI CASTG 1 cut(s) 472
TseFI GTSAC 1 cut(s) 274
TseI GCWGC 2 cut(s) 131, 453
Tsp45I GTSAC 1 cut(s) 274
TspDTI ATGAA 4 cut(s) 17, 299, 387, 587
TspRI CASTG 1 cut(s) 472
XagI CCTNNNNNAGG 1 cut(s) 206
XapI RAATTY 1 cut(s) 591
XhoI CTCGAG 2 cut(s) 110, 203
XmnI GAANNNNTTC 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.