MD12G1138000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0008470
Physical Location & Seq
Forward (+)
21565899 .. 21568833
2935 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1138000.v1.1.491

Sequence Viewer

Length: 603 bp
ATGAGCTCCTTCACCAACACCACCAATTTCGACAACCTCATGCTCCAAACCCTAATGGGCAGGCTCCAAATCCGACCCCCCACCAACAACTCCTTCCTCTCCCAAACCCTCGAGGAGCTCCTCTTCGACGCCGCCAATCTCTCCGACGACGACGACGATGAGAACAAGACCCAGCTCGCCAAGGAAGAATCCCGCCTCGAGAAGGAGATCATCCGGGTTGTTCTCAGCGGCAAGACCGATTCCCTCAAGCCCAATTCGGGTCAGGCCGTCACGATCGGGGAGCACCACATTTGCGTTGGGTTTCATGAGGAATCGGGTTCGGACTATCGGGTTTGGGAGTGGCATGGGCATATCATGCTCTTCGATGAAGAGAACGGGTACACCCCTGAGTATATATATGGGAATTACTTTGAGAGGCTGATTGGTAAGGCTCGTGGAAGTGGCGGCGGCGGAAGTGGTGGTGTTCGTGAAGAGGAGACCAAGGAGGAAGATAAAGAGAAGGTGACCACTTTGGGGCTCAGGGAGTTGATTGATGGTGTGGATTCGGGTCAGGGTCGGATTCTTCATCGGAATATCAATGCGGGTTCTACAAGGATTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

201

Amino Acids

22.4

Weight (kDa)

4.83

Isoelectric Point (pI)

39.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 110
AciI CCGC 7 cut(s) 132, 193, 228, 444, 447, 450, 581
AcyI GRCGYC 1 cut(s) 129
AfaI GTAC 1 cut(s) 380
AfiI CCNNNNNNNGG 3 cut(s) 202, 257, 513
AjuI GAANNNNNNNTTGG 2 cut(s) 77, 109
AluBI AGCT 3 cut(s) 6, 118, 175
AluI AGCT 3 cut(s) 6, 118, 175
Alw21I GWGCWC 3 cut(s) 8, 120, 285
Alw26I GTCTC 1 cut(s) 470
Ama87I CYCGRG 2 cut(s) 110, 197
AoxI GGCC 1 cut(s) 264
AseI ATTAAT 1 cut(s) 597
AsuC2I CCSGG 1 cut(s) 215
AsuHPI GGTGA 2 cut(s) 4, 514
AvaI CYCGRG 2 cut(s) 110, 197
BanII GRGCYC 3 cut(s) 8, 120, 519
BauI CACGAG 1 cut(s) 432
Bbv12I GWGCWC 3 cut(s) 8, 120, 285
BccI CCATC 1 cut(s) 527
BceAI ACGGC 1 cut(s) 251
BcnI CCSGG 1 cut(s) 215
BcoDI GTCTC 1 cut(s) 470
BisI GCNGC 4 cut(s) 132, 229, 445, 448
BlsI GCNGC 4 cut(s) 133, 230, 446, 449
Bme1390I CCNGG 1 cut(s) 215
BmeT110I CYCGRG 2 cut(s) 110, 197
BmiI GGNNCC 1 cut(s) 65
BmrFI CCNGG 1 cut(s) 215
Bpu10I CCTNAGC 1 cut(s) 518
BpuEI CTTGAG 1 cut(s) 230
BpuMI CCSGG 1 cut(s) 215
BsaHI GRCGYC 1 cut(s) 129
BsaI GGTCTC 1 cut(s) 470
BsaJI CCNNGG 2 cut(s) 180, 480
Bsc4I CCNNNNNNNGG 3 cut(s) 202, 257, 513
BseDI CCNNGG 2 cut(s) 180, 480
BseGI GGATG 1 cut(s) 210
BseLI CCNNNNNNNGG 3 cut(s) 202, 257, 513
BseMII CTCAG 3 cut(s) 238, 378, 532
BseRI GAGGAG 3 cut(s) 110, 128, 488
BseYI CCCAGC 1 cut(s) 171
Bsh1285I CGRYCG 1 cut(s) 276
BshFI GGCC 1 cut(s) 266
BsiEI CGRYCG 1 cut(s) 276
BsiHKAI GWGCWC 3 cut(s) 8, 120, 285
BsiHKCI CYCGRG 2 cut(s) 110, 197
BsiSI CCGG 1 cut(s) 214
BslI CCNNNNNNNGG 3 cut(s) 202, 257, 513
BsmAI GTCTC 1 cut(s) 470
BsnI GGCC 1 cut(s) 266
Bso31I GGTCTC 1 cut(s) 470
BsoBI CYCGRG 2 cut(s) 110, 197
Bsp1286I GDGCHC 4 cut(s) 8, 120, 285, 519
Bsp143I GATC 2 cut(s) 207, 273
BspACI CCGC 7 cut(s) 132, 193, 228, 444, 447, 450, 581
BspANI GGCC 1 cut(s) 266
BspCNI CTCAG 3 cut(s) 237, 379, 531
BspHI TCATGA 1 cut(s) 304
BspLI GGNNCC 1 cut(s) 65
BspQI GCTCTTC 1 cut(s) 365
BspTNI GGTCTC 1 cut(s) 470
BssECI CCNNGG 2 cut(s) 180, 480
BssMI GATC 2 cut(s) 207, 273
BssNI GRCGYC 1 cut(s) 129
BssSI CACGAG 1 cut(s) 432
BssT1I CCWWGG 2 cut(s) 180, 480
Bst2BI CACGAG 1 cut(s) 432
Bst6I CTCTTC 4 cut(s) 128, 363, 365, 465
BstACI GRCGYC 1 cut(s) 129
BstAPI GCANNNNNTGC 1 cut(s) 355
BstC8I GCNNGC 2 cut(s) 62, 177
BstDEI CTNAG 3 cut(s) 224, 387, 518
BstEII GGTNACC 1 cut(s) 502
BstENI CCTNNNNNAGG 1 cut(s) 200
BstF5I GGATG 1 cut(s) 210
BstKTI GATC 2 cut(s) 210, 276
BstMAI GTCTC 1 cut(s) 470
BstMBI GATC 2 cut(s) 207, 273
BstMCI CGRYCG 1 cut(s) 276
BstMWI GCNNNNNNNGC 1 cut(s) 355
BstPI GGTNACC 1 cut(s) 502
BstSCI CCNGG 1 cut(s) 213
BsuRI GGCC 1 cut(s) 266
BtsCI GGATG 1 cut(s) 210
Cac8I GCNNGC 2 cut(s) 62, 177
CciI TCATGA 1 cut(s) 304
CseI GACGC 1 cut(s) 137
Csp6I GTAC 1 cut(s) 379
CviAII CATG 4 cut(s) 40, 305, 344, 355
CviJI RGCY 9 cut(s) 6, 64, 118, 175, 250, 266, 418, 431, 517
CviKI_1 RGCY 9 cut(s) 6, 64, 118, 175, 250, 266, 418, 431, 517
CviQI GTAC 1 cut(s) 379
DdeI CTNAG 3 cut(s) 224, 387, 518
DpnI GATC 2 cut(s) 209, 275
DpnII GATC 2 cut(s) 207, 273
Eam1104I CTCTTC 4 cut(s) 128, 363, 365, 465
EarI CTCTTC 4 cut(s) 128, 363, 365, 465
EciI GGCGGA 1 cut(s) 465
Ecl136II GAGCTC 2 cut(s) 6, 118
Eco130I CCWWGG 2 cut(s) 180, 480
Eco24I GRGCYC 3 cut(s) 8, 120, 519
Eco31I GGTCTC 1 cut(s) 470
Eco53kI GAGCTC 2 cut(s) 6, 118
Eco88I CYCGRG 2 cut(s) 110, 197
Eco91I GGTNACC 1 cut(s) 502
EcoICRI GAGCTC 2 cut(s) 6, 118
EcoNI CCTNNNNNAGG 1 cut(s) 200
EcoO65I GGTNACC 1 cut(s) 502
EcoT14I CCWWGG 2 cut(s) 180, 480
EcoT38I GRGCYC 3 cut(s) 8, 120, 519
ErhI CCWWGG 2 cut(s) 180, 480
FaeI CATG 4 cut(s) 43, 308, 347, 358
FaiI YATR 9 cut(s) 41, 306, 345, 351, 356, 393, 395, 397, 399
FatI CATG 4 cut(s) 39, 304, 343, 354
FauI CCCGC 2 cut(s) 200, 574
Fnu4HI GCNGC 4 cut(s) 132, 229, 445, 448
FokI GGATG 1 cut(s) 197
FriOI GRGCYC 3 cut(s) 8, 120, 519
Fsp4HI GCNGC 4 cut(s) 132, 229, 445, 448
GluI GCNGC 4 cut(s) 132, 229, 445, 448
GsaI CCCAGC 1 cut(s) 175
HaeIII GGCC 1 cut(s) 266
HapII CCGG 1 cut(s) 214
HgaI GACGC 1 cut(s) 137
Hin1I GRCGYC 1 cut(s) 129
Hin1II CATG 4 cut(s) 43, 308, 347, 358
HinfI GANTC 5 cut(s) 188, 239, 311, 542, 559
HpaII CCGG 1 cut(s) 214
HphI GGTGA 2 cut(s) 4, 514
Hpy166II GTNNAC 1 cut(s) 381
Hpy188I TCNGA 5 cut(s) 74, 145, 322, 558, 570
Hpy188III TCNNGA 4 cut(s) 199, 271, 305, 467
Hpy8I GTNNAC 1 cut(s) 381
Hpy99I CGWCG 5 cut(s) 131, 149, 152, 155, 158
HpyAV CCTTC 4 cut(s) 19, 103, 196, 493
HpyF10VI GCNNNNNNNGC 1 cut(s) 355
HpyF3I CTNAG 3 cut(s) 224, 387, 518
Hsp92I GRCGYC 1 cut(s) 129
Hsp92II CATG 4 cut(s) 43, 308, 347, 358
Kzo9I GATC 2 cut(s) 207, 273
LguI GCTCTTC 1 cut(s) 365
LmnI GCTCC 6 cut(s) 11, 48, 69, 115, 123, 280
LpnPI CCDG 7 cut(s) 46, 185, 227, 248, 399, 505, 536
MaeIII GTNAC 2 cut(s) 268, 502
MalI GATC 2 cut(s) 209, 275
MboI GATC 2 cut(s) 207, 273
MboII GAAGA 7 cut(s) 115, 197, 352, 380, 482, 500, 554
MhlI GDGCHC 4 cut(s) 8, 120, 285, 519
MluCI AATT 4 cut(s) 25, 253, 403, 598
MmeI TCCRAC 3 cut(s) 97, 168, 536
MseI TTAA 2 cut(s) 597, 601
MspA1I CMGCKG 1 cut(s) 228
MspI CCGG 1 cut(s) 214
MspR9I CCNGG 1 cut(s) 215
MwoI GCNNNNNNNGC 1 cut(s) 355
NciI CCSGG 1 cut(s) 215
NdeII GATC 2 cut(s) 207, 273
NlaIII CATG 4 cut(s) 43, 308, 347, 358
NlaIV GGNNCC 1 cut(s) 65
NmuCI GTSAC 2 cut(s) 268, 502
PacI TTAATTAA 1 cut(s) 601
PaeR7I CTCGAG 2 cut(s) 110, 197
PagI TCATGA 1 cut(s) 304
PciSI GCTCTTC 1 cut(s) 365
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PfeI GAWTC 5 cut(s) 188, 239, 311, 542, 559
PkrI GCNGC 4 cut(s) 133, 230, 446, 449
Ple19I CGATCG 1 cut(s) 276
PshBI ATTAAT 1 cut(s) 597
Psp124BI GAGCTC 2 cut(s) 8, 120
PspEI GGTNACC 1 cut(s) 502
PspFI CCCAGC 1 cut(s) 171
PspN4I GGNNCC 1 cut(s) 65
PspXI VCTCGAGB 1 cut(s) 110
PvuI CGATCG 1 cut(s) 276
RsaI GTAC 1 cut(s) 380
RsaNI GTAC 1 cut(s) 379
SacI GAGCTC 2 cut(s) 8, 120
SapI GCTCTTC 1 cut(s) 365
SaqAI TTAA 2 cut(s) 597, 601
SatI GCNGC 4 cut(s) 132, 229, 445, 448
Sau3AI GATC 2 cut(s) 207, 273
ScrFI CCNGG 1 cut(s) 215
SduI GDGCHC 4 cut(s) 8, 120, 285, 519
SetI ASST 5 cut(s) 8, 39, 120, 177, 504
Sfr274I CTCGAG 2 cut(s) 110, 197
SlaI CTCGAG 2 cut(s) 110, 197
SmlI CTYRAG 3 cut(s) 110, 197, 245
SmoI CTYRAG 3 cut(s) 110, 197, 245
Sse9I AATT 4 cut(s) 25, 253, 403, 598
SsiI CCGC 7 cut(s) 132, 193, 228, 444, 447, 450, 581
SstI GAGCTC 2 cut(s) 8, 120
StyD4I CCNGG 1 cut(s) 213
StyI CCWWGG 2 cut(s) 180, 480
TaqI TCGA 5 cut(s) 30, 111, 126, 198, 363
TaqII GACCGA 1 cut(s) 251
TasI AATT 4 cut(s) 25, 253, 403, 598
TauI GCSGC 4 cut(s) 134, 231, 447, 450
TfiI GAWTC 5 cut(s) 188, 239, 311, 542, 559
Tru1I TTAA 2 cut(s) 597, 601
Tru9I TTAA 2 cut(s) 597, 601
TseFI GTSAC 2 cut(s) 268, 502
Tsp45I GTSAC 2 cut(s) 268, 502
TspDTI ATGAA 3 cut(s) 293, 381, 554
VspI ATTAAT 1 cut(s) 597
XagI CCTNNNNNAGG 1 cut(s) 200
XcmI CCANNNNNNNNNTGG 1 cut(s) 293
XhoI CTCGAG 2 cut(s) 110, 197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.