AT3G54480

F-box-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0007291
Physical Location & Seq
Reverse (-)
20170654 .. 20173530
2877 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G54480.1

Sequence Viewer

Length: 825 bp
ATGGAGTTACATGAGATCTCAAAGCTCGACAATAAGAAGCATCCAGAGAGAATTGTTGAATCGAAGATGAAGAAGAGAATGAAACCTTATTCTCTCACGTCTCTTAACAATCTCGACGATGGTTGCCTTATGCATATCCTCAGCTTCCTATCTCCAATTCCAGATAGGTATAACACAGCACTCGTTTGCCACAGATGGAGATATCTGGCGTGTCACCCTCGCTTGTGGCTCCGTGTTGACCGTTTTGTCAAGGATTTATCTCAGCCTGGAGTTTTCCTCAACATTGAGTCAGCTGTATCTGCAGCAAGACCCGGCGATACCATTTTAATAGTAGCCGGTGGGAACTATCGCGTTTCTAATATTCAGATTAAAAAGCCTCTTTGCCTGGTCGGTGGAGGCGAGATTCCTGATGAAACCACCCTCGTCTGTGCTCGTGGTTCAGACAGTGCCCTGGAACTGTTATCAACCTGCAAGCTTGCGAATTTAACAGTAAAAGCAGAGCTCGGGTGCTGTTTGCTTCATAGGAGTGGAAGGCTAACGATTGATGGATGTGTGCTTCAATGTGAGACAAACCCTTTGGATCATCTCTCATGCCCAATCGTGAGCACTGCTGGAGATGAAGACATTGAAAATATCTTAAGCCATGTGGAAGTGAAAGAAACTGTTACTGGAAAGATTAAAGCAAACAGTGTAACCGTTTTGCAGACAAGAATCGAGGGAGGTGCAAAGGCTGTTTCAACAAGGGGAGACTTGGTTCTGCAGCGTGTTCGAGTCATGTATTCCAAGGCTTACCTCTATTTCTGGTTTGATGTAGATTATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

274

Amino Acids

30.56

Weight (kDa)

7.97

Isoelectric Point (pI)

44.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 35 - 77 1.1e-06 F-box domain
F-box-like PF12937 43 - 80 1.3e-09 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 245
Acc36I ACCTGC 1 cut(s) 476
AccII CGCG 1 cut(s) 351
AclWI GGATC 1 cut(s) 588
AcsI RAATTY 1 cut(s) 481
AflII CTTAAG 1 cut(s) 637
AgsI TTSAA 4 cut(s) 59, 560, 629, 738
AjiI CACGTC 1 cut(s) 99
AjnI CCWGG 3 cut(s) 265, 384, 450
AloI GAACNNNNNNTCC 2 cut(s) 738, 770
AluBI AGCT 5 cut(s) 25, 144, 293, 475, 502
AluI AGCT 5 cut(s) 25, 144, 293, 475, 502
Alw21I GWGCWC 3 cut(s) 433, 504, 608
Alw26I GTCTC 3 cut(s) 105, 560, 741
AlwI GGATC 1 cut(s) 588
Ama87I CYCGRG 1 cut(s) 503
ApeKI GCWGC 2 cut(s) 302, 760
ApoI RAATTY 1 cut(s) 481
ArsI GACNNNNNNTTYG 2 cut(s) 559, 591
AsuC2I CCSGG 1 cut(s) 312
AsuHPI GGTGA 1 cut(s) 206
AvaI CYCGRG 1 cut(s) 503
BaeGI GKGCMC 1 cut(s) 451
BanII GRGCYC 1 cut(s) 504
BauI CACGAG 1 cut(s) 432
BbsI GAAGAC 1 cut(s) 627
Bbv12I GWGCWC 3 cut(s) 433, 504, 608
BbvCI CCTCAGC 1 cut(s) 140
BbvI GCAGC 2 cut(s) 314, 772
BccI CCATC 3 cut(s) 113, 189, 539
BcgI CGANNNNNNTGC 4 cut(s) 704, 738, 749, 783
BciT130I CCWGG 3 cut(s) 267, 386, 452
BcnI CCSGG 1 cut(s) 312
BcoDI GTCTC 3 cut(s) 105, 560, 741
BfmI CTRYAG 2 cut(s) 300, 758
BfrI CTTAAG 1 cut(s) 637
BfuAI ACCTGC 1 cut(s) 476
BglII AGATCT 1 cut(s) 15
BisI GCNGC 2 cut(s) 303, 761
BlsI GCNGC 2 cut(s) 304, 762
Bme1390I CCNGG 4 cut(s) 267, 312, 386, 452
BmeT110I CYCGRG 1 cut(s) 503
BmgBI CACGTC 1 cut(s) 99
BmiI GGNNCC 1 cut(s) 230
BmrFI CCNGG 4 cut(s) 267, 312, 386, 452
BmsI GCATC 1 cut(s) 49
BpiI GAAGAC 1 cut(s) 627
BplI GAGNNNNNCTC 2 cut(s) 261, 293
BpmI CTGGAG 2 cut(s) 288, 633
Bpu10I CCTNAGC 1 cut(s) 140
BpuMI CCSGG 1 cut(s) 312
BsaJI CCNNGG 2 cut(s) 450, 783
BsaXI ACNNNNNCTCC 2 cut(s) 738, 768
Bse118I RCCGGY 1 cut(s) 335
Bse1I ACTGG 1 cut(s) 673
BseBI CCWGG 3 cut(s) 267, 386, 452
BseDI CCNNGG 2 cut(s) 450, 783
BseGI GGATG 2 cut(s) 40, 554
BseMII CTCAG 2 cut(s) 154, 275
BseNI ACTGG 1 cut(s) 673
BseSI GKGCMC 1 cut(s) 451
BseXI GCAGC 2 cut(s) 314, 772
Bsh1236I CGCG 1 cut(s) 351
BsiHKAI GWGCWC 3 cut(s) 433, 504, 608
BsiHKCI CYCGRG 1 cut(s) 503
BsiSI CCGG 2 cut(s) 312, 336
BsmAI GTCTC 3 cut(s) 105, 560, 741
BsmBI CGTCTC 1 cut(s) 105
BsoBI CYCGRG 1 cut(s) 503
Bsp1286I GDGCHC 4 cut(s) 433, 451, 504, 608
Bsp143I GATC 2 cut(s) 15, 580
BspCNI CTCAG 2 cut(s) 153, 274
BspFNI CGCG 1 cut(s) 351
BspLI GGNNCC 1 cut(s) 230
BspMAI CTGCAG 2 cut(s) 304, 762
BspMI ACCTGC 1 cut(s) 476
BspPI GGATC 1 cut(s) 588
BspTI CTTAAG 1 cut(s) 637
BsrFI RCCGGY 1 cut(s) 335
BsrI ACTGG 1 cut(s) 673
BssAI RCCGGY 1 cut(s) 335
BssECI CCNNGG 2 cut(s) 450, 783
BssMI GATC 2 cut(s) 15, 580
BssSI CACGAG 1 cut(s) 432
BssT1I CCWWGG 1 cut(s) 783
Bst2BI CACGAG 1 cut(s) 432
Bst2UI CCWGG 3 cut(s) 267, 386, 452
Bst4CI ACNGT 7 cut(s) 242, 446, 459, 490, 664, 689, 697
Bst6I CTCTTC 1 cut(s) 68
BstAFI CTTAAG 1 cut(s) 637
BstC8I GCNNGC 2 cut(s) 473, 477
BstDEI CTNAG 2 cut(s) 140, 261
BstF5I GGATG 2 cut(s) 40, 554
BstFNI CGCG 1 cut(s) 351
BstKTI GATC 2 cut(s) 18, 583
BstMAI GTCTC 3 cut(s) 105, 560, 741
BstMBI GATC 2 cut(s) 15, 580
BstMWI GCNNNNNNNGC 1 cut(s) 299
BstNI CCWGG 3 cut(s) 267, 386, 452
BstSCI CCNGG 4 cut(s) 265, 310, 384, 450
BstSFI CTRYAG 2 cut(s) 300, 758
BstSLI GKGCMC 1 cut(s) 451
BstUI CGCG 1 cut(s) 351
BstV1I GCAGC 2 cut(s) 314, 772
BstV2I GAAGAC 1 cut(s) 627
BstX2I RGATCY 1 cut(s) 15
BstYI RGATCY 1 cut(s) 15
BtrI CACGTC 1 cut(s) 99
BtsCI GGATG 2 cut(s) 40, 554
BtsI GCAGTG 1 cut(s) 606
BtsIMutI CAGTG 3 cut(s) 451, 606, 694
BveI ACCTGC 1 cut(s) 476
Cac8I GCNNGC 2 cut(s) 473, 477
Cfr10I RCCGGY 1 cut(s) 335
CviAII CATG 4 cut(s) 11, 591, 644, 775
DdeI CTNAG 2 cut(s) 140, 261
DpnI GATC 2 cut(s) 17, 582
DpnII GATC 2 cut(s) 15, 580
DrdI GACNNNNNNGTC 1 cut(s) 245
DseDI GACNNNNNNGTC 1 cut(s) 245
Eam1104I CTCTTC 1 cut(s) 68
EarI CTCTTC 1 cut(s) 68
Ecl136II GAGCTC 1 cut(s) 502
Eco130I CCWWGG 1 cut(s) 783
Eco24I GRGCYC 1 cut(s) 504
Eco32I GATATC 1 cut(s) 203
Eco53kI GAGCTC 1 cut(s) 502
Eco88I CYCGRG 1 cut(s) 503
EcoICRI GAGCTC 1 cut(s) 502
EcoRII CCWGG 3 cut(s) 265, 384, 450
EcoRV GATATC 1 cut(s) 203
EcoT14I CCWWGG 1 cut(s) 783
EcoT22I ATGCAT 1 cut(s) 135
EcoT38I GRGCYC 1 cut(s) 504
ErhI CCWWGG 1 cut(s) 783
Esp3I CGTCTC 1 cut(s) 105
FaeI CATG 4 cut(s) 14, 594, 647, 778
FaiI YATR 9 cut(s) 12, 131, 135, 171, 522, 592, 645, 776, 819
FatI CATG 4 cut(s) 10, 590, 643, 774
Fnu4HI GCNGC 2 cut(s) 303, 761
FokI GGATG 2 cut(s) 27, 561
FriOI GRGCYC 1 cut(s) 504
Fsp4HI GCNGC 2 cut(s) 303, 761
GluI GCNGC 2 cut(s) 303, 761
GsuI CTGGAG 2 cut(s) 288, 633
HapII CCGG 2 cut(s) 312, 336
Hin1II CATG 4 cut(s) 14, 594, 647, 778
HincII GTYRAC 1 cut(s) 238
HindII GTYRAC 1 cut(s) 238
HindIII AAGCTT 1 cut(s) 473
HinfI GANTC 5 cut(s) 59, 287, 403, 711, 771
HpaII CCGG 2 cut(s) 312, 336
HphI GGTGA 1 cut(s) 206
Hpy166II GTNNAC 1 cut(s) 238
Hpy188I TCNGA 2 cut(s) 366, 442
Hpy188III TCNNGA 5 cut(s) 44, 113, 161, 407, 601
Hpy8I GTNNAC 1 cut(s) 238
Hpy99I CGWCG 1 cut(s) 119
HpyAV CCTTC 1 cut(s) 525
HpyCH4III ACNGT 7 cut(s) 242, 446, 459, 490, 664, 689, 697
HpyCH4IV ACGT 1 cut(s) 98
HpyCH4V TGCA 6 cut(s) 133, 302, 471, 703, 725, 760
HpyF10VI GCNNNNNNNGC 1 cut(s) 299
HpyF3I CTNAG 2 cut(s) 140, 261
HpySE526I ACGT 1 cut(s) 98
Hsp92II CATG 4 cut(s) 14, 594, 647, 778
Kzo9I GATC 2 cut(s) 15, 580
LmnI GCTCC 1 cut(s) 234
Lsp1109I GCAGC 2 cut(s) 314, 772
LweI GCATC 1 cut(s) 49
MaeII ACGT 1 cut(s) 98
MaeIII GTNAC 4 cut(s) 6, 212, 664, 691
MalI GATC 2 cut(s) 17, 582
MboI GATC 2 cut(s) 15, 580
MboII GAAGA 4 cut(s) 76, 82, 85, 632
MflI RGATCY 1 cut(s) 15
MhlI GDGCHC 4 cut(s) 433, 451, 504, 608
MluCI AATT 3 cut(s) 51, 156, 481
MlyI GAGTC 2 cut(s) 296, 780
MnlI CCTC 9 cut(s) 149, 228, 287, 387, 389, 431, 709, 713, 803
Mph1103I ATGCAT 1 cut(s) 135
MseI TTAA 6 cut(s) 105, 326, 369, 485, 638, 678
MslI CAYNNNNRTG 1 cut(s) 525
MspA1I CMGCKG 1 cut(s) 293
MspCI CTTAAG 1 cut(s) 637
MspI CCGG 2 cut(s) 312, 336
MspR9I CCNGG 4 cut(s) 267, 312, 386, 452
MvaI CCWGG 3 cut(s) 267, 386, 452
MvnI CGCG 1 cut(s) 351
MwoI GCNNNNNNNGC 1 cut(s) 299
NciI CCSGG 1 cut(s) 312
NdeII GATC 2 cut(s) 15, 580
NlaIII CATG 4 cut(s) 14, 594, 647, 778
NlaIV GGNNCC 1 cut(s) 230
NmuCI GTSAC 1 cut(s) 212
NsiI ATGCAT 1 cut(s) 135
PcsI WCGNNNNNNNCGW 1 cut(s) 396
PfeI GAWTC 3 cut(s) 59, 403, 711
PkrI GCNGC 2 cut(s) 304, 762
PleI GAGTC 2 cut(s) 295, 779
PpsI GAGTC 2 cut(s) 295, 779
Psp124BI GAGCTC 1 cut(s) 504
Psp6I CCWGG 3 cut(s) 265, 384, 450
PspGI CCWGG 3 cut(s) 265, 384, 450
PspN4I GGNNCC 1 cut(s) 230
PstI CTGCAG 2 cut(s) 304, 762
PsuI RGATCY 1 cut(s) 15
PvuII CAGCTG 1 cut(s) 293
RseI CAYNNNNRTG 1 cut(s) 525
SacI GAGCTC 1 cut(s) 504
SaqAI TTAA 6 cut(s) 105, 326, 369, 485, 638, 678
SatI GCNGC 2 cut(s) 303, 761
Sau3AI GATC 2 cut(s) 15, 580
SchI GAGTC 2 cut(s) 296, 780
ScrFI CCNGG 4 cut(s) 267, 312, 386, 452
SduI GDGCHC 4 cut(s) 433, 451, 504, 608
SfaNI GCATC 1 cut(s) 49
SfcI CTRYAG 2 cut(s) 300, 758
SmiMI CAYNNNNRTG 1 cut(s) 525
SmlI CTYRAG 1 cut(s) 637
SmoI CTYRAG 1 cut(s) 637
Sse9I AATT 3 cut(s) 51, 156, 481
SspI AATATT 1 cut(s) 361
SstI GAGCTC 1 cut(s) 504
StyD4I CCNGG 4 cut(s) 265, 310, 384, 450
StyI CCWWGG 1 cut(s) 783
TaaI ACNGT 7 cut(s) 242, 446, 459, 490, 664, 689, 697
TaiI ACGT 1 cut(s) 101
TaqI TCGA 5 cut(s) 27, 62, 114, 714, 769
TasI AATT 3 cut(s) 51, 156, 481
TfiI GAWTC 3 cut(s) 59, 403, 711
Tru1I TTAA 6 cut(s) 105, 326, 369, 485, 638, 678
Tru9I TTAA 6 cut(s) 105, 326, 369, 485, 638, 678
TscAI CASTG 3 cut(s) 451, 613, 694
TseFI GTSAC 1 cut(s) 212
TseI GCWGC 2 cut(s) 302, 760
Tsp45I GTSAC 1 cut(s) 212
TspDTI ATGAA 5 cut(s) 83, 95, 426, 509, 633
TspGWI ACGGA 1 cut(s) 221
TspRI CASTG 3 cut(s) 451, 613, 694
Vha464I CTTAAG 1 cut(s) 637
XapI RAATTY 1 cut(s) 481
Zsp2I ATGCAT 1 cut(s) 135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.