MD02G1290300.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0007291
Physical Location & Seq
Reverse (-)
34614172 .. 34617451
3280 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1290300.v1.1.491

Sequence Viewer

Length: 792 bp
ATGGAGGAGGTGGTGGAGGAGATAGAGCAAAAAAGGTGGAGGAGAAGCCTCACAAAGACAAAGAGAAGCAGGAGCTCCTCAAAGTCGTCCTCATGCCTTATAAATAATCTTGATGATGGATGTCTGATGCACATCTTCAGCTTCCTCTCTCCCATCCCAGATCGGTATAACACCGCCCTCGTTTGCCACAGATGGCTTTACTTGGCATGTCACCCTCGCTTGTGGCTGCGAGTTGACCGGTCTGTCAAAGATCTTTTGGAGCCTGGGGTTTTCCCCAACCTTGAGACAGCTGTTGAGGCTGCAAGGCCAGGCGACACTATTCTAATTGCAGCAGGCGGAAGACATCTTGCTTATAATATTCAAATAAAAAAGCGACTTTGCCTGATTGGTGCAGGTGAGCAACCTGATGAGACGACTATATATTGTTCTCGAGGTTCTGACAGTGCATTGGAGCTCCTGTCGACCTGCAAATTGGCGAACTTGACTGTGAAGGCAGAGCTTGGCTGTTGCTTGCTTCATAGGAGCGGAAGGGTGACGATAGAGGGGTGTGTGCTTCAATGCGAGACGAATCCTCTAGACCATCTTTCGTGCCCTATTGTGAGCACAGCTAGTGCCCAGACGGTAATATCTTCCCCTGTGAAATGTTCTAATGATGGCGTTTCTGTTTTTCGAACTCGAATTGAAGGAGGTGCCAAGGCTGTCTTGACTAGCGGAGACTTGACCTTGCAGCGAGTTCGAGTTATTTATGCTCGGACGTCGATCTTCTTCTGGTTTGACGTAGAGCACCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

264

Amino Acids

29.25

Weight (kDa)

8.41

Isoelectric Point (pI)

67.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 42 - 80 2.4e-09 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 101, 354
AarI CACCTGC 1 cut(s) 383
AasI GACNNNNNNGTC 1 cut(s) 242
AatII GACGTC 1 cut(s) 758
Acc36I ACCTGC 2 cut(s) 383, 473
AccB1I GGYRCC 1 cut(s) 689
AccBSI CCGCTC 1 cut(s) 525
AccI GTMKAC 1 cut(s) 461
AciI CCGC 4 cut(s) 174, 336, 525, 711
AcuI CTGAAG 1 cut(s) 121
AcyI GRCGYC 1 cut(s) 755
AgeI ACCGGT 1 cut(s) 237
AgsI TTSAA 3 cut(s) 362, 557, 683
AjnI CCWGG 2 cut(s) 262, 307
AluBI AGCT 6 cut(s) 75, 141, 290, 454, 499, 608
AluI AGCT 6 cut(s) 75, 141, 290, 454, 499, 608
Alw21I GWGCWC 4 cut(s) 77, 456, 605, 786
Alw26I GTCTC 4 cut(s) 278, 404, 557, 708
Ama87I CYCGRG 1 cut(s) 429
AoxI GGCC 1 cut(s) 305
ApeKI GCWGC 4 cut(s) 226, 299, 329, 727
AsiGI ACCGGT 1 cut(s) 237
AsuHPI GGTGA 3 cut(s) 203, 407, 544
AsuII TTCGAA 1 cut(s) 670
AvaI CYCGRG 1 cut(s) 429
BaeGI GKGCMC 2 cut(s) 593, 616
BanI GGYRCC 1 cut(s) 689
BanII GRGCYC 2 cut(s) 77, 456
BbsI GAAGAC 1 cut(s) 346
Bbv12I GWGCWC 4 cut(s) 77, 456, 605, 786
BbvI GCAGC 4 cut(s) 213, 286, 341, 739
BccI CCATC 5 cut(s) 110, 161, 186, 588, 647
BcgI CGANNNNNNTGC 2 cut(s) 716, 750
BciT130I CCWGG 2 cut(s) 264, 309
BcoDI GTCTC 4 cut(s) 278, 404, 557, 708
BfaI CTAG 3 cut(s) 575, 609, 708
BfuAI ACCTGC 2 cut(s) 383, 473
BglII AGATCT 1 cut(s) 250
BisI GCNGC 4 cut(s) 227, 300, 330, 728
BlsI GCNGC 4 cut(s) 228, 301, 331, 729
Bme1390I CCNGG 2 cut(s) 264, 309
BmeT110I CYCGRG 1 cut(s) 429
BmiI GGNNCC 2 cut(s) 261, 691
BmrFI CCNGG 2 cut(s) 264, 309
BmsI GCATC 1 cut(s) 117
BpiI GAAGAC 1 cut(s) 346
Bpu14I TTCGAA 1 cut(s) 670
BpuEI CTTGAG 1 cut(s) 302
BsaBI GATNNNNATC 1 cut(s) 131
BsaHI GRCGYC 1 cut(s) 755
BsaJI CCNNGG 2 cut(s) 263, 693
BsaWI WCCGGW 1 cut(s) 237
BsaXI ACNNNNNCTCC 2 cut(s) 443, 473
Bse118I RCCGGY 1 cut(s) 237
Bse1I ACTGG 1 cut(s) 787
Bse8I GATNNNNATC 1 cut(s) 131
BseBI CCWGG 2 cut(s) 264, 309
BseDI CCNNGG 2 cut(s) 263, 693
BseGI GGATG 2 cut(s) 125, 153
BseJI GATNNNNATC 1 cut(s) 131
BseNI ACTGG 1 cut(s) 787
BseRI GAGGAG 4 cut(s) 20, 32, 55, 67
BseSI GKGCMC 2 cut(s) 593, 616
BseXI GCAGC 4 cut(s) 213, 286, 341, 739
BsgI GTGCAG 1 cut(s) 411
BshFI GGCC 1 cut(s) 307
BshNI GGYRCC 1 cut(s) 689
BshTI ACCGGT 1 cut(s) 237
BsiHKAI GWGCWC 4 cut(s) 77, 456, 605, 786
BsiHKCI CYCGRG 1 cut(s) 429
BsiSI CCGG 1 cut(s) 238
BsmAI GTCTC 4 cut(s) 278, 404, 557, 708
BsmBI CGTCTC 2 cut(s) 404, 557
BsnI GGCC 1 cut(s) 307
BsoBI CYCGRG 1 cut(s) 429
Bsp119I TTCGAA 1 cut(s) 670
Bsp1286I GDGCHC 6 cut(s) 77, 456, 593, 605, 616, 786
Bsp143I GATC 3 cut(s) 160, 250, 759
BspACI CCGC 4 cut(s) 174, 336, 525, 711
BspANI GGCC 1 cut(s) 307
BspLI GGNNCC 2 cut(s) 261, 691
BspMI ACCTGC 2 cut(s) 383, 473
BspT104I TTCGAA 1 cut(s) 670
BspT107I GGYRCC 1 cut(s) 689
BsrBI CCGCTC 1 cut(s) 525
BsrFI RCCGGY 1 cut(s) 237
BsrI ACTGG 1 cut(s) 787
BssAI RCCGGY 1 cut(s) 237
BssECI CCNNGG 2 cut(s) 263, 693
BssMI GATC 3 cut(s) 160, 250, 759
BssNI GRCGYC 1 cut(s) 755
BssT1I CCWWGG 1 cut(s) 693
Bst2UI CCWGG 2 cut(s) 264, 309
Bst4CI ACNGT 3 cut(s) 443, 487, 622
BstACI GRCGYC 1 cut(s) 755
BstBI TTCGAA 1 cut(s) 670
BstC8I GCNNGC 2 cut(s) 334, 512
BstF5I GGATG 2 cut(s) 125, 153
BstKTI GATC 3 cut(s) 163, 253, 762
BstMAI GTCTC 4 cut(s) 278, 404, 557, 708
BstMBI GATC 3 cut(s) 160, 250, 759
BstMWI GCNNNNNNNGC 1 cut(s) 296
BstNI CCWGG 2 cut(s) 264, 309
BstNSI RCATGY 1 cut(s) 210
BstSCI CCNGG 2 cut(s) 262, 307
BstSLI GKGCMC 2 cut(s) 593, 616
BstV1I GCAGC 4 cut(s) 213, 286, 341, 739
BstV2I GAAGAC 1 cut(s) 346
BstX2I RGATCY 1 cut(s) 250
BstYI RGATCY 1 cut(s) 250
BsuRI GGCC 1 cut(s) 307
BtsCI GGATG 2 cut(s) 125, 153
BtsIMutI CAGTG 1 cut(s) 448
BveI ACCTGC 2 cut(s) 383, 473
Cac8I GCNNGC 2 cut(s) 334, 512
Cfr10I RCCGGY 1 cut(s) 237
CspAI ACCGGT 1 cut(s) 237
CspCI CAANNNNNGTGG 2 cut(s) 17, 52
CviAII CATG 2 cut(s) 93, 207
DpnI GATC 3 cut(s) 162, 252, 761
DpnII GATC 3 cut(s) 160, 250, 759
DrdI GACNNNNNNGTC 1 cut(s) 242
DseDI GACNNNNNNGTC 1 cut(s) 242
EciI GGCGGA 1 cut(s) 351
Ecl136II GAGCTC 2 cut(s) 75, 454
Eco130I CCWWGG 1 cut(s) 693
Eco24I GRGCYC 2 cut(s) 77, 456
Eco53kI GAGCTC 2 cut(s) 75, 454
Eco57I CTGAAG 1 cut(s) 121
Eco88I CYCGRG 1 cut(s) 429
EcoICRI GAGCTC 2 cut(s) 75, 454
EcoRII CCWGG 2 cut(s) 262, 307
EcoT14I CCWWGG 1 cut(s) 693
EcoT38I GRGCYC 2 cut(s) 77, 456
ErhI CCWWGG 1 cut(s) 693
Esp3I CGTCTC 2 cut(s) 404, 557
FaeI CATG 2 cut(s) 96, 210
FaiI YATR 9 cut(s) 94, 101, 168, 208, 354, 419, 421, 519, 747
FalI AAGNNNNNCTT 2 cut(s) 686, 718
FatI CATG 2 cut(s) 92, 206
FblI GTMKAC 1 cut(s) 461
Fnu4HI GCNGC 4 cut(s) 227, 300, 330, 728
FokI GGATG 2 cut(s) 132, 140
FriOI GRGCYC 2 cut(s) 77, 456
Fsp4HI GCNGC 4 cut(s) 227, 300, 330, 728
FspBI CTAG 3 cut(s) 575, 609, 708
GluI GCNGC 4 cut(s) 227, 300, 330, 728
HaeIII GGCC 1 cut(s) 307
HapII CCGG 1 cut(s) 238
Hin1I GRCGYC 1 cut(s) 755
Hin1II CATG 2 cut(s) 96, 210
HincII GTYRAC 2 cut(s) 235, 462
HindII GTYRAC 2 cut(s) 235, 462
HinfI GANTC 1 cut(s) 568
HpaII CCGG 1 cut(s) 238
HphI GGTGA 3 cut(s) 203, 407, 544
Hpy166II GTNNAC 2 cut(s) 235, 462
Hpy188I TCNGA 3 cut(s) 126, 439, 753
Hpy188III TCNNGA 4 cut(s) 110, 429, 575, 703
Hpy8I GTNNAC 2 cut(s) 235, 462
Hpy99I CGWCG 1 cut(s) 760
HpyAV CCTTC 3 cut(s) 484, 522, 677
HpyCH4III ACNGT 3 cut(s) 443, 487, 622
HpyCH4IV ACGT 2 cut(s) 755, 777
HpyCH4V TGCA 7 cut(s) 130, 302, 329, 392, 446, 468, 727
HpyF10VI GCNNNNNNNGC 1 cut(s) 296
HpySE526I ACGT 2 cut(s) 755, 777
Hsp92I GRCGYC 1 cut(s) 755
Hsp92II CATG 2 cut(s) 96, 210
Kzo9I GATC 3 cut(s) 160, 250, 759
LmnI GCTCC 6 cut(s) 72, 80, 259, 451, 459, 522
Lsp1109I GCAGC 4 cut(s) 213, 286, 341, 739
LweI GCATC 1 cut(s) 117
MaeI CTAG 3 cut(s) 575, 609, 708
MaeII ACGT 2 cut(s) 755, 777
MaeIII GTNAC 2 cut(s) 209, 532
MalI GATC 3 cut(s) 162, 252, 761
MbiI CCGCTC 1 cut(s) 525
MboI GATC 3 cut(s) 160, 250, 759
MboII GAAGA 5 cut(s) 127, 351, 621, 754, 757
MflI RGATCY 1 cut(s) 250
MhlI GDGCHC 6 cut(s) 77, 456, 593, 605, 616, 786
MluCI AATT 3 cut(s) 324, 470, 678
MspA1I CMGCKG 1 cut(s) 290
MspI CCGG 1 cut(s) 238
MspR9I CCNGG 2 cut(s) 264, 309
MvaI CCWGG 2 cut(s) 264, 309
MwoI GCNNNNNNNGC 1 cut(s) 296
NdeII GATC 3 cut(s) 160, 250, 759
NlaIII CATG 2 cut(s) 96, 210
NlaIV GGNNCC 2 cut(s) 261, 691
NmuCI GTSAC 2 cut(s) 209, 532
NspI RCATGY 1 cut(s) 210
NspV TTCGAA 1 cut(s) 670
PaeR7I CTCGAG 1 cut(s) 429
PaqCI CACCTGC 1 cut(s) 383
PfeI GAWTC 1 cut(s) 568
PinAI ACCGGT 1 cut(s) 237
PkrI GCNGC 4 cut(s) 228, 301, 331, 729
PsiI TTATAA 2 cut(s) 101, 354
Psp124BI GAGCTC 2 cut(s) 77, 456
Psp6I CCWGG 2 cut(s) 262, 307
PspGI CCWGG 2 cut(s) 262, 307
PspN4I GGNNCC 2 cut(s) 261, 691
PsuI RGATCY 1 cut(s) 250
PvuII CAGCTG 1 cut(s) 290
SacI GAGCTC 2 cut(s) 77, 456
SalI GTCGAC 1 cut(s) 460
SatI GCNGC 4 cut(s) 227, 300, 330, 728
Sau3AI GATC 3 cut(s) 160, 250, 759
ScrFI CCNGG 2 cut(s) 264, 309
SduI GDGCHC 6 cut(s) 77, 456, 593, 605, 616, 786
SfaNI GCATC 1 cut(s) 117
Sfr274I CTCGAG 1 cut(s) 429
SfuI TTCGAA 1 cut(s) 670
SlaI CTCGAG 1 cut(s) 429
SmlI CTYRAG 2 cut(s) 281, 429
SmoI CTYRAG 2 cut(s) 281, 429
Sse9I AATT 3 cut(s) 324, 470, 678
SsiI CCGC 4 cut(s) 174, 336, 525, 711
SspI AATATT 1 cut(s) 358
SspMI CTAG 3 cut(s) 575, 609, 708
SstI GAGCTC 2 cut(s) 77, 456
StyD4I CCNGG 2 cut(s) 262, 307
StyI CCWWGG 1 cut(s) 693
TaaI ACNGT 3 cut(s) 443, 487, 622
TaiI ACGT 2 cut(s) 758, 780
TaqI TCGA 6 cut(s) 430, 461, 670, 676, 736, 758
TasI AATT 3 cut(s) 324, 470, 678
TfiI GAWTC 1 cut(s) 568
TscAI CASTG 1 cut(s) 448
TseFI GTSAC 2 cut(s) 209, 532
TseI GCWGC 4 cut(s) 226, 299, 329, 727
Tsp45I GTSAC 2 cut(s) 209, 532
TspDTI ATGAA 1 cut(s) 506
TspRI CASTG 1 cut(s) 448
XbaI TCTAGA 1 cut(s) 574
XceI RCATGY 1 cut(s) 210
XhoI CTCGAG 1 cut(s) 429
XmiI GTMKAC 1 cut(s) 461
XspI CTAG 3 cut(s) 575, 609, 708
ZraI GACGTC 1 cut(s) 756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.