pycom02g24570

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0007291
Physical Location & Seq
Reverse (-)
22697222 .. 22700255
3034 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g24570.2

Sequence Viewer

Length: 858 bp
ATGAACCTTAACATTCCAAAGAGATCTCTCTTGCAAGAGTCTAGAGACGCAGAGAGGGAAGAGGCTATGGAGGACGTGGTGGAGGAGATAGAGCAAAAAAGGTGGAAGAGAAGCCTCACAAAGACAAAGAGAAGCAGGAGCTCCTCAAAGTCGTCCTCATGCCTTATAAATAATCTTGATGATGGATGTCTGATGCACATCTTCAGCTTCCTCTCTCCCATTCCAGATCGGTATAACACCGCCCTCGTTTGCCACAGATGGCTTTACTTGGCATGTCACCCTCGCTTGTGGCTGCGAGTTGACCGGTCCGCCAAAGATCTTTTGGAGCCTGGGGTTTTCCCCAACCTTGAGACAGCTGTTGAGGCTGCAAGGCCAGGCGATACTATTCTAATTTCGGCAGGCGGAAGACATCTTGCTTATAATATTCAAATAAAAAAGCGACTCTGCCTGATTGGTGCAGGTGAGCAACCTGATGAGACGACTATATATTGTTCTCGAGGTTCTGACAGTGCATTGGAGCTCCTGTCGACCTGCAAATTGGCGAACTTGACTGTGAAGGCAGAGCTTGGCTGTTGCTTGCTTCATAGGAGCGGAAGGGTGACGATAGAGGGGTGTGTGCTTCAATGCGAGACGAATCCTCTAGACCATCTTTCGTGCCCTATTGTGAGCACAGCTAGTGCCCAGACGGTAATATCTTCCCCTGTGAAATGTTCTAATGATGGCGTTTCTGTTTTTCGAACTCGAATTGAAGGAGGTGCCAAGGCTGTCTTGACTAGCGGAGACTTGACCTTGCAGCGAGTTCGAGTTATTTATGCTCGGACGTCAATCTTTTTCTGGTTTGACGTAGAGCACCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

286

Amino Acids

31.78

Weight (kDa)

8.21

Isoelectric Point (pI)

70.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 167, 420
AarI CACCTGC 1 cut(s) 449
AatII GACGTC 1 cut(s) 824
Acc36I ACCTGC 2 cut(s) 449, 539
AccB1I GGYRCC 1 cut(s) 755
AccBSI CCGCTC 1 cut(s) 591
AccI GTMKAC 1 cut(s) 527
AciI CCGC 5 cut(s) 240, 309, 402, 591, 777
AcuI CTGAAG 1 cut(s) 187
AcyI GRCGYC 1 cut(s) 821
AgeI ACCGGT 1 cut(s) 303
AgsI TTSAA 3 cut(s) 428, 623, 749
AjiI CACGTC 1 cut(s) 76
AjnI CCWGG 2 cut(s) 328, 373
AluBI AGCT 6 cut(s) 141, 207, 356, 520, 565, 674
AluI AGCT 6 cut(s) 141, 207, 356, 520, 565, 674
Alw21I GWGCWC 4 cut(s) 143, 522, 671, 852
Alw26I GTCTC 5 cut(s) 39, 344, 470, 623, 774
Ama87I CYCGRG 1 cut(s) 495
AoxI GGCC 1 cut(s) 371
ApeKI GCWGC 3 cut(s) 292, 365, 793
AsiGI ACCGGT 1 cut(s) 303
AspS9I GGNCC 1 cut(s) 306
AsuHPI GGTGA 3 cut(s) 269, 473, 610
AsuII TTCGAA 1 cut(s) 736
AvaI CYCGRG 1 cut(s) 495
AvaII GGWCC 1 cut(s) 306
BaeGI GKGCMC 2 cut(s) 659, 682
BanI GGYRCC 1 cut(s) 755
BanII GRGCYC 2 cut(s) 143, 522
BbsI GAAGAC 1 cut(s) 412
Bbv12I GWGCWC 4 cut(s) 143, 522, 671, 852
BbvI GCAGC 3 cut(s) 279, 352, 805
BccI CCATC 4 cut(s) 176, 252, 654, 713
BcgI CGANNNNNNTGC 2 cut(s) 782, 816
BciT130I CCWGG 2 cut(s) 330, 375
BcoDI GTCTC 5 cut(s) 39, 344, 470, 623, 774
BfaI CTAG 4 cut(s) 42, 641, 675, 774
BfuAI ACCTGC 2 cut(s) 449, 539
BglII AGATCT 2 cut(s) 23, 316
BisI GCNGC 3 cut(s) 293, 366, 794
BlsI GCNGC 3 cut(s) 294, 367, 795
Bme1390I CCNGG 2 cut(s) 330, 375
Bme18I GGWCC 1 cut(s) 306
BmeT110I CYCGRG 1 cut(s) 495
BmgBI CACGTC 1 cut(s) 76
BmgT120I GGNCC 1 cut(s) 306
BmiI GGNNCC 2 cut(s) 327, 757
BmrFI CCNGG 2 cut(s) 330, 375
BmsI GCATC 1 cut(s) 183
BpiI GAAGAC 1 cut(s) 412
Bpu14I TTCGAA 1 cut(s) 736
BpuEI CTTGAG 1 cut(s) 368
BsaBI GATNNNNATC 1 cut(s) 197
BsaHI GRCGYC 1 cut(s) 821
BsaJI CCNNGG 2 cut(s) 329, 759
BsaWI WCCGGW 1 cut(s) 303
BsaXI ACNNNNNCTCC 2 cut(s) 509, 539
Bse118I RCCGGY 1 cut(s) 303
Bse1I ACTGG 1 cut(s) 853
Bse8I GATNNNNATC 1 cut(s) 197
BseBI CCWGG 2 cut(s) 330, 375
BseDI CCNNGG 2 cut(s) 329, 759
BseGI GGATG 1 cut(s) 191
BseJI GATNNNNATC 1 cut(s) 197
BseNI ACTGG 1 cut(s) 853
BseRI GAGGAG 2 cut(s) 98, 133
BseSI GKGCMC 2 cut(s) 659, 682
BseXI GCAGC 3 cut(s) 279, 352, 805
BsgI GTGCAG 1 cut(s) 477
BshFI GGCC 1 cut(s) 373
BshNI GGYRCC 1 cut(s) 755
BshTI ACCGGT 1 cut(s) 303
BsiHKAI GWGCWC 4 cut(s) 143, 522, 671, 852
BsiHKCI CYCGRG 1 cut(s) 495
BsiSI CCGG 1 cut(s) 304
BsmAI GTCTC 5 cut(s) 39, 344, 470, 623, 774
BsmBI CGTCTC 3 cut(s) 39, 470, 623
BsnI GGCC 1 cut(s) 373
BsoBI CYCGRG 1 cut(s) 495
Bsp119I TTCGAA 1 cut(s) 736
Bsp1286I GDGCHC 6 cut(s) 143, 522, 659, 671, 682, 852
Bsp143I GATC 3 cut(s) 23, 226, 316
BspACI CCGC 5 cut(s) 240, 309, 402, 591, 777
BspANI GGCC 1 cut(s) 373
BspLI GGNNCC 2 cut(s) 327, 757
BspMI ACCTGC 2 cut(s) 449, 539
BspT104I TTCGAA 1 cut(s) 736
BspT107I GGYRCC 1 cut(s) 755
BsrBI CCGCTC 1 cut(s) 591
BsrFI RCCGGY 1 cut(s) 303
BsrI ACTGG 1 cut(s) 853
BssAI RCCGGY 1 cut(s) 303
BssECI CCNNGG 2 cut(s) 329, 759
BssMI GATC 3 cut(s) 23, 226, 316
BssNI GRCGYC 1 cut(s) 821
BssT1I CCWWGG 1 cut(s) 759
Bst2UI CCWGG 2 cut(s) 330, 375
Bst4CI ACNGT 3 cut(s) 509, 553, 688
Bst6I CTCTTC 2 cut(s) 54, 101
BstACI GRCGYC 1 cut(s) 821
BstBI TTCGAA 1 cut(s) 736
BstC8I GCNNGC 2 cut(s) 400, 578
BstF5I GGATG 1 cut(s) 191
BstKTI GATC 3 cut(s) 26, 229, 319
BstMAI GTCTC 5 cut(s) 39, 344, 470, 623, 774
BstMBI GATC 3 cut(s) 23, 226, 316
BstMWI GCNNNNNNNGC 1 cut(s) 362
BstNI CCWGG 2 cut(s) 330, 375
BstNSI RCATGY 1 cut(s) 276
BstSCI CCNGG 2 cut(s) 328, 373
BstSLI GKGCMC 2 cut(s) 659, 682
BstV1I GCAGC 3 cut(s) 279, 352, 805
BstV2I GAAGAC 1 cut(s) 412
BstX2I RGATCY 2 cut(s) 23, 316
BstYI RGATCY 2 cut(s) 23, 316
BsuRI GGCC 1 cut(s) 373
BtrI CACGTC 1 cut(s) 76
BtsCI GGATG 1 cut(s) 191
BtsIMutI CAGTG 1 cut(s) 514
BveI ACCTGC 2 cut(s) 449, 539
Cac8I GCNNGC 2 cut(s) 400, 578
Cfr10I RCCGGY 1 cut(s) 303
Cfr13I GGNCC 1 cut(s) 306
CpoI CGGWCCG 1 cut(s) 306
CseI GACGC 1 cut(s) 56
CspAI ACCGGT 1 cut(s) 303
CspCI CAANNNNNGTGG 2 cut(s) 83, 118
CspI CGGWCCG 1 cut(s) 306
CviAII CATG 2 cut(s) 159, 273
DpnI GATC 3 cut(s) 25, 228, 318
DpnII GATC 3 cut(s) 23, 226, 316
Eam1104I CTCTTC 2 cut(s) 54, 101
EarI CTCTTC 2 cut(s) 54, 101
EciI GGCGGA 2 cut(s) 298, 417
Ecl136II GAGCTC 2 cut(s) 141, 520
Eco130I CCWWGG 1 cut(s) 759
Eco24I GRGCYC 2 cut(s) 143, 522
Eco47I GGWCC 1 cut(s) 306
Eco53kI GAGCTC 2 cut(s) 141, 520
Eco57I CTGAAG 1 cut(s) 187
Eco88I CYCGRG 1 cut(s) 495
EcoICRI GAGCTC 2 cut(s) 141, 520
EcoRII CCWGG 2 cut(s) 328, 373
EcoT14I CCWWGG 1 cut(s) 759
EcoT38I GRGCYC 2 cut(s) 143, 522
ErhI CCWWGG 1 cut(s) 759
Esp3I CGTCTC 3 cut(s) 39, 470, 623
FaeI CATG 2 cut(s) 162, 276
FalI AAGNNNNNCTT 2 cut(s) 752, 784
FatI CATG 2 cut(s) 158, 272
FblI GTMKAC 1 cut(s) 527
Fnu4HI GCNGC 3 cut(s) 293, 366, 794
FokI GGATG 1 cut(s) 198
FriOI GRGCYC 2 cut(s) 143, 522
Fsp4HI GCNGC 3 cut(s) 293, 366, 794
FspBI CTAG 4 cut(s) 42, 641, 675, 774
GluI GCNGC 3 cut(s) 293, 366, 794
HaeIII GGCC 1 cut(s) 373
HapII CCGG 1 cut(s) 304
HgaI GACGC 1 cut(s) 56
Hin1I GRCGYC 1 cut(s) 821
Hin1II CATG 2 cut(s) 162, 276
HincII GTYRAC 2 cut(s) 301, 528
HindII GTYRAC 2 cut(s) 301, 528
HinfI GANTC 3 cut(s) 38, 441, 634
HpaII CCGG 1 cut(s) 304
HphI GGTGA 3 cut(s) 269, 473, 610
Hpy166II GTNNAC 2 cut(s) 301, 528
Hpy188I TCNGA 3 cut(s) 192, 505, 819
Hpy188III TCNNGA 6 cut(s) 42, 176, 224, 495, 641, 769
Hpy8I GTNNAC 2 cut(s) 301, 528
HpyAV CCTTC 3 cut(s) 550, 588, 743
HpyCH4III ACNGT 3 cut(s) 509, 553, 688
HpyCH4IV ACGT 3 cut(s) 75, 821, 843
HpyCH4V TGCA 7 cut(s) 34, 196, 368, 458, 512, 534, 793
HpyF10VI GCNNNNNNNGC 1 cut(s) 362
HpySE526I ACGT 3 cut(s) 75, 821, 843
Hsp92I GRCGYC 1 cut(s) 821
Hsp92II CATG 2 cut(s) 162, 276
Kzo9I GATC 3 cut(s) 23, 226, 316
LmnI GCTCC 6 cut(s) 138, 146, 325, 517, 525, 588
Lsp1109I GCAGC 3 cut(s) 279, 352, 805
LweI GCATC 1 cut(s) 183
MaeI CTAG 4 cut(s) 42, 641, 675, 774
MaeII ACGT 3 cut(s) 75, 821, 843
MaeIII GTNAC 2 cut(s) 275, 598
MalI GATC 3 cut(s) 25, 228, 318
MbiI CCGCTC 1 cut(s) 591
MboI GATC 3 cut(s) 23, 226, 316
MboII GAAGA 5 cut(s) 71, 118, 193, 417, 687
MflI RGATCY 2 cut(s) 23, 316
MhlI GDGCHC 6 cut(s) 143, 522, 659, 671, 682, 852
MluCI AATT 3 cut(s) 390, 536, 744
MlyI GAGTC 2 cut(s) 47, 435
MseI TTAA 1 cut(s) 9
MspA1I CMGCKG 1 cut(s) 356
MspI CCGG 1 cut(s) 304
MspR9I CCNGG 2 cut(s) 330, 375
MvaI CCWGG 2 cut(s) 330, 375
MwoI GCNNNNNNNGC 1 cut(s) 362
NdeII GATC 3 cut(s) 23, 226, 316
NlaIII CATG 2 cut(s) 162, 276
NlaIV GGNNCC 2 cut(s) 327, 757
NmuCI GTSAC 2 cut(s) 275, 598
NspI RCATGY 1 cut(s) 276
NspV TTCGAA 1 cut(s) 736
PaeR7I CTCGAG 1 cut(s) 495
PaqCI CACCTGC 1 cut(s) 449
PfeI GAWTC 1 cut(s) 634
PinAI ACCGGT 1 cut(s) 303
PkrI GCNGC 3 cut(s) 294, 367, 795
PleI GAGTC 2 cut(s) 46, 435
PpsI GAGTC 2 cut(s) 46, 435
PsiI TTATAA 2 cut(s) 167, 420
Psp124BI GAGCTC 2 cut(s) 143, 522
Psp6I CCWGG 2 cut(s) 328, 373
PspGI CCWGG 2 cut(s) 328, 373
PspN4I GGNNCC 2 cut(s) 327, 757
PspPI GGNCC 1 cut(s) 306
PsuI RGATCY 2 cut(s) 23, 316
PvuII CAGCTG 1 cut(s) 356
Rsr2I CGGWCCG 1 cut(s) 306
RsrII CGGWCCG 1 cut(s) 306
SacI GAGCTC 2 cut(s) 143, 522
SalI GTCGAC 1 cut(s) 526
SaqAI TTAA 1 cut(s) 9
SatI GCNGC 3 cut(s) 293, 366, 794
Sau3AI GATC 3 cut(s) 23, 226, 316
Sau96I GGNCC 1 cut(s) 306
SchI GAGTC 2 cut(s) 47, 435
ScrFI CCNGG 2 cut(s) 330, 375
SduI GDGCHC 6 cut(s) 143, 522, 659, 671, 682, 852
SfaNI GCATC 1 cut(s) 183
Sfr274I CTCGAG 1 cut(s) 495
SfuI TTCGAA 1 cut(s) 736
SinI GGWCC 1 cut(s) 306
SlaI CTCGAG 1 cut(s) 495
SmlI CTYRAG 2 cut(s) 347, 495
SmoI CTYRAG 2 cut(s) 347, 495
Sse9I AATT 3 cut(s) 390, 536, 744
SsiI CCGC 5 cut(s) 240, 309, 402, 591, 777
SspI AATATT 1 cut(s) 424
SspMI CTAG 4 cut(s) 42, 641, 675, 774
SstI GAGCTC 2 cut(s) 143, 522
StyD4I CCNGG 2 cut(s) 328, 373
StyI CCWWGG 1 cut(s) 759
TaaI ACNGT 3 cut(s) 509, 553, 688
TaiI ACGT 3 cut(s) 78, 824, 846
TaqI TCGA 5 cut(s) 496, 527, 736, 742, 802
TasI AATT 3 cut(s) 390, 536, 744
TfiI GAWTC 1 cut(s) 634
Tru1I TTAA 1 cut(s) 9
Tru9I TTAA 1 cut(s) 9
TscAI CASTG 1 cut(s) 514
TseFI GTSAC 2 cut(s) 275, 598
TseI GCWGC 3 cut(s) 292, 365, 793
Tsp45I GTSAC 2 cut(s) 275, 598
TspDTI ATGAA 2 cut(s) 17, 572
TspRI CASTG 1 cut(s) 514
VpaK11BI GGWCC 1 cut(s) 306
XbaI TCTAGA 2 cut(s) 41, 640
XceI RCATGY 1 cut(s) 276
XcmI CCANNNNNNNNNTGG 1 cut(s) 319
XhoI CTCGAG 1 cut(s) 495
XmiI GTMKAC 1 cut(s) 527
XspI CTAG 4 cut(s) 42, 641, 675, 774
ZraI GACGTC 1 cut(s) 822
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.