Prupe.2G032200_v2.0.a1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0007291
Physical Location & Seq
Forward (+)
3480179 .. 3484045
3867 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G032200.1

Sequence Viewer

Length: 786 bp
ATGGAGGTGGAGGAAGAGAAAATAAAGCAAACGAAGTGGAAGAGAAGAAGCAGCAGCAGCAGCAGCTCATCAAAGTCATCTTCGTGTCTTATAAATAATCTTGATGATGGGTGTCTGATGCACATCTTTAGCTTCCTCCCTCCCATCCCAGATCGGTATAACACCGCCCTCGTTTGCCACAGATGGCTTTTCTTGGCATGTCACCCTCGCTTGTGGCTACGAGTTGAACGATCAGTCAAAGATCGATTGGAGGAGCCTGGAGTTTTCCCCGACATTGAGACTGCTGTTGCTGCTGCAAGGCCTGGTGACACCATTCTAATTGCAGCAGGAGGAAGGCATCTTGCTTCTAATATTCAAATAAAAAAGCGACTTTGCCTGATTGGTGGAGGTGAGCTACCTGATGAAACAACACTCTTTTGTTCTCGAGCTTCAGACAGTGCATTGGAGTTGCTGTCCACCTGCAAATTGGCAAACTTGACTGTGAAGGCAGAACTTGGTTGTTGCTTGCTTCATAGAAATGGAAGGCTGACGATAGAGGGGTGTGTGCTTCAATGCGAATCAAATCCCCTGGACCATCTTTCGTGCCCTATTGTGAGCACTGCTAGTGCCCAGACAGTATTCCCTTCCTCCGTGAAATGTTCTAAGGACGGTGTTTCCGTATTTCGAACTCGAATTGAAGGAGGCGCCAAGGCTGTTCTGACTAGTGGAGACTTGACATTGCAGCGAGTTCGAGTAATTTATGCCCGGACATCGATCTTTTTCTGGTTTGATGTAGAGCACCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

262

Amino Acids

28.82

Weight (kDa)

8.41

Isoelectric Point (pI)

64.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 92
AarI CACCTGC 1 cut(s) 467
Acc36I ACCTGC 1 cut(s) 467
AccB1I GGYRCC 1 cut(s) 683
AciI CCGC 1 cut(s) 165
AcuI CTGAAG 1 cut(s) 414
AcyI GRCGYC 1 cut(s) 684
AgsI TTSAA 4 cut(s) 227, 356, 551, 677
AhlI ACTAGT 1 cut(s) 701
AjnI CCWGG 3 cut(s) 256, 301, 567
AluBI AGCT 4 cut(s) 66, 132, 394, 428
AluI AGCT 4 cut(s) 66, 132, 394, 428
Alw21I GWGCWC 2 cut(s) 599, 780
Alw26I GTCTC 2 cut(s) 272, 702
Ama87I CYCGRG 1 cut(s) 423
AoxI GGCC 1 cut(s) 299
ApeKI GCWGC 9 cut(s) 51, 54, 57, 60, 63, 290, 293, 323, 721
AspLEI GCGC 1 cut(s) 686
AspS9I GGNCC 1 cut(s) 571
AsuC2I CCSGG 1 cut(s) 745
AsuHPI GGTGA 3 cut(s) 194, 317, 401
AsuII TTCGAA 1 cut(s) 664
AvaI CYCGRG 1 cut(s) 423
AvaII GGWCC 1 cut(s) 571
BaeGI GKGCMC 2 cut(s) 587, 610
BanI GGYRCC 1 cut(s) 683
Bbv12I GWGCWC 2 cut(s) 599, 780
BbvI GCAGC 9 cut(s) 63, 66, 69, 72, 75, 277, 280, 335, 733
BccI CCATC 4 cut(s) 101, 152, 177, 582
BcgI CGANNNNNNTGC 2 cut(s) 710, 744
BciT130I CCWGG 3 cut(s) 258, 303, 569
BcnI CCSGG 1 cut(s) 745
BcoDI GTCTC 2 cut(s) 272, 702
BcuI ACTAGT 1 cut(s) 701
BfaI CTAG 2 cut(s) 603, 702
BfoI RGCGCY 1 cut(s) 687
BfuAI ACCTGC 1 cut(s) 467
BisI GCNGC 9 cut(s) 52, 55, 58, 61, 64, 291, 294, 324, 722
BlsI GCNGC 9 cut(s) 53, 56, 59, 62, 65, 292, 295, 325, 723
Bme1390I CCNGG 4 cut(s) 258, 303, 569, 745
Bme18I GGWCC 1 cut(s) 571
BmeT110I CYCGRG 1 cut(s) 423
BmgT120I GGNCC 1 cut(s) 571
BmiI GGNNCC 2 cut(s) 255, 685
BmrFI CCNGG 4 cut(s) 258, 303, 569, 745
BmsI GCATC 2 cut(s) 108, 346
BpmI CTGGAG 1 cut(s) 279
Bpu14I TTCGAA 1 cut(s) 664
BpuMI CCSGG 1 cut(s) 745
Bsa29I ATCGAT 2 cut(s) 244, 752
BsaBI GATNNNNATC 1 cut(s) 122
BsaHI GRCGYC 1 cut(s) 684
BsaJI CCNNGG 2 cut(s) 567, 687
BsaXI ACNNNNNCTCC 2 cut(s) 437, 467
Bse1I ACTGG 1 cut(s) 781
Bse3DI GCAATG 1 cut(s) 716
Bse8I GATNNNNATC 1 cut(s) 122
BseBI CCWGG 3 cut(s) 258, 303, 569
BseCI ATCGAT 2 cut(s) 244, 752
BseDI CCNNGG 2 cut(s) 567, 687
BseGI GGATG 1 cut(s) 144
BseJI GATNNNNATC 1 cut(s) 122
BseMI GCAATG 1 cut(s) 716
BseNI ACTGG 1 cut(s) 781
BseRI GAGGAG 1 cut(s) 266
BseSI GKGCMC 2 cut(s) 587, 610
BseXI GCAGC 9 cut(s) 63, 66, 69, 72, 75, 277, 280, 335, 733
BshFI GGCC 1 cut(s) 301
BshNI GGYRCC 1 cut(s) 683
BshVI ATCGAT 2 cut(s) 244, 752
BsiHKAI GWGCWC 2 cut(s) 599, 780
BsiHKCI CYCGRG 1 cut(s) 423
BsiSI CCGG 1 cut(s) 745
BsmAI GTCTC 2 cut(s) 272, 702
BsnI GGCC 1 cut(s) 301
BsoBI CYCGRG 1 cut(s) 423
Bsp119I TTCGAA 1 cut(s) 664
Bsp1286I GDGCHC 4 cut(s) 587, 599, 610, 780
Bsp143I GATC 4 cut(s) 151, 230, 241, 753
BspACI CCGC 1 cut(s) 165
BspANI GGCC 1 cut(s) 301
BspDI ATCGAT 2 cut(s) 244, 752
BspLI GGNNCC 2 cut(s) 255, 685
BspMI ACCTGC 1 cut(s) 467
BspT104I TTCGAA 1 cut(s) 664
BspT107I GGYRCC 1 cut(s) 683
BsrDI GCAATG 1 cut(s) 716
BsrI ACTGG 1 cut(s) 781
BssECI CCNNGG 2 cut(s) 567, 687
BssMI GATC 4 cut(s) 151, 230, 241, 753
BssNI GRCGYC 1 cut(s) 684
BssT1I CCWWGG 1 cut(s) 687
Bst2UI CCWGG 3 cut(s) 258, 303, 569
Bst4CI ACNGT 4 cut(s) 437, 481, 616, 650
Bst6I CTCTTC 2 cut(s) 9, 35
BstACI GRCGYC 1 cut(s) 684
BstBI TTCGAA 1 cut(s) 664
BstC8I GCNNGC 1 cut(s) 506
BstDEI CTNAG 1 cut(s) 642
BstF5I GGATG 1 cut(s) 144
BstH2I RGCGCY 1 cut(s) 687
BstHHI GCGC 1 cut(s) 686
BstKTI GATC 4 cut(s) 154, 233, 244, 756
BstMAI GTCTC 2 cut(s) 272, 702
BstMBI GATC 4 cut(s) 151, 230, 241, 753
BstMWI GCNNNNNNNGC 4 cut(s) 57, 60, 63, 290
BstNI CCWGG 3 cut(s) 258, 303, 569
BstNSI RCATGY 1 cut(s) 201
BstSCI CCNGG 4 cut(s) 256, 301, 567, 743
BstSLI GKGCMC 2 cut(s) 587, 610
BstV1I GCAGC 9 cut(s) 63, 66, 69, 72, 75, 277, 280, 335, 733
Bsu15I ATCGAT 2 cut(s) 244, 752
BsuRI GGCC 1 cut(s) 301
BsuTUI ATCGAT 2 cut(s) 244, 752
BtsCI GGATG 1 cut(s) 144
BtsI GCAGTG 1 cut(s) 597
BtsIMutI CAGTG 2 cut(s) 442, 597
BveI ACCTGC 1 cut(s) 467
Cac8I GCNNGC 1 cut(s) 506
CfoI GCGC 1 cut(s) 686
Cfr13I GGNCC 1 cut(s) 571
ClaI ATCGAT 2 cut(s) 244, 752
CspCI CAANNNNNGTGG 2 cut(s) 17, 52
CviAII CATG 1 cut(s) 198
DdeI CTNAG 1 cut(s) 642
DinI GGCGCC 1 cut(s) 685
DpnI GATC 4 cut(s) 153, 232, 243, 755
DpnII GATC 4 cut(s) 151, 230, 241, 753
Eam1104I CTCTTC 2 cut(s) 9, 35
EarI CTCTTC 2 cut(s) 9, 35
Eco130I CCWWGG 1 cut(s) 687
Eco147I AGGCCT 1 cut(s) 301
Eco47I GGWCC 1 cut(s) 571
Eco57I CTGAAG 1 cut(s) 414
Eco88I CYCGRG 1 cut(s) 423
EcoRII CCWGG 3 cut(s) 256, 301, 567
EcoT14I CCWWGG 1 cut(s) 687
EgeI GGCGCC 1 cut(s) 685
EheI GGCGCC 1 cut(s) 685
ErhI CCWWGG 1 cut(s) 687
FaeI CATG 1 cut(s) 201
FaiI YATR 5 cut(s) 92, 159, 199, 513, 741
FatI CATG 1 cut(s) 197
Fnu4HI GCNGC 9 cut(s) 52, 55, 58, 61, 64, 291, 294, 324, 722
FokI GGATG 1 cut(s) 131
Fsp4HI GCNGC 9 cut(s) 52, 55, 58, 61, 64, 291, 294, 324, 722
FspBI CTAG 2 cut(s) 603, 702
GlaI GCGC 1 cut(s) 685
GluI GCNGC 9 cut(s) 52, 55, 58, 61, 64, 291, 294, 324, 722
GsuI CTGGAG 1 cut(s) 279
HaeII RGCGCY 1 cut(s) 687
HaeIII GGCC 1 cut(s) 301
HapII CCGG 1 cut(s) 745
HhaI GCGC 1 cut(s) 686
Hin1I GRCGYC 1 cut(s) 684
Hin1II CATG 1 cut(s) 201
Hin6I GCGC 1 cut(s) 684
HinP1I GCGC 1 cut(s) 684
HinfI GANTC 1 cut(s) 557
HpaII CCGG 1 cut(s) 745
HphI GGTGA 3 cut(s) 194, 317, 401
Hpy166II GTNNAC 1 cut(s) 456
Hpy188I TCNGA 3 cut(s) 117, 433, 699
Hpy188III TCNNGA 2 cut(s) 101, 423
Hpy8I GTNNAC 1 cut(s) 456
HpyAV CCTTC 5 cut(s) 327, 478, 516, 633, 671
HpyCH4III ACNGT 4 cut(s) 437, 481, 616, 650
HpyCH4V TGCA 6 cut(s) 121, 296, 323, 440, 462, 721
HpyF10VI GCNNNNNNNGC 4 cut(s) 57, 60, 63, 290
HpyF3I CTNAG 1 cut(s) 642
Hsp92I GRCGYC 1 cut(s) 684
Hsp92II CATG 1 cut(s) 201
HspAI GCGC 1 cut(s) 684
KasI GGCGCC 1 cut(s) 683
Kzo9I GATC 4 cut(s) 151, 230, 241, 753
LmnI GCTCC 1 cut(s) 253
Lsp1109I GCAGC 9 cut(s) 63, 66, 69, 72, 75, 277, 280, 335, 733
LweI GCATC 2 cut(s) 108, 346
MaeI CTAG 2 cut(s) 603, 702
MaeIII GTNAC 2 cut(s) 200, 305
MalI GATC 4 cut(s) 153, 232, 243, 755
MboI GATC 4 cut(s) 151, 230, 241, 753
MboII GAAGA 4 cut(s) 26, 52, 57, 72
MhlI GDGCHC 4 cut(s) 587, 599, 610, 780
MluCI AATT 4 cut(s) 318, 464, 672, 735
Mly113I GGCGCC 1 cut(s) 684
MslI CAYNNNNRTG 2 cut(s) 82, 516
MspI CCGG 1 cut(s) 745
MspR9I CCNGG 4 cut(s) 258, 303, 569, 745
MvaI CCWGG 3 cut(s) 258, 303, 569
MwoI GCNNNNNNNGC 4 cut(s) 57, 60, 63, 290
NarI GGCGCC 1 cut(s) 684
NciI CCSGG 1 cut(s) 745
NdeII GATC 4 cut(s) 151, 230, 241, 753
NlaIII CATG 1 cut(s) 201
NlaIV GGNNCC 2 cut(s) 255, 685
NmuCI GTSAC 2 cut(s) 200, 305
NspI RCATGY 1 cut(s) 201
NspV TTCGAA 1 cut(s) 664
PaeR7I CTCGAG 1 cut(s) 423
PaqCI CACCTGC 1 cut(s) 467
PceI AGGCCT 1 cut(s) 301
PcsI WCGNNNNNNNCGW 2 cut(s) 226, 654
PfeI GAWTC 1 cut(s) 557
PkrI GCNGC 9 cut(s) 53, 56, 59, 62, 65, 292, 295, 325, 723
PluTI GGCGCC 1 cut(s) 687
PsiI TTATAA 1 cut(s) 92
Psp6I CCWGG 3 cut(s) 256, 301, 567
PspGI CCWGG 3 cut(s) 256, 301, 567
PspN4I GGNNCC 2 cut(s) 255, 685
PspPI GGNCC 1 cut(s) 571
RseI CAYNNNNRTG 2 cut(s) 82, 516
SatI GCNGC 9 cut(s) 52, 55, 58, 61, 64, 291, 294, 324, 722
Sau3AI GATC 4 cut(s) 151, 230, 241, 753
Sau96I GGNCC 1 cut(s) 571
ScrFI CCNGG 4 cut(s) 258, 303, 569, 745
SduI GDGCHC 4 cut(s) 587, 599, 610, 780
SetI ASST 8 cut(s) 9, 68, 134, 391, 396, 400, 430, 461
SfaNI GCATC 2 cut(s) 108, 346
SfoI GGCGCC 1 cut(s) 685
Sfr274I CTCGAG 1 cut(s) 423
SfuI TTCGAA 1 cut(s) 664
SinI GGWCC 1 cut(s) 571
SlaI CTCGAG 1 cut(s) 423
SmiMI CAYNNNNRTG 2 cut(s) 82, 516
SmlI CTYRAG 1 cut(s) 423
SmoI CTYRAG 1 cut(s) 423
SpeI ACTAGT 1 cut(s) 701
Sse9I AATT 4 cut(s) 318, 464, 672, 735
SseBI AGGCCT 1 cut(s) 301
SsiI CCGC 1 cut(s) 165
SspDI GGCGCC 1 cut(s) 683
SspI AATATT 1 cut(s) 352
SspMI CTAG 2 cut(s) 603, 702
StuI AGGCCT 1 cut(s) 301
StyD4I CCNGG 4 cut(s) 256, 301, 567, 743
StyI CCWWGG 1 cut(s) 687
TaaI ACNGT 4 cut(s) 437, 481, 616, 650
TaqI TCGA 6 cut(s) 244, 424, 664, 670, 730, 752
TasI AATT 4 cut(s) 318, 464, 672, 735
TfiI GAWTC 1 cut(s) 557
TscAI CASTG 2 cut(s) 442, 604
TseFI GTSAC 2 cut(s) 200, 305
TseI GCWGC 9 cut(s) 51, 54, 57, 60, 63, 290, 293, 323, 721
Tsp45I GTSAC 2 cut(s) 200, 305
TspDTI ATGAA 2 cut(s) 417, 500
TspGWI ACGGA 2 cut(s) 619, 646
TspRI CASTG 2 cut(s) 442, 604
VpaK11BI GGWCC 1 cut(s) 571
XceI RCATGY 1 cut(s) 201
XcmI CCANNNNNNNNNTGG 1 cut(s) 463
XhoI CTCGAG 1 cut(s) 423
XspI CTAG 2 cut(s) 603, 702
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.