Rroxscaffold_4G00327350

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0007291
Physical Location & Seq
Reverse (-)
60027979 .. 60031158
3180 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00327350.1

Sequence Viewer

Length: 789 bp
ATGGAGGTGGGAGAAGAGCTGAAGCAACCAAAGTGGAAGAGAAGTGTTCCAAAGAGATTCAGCTCACCAAAGTCATCTTCTTCTTTGTCTTGTCTTATAAACAATCTTGATGATGGCTGCCTCATGCACATCTTTAGCTTCCTCTCTCCTATCCCAGATCGCTATAACACCGCCCTGGTTTGCCACAGATGGCTTTTCTTGGCATGTCACCCTCGCTTGTGGCTGCGAGTTGAACGATCAATTAAAGATCCATTGGAGCCTGGCGTCTTCCCCAGTATTGAGACAGCGGTTGCTGCCGCAAGGCCTGGCGACACCATTCTAATTTCAGCAGGGGGAAGGCATCTTGCTTCTGATATTCAAATAAAAAAGCCGCTTTGCCTGATTGGTGGAGGTGAGGTACCGGATGAGACAACCCTCATTTGTTCTCGCGGTGCAGACAGTGCATTGGAGTTCCTGTCCACCTGCAAATTGGCAAACTTAACTGTGAAGGCAGAGCTGGGTTCTTGCTTGCTTCATAGAAGTGGAAGGCTCACAATAGATGGGTGCGTGCTTCAGTGCGAGACGAATCCCCTAGACTTCCTTTCGGTCCCCATTGTGAGTACAGCAAGTGCGCAAACTGTGTTGTCTTCTTCGGTGAAGTGTTATAAGGATGGTGTTTCTGTTTCTCGGACTCGAATTGAAGGAGGTGCCAAGGCTGTTTCAACTAGTGGATACCTGACATTGCAGCGAGTACGAGTCATTTATGCTCGGACGTCTCTCGTTTTCTGGTTTGATATGGAGCAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

262

Amino Acids

28.67

Weight (kDa)

8.64

Isoelectric Point (pI)

57.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 41 - 77 7.8e-09 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 98, 645
AarI CACCTGC 1 cut(s) 470
AatII GACGTC 1 cut(s) 755
Acc16I TGCGCA 1 cut(s) 612
Acc36I ACCTGC 1 cut(s) 470
Acc65I GGTACC 1 cut(s) 397
AccB1I GGYRCC 2 cut(s) 397, 686
AccII CGCG 1 cut(s) 429
AciI CCGC 5 cut(s) 171, 287, 297, 371, 429
AclWI GGATC 1 cut(s) 242
AcuI CTGAAG 2 cut(s) 41, 536
AcyI GRCGYC 2 cut(s) 264, 752
AfaI GTAC 3 cut(s) 399, 601, 732
AgsI TTSAA 4 cut(s) 233, 359, 680, 702
AhlI ACTAGT 1 cut(s) 704
AjnI CCWGG 3 cut(s) 174, 259, 304
AjuI GAANNNNNNNTTGG 2 cut(s) 61, 93
AluBI AGCT 4 cut(s) 19, 63, 138, 496
AluI AGCT 4 cut(s) 19, 63, 138, 496
Alw26I GTCTC 4 cut(s) 275, 401, 554, 759
AlwI GGATC 1 cut(s) 242
AoxI GGCC 1 cut(s) 302
ApeKI GCWGC 4 cut(s) 117, 223, 293, 724
Asp718I GGTACC 1 cut(s) 397
AspLEI GCGC 1 cut(s) 613
AspS9I GGNCC 1 cut(s) 586
AsuHPI GGTGA 4 cut(s) 57, 200, 404, 646
AvaII GGWCC 1 cut(s) 586
BanI GGYRCC 2 cut(s) 397, 686
BbsI GAAGAC 2 cut(s) 259, 618
BbvI GCAGC 4 cut(s) 104, 210, 280, 736
BccI CCATC 4 cut(s) 107, 183, 533, 644
BciT130I CCWGG 3 cut(s) 176, 261, 306
BciVI GTATCC 1 cut(s) 704
BcoDI GTCTC 4 cut(s) 275, 401, 554, 759
BcuI ACTAGT 1 cut(s) 704
BfaI CTAG 2 cut(s) 572, 705
BfuAI ACCTGC 1 cut(s) 470
BfuI GTATCC 1 cut(s) 704
BisI GCNGC 6 cut(s) 118, 224, 294, 297, 371, 725
BlsI GCNGC 6 cut(s) 119, 225, 295, 298, 372, 726
Bme1390I CCNGG 3 cut(s) 176, 261, 306
Bme18I GGWCC 1 cut(s) 586
BmgT120I GGNCC 1 cut(s) 586
BmiI GGNNCC 4 cut(s) 258, 399, 588, 688
BmrFI CCNGG 3 cut(s) 176, 261, 306
BmrI ACTGGG 1 cut(s) 267
BmsI GCATC 1 cut(s) 349
BmuI ACTGGG 1 cut(s) 267
BpiI GAAGAC 2 cut(s) 259, 618
BsaHI GRCGYC 2 cut(s) 264, 752
BsaJI CCNNGG 2 cut(s) 174, 690
BsaWI WCCGGW 1 cut(s) 400
BsaXI ACNNNNNCTCC 4 cut(s) 381, 411, 440, 470
Bse1I ACTGG 1 cut(s) 273
Bse3DI GCAATG 1 cut(s) 719
BseBI CCWGG 3 cut(s) 176, 261, 306
BseDI CCNNGG 2 cut(s) 174, 690
BseGI GGATG 2 cut(s) 409, 655
BseMI GCAATG 1 cut(s) 719
BseNI ACTGG 1 cut(s) 273
BseXI GCAGC 4 cut(s) 104, 210, 280, 736
BseYI CCCAGC 1 cut(s) 496
BsgI GTGCAG 1 cut(s) 453
Bsh1236I CGCG 1 cut(s) 429
BshFI GGCC 1 cut(s) 304
BshNI GGYRCC 2 cut(s) 397, 686
BsiSI CCGG 1 cut(s) 401
BslFI GGGAC 1 cut(s) 572
BsmAI GTCTC 4 cut(s) 275, 401, 554, 759
BsmBI CGTCTC 2 cut(s) 554, 759
BsmFI GGGAC 1 cut(s) 572
BsnI GGCC 1 cut(s) 304
Bsp143I GATC 3 cut(s) 157, 236, 247
BspACI CCGC 5 cut(s) 171, 287, 297, 371, 429
BspANI GGCC 1 cut(s) 304
BspFNI CGCG 1 cut(s) 429
BspLI GGNNCC 4 cut(s) 258, 399, 588, 688
BspMI ACCTGC 1 cut(s) 470
BspPI GGATC 1 cut(s) 242
BspQI GCTCTTC 1 cut(s) 9
BspT107I GGYRCC 2 cut(s) 397, 686
BsrDI GCAATG 1 cut(s) 719
BsrI ACTGG 1 cut(s) 273
BssECI CCNNGG 2 cut(s) 174, 690
BssMI GATC 3 cut(s) 157, 236, 247
BssNI GRCGYC 2 cut(s) 264, 752
BssT1I CCWWGG 1 cut(s) 690
Bst2UI CCWGG 3 cut(s) 176, 261, 306
Bst4CI ACNGT 3 cut(s) 440, 484, 619
Bst6I CTCTTC 2 cut(s) 9, 32
BstACI GRCGYC 2 cut(s) 264, 752
BstAPI GCANNNNNTGC 1 cut(s) 440
BstC8I GCNNGC 2 cut(s) 509, 548
BstF5I GGATG 2 cut(s) 409, 655
BstFNI CGCG 1 cut(s) 429
BstHHI GCGC 1 cut(s) 613
BstKTI GATC 3 cut(s) 160, 239, 250
BstMAI GTCTC 4 cut(s) 275, 401, 554, 759
BstMBI GATC 3 cut(s) 157, 236, 247
BstMWI GCNNNNNNNGC 2 cut(s) 293, 440
BstNI CCWGG 3 cut(s) 176, 261, 306
BstNSI RCATGY 1 cut(s) 207
BstSCI CCNGG 3 cut(s) 174, 259, 304
BstUI CGCG 1 cut(s) 429
BstV1I GCAGC 4 cut(s) 104, 210, 280, 736
BstV2I GAAGAC 2 cut(s) 259, 618
BstX2I RGATCY 1 cut(s) 247
BstYI RGATCY 1 cut(s) 247
BsuI GTATCC 1 cut(s) 704
BsuRI GGCC 1 cut(s) 304
BtsCI GGATG 2 cut(s) 409, 655
BtsIMutI CAGTG 2 cut(s) 445, 560
BveI ACCTGC 1 cut(s) 470
Cac8I GCNNGC 2 cut(s) 509, 548
CfoI GCGC 1 cut(s) 613
Cfr13I GGNCC 1 cut(s) 586
CseI GACGC 1 cut(s) 253
Csp6I GTAC 3 cut(s) 398, 600, 731
CspCI CAANNNNNGTGG 2 cut(s) 14, 49
CviAII CATG 2 cut(s) 124, 204
CviQI GTAC 3 cut(s) 398, 600, 731
DpnI GATC 3 cut(s) 159, 238, 249
DpnII GATC 3 cut(s) 157, 236, 247
Eam1104I CTCTTC 2 cut(s) 9, 32
EarI CTCTTC 2 cut(s) 9, 32
Eco130I CCWWGG 1 cut(s) 690
Eco147I AGGCCT 1 cut(s) 304
Eco47I GGWCC 1 cut(s) 586
Eco57I CTGAAG 2 cut(s) 41, 536
EcoRII CCWGG 3 cut(s) 174, 259, 304
EcoT14I CCWWGG 1 cut(s) 690
ErhI CCWWGG 1 cut(s) 690
Esp3I CGTCTC 2 cut(s) 554, 759
FaeI CATG 2 cut(s) 127, 207
FaiI YATR 8 cut(s) 98, 125, 165, 205, 516, 645, 744, 776
FaqI GGGAC 1 cut(s) 572
FatI CATG 2 cut(s) 123, 203
Fnu4HI GCNGC 6 cut(s) 118, 224, 294, 297, 371, 725
FokI GGATG 2 cut(s) 416, 662
Fsp4HI GCNGC 6 cut(s) 118, 224, 294, 297, 371, 725
FspBI CTAG 2 cut(s) 572, 705
FspI TGCGCA 1 cut(s) 612
GlaI GCGC 1 cut(s) 612
GluI GCNGC 6 cut(s) 118, 224, 294, 297, 371, 725
GsaI CCCAGC 1 cut(s) 500
HaeIII GGCC 1 cut(s) 304
HapII CCGG 1 cut(s) 401
HgaI GACGC 1 cut(s) 253
HhaI GCGC 1 cut(s) 613
Hin1I GRCGYC 2 cut(s) 264, 752
Hin1II CATG 2 cut(s) 127, 207
Hin6I GCGC 1 cut(s) 611
HinP1I GCGC 1 cut(s) 611
HinfI GANTC 4 cut(s) 57, 565, 670, 735
HpaII CCGG 1 cut(s) 401
HphI GGTGA 4 cut(s) 57, 200, 404, 646
Hpy166II GTNNAC 1 cut(s) 459
Hpy188I TCNGA 3 cut(s) 352, 669, 750
Hpy188III TCNNGA 1 cut(s) 107
Hpy8I GTNNAC 1 cut(s) 459
HpyAV CCTTC 4 cut(s) 330, 481, 519, 674
HpyCH4III ACNGT 3 cut(s) 440, 484, 619
HpyCH4IV ACGT 1 cut(s) 752
HpyCH4V TGCA 5 cut(s) 127, 434, 443, 465, 724
HpyF10VI GCNNNNNNNGC 2 cut(s) 293, 440
HpySE526I ACGT 1 cut(s) 752
Hsp92I GRCGYC 2 cut(s) 264, 752
Hsp92II CATG 2 cut(s) 127, 207
HspAI GCGC 1 cut(s) 611
KpnI GGTACC 1 cut(s) 401
Kzo9I GATC 3 cut(s) 157, 236, 247
LguI GCTCTTC 1 cut(s) 9
LmnI GCTCC 2 cut(s) 256, 778
Lsp1109I GCAGC 4 cut(s) 104, 210, 280, 736
LweI GCATC 1 cut(s) 349
MaeI CTAG 2 cut(s) 572, 705
MaeII ACGT 1 cut(s) 752
MaeIII GTNAC 1 cut(s) 206
MalI GATC 3 cut(s) 159, 238, 249
MboI GATC 3 cut(s) 157, 236, 247
MboII GAAGA 7 cut(s) 26, 49, 69, 72, 259, 618, 621
MflI RGATCY 1 cut(s) 247
MluCI AATT 4 cut(s) 240, 321, 467, 675
MlyI GAGTC 2 cut(s) 664, 744
MnlI CCTC 7 cut(s) 131, 152, 222, 383, 388, 425, 677
MseI TTAA 2 cut(s) 243, 479
MslI CAYNNNNRTG 1 cut(s) 519
MspA1I CMGCKG 1 cut(s) 287
MspI CCGG 1 cut(s) 401
MspR9I CCNGG 3 cut(s) 176, 261, 306
MvaI CCWGG 3 cut(s) 176, 261, 306
MvnI CGCG 1 cut(s) 429
MwoI GCNNNNNNNGC 2 cut(s) 293, 440
NdeII GATC 3 cut(s) 157, 236, 247
NlaIII CATG 2 cut(s) 127, 207
NlaIV GGNNCC 4 cut(s) 258, 399, 588, 688
NmuCI GTSAC 1 cut(s) 206
NsbI TGCGCA 1 cut(s) 612
NspI RCATGY 1 cut(s) 207
PaqCI CACCTGC 1 cut(s) 470
PceI AGGCCT 1 cut(s) 304
PciSI GCTCTTC 1 cut(s) 9
PfeI GAWTC 2 cut(s) 57, 565
PkrI GCNGC 6 cut(s) 119, 225, 295, 298, 372, 726
PleI GAGTC 2 cut(s) 664, 743
PpsI GAGTC 2 cut(s) 664, 743
PsiI TTATAA 2 cut(s) 98, 645
Psp6I CCWGG 3 cut(s) 174, 259, 304
PspFI CCCAGC 1 cut(s) 496
PspGI CCWGG 3 cut(s) 174, 259, 304
PspN4I GGNNCC 4 cut(s) 258, 399, 588, 688
PspPI GGNCC 1 cut(s) 586
PsuI RGATCY 1 cut(s) 247
RsaI GTAC 3 cut(s) 399, 601, 732
RsaNI GTAC 3 cut(s) 398, 600, 731
RseI CAYNNNNRTG 1 cut(s) 519
SapI GCTCTTC 1 cut(s) 9
SaqAI TTAA 2 cut(s) 243, 479
SatI GCNGC 6 cut(s) 118, 224, 294, 297, 371, 725
Sau3AI GATC 3 cut(s) 157, 236, 247
Sau96I GGNCC 1 cut(s) 586
SchI GAGTC 2 cut(s) 664, 744
ScrFI CCNGG 3 cut(s) 176, 261, 306
SfaNI GCATC 1 cut(s) 349
SinI GGWCC 1 cut(s) 586
SmiMI CAYNNNNRTG 1 cut(s) 519
SpeI ACTAGT 1 cut(s) 704
Sse9I AATT 4 cut(s) 240, 321, 467, 675
SseBI AGGCCT 1 cut(s) 304
SsiI CCGC 5 cut(s) 171, 287, 297, 371, 429
SspMI CTAG 2 cut(s) 572, 705
StuI AGGCCT 1 cut(s) 304
StyD4I CCNGG 3 cut(s) 174, 259, 304
StyI CCWWGG 1 cut(s) 690
TaaI ACNGT 3 cut(s) 440, 484, 619
TaiI ACGT 1 cut(s) 755
TaqI TCGA 1 cut(s) 673
TaqII GACCGA 1 cut(s) 574
TasI AATT 4 cut(s) 240, 321, 467, 675
TatI WGTACW 1 cut(s) 599
TauI GCSGC 2 cut(s) 299, 373
TfiI GAWTC 2 cut(s) 57, 565
Tru1I TTAA 2 cut(s) 243, 479
Tru9I TTAA 2 cut(s) 243, 479
TscAI CASTG 2 cut(s) 445, 560
TseFI GTSAC 1 cut(s) 206
TseI GCWGC 4 cut(s) 117, 223, 293, 724
Tsp45I GTSAC 1 cut(s) 206
TspDTI ATGAA 1 cut(s) 503
TspRI CASTG 2 cut(s) 445, 560
VpaK11BI GGWCC 1 cut(s) 586
XceI RCATGY 1 cut(s) 207
XcmI CCANNNNNNNNNTGG 1 cut(s) 466
XspI CTAG 2 cut(s) 572, 705
ZraI GACGTC 1 cut(s) 753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.