Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0012144

Physical Location & Seq

Genomic Coordinates Reverse (-)

20254639 .. 20257916

Gene Structure 3278 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G54720.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2118 bp

ATGTCACAACCTCTCACCACCAGACCCACCGTTACCGGAATATCTATAATCCCTTTCCGACAACCGCCGCCTCTCTGTTCTTTCCTCTTCGTCATCGTTCTTTTCGTCGCCACTTTCTACACTCTCCATCATCCAGACGCCGTTACTCCTCCTCTTCTATTTTCTCGAAATGCCTATAATGCCCTTCGTCTCCGCCGCCTCTTCCTCTCCTCTGCTTCTAACGCCACCATCTCCTCTTACCTCCGTGAATTGACCCGTCATCCTCACCTCGCCGGAACTAAACCCTCTTTAGACACACTTCACTACGTCTTTAACCATTTCCAAAGCCTCGGACTTGAGACCCATGTCGCCGAATACGAAGCTCTCTTATCTTATCCTACGCACATCTCCGTCACGGCAAGTTTTAGCAACACGACGACTCTTGAGTTTGACTTAAACGACGTTCCCGGAGACTCTCCGGTCGTTAGGCCTTACCACGCTTATTCTCCATCTGGGTCGGCGCAAGGTAACGTGGTTTTCGTGAATCACGGCGAGGAGAGAGACTATCATGCGCTTGAGTCTATTGGTGTGAGCGTTAAAGGGTGTGTGGTTTTAGCGAGGAAAGGTGAAAACTTGGGAAGAGGAGCAATTGTGAAAATAGCAGAAGCTAAAGGTGCGCTGGGAGTGCTAATATACGCCGAGAATGACGGTGGTGGATTTGGCGGGATTGAGAGAGGTACGGTTATGAGAGGAATCGGAGATCCGGTTAGCCCGGGTTGGCCCGGAGTTGTCGGAGGAGAAAAACTGAGCTTGGATGACGAATTGGTCACTCGGAGATTTCCCAAAATCCCATCTTTGCCTTTGTCTCTTCGTAATGCTGAGATCATTTTGGCTTCTCTGGGCGGTGCTAGAGCTCCGTTGGAGTGGCGGAATTCGGGTCGTGTCGGACCGGGTCAACGGGTTGGCCCTGGACGGATGGTTATTAACATGACGTTTCAGGGGGAGATGAAAATGAAGAAAATTAACAATGTTGTGGTTACAATAAGAGGGAGTGAAGAAGCGGATAGGTATGTGATACTTGGGAACCATAGAGATGCATGGACATATGGAGCTGTTGATCCAAACAGTGGAACTTCTGCTTTACTTGACATTAGTCGACGATTTGCTCTTTTGCTTAAATCTGGTTGGAGACCACGTAGGACCATTCTCCTTTGTAGTTGGGATGCAGAAGAATTTGGAATGATTGGATCAACTGAATGGATTGAAGAAAATGTTCTTAACTTAGGTGCAAGTGCTGTTGCTTATCTTAATGTAGATTGTGCAGTCCAAGGCTCTGGTTTTTTTGCTGGTGCAACTCCTCAATTAGACGGTCTTCTTGTCGATGTCTTGAAGCTGGTACAGGATCCTGATGCAGTAGGCTTGACAGTGGAAGAGACATTTAAGTCTCAAAATAACATTATTCAGAGGCTAAGTAGAGTAGATTCTGATTTTTCCGGTTTTCTTCATCATGCCGGGATACCGTCTATAGACATGTACTATGGAGCAGATTATCCTGTCTATCACACTGCATTTGACTCCTATGATTGGATGATCCACAACGCAGATCCATTGTTTCACCGTCATGTTGCTATGGCTGGAATTTGGGGACTTTTAGGAATTCTCTTGGCTGATGAGCCACTCATACCATTCGATTACATCTCTTATGCAGACCAATTGCAGGCACATAGAGATAAACTGAGCAAGCTCTTAGAAGGGAAAGTCTCTGTAAATCCCTTAAGCATGGCGATACAAGAGTTCTCTTTGGTTGCCAAAGAAGCCGCAGATGAAGCAAAGAAGTTAAAGGGAAAATCATATAGCAAAAATGATGTAGCAGCAGCGGCGAAGAGAAGAGAGCTAAATGACAGGTTGATGCTTGTAGAAAGAGGGTTTTTGGATGCAGAAGGGATCAAAGGGAAGGAATGGTTCAAGCATCTTGTGTATGGACCGGCGGCTGAGCCAGAGAGCAAGCTAGGATTCTTCCCGGGAATAGCAGATGCCATTGCGATGAACGCATCAGAGGGAATAATTGAGCACGAGATATGGAGAGTCGCAAGAGCGATTCAGAGAGCAAGCAAAGCTCTTAAAGGAGGTTTCACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0004180 GO:0004181 GO:0005575 GO:0006508 GO:0006807 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0008233 GO:0008235 GO:0008237 GO:0008238 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009640 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009908 GO:0009909 GO:0010015 GO:0010051 GO:0010073 GO:0010074 GO:0010075 GO:0010080 GO:0010081 GO:0010082 GO:0010154 GO:0010305 GO:0016020 GO:0016021 GO:0016787 GO:0019538 GO:0019827 GO:0022414 GO:0022622 GO:0031224 GO:0032501 GO:0032502 GO:0040008 GO:0043170 GO:0044238 GO:0044425 GO:0048316 GO:0048364 GO:0048367 GO:0048507 GO:0048509 GO:0048580 GO:0048608 GO:0048638 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050896 GO:0051239 GO:0061458 GO:0065007 GO:0070011 GO:0071704 GO:0080050 GO:0090567 GO:0098727 GO:0099402 GO:0140096 GO:1901564 GO:1905392 GO:2000026 GO:2000034 GO:2000241 GO:2000280

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PA Peptidase_M28 TFR_dimer

Protein Analysis

705

Amino Acids

77.15

Weight (kDa)

6.41

Isoelectric Point (pI)

32.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M28	PF04389	336 - 522	6.5e-23	Peptidase family M28
TFR_dimer	PF04253	580 - 701	8.7e-28	Transferrin receptor-like dimerisation domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012144)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G54720
fragaria_vesca	FvH4_6g21160 FvH4_6g21160 FvH4_6g21160
malus_domestica	MD12G1047100.v1.1 MD14G1046000.v1.1
prunus_persica	Prupe.7G088100_v2.0.a1
pyrus_communis	pycom12g04070 pycom14g03860
rosa_chinensis	RchiOBHm_Chr3g0477621
rosa_laevigata	RLG00000023705
rosa_multiflora	Rmu_sc0000775.1_g000003
rosa_roxburghii	Rroxscaffold_6G00403800
rosa_rugosa	Rorug03G0162500
rosa_samantha	Rh3AG212400 Rh3BG246100 Rh3CG239700 Rh3DG239200
rosa_wichuraiana	Rw3G019230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	3 cut(s)	458, 803, 1420
AccB7I	CCANNNNNTGG	1 cut(s)	1106
AccI	GTMKAC	1 cut(s)	1135
AclWI	GGATC	8 cut(s)	734, 1091, 1234, 1376, 1389, 1564, 1577, 1931
AcsI	RAATTY	4 cut(s)	910, 1211, 1617, 1635
AcyI	GRCGYC	1 cut(s)	138
AfaI	GTAC	3 cut(s)	718, 1377, 1514
AfiI	CCNNNNNNNGG	3 cut(s)	1106, 1563, 1696
AflII	CTTAAG	1 cut(s)	1753
AflIII	ACRYGT	1 cut(s)	1509
AgsI	TTSAA	3 cut(s)	1244, 1369, 1945
AjnI	CCWGG	1 cut(s)	946
Alw21I	GWGCWC	2 cut(s)	895, 2052
Alw26I	GTCTC	9 cut(s)	194, 332, 444, 534, 849, 1162, 1406, 1428, 1744
AlwI	GGATC	8 cut(s)	734, 1091, 1234, 1376, 1389, 1564, 1577, 1931
Ama87I	CYCGRG	2 cut(s)	751, 1999
AoxI	GGCC	3 cut(s)	467, 758, 943
ApeKI	GCWGC	2 cut(s)	1850, 1853
ApoI	RAATTY	4 cut(s)	910, 1211, 1617, 1635
Asp700I	GAANNNNTTC	1 cut(s)	1251
AspLEI	GCGC	3 cut(s)	502, 553, 658
AspS9I	GGNCC	5 cut(s)	759, 926, 944, 1179, 1961
AsuC2I	CCSGG	8 cut(s)	447, 752, 753, 762, 930, 1492, 2000, 2001
AsuHPI	GGTGA	4 cut(s)	7, 257, 617, 1586
AvaI	CYCGRG	2 cut(s)	751, 1999
AvaII	GGWCC	3 cut(s)	926, 1179, 1961
BaeI	ACNNNNGTAYC	2 cut(s)	1757, 1790
BamHI	GGATCC	1 cut(s)	1381
BanII	GRGCYC	1 cut(s)	895
BauI	CACGAG	1 cut(s)	2051
BbsI	GAAGAC	1 cut(s)	1343
Bbv12I	GWGCWC	2 cut(s)	895, 2052
BbvI	GCAGC	2 cut(s)	1862, 1865
BccI	CCATC	5 cut(s)	135, 236, 496, 838, 949
BceAI	ACGGC	3 cut(s)	125, 411, 544
BciT130I	CCWGG	1 cut(s)	948
BciVI	GTATCC	1 cut(s)	1488
BcnI	CCSGG	8 cut(s)	447, 752, 753, 762, 930, 1492, 2000, 2001
BcoDI	GTCTC	9 cut(s)	194, 332, 444, 534, 849, 1162, 1406, 1428, 1744
BfaI	CTAG	2 cut(s)	888, 1988
BfmI	CTRYAG	1 cut(s)	1503
BfrI	CTTAAG	1 cut(s)	1753
BfuI	GTATCC	1 cut(s)	1488
BisI	GCNGC	7 cut(s)	68, 196, 1797, 1851, 1854, 1857, 1968
BlpI	GCTNAGC	1 cut(s)	1971
BlsI	GCNGC	7 cut(s)	69, 197, 1798, 1852, 1855, 1858, 1969
Bme1390I	CCNGG	9 cut(s)	447, 752, 753, 762, 930, 948, 1492, 2000, 2001
Bme18I	GGWCC	3 cut(s)	926, 1179, 1961
BmeT110I	CYCGRG	2 cut(s)	751, 1999
BmgT120I	GGNCC	5 cut(s)	759, 926, 944, 1179, 1961
BmiI	GGNNCC	2 cut(s)	1064, 1383
BmrFI	CCNGG	9 cut(s)	447, 752, 753, 762, 930, 948, 1492, 2000, 2001
BmsI	GCATC	8 cut(s)	1063, 1192, 1378, 1878, 1903, 1957, 2002, 2039
BoxI	GACNNNNGTC	2 cut(s)	344, 1131
BpiI	GAAGAC	1 cut(s)	1343
Bpu1102I	GCTNAGC	1 cut(s)	1971
BpuEI	CTTGAG	3 cut(s)	356, 443, 575
BpuMI	CCSGG	8 cut(s)	447, 752, 753, 762, 930, 1492, 2000, 2001
BsaAI	YACGTR	1 cut(s)	1175
BsaHI	GRCGYC	1 cut(s)	138
BsaI	GGTCTC	2 cut(s)	332, 1162
BsaJI	CCNNGG	5 cut(s)	328, 751, 946, 1306, 1999
BsaWI	WCCGGW	4 cut(s)	35, 457, 742, 1472
BsaXI	ACNNNNNCTCC	6 cut(s)	615, 645, 729, 759, 1159, 1189
Bsc4I	CCNNNNNNNGG	3 cut(s)	1106, 1563, 1696
Bse118I	RCCGGY	1 cut(s)	1963
Bse3DI	GCAATG	1 cut(s)	2016
BseBI	CCWGG	1 cut(s)	948
BseDI	CCNNGG	5 cut(s)	328, 751, 946, 1306, 1999
BseGI	GGATG	7 cut(s)	130, 259, 799, 960, 1207, 1572, 1918
BseLI	CCNNNNNNNGG	3 cut(s)	1106, 1563, 1696
BseMI	GCAATG	1 cut(s)	2016
BseMII	CTCAG	4 cut(s)	776, 849, 1706, 1962
BseRI	GAGGAG	8 cut(s)	138, 141, 199, 223, 548, 636, 789, 1326
BseXI	GCAGC	2 cut(s)	1862, 1865
BseYI	CCCAGC	1 cut(s)	658
BsgI	GTGCAG	1 cut(s)	1320
Bsh1285I	CGRYCG	1 cut(s)	462
BshFI	GGCC	3 cut(s)	469, 760, 945
BsiEI	CGRYCG	1 cut(s)	462
BsiHKAI	GWGCWC	2 cut(s)	895, 2052
BsiHKCI	CYCGRG	2 cut(s)	751, 1999
BslFI	GGGAC	1 cut(s)	1638
BslI	CCNNNNNNNGG	3 cut(s)	1106, 1563, 1696
BsmAI	GTCTC	9 cut(s)	194, 332, 444, 534, 849, 1162, 1406, 1428, 1744
BsmBI	CGTCTC	1 cut(s)	194
BsmFI	GGGAC	1 cut(s)	1638
BsnI	GGCC	3 cut(s)	469, 760, 945
Bso31I	GGTCTC	2 cut(s)	332, 1162
BsoBI	CYCGRG	2 cut(s)	751, 1999
Bsp1286I	GDGCHC	2 cut(s)	895, 2052
Bsp143I	GATC	8 cut(s)	739, 861, 1096, 1226, 1381, 1569, 1582, 1923
Bsp1720I	GCTNAGC	1 cut(s)	1971
BspANI	GGCC	3 cut(s)	469, 760, 945
BspCNI	CTCAG	4 cut(s)	777, 850, 1707, 1963
BspLI	GGNNCC	2 cut(s)	1064, 1383
BspPI	GGATC	8 cut(s)	734, 1091, 1234, 1376, 1389, 1564, 1577, 1931
BspTI	CTTAAG	1 cut(s)	1753
BspTNI	GGTCTC	2 cut(s)	332, 1162
BsrDI	GCAATG	1 cut(s)	2016
BsrFI	RCCGGY	1 cut(s)	1963
BssAI	RCCGGY	1 cut(s)	1963
BssECI	CCNNGG	5 cut(s)	328, 751, 946, 1306, 1999
BssMI	GATC	8 cut(s)	739, 861, 1096, 1226, 1381, 1569, 1582, 1923
BssNI	GRCGYC	1 cut(s)	138
BssSI	CACGAG	1 cut(s)	2051
BssT1I	CCWWGG	1 cut(s)	1306
Bst2BI	CACGAG	1 cut(s)	2051
Bst2UI	CCWGG	1 cut(s)	948
Bst4CI	ACNGT	8 cut(s)	31, 689, 721, 1106, 1349, 1405, 1500, 1598
Bst6I	CTCTTC	8 cut(s)	92, 159, 206, 613, 852, 1404, 1856, 1861
BstACI	GRCGYC	1 cut(s)	138
BstAFI	CTTAAG	1 cut(s)	1753
BstBAI	YACGTR	1 cut(s)	1175
BstC8I	GCNNGC	4 cut(s)	1698, 1721, 1985, 2089
BstDEI	CTNAG	7 cut(s)	785, 858, 1261, 1448, 1715, 1726, 1971
BstF5I	GGATG	7 cut(s)	130, 259, 799, 960, 1207, 1572, 1918
BstHHI	GCGC	3 cut(s)	502, 553, 658
BstKTI	GATC	8 cut(s)	742, 864, 1099, 1229, 1384, 1572, 1585, 1926
BstMAI	GTCTC	9 cut(s)	194, 332, 444, 534, 849, 1162, 1406, 1428, 1744
BstMBI	GATC	8 cut(s)	739, 861, 1096, 1226, 1381, 1569, 1582, 1923
BstMCI	CGRYCG	1 cut(s)	462
BstMWI	GCNNNNNNNGC	9 cut(s)	179, 221, 653, 664, 1793, 1805, 1856, 2027, 2093
BstNI	CCWGG	1 cut(s)	948
BstNSI	RCATGY	1 cut(s)	1513
BstPAI	GACNNNNGTC	2 cut(s)	344, 1131
BstSCI	CCNGG	9 cut(s)	445, 750, 751, 760, 928, 946, 1490, 1998, 1999
BstSFI	CTRYAG	1 cut(s)	1503
BstV1I	GCAGC	2 cut(s)	1862, 1865
BstV2I	GAAGAC	1 cut(s)	1343
BstX2I	RGATCY	3 cut(s)	739, 1381, 1582
BstXI	CCANNNNNNTGG	1 cut(s)	1313
BstYI	RGATCY	3 cut(s)	739, 1381, 1582
BsuI	GTATCC	1 cut(s)	1488
BsuRI	GGCC	3 cut(s)	469, 760, 945
BtgZI	GCGATG	1 cut(s)	2036
BtsCI	GGATG	7 cut(s)	130, 259, 799, 960, 1207, 1572, 1918
BtsI	GCAGTG	1 cut(s)	1542
BtsIMutI	CAGTG	3 cut(s)	1111, 1410, 1542
Cac8I	GCNNGC	4 cut(s)	1698, 1721, 1985, 2089
CfoI	GCGC	3 cut(s)	502, 553, 658
Cfr10I	RCCGGY	1 cut(s)	1963
Cfr13I	GGNCC	5 cut(s)	759, 926, 944, 1179, 1961
Cfr9I	CCCGGG	2 cut(s)	751, 1999
CpoI	CGGWCCG	1 cut(s)	926
CseI	GACGC	1 cut(s)	146
Csp6I	GTAC	3 cut(s)	717, 1376, 1513
CspI	CGGWCCG	1 cut(s)	926
CviAII	CATG	9 cut(s)	344, 548, 967, 1077, 1487, 1510, 1601, 1759, 2115
CviQI	GTAC	3 cut(s)	717, 1376, 1513
DdeI	CTNAG	7 cut(s)	785, 858, 1261, 1448, 1715, 1726, 1971
DpnI	GATC	8 cut(s)	741, 863, 1098, 1228, 1383, 1571, 1584, 1925
DpnII	GATC	8 cut(s)	739, 861, 1096, 1226, 1381, 1569, 1582, 1923
DrdI	GACNNNNNNGTC	3 cut(s)	458, 803, 1420
DseDI	GACNNNNNNGTC	3 cut(s)	458, 803, 1420
Eam1104I	CTCTTC	8 cut(s)	92, 159, 206, 613, 852, 1404, 1856, 1861
EarI	CTCTTC	8 cut(s)	92, 159, 206, 613, 852, 1404, 1856, 1861
EciI	GGCGGA	2 cut(s)	182, 922
Ecl136II	GAGCTC	1 cut(s)	893
Eco130I	CCWWGG	1 cut(s)	1306
Eco147I	AGGCCT	1 cut(s)	469
Eco24I	GRGCYC	1 cut(s)	895
Eco31I	GGTCTC	2 cut(s)	332, 1162
Eco47I	GGWCC	3 cut(s)	926, 1179, 1961
Eco53kI	GAGCTC	1 cut(s)	893
Eco88I	CYCGRG	2 cut(s)	751, 1999
EcoICRI	GAGCTC	1 cut(s)	893
EcoRI	GAATTC	2 cut(s)	910, 1635
EcoRII	CCWGG	1 cut(s)	946
EcoT14I	CCWWGG	1 cut(s)	1306
EcoT22I	ATGCAT	1 cut(s)	1078
EcoT38I	GRGCYC	1 cut(s)	895
ErhI	CCWWGG	1 cut(s)	1306
Esp3I	CGTCTC	1 cut(s)	194
FaeI	CATG	9 cut(s)	347, 551, 970, 1080, 1490, 1513, 1604, 1762, 2118
FaqI	GGGAC	1 cut(s)	1638
FatI	CATG	9 cut(s)	343, 547, 966, 1076, 1486, 1509, 1600, 1758, 2114
FauI	CCCGC	1 cut(s)	695
FauNDI	CATATG	1 cut(s)	1084
FblI	GTMKAC	1 cut(s)	1135
Fnu4HI	GCNGC	7 cut(s)	68, 196, 1797, 1851, 1854, 1857, 1968
FokI	GGATG	7 cut(s)	117, 246, 806, 967, 1214, 1579, 1925
FriOI	GRGCYC	1 cut(s)	895
Fsp4HI	GCNGC	7 cut(s)	68, 196, 1797, 1851, 1854, 1857, 1968
FspBI	CTAG	2 cut(s)	888, 1988
GlaI	GCGC	3 cut(s)	501, 552, 657
GluI	GCNGC	7 cut(s)	68, 196, 1797, 1851, 1854, 1857, 1968
GsaI	CCCAGC	1 cut(s)	662
HaeIII	GGCC	3 cut(s)	469, 760, 945
HgaI	GACGC	1 cut(s)	146
HhaI	GCGC	3 cut(s)	502, 553, 658
Hin1I	GRCGYC	1 cut(s)	138
Hin1II	CATG	9 cut(s)	347, 551, 970, 1080, 1490, 1513, 1604, 1762, 2118
Hin6I	GCGC	3 cut(s)	500, 551, 656
HinP1I	GCGC	3 cut(s)	500, 551, 656
HincII	GTYRAC	2 cut(s)	935, 1136
HindII	GTYRAC	2 cut(s)	935, 1136
HphI	GGTGA	4 cut(s)	7, 257, 617, 1586
Hpy166II	GTNNAC	2 cut(s)	935, 1136
Hpy188III	TCNNGA	6 cut(s)	134, 165, 422, 520, 1366, 1385
Hpy8I	GTNNAC	2 cut(s)	935, 1136
Hpy99I	CGWCG	4 cut(s)	110, 418, 443, 1140
HpyAV	CCTTC	4 cut(s)	194, 1724, 1913, 1927
HpyCH4III	ACNGT	8 cut(s)	31, 689, 721, 1106, 1349, 1405, 1500, 1598
HpyCH4IV	ACGT	5 cut(s)	306, 441, 510, 971, 1174
HpyF10VI	GCNNNNNNNGC	9 cut(s)	179, 221, 653, 664, 1793, 1805, 1856, 2027, 2093
HpyF3I	CTNAG	7 cut(s)	785, 858, 1261, 1448, 1715, 1726, 1971
HpySE526I	ACGT	5 cut(s)	306, 441, 510, 971, 1174
Hsp92I	GRCGYC	1 cut(s)	138
Hsp92II	CATG	9 cut(s)	347, 551, 970, 1080, 1490, 1513, 1604, 1762, 2118
HspAI	GCGC	3 cut(s)	500, 551, 656
Kzo9I	GATC	8 cut(s)	739, 861, 1096, 1226, 1381, 1569, 1582, 1923
LmnI	GCTCC	4 cut(s)	623, 898, 1088, 1520
Lsp1109I	GCAGC	2 cut(s)	1862, 1865
LweI	GCATC	8 cut(s)	1063, 1192, 1378, 1878, 1903, 1957, 2002, 2039
MaeI	CTAG	2 cut(s)	888, 1988
MaeII	ACGT	5 cut(s)	306, 441, 510, 971, 1174
MaeIII	GTNAC	7 cut(s)	3, 31, 142, 391, 506, 805, 1015
MalI	GATC	8 cut(s)	741, 863, 1098, 1228, 1383, 1571, 1584, 1925
MboI	GATC	8 cut(s)	739, 861, 1096, 1226, 1381, 1569, 1582, 1923
MfeI	CAATTG	2 cut(s)	627, 1691
MflI	RGATCY	3 cut(s)	739, 1381, 1582
MhlI	GDGCHC	2 cut(s)	895, 2052
MlyI	GAGTC	5 cut(s)	412, 446, 566, 1547, 2073
MmeI	TCCRAC	5 cut(s)	82, 751, 879, 904, 1145
Mph1103I	ATGCAT	1 cut(s)	1078
MroXI	GAANNNNTTC	1 cut(s)	1251
MslI	CAYNNNNRTG	3 cut(s)	1071, 1599, 2021
MspA1I	CMGCKG	1 cut(s)	1856
MspCI	CTTAAG	1 cut(s)	1753
MspR9I	CCNGG	9 cut(s)	447, 752, 753, 762, 930, 948, 1492, 2000, 2001
MunI	CAATTG	2 cut(s)	627, 1691
MvaI	CCWGG	1 cut(s)	948
MwoI	GCNNNNNNNGC	9 cut(s)	179, 221, 653, 664, 1793, 1805, 1856, 2027, 2093
NciI	CCSGG	8 cut(s)	447, 752, 753, 762, 930, 1492, 2000, 2001
NdeI	CATATG	1 cut(s)	1084
NdeII	GATC	8 cut(s)	739, 861, 1096, 1226, 1381, 1569, 1582, 1923
NlaIII	CATG	9 cut(s)	347, 551, 970, 1080, 1490, 1513, 1604, 1762, 2118
NlaIV	GGNNCC	2 cut(s)	1064, 1383
NmeAIII	GCCGAG	1 cut(s)	703
NmuCI	GTSAC	3 cut(s)	3, 391, 805
NsiI	ATGCAT	1 cut(s)	1078
NspI	RCATGY	1 cut(s)	1513
PceI	AGGCCT	1 cut(s)	469
PciI	ACATGT	1 cut(s)	1509
PcsI	WCGNNNNNNNCGW	3 cut(s)	102, 354, 516
PdmI	GAANNNNTTC	1 cut(s)	1251
PfeI	GAWTC	5 cut(s)	523, 732, 1460, 1992, 2077
PflFI	GACNNNGTC	1 cut(s)	930
PflMI	CCANNNNNTGG	1 cut(s)	1106
PfoI	TCCNGGA	1 cut(s)	445
PkrI	GCNGC	7 cut(s)	69, 197, 1798, 1852, 1855, 1858, 1969
PleI	GAGTC	5 cut(s)	412, 446, 565, 1547, 2072
PpsI	GAGTC	5 cut(s)	412, 446, 565, 1547, 2072
Ppu21I	YACGTR	1 cut(s)	1175
PscI	ACATGT	1 cut(s)	1509
PshAI	GACNNNNGTC	2 cut(s)	344, 1131
Psp124BI	GAGCTC	1 cut(s)	895
Psp6I	CCWGG	1 cut(s)	946
PspFI	CCCAGC	1 cut(s)	658
PspGI	CCWGG	1 cut(s)	946
PspN4I	GGNNCC	2 cut(s)	1064, 1383
PspPI	GGNCC	5 cut(s)	759, 926, 944, 1179, 1961
PsuI	RGATCY	3 cut(s)	739, 1381, 1582
PsyI	GACNNNGTC	1 cut(s)	930
RsaI	GTAC	3 cut(s)	718, 1377, 1514
RsaNI	GTAC	3 cut(s)	717, 1376, 1513
RseI	CAYNNNNRTG	3 cut(s)	1071, 1599, 2021
Rsr2I	CGGWCCG	1 cut(s)	926
RsrII	CGGWCCG	1 cut(s)	926
SacI	GAGCTC	1 cut(s)	895
SalI	GTCGAC	1 cut(s)	1134
SatI	GCNGC	7 cut(s)	68, 196, 1797, 1851, 1854, 1857, 1968
Sau3AI	GATC	8 cut(s)	739, 861, 1096, 1226, 1381, 1569, 1582, 1923
Sau96I	GGNCC	5 cut(s)	759, 926, 944, 1179, 1961
SchI	GAGTC	5 cut(s)	412, 446, 566, 1547, 2073
ScrFI	CCNGG	9 cut(s)	447, 752, 753, 762, 930, 948, 1492, 2000, 2001
SduI	GDGCHC	2 cut(s)	895, 2052
SfaNI	GCATC	8 cut(s)	1063, 1192, 1378, 1878, 1903, 1957, 2002, 2039
SfcI	CTRYAG	1 cut(s)	1503
SinI	GGWCC	3 cut(s)	926, 1179, 1961
SmaI	CCCGGG	2 cut(s)	753, 2001
SmiMI	CAYNNNNRTG	3 cut(s)	1071, 1599, 2021
SmlI	CTYRAG	4 cut(s)	335, 422, 554, 1753
SmoI	CTYRAG	4 cut(s)	335, 422, 554, 1753
SseBI	AGGCCT	1 cut(s)	469
SspMI	CTAG	2 cut(s)	888, 1988
SstI	GAGCTC	1 cut(s)	895
StuI	AGGCCT	1 cut(s)	469
StyD4I	CCNGG	9 cut(s)	445, 750, 751, 760, 928, 946, 1490, 1998, 1999
StyI	CCWWGG	1 cut(s)	1306
TaaI	ACNGT	8 cut(s)	31, 689, 721, 1106, 1349, 1405, 1500, 1598
TaiI	ACGT	5 cut(s)	309, 444, 513, 974, 1177
TaqI	TCGA	4 cut(s)	166, 1135, 1359, 1668
TatI	WGTACW	1 cut(s)	1512
TauI	GCSGC	5 cut(s)	70, 198, 1799, 1859, 1970
TfiI	GAWTC	5 cut(s)	523, 732, 1460, 1992, 2077
TscAI	CASTG	3 cut(s)	1111, 1410, 1549
TseFI	GTSAC	3 cut(s)	3, 391, 805
TseI	GCWGC	2 cut(s)	1850, 1853
Tsp45I	GTSAC	3 cut(s)	3, 391, 805
TspDTI	ATGAA	5 cut(s)	1001, 1007, 1472, 1818, 2039
TspGWI	ACGGA	4 cut(s)	233, 379, 885, 967
TspMI	CCCGGG	2 cut(s)	751, 1999
TspRI	CASTG	3 cut(s)	1111, 1410, 1549
Tth111I	GACNNNGTC	1 cut(s)	930
Van91I	CCANNNNNTGG	1 cut(s)	1106
Vha464I	CTTAAG	1 cut(s)	1753
VpaK11BI	GGWCC	3 cut(s)	926, 1179, 1961
XapI	RAATTY	4 cut(s)	910, 1211, 1617, 1635
XceI	RCATGY	1 cut(s)	1513
XmaI	CCCGGG	2 cut(s)	751, 1999
XmiI	GTMKAC	1 cut(s)	1135
XmnI	GAANNNNTTC	1 cut(s)	1251
XspI	CTAG	2 cut(s)	888, 1988
Zsp2I	ATGCAT	1 cut(s)	1078

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.