Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0012144

Physical Location & Seq

Genomic Coordinates Forward (+)

5424172 .. 5428910

Gene Structure 4739 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1047100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2127 bp

ATGGGTCAACTCTTCCCCAACTCCACCACCCTCTTCACTTCAAAGGTCTCACCCACATGCACCTTCGTCTCCCTCCTCATCGTCGGTATTCTGGGTTTTTATACCCTGCATTCCCCCCAGCCTGCACTCTCCTCCTCAAATTCCCACTCTGCCCCCCTATTCCGCCAGATTTTCCTCTCCCACGCCACCAACGCCACCCTCTCCTCCTACCTCCGCGCCCTCACCCTCCACCCCCACCTCGCCGGCACCCCGCCCTCCCTCGACACCGCCAACTTCGTCCGGGCCCACTTCCTGGGCCTGAGACTCGACACCCGGTCGGCCCACTACAACGCCCTCCTATCCTACCCACTCCACTCCTCACTCTCCGCGCATTTTAGCAACGGCTCCCGCGTGGACATCCCGTTAACCGAGACCGGCCTTAACAATGACCACGACGGTGTCGTTCCGCCGTACCACGCCTACTCCCCCACTGGATCGGCGCACGCTAAGCTGGCCTTCGCCAACCACGGCACGGACGAGGACTATCGTGCGCTGGGGGAGCTCGGGGTGAACGTCAGCGGGTGCGTGGTGATTGTTAGAAGAGGCGATGACGTGTCCAGAGGCGAAGTGGTCCGGAAGGCCGAGCAAAACGGTGCGCTGGCTGTGCTATTGTACACCGAGGGGGGAGATGGTGATGCAAGTGGGGGGTTTAAGAAGGGTTTTGAGAGAGGGTTTGTGATGAACGGCGTGGGGGACCCACTGAGTCCCGGGTGGGCCGGGGTCGACGGAGCCGAGAGGCTGGACCTGGACGACCCTGAGGTTTTGAAGAAGTTTCCGAAAATTCCATCCATGCCGCTGTCCGCGGAGGCCGCCGAGACCCTTCTGGGGTGGCTCGGAGGGGCTCCCGTGCCGCCAGCGTGGAGGGAATCGCTGCCCGCGGCAGTGGGCGGTGTCGGGCCTGGACCGACAGTGGTGAATTTCACTTACCAGGGGGAGAAAAAGGTGGTGAAGATTCACAATGTTTTTGCTGTCATAAGAGGTTCAGAAGAGCCCGACCGCTATGTGCTGCTTGGCAACCATAGAGATGCGTGGACGTTTGGGGCTGTTGACCCCAATAGTGGAACTGCAGCATTACTTGACATTGCTCGTAGATATTCTCTTTTGATGCGTTCGGGTTGGAATCCTAGGAGGACGATTATTCTCTGTAGTTGGGATGCAGAAGAGTTTGGAATGGTAGGATCTACTGAGTGGGTTGAACAGAACATTGCTAATCTGGGTTCCAAAGCCGTTGCCTACCTCAATGTAGATTGTGCGGTTCAAGGGCCAGGGTTCTTTGTTCGAGCAACTCCTCAGCTGGACAATATCATCCTTGAGGTTACAAAGATGGTTAAAGATCCTGACTCTGTCGGTGCCACTGTATATGAAAAATGGATGACAGAAAATAGAAGTGTTGATATCCAAAGACTCAGTGCTGTGGATTCTGATTTTGCCCCATTTTTGCAACATGCAGGGATTCCTTCCACGGATATGTACTATGGAATTGATTTTCCTGTTTATCACACTGCATTTGATGCCTATGAGTGGATGACAAACTTTGGAGATCCTTTGTTTCAGAGACATGTGGCTGTTGCTGGAATGTGGGGACTTCTAGCCCTCCACCTGGCCGACGATTCAATTATACCTTTCAGTTTCCTCTCTTATGCGGATCAGTTAAAGCATTATAAAGATGTTTTGAGCAACTTGTTAGACGGGGGTGTATCTCTACATCCCCTCACAACAGCTATTCAGGAATTTGCCTATGCCGCCAAAGAAGCTGAAGATGAAGCACAGAAGCTGAGAGAGAAAGAAAATACAAGTGAGCTTGTAGTTTTGAAGAAGCGGGCACTGAATGATCGGCTGATGCTTGCTGAAAGGGGCTTCCTGGATTCAGATGGACTTGAAGGAAGACAGTGGCTCAAGCATCTTGTGTATGGGCCTCCTGGTGAGCATGGAAGCAAACTATCCTTCTTTCCCGGAGTAGGCGATGCAATGCTTCGAGCAAAGAGAATGAGCAGGAGAGAAGGGCAGGCCAAAATTCAGCACGAGATCTGGAGAGTTGCCAGAGCCATACAGAGGGCTGCAAATTCCCTGAGAGGAGACGTTTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0004180 GO:0004181 GO:0005575 GO:0006508 GO:0006807 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0008233 GO:0008235 GO:0008237 GO:0008238 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009640 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009908 GO:0009909 GO:0010015 GO:0010051 GO:0010073 GO:0010074 GO:0010075 GO:0010080 GO:0010081 GO:0010082 GO:0010154 GO:0010305 GO:0016020 GO:0016021 GO:0016787 GO:0019538 GO:0019827 GO:0022414 GO:0022622 GO:0031224 GO:0032501 GO:0032502 GO:0040008 GO:0043170 GO:0044238 GO:0044425 GO:0048316 GO:0048364 GO:0048367 GO:0048507 GO:0048509 GO:0048580 GO:0048608 GO:0048638 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050896 GO:0051239 GO:0061458 GO:0065007 GO:0070011 GO:0071704 GO:0080050 GO:0090567 GO:0098727 GO:0099402 GO:0140096 GO:1901564 GO:1905392 GO:2000026 GO:2000034 GO:2000241 GO:2000280

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PA Peptidase_M28 TFR_dimer

Protein Analysis

709

Amino Acids

77.31

Weight (kDa)

6.0

Isoelectric Point (pI)

32.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PA	PF02225	166 - 227	4.3e-06	PA domain
Peptidase_M28	PF04389	333 - 520	9e-23	Peptidase family M28
TFR_dimer	PF04253	579 - 704	1.4e-29	Transferrin receptor-like dimerisation domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012144)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G54720
fragaria_vesca	FvH4_6g21160 FvH4_6g21160 FvH4_6g21160
malus_domestica	MD12G1047100.v1.1 MD14G1046000.v1.1
prunus_persica	Prupe.7G088100_v2.0.a1
pyrus_communis	pycom12g04070 pycom14g03860
rosa_chinensis	RchiOBHm_Chr3g0477621
rosa_laevigata	RLG00000023705
rosa_multiflora	Rmu_sc0000775.1_g000003
rosa_roxburghii	Rroxscaffold_6G00403800
rosa_rugosa	Rorug03G0162500
rosa_samantha	Rh3AG212400 Rh3BG246100 Rh3CG239700 Rh3DG239200
rosa_wichuraiana	Rw3G019230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1701
AccB1I	GGYRCC	2 cut(s)	245, 1388
AccB7I	CCANNNNNTGG	1 cut(s)	292
AccI	GTMKAC	1 cut(s)	762
AccII	CGCG	5 cut(s)	216, 368, 390, 842, 917
AccIII	TCCGGA	1 cut(s)	612
AclWI	GGATC	5 cut(s)	481, 1225, 1367, 1574, 1692
AcoI	YGGCCR	1 cut(s)	1641
AcsI	RAATTY	6 cut(s)	139, 819, 955, 1769, 2052, 2101
AcuI	CTGAAG	1 cut(s)	1815
AfaI	GTAC	3 cut(s)	452, 653, 1511
AfiI	CCNNNNNNNGG	8 cut(s)	292, 511, 864, 1097, 1560, 1639, 1997, 2091
AflIII	ACRYGT	2 cut(s)	591, 1597
AgsI	TTSAA	7 cut(s)	42, 805, 1235, 1298, 1653, 1852, 1919
AhdI	GACNNNNNGTC	1 cut(s)	313
AjiI	CACGTC	1 cut(s)	592
AjnI	CCWGG	8 cut(s)	291, 783, 937, 966, 1303, 1638, 1899, 1957
AleI	CACNNNNGTG	1 cut(s)	435
AluBI	AGCT	7 cut(s)	490, 541, 1333, 1760, 1793, 1813, 1840
AluI	AGCT	7 cut(s)	490, 541, 1333, 1760, 1793, 1813, 1840
Alw21I	GWGCWC	1 cut(s)	543
Alw26I	GTCTC	7 cut(s)	52, 73, 295, 404, 848, 1588, 2109
AlwI	GGATC	5 cut(s)	481, 1225, 1367, 1574, 1692
AlwNI	CAGNNNCTG	1 cut(s)	1813
Ama87I	CYCGRG	2 cut(s)	542, 746
Aor13HI	TCCGGA	1 cut(s)	612
ApaI	GGGCCC	1 cut(s)	286
ApeKI	GCWGC	4 cut(s)	910, 1045, 1106, 2096
ApoI	RAATTY	6 cut(s)	139, 819, 955, 1769, 2052, 2101
AspA2I	CCTAGG	1 cut(s)	1163
AspLEI	GCGC	5 cut(s)	218, 370, 481, 532, 637
AsuC2I	CCSGG	6 cut(s)	281, 313, 747, 748, 757, 1992
AsuHPI	GGTGA	8 cut(s)	42, 214, 559, 580, 683, 964, 997, 1973
AvaI	CYCGRG	2 cut(s)	542, 746
AvaII	GGWCC	4 cut(s)	610, 733, 781, 941
AvrII	CCTAGG	1 cut(s)	1163
AxyI	CCTNAGG	1 cut(s)	795
BaeGI	GKGCMC	2 cut(s)	286, 1864
BanI	GGYRCC	2 cut(s)	245, 1388
BanII	GRGCYC	4 cut(s)	286, 543, 883, 1032
BauI	CACGAG	1 cut(s)	2060
BbsI	GAAGAC	1 cut(s)	1930
Bbv12I	GWGCWC	1 cut(s)	543
BbvCI	CCTCAGC	1 cut(s)	1329
BbvI	GCAGC	4 cut(s)	897, 1032, 1118, 2083
BccI	CCATC	4 cut(s)	662, 832, 1357, 1904
BceAI	ACGGC	5 cut(s)	397, 433, 523, 739, 1250
BciT130I	CCWGG	8 cut(s)	293, 785, 939, 968, 1305, 1640, 1901, 1959
BcnI	CCSGG	6 cut(s)	281, 313, 747, 748, 757, 1992
BcoDI	GTCTC	7 cut(s)	52, 73, 295, 404, 848, 1588, 2109
BfaI	CTAG	2 cut(s)	1164, 1628
BfmI	CTRYAG	2 cut(s)	1104, 1183
BglII	AGATCT	1 cut(s)	2064
BisI	GCNGC	9 cut(s)	833, 849, 890, 911, 918, 1046, 1107, 1782, 2097
BlnI	CCTAGG	1 cut(s)	1163
BlpI	GCTNAGC	1 cut(s)	486
BlsI	GCNGC	9 cut(s)	834, 850, 891, 912, 919, 1047, 1108, 1783, 2098
Bme18I	GGWCC	4 cut(s)	610, 733, 781, 941
BmeRI	GACNNNNNGTC	1 cut(s)	313
BmeT110I	CYCGRG	2 cut(s)	542, 746
BmgBI	CACGTC	1 cut(s)	592
BmiI	GGNNCC	9 cut(s)	247, 284, 385, 734, 735, 769, 882, 1258, 1390
BmsI	GCATC	8 cut(s)	664, 1054, 1134, 1183, 1540, 1869, 1948, 1993
BpiI	GAAGAC	1 cut(s)	1930
BpmI	CTGGAG	1 cut(s)	2089
Bpu10I	CCTNAGC	1 cut(s)	1329
Bpu1102I	GCTNAGC	1 cut(s)	486
BpuEI	CTTGAG	2 cut(s)	1370, 1919
BpuMI	CCSGG	6 cut(s)	281, 313, 747, 748, 757, 1992
BsaI	GGTCTC	3 cut(s)	52, 404, 848
BsaWI	WCCGGW	1 cut(s)	612
BsaXI	ACNNNNNCTCC	2 cut(s)	446, 476
Bsc4I	CCNNNNNNNGG	8 cut(s)	292, 511, 864, 1097, 1560, 1639, 1997, 2091
Bse118I	RCCGGY	2 cut(s)	242, 413
Bse1I	ACTGG	1 cut(s)	475
Bse21I	CCTNAGG	1 cut(s)	795
Bse3DI	GCAATG	3 cut(s)	1119, 1242, 2013
BseAI	TCCGGA	1 cut(s)	612
BseBI	CCWGG	8 cut(s)	293, 785, 939, 968, 1305, 1640, 1901, 1959
BseGI	GGATG	7 cut(s)	396, 824, 1198, 1344, 1416, 1569, 1744
BseLI	CCNNNNNNNGG	8 cut(s)	292, 511, 864, 1097, 1560, 1639, 1997, 2091
BseMI	GCAATG	3 cut(s)	1119, 1242, 2013
BseMII	CTCAG	8 cut(s)	290, 731, 786, 1215, 1343, 1459, 1805, 2099
BseNI	ACTGG	1 cut(s)	475
BseRI	GAGGAG	7 cut(s)	65, 121, 124, 193, 346, 1317, 2127
BseSI	GKGCMC	2 cut(s)	286, 1864
BseXI	GCAGC	4 cut(s)	897, 1032, 1118, 2083
BseYI	CCCAGC	2 cut(s)	117, 532
BsgI	GTGCAG	1 cut(s)	108
Bsh1236I	CGCG	5 cut(s)	216, 368, 390, 842, 917
Bsh1285I	CGRYCG	2 cut(s)	317, 1036
BshNI	GGYRCC	2 cut(s)	245, 1388
BsiEI	CGRYCG	2 cut(s)	317, 1036
BsiHKAI	GWGCWC	1 cut(s)	543
BsiHKCI	CYCGRG	2 cut(s)	542, 746
BsiSI	CCGG	8 cut(s)	243, 280, 313, 414, 613, 747, 756, 1992
BslFI	GGGAC	3 cut(s)	729, 746, 1635
BslI	CCNNNNNNNGG	8 cut(s)	292, 511, 864, 1097, 1560, 1639, 1997, 2091
BsmAI	GTCTC	7 cut(s)	52, 73, 295, 404, 848, 1588, 2109
BsmBI	CGTCTC	2 cut(s)	73, 2109
BsmFI	GGGAC	3 cut(s)	729, 746, 1635
BsmI	GAATGC	1 cut(s)	109
Bso31I	GGTCTC	3 cut(s)	52, 404, 848
BsoBI	CYCGRG	2 cut(s)	542, 746
Bsp120I	GGGCCC	1 cut(s)	282
Bsp1286I	GDGCHC	5 cut(s)	286, 543, 883, 1032, 1864
Bsp13I	TCCGGA	1 cut(s)	612
Bsp1407I	TGTACA	1 cut(s)	651
Bsp143I	GATC	7 cut(s)	473, 1217, 1372, 1579, 1684, 1870, 2064
Bsp1720I	GCTNAGC	1 cut(s)	486
BspCNI	CTCAG	8 cut(s)	291, 732, 787, 1216, 1342, 1458, 1806, 2100
BspEI	TCCGGA	1 cut(s)	612
BspFNI	CGCG	5 cut(s)	216, 368, 390, 842, 917
BspLI	GGNNCC	9 cut(s)	247, 284, 385, 734, 735, 769, 882, 1258, 1390
BspMAI	CTGCAG	1 cut(s)	1108
BspPI	GGATC	5 cut(s)	481, 1225, 1367, 1574, 1692
BspQI	GCTCTTC	1 cut(s)	1020
BspT107I	GGYRCC	2 cut(s)	245, 1388
BspTNI	GGTCTC	3 cut(s)	52, 404, 848
BsrDI	GCAATG	3 cut(s)	1119, 1242, 2013
BsrFI	RCCGGY	2 cut(s)	242, 413
BsrGI	TGTACA	1 cut(s)	651
BsrI	ACTGG	1 cut(s)	475
BssAI	RCCGGY	2 cut(s)	242, 413
BssMI	GATC	7 cut(s)	473, 1217, 1372, 1579, 1684, 1870, 2064
BssSI	CACGAG	1 cut(s)	2060
BssT1I	CCWWGG	1 cut(s)	1163
Bst2BI	CACGAG	1 cut(s)	2060
Bst2UI	CCWGG	8 cut(s)	293, 785, 939, 968, 1305, 1640, 1901, 1959
Bst4CI	ACNGT	5 cut(s)	437, 632, 949, 1396, 1929
Bst6I	CTCTTC	5 cut(s)	17, 38, 574, 1020, 1194
BstAPI	GCANNNNNTGC	1 cut(s)	1550
BstAUI	TGTACA	1 cut(s)	651
BstDEI	CTNAG	9 cut(s)	299, 486, 740, 795, 1224, 1329, 1445, 1814, 2108
BstDSI	CCRYGG	4 cut(s)	505, 840, 915, 1500
BstF5I	GGATG	7 cut(s)	396, 824, 1198, 1344, 1416, 1569, 1744
BstFNI	CGCG	5 cut(s)	216, 368, 390, 842, 917
BstHHI	GCGC	5 cut(s)	218, 370, 481, 532, 637
BstKTI	GATC	7 cut(s)	476, 1220, 1375, 1582, 1687, 1873, 2067
BstMAI	GTCTC	7 cut(s)	52, 73, 295, 404, 848, 1588, 2109
BstMBI	GATC	7 cut(s)	473, 1217, 1372, 1579, 1684, 1870, 2064
BstMCI	CGRYCG	2 cut(s)	317, 1036
BstMWI	GCNNNNNNNGC	9 cut(s)	191, 487, 491, 538, 643, 848, 1550, 1781, 1790
BstNI	CCWGG	8 cut(s)	293, 785, 939, 968, 1305, 1640, 1901, 1959
BstNSI	RCATGY	3 cut(s)	60, 1487, 1601
BstSFI	CTRYAG	2 cut(s)	1104, 1183
BstSLI	GKGCMC	2 cut(s)	286, 1864
BstUI	CGCG	5 cut(s)	216, 368, 390, 842, 917
BstV1I	GCAGC	4 cut(s)	897, 1032, 1118, 2083
BstV2I	GAAGAC	1 cut(s)	1930
BstX2I	RGATCY	4 cut(s)	1217, 1372, 1579, 2064
BstYI	RGATCY	4 cut(s)	1217, 1372, 1579, 2064
Bsu36I	CCTNAGG	1 cut(s)	795
BtgI	CCRYGG	4 cut(s)	505, 840, 915, 1500
BtgZI	GCGATG	2 cut(s)	600, 2016
BtrI	CACGTC	1 cut(s)	592
BtsCI	GGATG	7 cut(s)	396, 824, 1198, 1344, 1416, 1569, 1744
BtsI	GCAGTG	2 cut(s)	927, 1539
BtsIMutI	CAGTG	9 cut(s)	468, 737, 927, 954, 1392, 1453, 1539, 1862, 1934
CaiI	CAGNNNCTG	1 cut(s)	1813
CfoI	GCGC	5 cut(s)	218, 370, 481, 532, 637
Cfr10I	RCCGGY	2 cut(s)	242, 413
Cfr42I	CCGCGG	2 cut(s)	843, 918
Cfr9I	CCCGGG	1 cut(s)	746
Csp6I	GTAC	3 cut(s)	451, 652, 1510
CviAII	CATG	5 cut(s)	57, 829, 1484, 1598, 1967
CviQI	GTAC	3 cut(s)	451, 652, 1510
DdeI	CTNAG	9 cut(s)	299, 486, 740, 795, 1224, 1329, 1445, 1814, 2108
DpnI	GATC	7 cut(s)	475, 1219, 1374, 1581, 1686, 1872, 2066
DpnII	GATC	7 cut(s)	473, 1217, 1372, 1579, 1684, 1870, 2064
DriI	GACNNNNNGTC	1 cut(s)	313
EaeI	YGGCCR	1 cut(s)	1641
Eam1104I	CTCTTC	5 cut(s)	17, 38, 574, 1020, 1194
Eam1105I	GACNNNNNGTC	1 cut(s)	313
EarI	CTCTTC	5 cut(s)	17, 38, 574, 1020, 1194
EciI	GGCGGA	2 cut(s)	152, 435
Ecl136II	GAGCTC	1 cut(s)	541
Eco130I	CCWWGG	1 cut(s)	1163
Eco24I	GRGCYC	4 cut(s)	286, 543, 883, 1032
Eco31I	GGTCTC	3 cut(s)	52, 404, 848
Eco32I	GATATC	1 cut(s)	1435
Eco47I	GGWCC	4 cut(s)	610, 733, 781, 941
Eco53kI	GAGCTC	1 cut(s)	541
Eco57I	CTGAAG	1 cut(s)	1815
Eco81I	CCTNAGG	1 cut(s)	795
Eco88I	CYCGRG	2 cut(s)	542, 746
EcoICRI	GAGCTC	1 cut(s)	541
EcoO109I	RGGNCCY	1 cut(s)	733
EcoRII	CCWGG	8 cut(s)	291, 783, 937, 966, 1303, 1638, 1899, 1957
EcoRV	GATATC	1 cut(s)	1435
EcoT14I	CCWWGG	1 cut(s)	1163
EcoT38I	GRGCYC	4 cut(s)	286, 543, 883, 1032
ErhI	CCWWGG	1 cut(s)	1163
Esp3I	CGTCTC	2 cut(s)	73, 2109
FaeI	CATG	5 cut(s)	60, 832, 1487, 1601, 1970
FalI	AAGNNNNNCTT	2 cut(s)	479, 511
FaqI	GGGAC	3 cut(s)	729, 746, 1635
FatI	CATG	5 cut(s)	56, 828, 1483, 1597, 1966
FauI	CCCGC	5 cut(s)	258, 395, 551, 922, 1851
FblI	GTMKAC	1 cut(s)	762
Fnu4HI	GCNGC	9 cut(s)	833, 849, 890, 911, 918, 1046, 1107, 1782, 2097
FokI	GGATG	7 cut(s)	383, 811, 1205, 1331, 1423, 1576, 1731
FriOI	GRGCYC	4 cut(s)	286, 543, 883, 1032
Fsp4HI	GCNGC	9 cut(s)	833, 849, 890, 911, 918, 1046, 1107, 1782, 2097
FspBI	CTAG	2 cut(s)	1164, 1628
GlaI	GCGC	5 cut(s)	217, 369, 480, 531, 636
GluI	GCNGC	9 cut(s)	833, 849, 890, 911, 918, 1046, 1107, 1782, 2097
GsaI	CCCAGC	2 cut(s)	121, 536
GsuI	CTGGAG	1 cut(s)	2089
HapII	CCGG	8 cut(s)	243, 280, 313, 414, 613, 747, 756, 1992
HhaI	GCGC	5 cut(s)	218, 370, 481, 532, 637
Hin1II	CATG	5 cut(s)	60, 832, 1487, 1601, 1970
Hin6I	GCGC	5 cut(s)	216, 368, 479, 530, 635
HinP1I	GCGC	5 cut(s)	216, 368, 479, 530, 635
HincII	GTYRAC	4 cut(s)	8, 405, 763, 1087
HindII	GTYRAC	4 cut(s)	8, 405, 763, 1087
HpaI	GTTAAC	1 cut(s)	405
HpaII	CCGG	8 cut(s)	243, 280, 313, 414, 613, 747, 756, 1992
HphI	GGTGA	8 cut(s)	42, 214, 559, 580, 683, 964, 997, 1973
Hpy166II	GTNNAC	8 cut(s)	8, 394, 405, 550, 654, 763, 1071, 1087
Hpy188I	TCNGA	7 cut(s)	816, 875, 1024, 1462, 1593, 1909, 2126
Hpy188III	TCNNGA	5 cut(s)	597, 613, 1376, 1766, 2068
Hpy8I	GTNNAC	8 cut(s)	8, 394, 405, 550, 654, 763, 1071, 1087
Hpy99I	CGWCG	4 cut(s)	86, 437, 767, 1649
HpyAV	CCTTC	9 cut(s)	73, 505, 610, 688, 869, 1506, 1913, 1993, 2033
HpyCH4III	ACNGT	5 cut(s)	437, 632, 949, 1396, 1929
HpyCH4IV	ACGT	4 cut(s)	552, 591, 1073, 2118
HpyF10VI	GCNNNNNNNGC	9 cut(s)	191, 487, 491, 538, 643, 848, 1550, 1781, 1790
HpyF3I	CTNAG	9 cut(s)	299, 486, 740, 795, 1224, 1329, 1445, 1814, 2108
HpySE526I	ACGT	4 cut(s)	552, 591, 1073, 2118
Hsp92II	CATG	5 cut(s)	60, 832, 1487, 1601, 1970
HspAI	GCGC	5 cut(s)	216, 368, 479, 530, 635
KflI	GGGWCCC	1 cut(s)	733
Kpn2I	TCCGGA	1 cut(s)	612
KroI	GCCGGC	1 cut(s)	242
KroNI	GCCGGC	1 cut(s)	244
KspAI	GTTAAC	1 cut(s)	405
KspI	CCGCGG	2 cut(s)	843, 918
Kzo9I	GATC	7 cut(s)	473, 1217, 1372, 1579, 1684, 1870, 2064
LguI	GCTCTTC	1 cut(s)	1020
LmnI	GCTCC	4 cut(s)	389, 538, 767, 886
Lsp1109I	GCAGC	4 cut(s)	897, 1032, 1118, 2083
LweI	GCATC	8 cut(s)	664, 1054, 1134, 1183, 1540, 1869, 1948, 1993
MaeI	CTAG	2 cut(s)	1164, 1628
MaeII	ACGT	4 cut(s)	552, 591, 1073, 2118
MaeIII	GTNAC	1 cut(s)	1354
MalI	GATC	7 cut(s)	475, 1219, 1374, 1581, 1686, 1872, 2066
MboI	GATC	7 cut(s)	473, 1217, 1372, 1579, 1684, 1870, 2064
MflI	RGATCY	4 cut(s)	1217, 1372, 1579, 2064
MhlI	GDGCHC	5 cut(s)	286, 543, 883, 1032, 1864
MluCI	AATT	8 cut(s)	139, 819, 955, 1518, 1653, 1769, 2052, 2101
MlyI	GAGTC	4 cut(s)	297, 751, 1373, 1437
MmeI	TCCRAC	1 cut(s)	1136
MroI	TCCGGA	1 cut(s)	612
MroNI	GCCGGC	1 cut(s)	242
MseI	TTAA	5 cut(s)	404, 420, 690, 1368, 1691
MslI	CAYNNNNRTG	4 cut(s)	55, 435, 1062, 1505
MspA1I	CMGCKG	5 cut(s)	558, 835, 842, 917, 1333
MspI	CCGG	8 cut(s)	243, 280, 313, 414, 613, 747, 756, 1992
Mva1269I	GAATGC	1 cut(s)	109
MvaI	CCWGG	8 cut(s)	293, 785, 939, 968, 1305, 1640, 1901, 1959
MvnI	CGCG	5 cut(s)	216, 368, 390, 842, 917
MwoI	GCNNNNNNNGC	9 cut(s)	191, 487, 491, 538, 643, 848, 1550, 1781, 1790
NaeI	GCCGGC	1 cut(s)	244
NciI	CCSGG	6 cut(s)	281, 313, 747, 748, 757, 1992
NdeII	GATC	7 cut(s)	473, 1217, 1372, 1579, 1684, 1870, 2064
NgoMIV	GCCGGC	1 cut(s)	242
NlaIII	CATG	5 cut(s)	60, 832, 1487, 1601, 1970
NlaIV	GGNNCC	9 cut(s)	247, 284, 385, 734, 735, 769, 882, 1258, 1390
NmeAIII	GCCGAG	3 cut(s)	646, 796, 877
NspI	RCATGY	3 cut(s)	60, 1487, 1601
OliI	CACNNNNGTG	1 cut(s)	435
PciI	ACATGT	1 cut(s)	1597
PciSI	GCTCTTC	1 cut(s)	1020
PcsI	WCGNNNNNNNCGW	5 cut(s)	387, 438, 513, 549, 768
PctI	GAATGC	1 cut(s)	109
PdiI	GCCGGC	1 cut(s)	244
PfeI	GAWTC	7 cut(s)	905, 991, 1159, 1457, 1492, 1649, 1904
PflFI	GACNNNGTC	2 cut(s)	437, 1382
PflMI	CCANNNNNTGG	1 cut(s)	292
PfoI	TCCNGGA	2 cut(s)	1899, 1990
PkrI	GCNGC	9 cut(s)	834, 850, 891, 912, 919, 1047, 1108, 1783, 2098
PleI	GAGTC	4 cut(s)	297, 750, 1373, 1437
PpsI	GAGTC	4 cut(s)	297, 750, 1373, 1437
PpuMI	RGGWCCY	1 cut(s)	733
PscI	ACATGT	1 cut(s)	1597
PsiI	TTATAA	1 cut(s)	1701
Psp124BI	GAGCTC	1 cut(s)	543
Psp5II	RGGWCCY	1 cut(s)	733
Psp6I	CCWGG	8 cut(s)	291, 783, 937, 966, 1303, 1638, 1899, 1957
PspFI	CCCAGC	2 cut(s)	117, 532
PspGI	CCWGG	8 cut(s)	291, 783, 937, 966, 1303, 1638, 1899, 1957
PspN4I	GGNNCC	9 cut(s)	247, 284, 385, 734, 735, 769, 882, 1258, 1390
PspOMI	GGGCCC	1 cut(s)	282
PspPPI	RGGWCCY	1 cut(s)	733
PstI	CTGCAG	1 cut(s)	1108
PstNI	CAGNNNCTG	1 cut(s)	1813
PsuI	RGATCY	4 cut(s)	1217, 1372, 1579, 2064
PsyI	GACNNNGTC	2 cut(s)	437, 1382
PvuII	CAGCTG	1 cut(s)	1333
RsaI	GTAC	3 cut(s)	452, 653, 1511
RsaNI	GTAC	3 cut(s)	451, 652, 1510
RseI	CAYNNNNRTG	4 cut(s)	55, 435, 1062, 1505
SacI	GAGCTC	1 cut(s)	543
SacII	CCGCGG	2 cut(s)	843, 918
SalI	GTCGAC	1 cut(s)	761
SapI	GCTCTTC	1 cut(s)	1020
SaqAI	TTAA	5 cut(s)	404, 420, 690, 1368, 1691
SatI	GCNGC	9 cut(s)	833, 849, 890, 911, 918, 1046, 1107, 1782, 2097
Sau3AI	GATC	7 cut(s)	473, 1217, 1372, 1579, 1684, 1870, 2064
SchI	GAGTC	4 cut(s)	297, 751, 1373, 1437
SduI	GDGCHC	5 cut(s)	286, 543, 883, 1032, 1864
SfaNI	GCATC	8 cut(s)	664, 1054, 1134, 1183, 1540, 1869, 1948, 1993
SfcI	CTRYAG	2 cut(s)	1104, 1183
Sfr303I	CCGCGG	2 cut(s)	843, 918
SgrBI	CCGCGG	2 cut(s)	843, 918
SinI	GGWCC	4 cut(s)	610, 733, 781, 941
SmaI	CCCGGG	1 cut(s)	748
SmiMI	CAYNNNNRTG	4 cut(s)	55, 435, 1062, 1505
SmlI	CTYRAG	2 cut(s)	1349, 1934
SmoI	CTYRAG	2 cut(s)	1349, 1934
Sse9I	AATT	8 cut(s)	139, 819, 955, 1518, 1653, 1769, 2052, 2101
SspMI	CTAG	2 cut(s)	1164, 1628
SstI	GAGCTC	1 cut(s)	543
StyI	CCWWGG	1 cut(s)	1163
TaaI	ACNGT	5 cut(s)	437, 632, 949, 1396, 1929
TaiI	ACGT	4 cut(s)	555, 594, 1076, 2121
TaqI	TCGA	5 cut(s)	261, 306, 762, 1318, 2014
TaqII	GACCGA	1 cut(s)	958
TasI	AATT	8 cut(s)	139, 819, 955, 1518, 1653, 1769, 2052, 2101
TatI	WGTACW	2 cut(s)	651, 1509
TauI	GCSGC	5 cut(s)	835, 851, 892, 920, 1784
TfiI	GAWTC	7 cut(s)	905, 991, 1159, 1457, 1492, 1649, 1904
Tru1I	TTAA	5 cut(s)	404, 420, 690, 1368, 1691
Tru9I	TTAA	5 cut(s)	404, 420, 690, 1368, 1691
TscAI	CASTG	9 cut(s)	475, 744, 927, 954, 1399, 1453, 1546, 1869, 1934
TseI	GCWGC	4 cut(s)	910, 1045, 1106, 2096
TspDTI	ATGAA	3 cut(s)	734, 1416, 1815
TspGWI	ACGGA	3 cut(s)	527, 780, 1517
TspMI	CCCGGG	1 cut(s)	746
TspRI	CASTG	9 cut(s)	475, 744, 927, 954, 1399, 1453, 1546, 1869, 1934
Tth111I	GACNNNGTC	2 cut(s)	437, 1382
Van91I	CCANNNNNTGG	1 cut(s)	292
VpaK11BI	GGWCC	4 cut(s)	610, 733, 781, 941
XapI	RAATTY	6 cut(s)	139, 819, 955, 1769, 2052, 2101
XceI	RCATGY	3 cut(s)	60, 1487, 1601
XcmI	CCANNNNNNNNNTGG	1 cut(s)	604
XmaI	CCCGGG	1 cut(s)	746
XmaJI	CCTAGG	1 cut(s)	1163
XmiI	GTMKAC	1 cut(s)	762
XspI	CTAG	2 cut(s)	1164, 1628

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.