Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0012144

Physical Location & Seq

Genomic Coordinates Forward (+)

4395311 .. 4400102

Gene Structure 4792 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1046000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2127 bp

ATGGGTCAGCTCCTACCTAGCTCCACCACCCTCTTCACTTCAAAGCCCTCACCCACGTGCACCTTCCTCTCCCTCCTCATCATCGGCATTCTGGGTTTTTATACCCTGCATTTCCCCCACCCTCTACTCTCCTCCTCAAATTCCCATTCTGCCCTCCGATTCCGCCAGATTTTCCTCTCCCACGCCACCAACGCCACCCTCTCCTCCTACCTCCGCGCCCTCACCCTTCACCCCCACCTCGCCGGGACCCCGCCTTCTGTCGACACTTTCAACTTCGTCCGGGCCCACTTCCTGGGCCTGGGCCTCGACACCCGCTCGGCCCACTACAACGCCCTCCTCTCCTACCCACTCCACTCCTCTCTCTCTGCACATTTTGGCAACGGCTCCCGCGTGGACATCCCCTTAACCGAGCCCGGCCTCAACAATAATCGCGACGGCGTCGTTCCGCCGTACCACGCGTACTCCCCCTCCGGGTCGGCGCACGCTAAGCTCGCCTTCGCCAACCACGGCACAGACGAGGACTATCGTGCGCTGGGGGAGCTCGGGGTGAACGTTAGCGGGTGCGTGGTGATTGTTAGAAGAGGTGATGACGTGTCCAGAGGCGAAGTGGTTAGGAAGGCCGAGAAAAACGGTGCGCTGGCTGTACTATTGTACACCGAGGGGGCCGGTGGTGATGGGAATGGGGGGTTTAAGAAGGGTTTTGAGAGAGGGTTTGTTATGAACGGCGTGGGGGACCCACTGAGCCCTGGGTGGGCCGGGGTCGACGGAGCCGAGAGGCTGGACCTGGACGACCCTGAGGTACTGAAAAGGTTTCCAAAAATTCCATCCACGCCCCTGTCCGCGGAGGCTGCCGAATCCCTTCTGGGGTGGCTCGGCGGCGCTACTGTGCCGGCAGCGTGGCGGGAATCGCTGCCCGCCGCAGTGCGCGGTGTGGGGCCTGGCCCGACAGTGGTGAATTTCACTTACAAGGGGGAGAAAAAGGTGGTGAAGATTCATAATGTTTTTGCTGTCATAAGGGGTTCAGAAGAGCCCGACCGCTATGTGCTGCTTGGAAACCATAGAGATGCGTGGACATATGGGGCTGTTGACCCCAATAGTGGAACTGCAGCATTACTTGACATTGCTCGTAGATATTCTCTTTTGATGCGTTCAGGTTGGAATCCTAGGAGGACGATTATTCTCTGTAGTTGGGACGCAGAAGAGTTTGGAATGGTAGGATCTACTGAGTGGGTTGAACAAAACATTGCCAATCTGGGCTCCAAAGCCGTTGCCTACCTTAATGTAGATTGTGCAGTTCAAGGGCCAGGATTCTTTGTCCGCTCGACTCCTCAGCTGGACAATCTGATTCTTGAGGTCACAAAGAAGGTTAAAGATCCAGACTCGGTGGGTGCCACTGTATATGAGAAATGGATGACAGCAAATAGAAGGGTTGATGTCCAAAGACTAAGTGCAGTGGATTCTGATTTTGCCCCATTTTTGCAACATGCAGGGATTCCTTCCATGGATATGTATTATGGAATTGATTTTTCTGTTTATCACACTGCATACGACGCTTATGAGTGGATGATAAATTTTGGAGATCCTTTCTTTCAGAGACATGTGGCTGTTGCTGGAATGTGGGGACTTCTTGCCCTTCACCTGGCTGACGATTTAGTTCTACCTTTCAATTTCCTCTCTTATGCGGATCAGTTAAAGCATTATAAAGATGTTTTGAGCAACTTGTTGGACGGGGGTGTATCTCTACATCCCCTCACAACAGCAATTCAGGAGTTTGCCTATGCCGCCAAAGAAGCTGAAGATGAAGCACAGAAGCTGAGAGACCAAGAAAATACAAGTGAGCTTGTAATTTTGAAGACGCGGGCACTGAATGATCGGCTGATGTTTGCTGAAAGAGGCTTCCTGGATTCAGATGGAATCGAAGGAAGACAGTGGTTCAAGCATCTTGTTTATGGGCCTCCTGGTGAGCATGGAAGCAAACTATCCTTCTTTCCCGGAGTAGGCGATGCAATGCTTCGAGCAAAGAGAATGAGTAGGACAGAAGGGGAGGCCAAAGTTCAGCACGAGATCTGGAGAGTTGCCCGGGCCATACAGAGGGCTGAAAAATCCCTGAGAGGAGACGTTTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0004180 GO:0004181 GO:0005575 GO:0006508 GO:0006807 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0008233 GO:0008235 GO:0008237 GO:0008238 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009640 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009908 GO:0009909 GO:0010015 GO:0010051 GO:0010073 GO:0010074 GO:0010075 GO:0010080 GO:0010081 GO:0010082 GO:0010154 GO:0010305 GO:0016020 GO:0016021 GO:0016787 GO:0019538 GO:0019827 GO:0022414 GO:0022622 GO:0031224 GO:0032501 GO:0032502 GO:0040008 GO:0043170 GO:0044238 GO:0044425 GO:0048316 GO:0048364 GO:0048367 GO:0048507 GO:0048509 GO:0048580 GO:0048608 GO:0048638 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050896 GO:0051239 GO:0061458 GO:0065007 GO:0070011 GO:0071704 GO:0080050 GO:0090567 GO:0098727 GO:0099402 GO:0140096 GO:1901564 GO:1905392 GO:2000026 GO:2000034 GO:2000241 GO:2000280

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PA Peptidase_M28 TFR_dimer

Protein Analysis

709

Amino Acids

77.6

Weight (kDa)

6.32

Isoelectric Point (pI)

29.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PA	PF02225	166 - 226	3.8e-06	PA domain
Peptidase_M28	PF04389	333 - 520	2.8e-24	Peptidase family M28
TFR_dimer	PF04253	579 - 703	1e-29	Transferrin receptor-like dimerisation domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012144)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G54720
fragaria_vesca	FvH4_6g21160 FvH4_6g21160 FvH4_6g21160
malus_domestica	MD12G1047100.v1.1 MD14G1046000.v1.1
prunus_persica	Prupe.7G088100_v2.0.a1
pyrus_communis	pycom12g04070 pycom14g03860
rosa_chinensis	RchiOBHm_Chr3g0477621
rosa_laevigata	RLG00000023705
rosa_multiflora	Rmu_sc0000775.1_g000003
rosa_roxburghii	Rroxscaffold_6G00403800
rosa_rugosa	Rorug03G0162500
rosa_samantha	Rh3AG212400 Rh3BG246100 Rh3CG239700 Rh3DG239200
rosa_wichuraiana	Rw3G019230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1701
AccB1I	GGYRCC	1 cut(s)	1388
AccB7I	CCANNNNNTGG	1 cut(s)	292
AccBSI	CCGCTC	2 cut(s)	315, 1320
AccI	GTMKAC	2 cut(s)	261, 762
AccII	CGCG	7 cut(s)	216, 390, 432, 458, 842, 927, 1858
AclI	AACGTT	1 cut(s)	552
AclWI	GGATC	4 cut(s)	1225, 1367, 1574, 1692
AcsI	RAATTY	4 cut(s)	139, 819, 955, 1570
AcuI	CTGAAG	1 cut(s)	1815
AcvI	CACGTG	1 cut(s)	57
AcyI	GRCGYC	1 cut(s)	438
AfaI	GTAC	5 cut(s)	452, 461, 645, 653, 801
AflIII	ACRYGT	3 cut(s)	456, 591, 1597
AgsI	TTSAA	7 cut(s)	42, 271, 1235, 1298, 1666, 1852, 1936
AjiI	CACGTC	1 cut(s)	592
AjnI	CCWGG	9 cut(s)	291, 297, 745, 783, 937, 1303, 1638, 1899, 1957
AleI	CACNNNNGTG	1 cut(s)	55
AluBI	AGCT	8 cut(s)	10, 21, 490, 541, 1333, 1793, 1813, 1840
AluI	AGCT	8 cut(s)	10, 21, 490, 541, 1333, 1793, 1813, 1840
Alw21I	GWGCWC	2 cut(s)	62, 543
Alw26I	GTCTC	3 cut(s)	1588, 1812, 2109
Alw44I	GTGCAC	1 cut(s)	58
AlwI	GGATC	4 cut(s)	1225, 1367, 1574, 1692
AlwNI	CAGNNNCTG	1 cut(s)	1813
Ama87I	CYCGRG	2 cut(s)	542, 2079
ApaI	GGGCCC	1 cut(s)	286
ApaLI	GTGCAC	1 cut(s)	58
ApeKI	GCWGC	5 cut(s)	848, 893, 910, 1045, 1106
ApoI	RAATTY	4 cut(s)	139, 819, 955, 1570
Asp700I	GAANNNNTTC	1 cut(s)	858
AspA2I	CCTAGG	1 cut(s)	1163
AspLEI	GCGC	6 cut(s)	218, 481, 532, 637, 881, 927
AsuC2I	CCSGG	8 cut(s)	244, 281, 414, 472, 757, 1992, 2080, 2081
AvaI	CYCGRG	2 cut(s)	542, 2079
AvaII	GGWCC	3 cut(s)	246, 733, 781
AvrII	CCTAGG	1 cut(s)	1163
AxyI	CCTNAGG	1 cut(s)	795
BaeGI	GKGCMC	3 cut(s)	62, 286, 1864
BanI	GGYRCC	1 cut(s)	1388
BanII	GRGCYC	6 cut(s)	286, 414, 543, 746, 1032, 1259
BauI	CACGAG	1 cut(s)	2060
BbrPI	CACGTG	1 cut(s)	57
BbsI	GAAGAC	2 cut(s)	1859, 1930
Bbv12I	GWGCWC	2 cut(s)	62, 543
BbvCI	CCTCAGC	1 cut(s)	1329
BbvI	GCAGC	5 cut(s)	835, 897, 905, 1032, 1118
BccI	CCATC	3 cut(s)	668, 832, 1904
BceAI	ACGGC	6 cut(s)	397, 433, 451, 523, 739, 1250
BciT130I	CCWGG	9 cut(s)	293, 299, 747, 785, 939, 1305, 1640, 1901, 1959
BcnI	CCSGG	8 cut(s)	244, 281, 414, 472, 757, 1992, 2080, 2081
BcoDI	GTCTC	3 cut(s)	1588, 1812, 2109
BfaI	CTAG	2 cut(s)	18, 1164
BfmI	CTRYAG	2 cut(s)	1104, 1183
BfoI	RGCGCY	1 cut(s)	882
BglII	AGATCT	1 cut(s)	2064
BisI	GCNGC	8 cut(s)	849, 877, 894, 911, 918, 1046, 1107, 1782
BlnI	CCTAGG	1 cut(s)	1163
BlpI	GCTNAGC	1 cut(s)	486
BlsI	GCNGC	8 cut(s)	850, 878, 895, 912, 919, 1047, 1108, 1783
Bme18I	GGWCC	3 cut(s)	246, 733, 781
BmeT110I	CYCGRG	2 cut(s)	542, 2079
BmgBI	CACGTC	1 cut(s)	592
BmsI	GCATC	4 cut(s)	1054, 1134, 1948, 1993
BpiI	GAAGAC	2 cut(s)	1859, 1930
BpmI	CTGGAG	1 cut(s)	2089
Bpu10I	CCTNAGC	1 cut(s)	1329
Bpu1102I	GCTNAGC	1 cut(s)	486
BpuEI	CTTGAG	1 cut(s)	1370
BpuMI	CCSGG	8 cut(s)	244, 281, 414, 472, 757, 1992, 2080, 2081
BsaAI	YACGTR	1 cut(s)	57
BsaHI	GRCGYC	1 cut(s)	438
BsaI	GGTCTC	1 cut(s)	1812
BsaXI	ACNNNNNCTCC	4 cut(s)	446, 452, 476, 482
Bse118I	RCCGGY	2 cut(s)	665, 889
Bse21I	CCTNAGG	1 cut(s)	795
Bse3DI	GCAATG	3 cut(s)	1119, 1242, 2013
BseBI	CCWGG	9 cut(s)	293, 299, 747, 785, 939, 1305, 1640, 1901, 1959
BseGI	GGATG	5 cut(s)	396, 824, 1416, 1569, 1744
BseMI	GCAATG	3 cut(s)	1119, 1242, 2013
BseMII	CTCAG	6 cut(s)	731, 786, 1215, 1343, 1805, 2099
BseRI	GAGGAG	8 cut(s)	65, 121, 124, 193, 326, 346, 1317, 2127
BseSI	GKGCMC	3 cut(s)	62, 286, 1864
BseXI	GCAGC	5 cut(s)	835, 897, 905, 1032, 1118
BseYI	CCCAGC	1 cut(s)	532
BsgI	GTGCAG	3 cut(s)	351, 1311, 1470
Bsh1236I	CGCG	7 cut(s)	216, 390, 432, 458, 842, 927, 1858
Bsh1285I	CGRYCG	1 cut(s)	1036
BshNI	GGYRCC	1 cut(s)	1388
BsiEI	CGRYCG	1 cut(s)	1036
BsiHKAI	GWGCWC	2 cut(s)	62, 543
BsiHKCI	CYCGRG	2 cut(s)	542, 2079
BsiSI	CCGG	9 cut(s)	243, 280, 414, 471, 666, 756, 890, 1992, 2080
BslFI	GGGAC	4 cut(s)	259, 746, 1205, 1635
BsmAI	GTCTC	3 cut(s)	1588, 1812, 2109
BsmBI	CGTCTC	1 cut(s)	2109
BsmFI	GGGAC	4 cut(s)	259, 746, 1205, 1635
BsmI	GAATGC	1 cut(s)	87
Bso31I	GGTCTC	1 cut(s)	1812
BsoBI	CYCGRG	2 cut(s)	542, 2079
Bsp120I	GGGCCC	1 cut(s)	282
Bsp1286I	GDGCHC	8 cut(s)	62, 286, 414, 543, 746, 1032, 1259, 1864
Bsp1407I	TGTACA	1 cut(s)	651
Bsp143I	GATC	6 cut(s)	1217, 1372, 1579, 1684, 1870, 2064
Bsp1720I	GCTNAGC	1 cut(s)	486
Bsp19I	CCATGG	1 cut(s)	1500
Bsp68I	TCGCGA	1 cut(s)	432
BspCNI	CTCAG	6 cut(s)	732, 787, 1216, 1342, 1806, 2100
BspFNI	CGCG	7 cut(s)	216, 390, 432, 458, 842, 927, 1858
BspMAI	CTGCAG	1 cut(s)	1108
BspPI	GGATC	4 cut(s)	1225, 1367, 1574, 1692
BspQI	GCTCTTC	1 cut(s)	1020
BspT107I	GGYRCC	1 cut(s)	1388
BspTNI	GGTCTC	1 cut(s)	1812
BsrBI	CCGCTC	2 cut(s)	315, 1320
BsrDI	GCAATG	3 cut(s)	1119, 1242, 2013
BsrFI	RCCGGY	2 cut(s)	665, 889
BsrGI	TGTACA	1 cut(s)	651
BssAI	RCCGGY	2 cut(s)	665, 889
BssMI	GATC	6 cut(s)	1217, 1372, 1579, 1684, 1870, 2064
BssNI	GRCGYC	1 cut(s)	438
BssSI	CACGAG	1 cut(s)	2060
BssT1I	CCWWGG	2 cut(s)	1163, 1500
Bst2BI	CACGAG	1 cut(s)	2060
Bst2UI	CCWGG	9 cut(s)	293, 299, 747, 785, 939, 1305, 1640, 1901, 1959
Bst4CI	ACNGT	5 cut(s)	632, 886, 949, 1396, 1929
Bst6I	CTCTTC	4 cut(s)	38, 574, 1020, 1194
BstACI	GRCGYC	1 cut(s)	438
BstAUI	TGTACA	1 cut(s)	651
BstBAI	YACGTR	1 cut(s)	57
BstC8I	GCNNGC	6 cut(s)	483, 492, 639, 891, 915, 1860
BstDEI	CTNAG	8 cut(s)	486, 740, 795, 1224, 1329, 1445, 1814, 2108
BstDSI	CCRYGG	3 cut(s)	505, 840, 1500
BstF5I	GGATG	5 cut(s)	396, 824, 1416, 1569, 1744
BstFNI	CGCG	7 cut(s)	216, 390, 432, 458, 842, 927, 1858
BstH2I	RGCGCY	1 cut(s)	882
BstHHI	GCGC	6 cut(s)	218, 481, 532, 637, 881, 927
BstKTI	GATC	6 cut(s)	1220, 1375, 1582, 1687, 1873, 2067
BstMAI	GTCTC	3 cut(s)	1588, 1812, 2109
BstMBI	GATC	6 cut(s)	1217, 1372, 1579, 1684, 1870, 2064
BstMCI	CGRYCG	1 cut(s)	1036
BstMWI	GCNNNNNNNGC	9 cut(s)	191, 487, 491, 538, 848, 907, 1550, 1781, 1790
BstNI	CCWGG	9 cut(s)	293, 299, 747, 785, 939, 1305, 1640, 1901, 1959
BstNSI	RCATGY	2 cut(s)	1487, 1601
BstSFI	CTRYAG	2 cut(s)	1104, 1183
BstSLI	GKGCMC	3 cut(s)	62, 286, 1864
BstUI	CGCG	7 cut(s)	216, 390, 432, 458, 842, 927, 1858
BstV1I	GCAGC	5 cut(s)	835, 897, 905, 1032, 1118
BstV2I	GAAGAC	2 cut(s)	1859, 1930
BstX2I	RGATCY	4 cut(s)	1217, 1372, 1579, 2064
BstYI	RGATCY	4 cut(s)	1217, 1372, 1579, 2064
Bsu36I	CCTNAGG	1 cut(s)	795
BtgI	CCRYGG	3 cut(s)	505, 840, 1500
BtgZI	GCGATG	1 cut(s)	2016
BtrI	CACGTC	1 cut(s)	592
BtsCI	GGATG	5 cut(s)	396, 824, 1416, 1569, 1744
BtsI	GCAGTG	3 cut(s)	927, 1458, 1539
BtsIMutI	CAGTG	8 cut(s)	737, 927, 954, 1392, 1458, 1539, 1862, 1934
BtuMI	TCGCGA	1 cut(s)	432
Cac8I	GCNNGC	6 cut(s)	483, 492, 639, 891, 915, 1860
CaiI	CAGNNNCTG	1 cut(s)	1813
CfoI	GCGC	6 cut(s)	218, 481, 532, 637, 881, 927
Cfr10I	RCCGGY	2 cut(s)	665, 889
Cfr42I	CCGCGG	1 cut(s)	843
Cfr9I	CCCGGG	1 cut(s)	2079
CseI	GACGC	4 cut(s)	427, 1202, 1559, 1864
Csp6I	GTAC	5 cut(s)	451, 460, 644, 652, 800
CviAII	CATG	4 cut(s)	1484, 1501, 1598, 1967
CviQI	GTAC	5 cut(s)	451, 460, 644, 652, 800
DdeI	CTNAG	8 cut(s)	486, 740, 795, 1224, 1329, 1445, 1814, 2108
DpnI	GATC	6 cut(s)	1219, 1374, 1581, 1686, 1872, 2066
DpnII	GATC	6 cut(s)	1217, 1372, 1579, 1684, 1870, 2064
Eam1104I	CTCTTC	4 cut(s)	38, 574, 1020, 1194
EarI	CTCTTC	4 cut(s)	38, 574, 1020, 1194
EciI	GGCGGA	2 cut(s)	152, 435
Ecl136II	GAGCTC	1 cut(s)	541
Eco130I	CCWWGG	2 cut(s)	1163, 1500
Eco24I	GRGCYC	6 cut(s)	286, 414, 543, 746, 1032, 1259
Eco31I	GGTCTC	1 cut(s)	1812
Eco47I	GGWCC	3 cut(s)	246, 733, 781
Eco53kI	GAGCTC	1 cut(s)	541
Eco57I	CTGAAG	1 cut(s)	1815
Eco72I	CACGTG	1 cut(s)	57
Eco81I	CCTNAGG	1 cut(s)	795
Eco88I	CYCGRG	2 cut(s)	542, 2079
EcoICRI	GAGCTC	1 cut(s)	541
EcoO109I	RGGNCCY	3 cut(s)	246, 733, 935
EcoRII	CCWGG	9 cut(s)	291, 297, 745, 783, 937, 1303, 1638, 1899, 1957
EcoT14I	CCWWGG	2 cut(s)	1163, 1500
EcoT38I	GRGCYC	6 cut(s)	286, 414, 543, 746, 1032, 1259
ErhI	CCWWGG	2 cut(s)	1163, 1500
Esp3I	CGTCTC	1 cut(s)	2109
FaeI	CATG	4 cut(s)	1487, 1504, 1601, 1970
FalI	AAGNNNNNCTT	2 cut(s)	479, 511
FaqI	GGGAC	4 cut(s)	259, 746, 1205, 1635
FatI	CATG	4 cut(s)	1483, 1500, 1597, 1966
FauI	CCCGC	7 cut(s)	258, 320, 395, 551, 894, 922, 1851
FauNDI	CATATG	1 cut(s)	1075
FblI	GTMKAC	2 cut(s)	261, 762
Fnu4HI	GCNGC	8 cut(s)	849, 877, 894, 911, 918, 1046, 1107, 1782
FokI	GGATG	5 cut(s)	383, 811, 1423, 1576, 1731
FriOI	GRGCYC	6 cut(s)	286, 414, 543, 746, 1032, 1259
Fsp4HI	GCNGC	8 cut(s)	849, 877, 894, 911, 918, 1046, 1107, 1782
FspBI	CTAG	2 cut(s)	18, 1164
GlaI	GCGC	6 cut(s)	217, 480, 531, 636, 880, 926
GluI	GCNGC	8 cut(s)	849, 877, 894, 911, 918, 1046, 1107, 1782
GsaI	CCCAGC	1 cut(s)	536
GsuI	CTGGAG	1 cut(s)	2089
HaeII	RGCGCY	1 cut(s)	882
HapII	CCGG	9 cut(s)	243, 280, 414, 471, 666, 756, 890, 1992, 2080
HgaI	GACGC	4 cut(s)	427, 1202, 1559, 1864
HhaI	GCGC	6 cut(s)	218, 481, 532, 637, 881, 927
Hin1I	GRCGYC	1 cut(s)	438
Hin1II	CATG	4 cut(s)	1487, 1504, 1601, 1970
Hin6I	GCGC	6 cut(s)	216, 479, 530, 635, 879, 925
HinP1I	GCGC	6 cut(s)	216, 479, 530, 635, 879, 925
HincII	GTYRAC	3 cut(s)	262, 763, 1087
HindII	GTYRAC	3 cut(s)	262, 763, 1087
HpaII	CCGG	9 cut(s)	243, 280, 414, 471, 666, 756, 890, 1992, 2080
Hpy166II	GTNNAC	8 cut(s)	60, 262, 394, 550, 654, 763, 1071, 1087
Hpy188I	TCNGA	7 cut(s)	158, 1024, 1344, 1462, 1593, 1909, 2126
Hpy188III	TCNNGA	6 cut(s)	431, 597, 1349, 1376, 1766, 2068
Hpy8I	GTNNAC	8 cut(s)	60, 262, 394, 550, 654, 763, 1071, 1087
Hpy99I	CGWCG	4 cut(s)	437, 443, 767, 1553
HpyCH4III	ACNGT	5 cut(s)	632, 886, 949, 1396, 1929
HpyCH4IV	ACGT	4 cut(s)	56, 552, 591, 2118
HpyF10VI	GCNNNNNNNGC	9 cut(s)	191, 487, 491, 538, 848, 907, 1550, 1781, 1790
HpyF3I	CTNAG	8 cut(s)	486, 740, 795, 1224, 1329, 1445, 1814, 2108
HpySE526I	ACGT	4 cut(s)	56, 552, 591, 2118
Hsp92I	GRCGYC	1 cut(s)	438
Hsp92II	CATG	4 cut(s)	1487, 1504, 1601, 1970
HspAI	GCGC	6 cut(s)	216, 479, 530, 635, 879, 925
KflI	GGGWCCC	2 cut(s)	246, 733
KroI	GCCGGC	1 cut(s)	889
KroNI	GCCGGC	1 cut(s)	891
KspI	CCGCGG	1 cut(s)	843
Kzo9I	GATC	6 cut(s)	1217, 1372, 1579, 1684, 1870, 2064
LguI	GCTCTTC	1 cut(s)	1020
LmnI	GCTCC	6 cut(s)	15, 26, 389, 538, 767, 1262
Lsp1109I	GCAGC	5 cut(s)	835, 897, 905, 1032, 1118
LweI	GCATC	4 cut(s)	1054, 1134, 1948, 1993
MaeI	CTAG	2 cut(s)	18, 1164
MaeII	ACGT	4 cut(s)	56, 552, 591, 2118
MaeIII	GTNAC	1 cut(s)	1354
MalI	GATC	6 cut(s)	1219, 1374, 1581, 1686, 1872, 2066
MbiI	CCGCTC	2 cut(s)	315, 1320
MboI	GATC	6 cut(s)	1217, 1372, 1579, 1684, 1870, 2064
MboII	GAAGA	8 cut(s)	25, 591, 1000, 1037, 1211, 1808, 1864, 1935
MflI	RGATCY	4 cut(s)	1217, 1372, 1579, 2064
MhlI	GDGCHC	8 cut(s)	62, 286, 414, 543, 746, 1032, 1259, 1864
MluCI	AATT	8 cut(s)	139, 819, 955, 1518, 1570, 1666, 1761, 1845
MluI	ACGCGT	1 cut(s)	456
MlyI	GAGTC	2 cut(s)	1318, 1373
MmeI	TCCRAC	2 cut(s)	1136, 1704
MroNI	GCCGGC	1 cut(s)	889
MroXI	GAANNNNTTC	1 cut(s)	858
MseI	TTAA	5 cut(s)	404, 690, 1278, 1368, 1691
MslI	CAYNNNNRTG	3 cut(s)	55, 1062, 1505
MspA1I	CMGCKG	2 cut(s)	842, 1333
MspI	CCGG	9 cut(s)	243, 280, 414, 471, 666, 756, 890, 1992, 2080
Mva1269I	GAATGC	1 cut(s)	87
MvaI	CCWGG	9 cut(s)	293, 299, 747, 785, 939, 1305, 1640, 1901, 1959
MvnI	CGCG	7 cut(s)	216, 390, 432, 458, 842, 927, 1858
MwoI	GCNNNNNNNGC	9 cut(s)	191, 487, 491, 538, 848, 907, 1550, 1781, 1790
NaeI	GCCGGC	1 cut(s)	891
NciI	CCSGG	8 cut(s)	244, 281, 414, 472, 757, 1992, 2080, 2081
NcoI	CCATGG	1 cut(s)	1500
NdeI	CATATG	1 cut(s)	1075
NdeII	GATC	6 cut(s)	1217, 1372, 1579, 1684, 1870, 2064
NgoMIV	GCCGGC	1 cut(s)	889
NlaIII	CATG	4 cut(s)	1487, 1504, 1601, 1970
NmeAIII	GCCGAG	4 cut(s)	296, 646, 796, 852
NmuCI	GTSAC	1 cut(s)	1354
NruI	TCGCGA	1 cut(s)	432
NspI	RCATGY	2 cut(s)	1487, 1601
OliI	CACNNNNGTG	1 cut(s)	55
PasI	CCCWGGG	1 cut(s)	746
PciI	ACATGT	1 cut(s)	1597
PciSI	GCTCTTC	1 cut(s)	1020
PcsI	WCGNNNNNNNCGW	4 cut(s)	387, 513, 549, 768
PctI	GAATGC	1 cut(s)	87
PdiI	GCCGGC	1 cut(s)	891
PdmI	GAANNNNTTC	1 cut(s)	858
PflFI	GACNNNGTC	1 cut(s)	437
PflMI	CCANNNNNTGG	1 cut(s)	292
PfoI	TCCNGGA	2 cut(s)	1899, 1990
PkrI	GCNGC	8 cut(s)	850, 878, 895, 912, 919, 1047, 1108, 1783
PleI	GAGTC	2 cut(s)	1318, 1373
PmaCI	CACGTG	1 cut(s)	57
PmlI	CACGTG	1 cut(s)	57
PpsI	GAGTC	2 cut(s)	1318, 1373
Ppu21I	YACGTR	1 cut(s)	57
PpuMI	RGGWCCY	2 cut(s)	246, 733
PscI	ACATGT	1 cut(s)	1597
PsiI	TTATAA	1 cut(s)	1701
Psp124BI	GAGCTC	1 cut(s)	543
Psp1406I	AACGTT	1 cut(s)	552
Psp5II	RGGWCCY	2 cut(s)	246, 733
Psp6I	CCWGG	9 cut(s)	291, 297, 745, 783, 937, 1303, 1638, 1899, 1957
PspCI	CACGTG	1 cut(s)	57
PspFI	CCCAGC	1 cut(s)	532
PspGI	CCWGG	9 cut(s)	291, 297, 745, 783, 937, 1303, 1638, 1899, 1957
PspOMI	GGGCCC	1 cut(s)	282
PspPPI	RGGWCCY	2 cut(s)	246, 733
PstI	CTGCAG	1 cut(s)	1108
PstNI	CAGNNNCTG	1 cut(s)	1813
PsuI	RGATCY	4 cut(s)	1217, 1372, 1579, 2064
PsyI	GACNNNGTC	1 cut(s)	437
PvuII	CAGCTG	1 cut(s)	1333
RruI	TCGCGA	1 cut(s)	432
RsaI	GTAC	5 cut(s)	452, 461, 645, 653, 801
RsaNI	GTAC	5 cut(s)	451, 460, 644, 652, 800
RseI	CAYNNNNRTG	3 cut(s)	55, 1062, 1505
SacI	GAGCTC	1 cut(s)	543
SacII	CCGCGG	1 cut(s)	843
SalI	GTCGAC	2 cut(s)	260, 761
SapI	GCTCTTC	1 cut(s)	1020
SaqAI	TTAA	5 cut(s)	404, 690, 1278, 1368, 1691
SatI	GCNGC	8 cut(s)	849, 877, 894, 911, 918, 1046, 1107, 1782
Sau3AI	GATC	6 cut(s)	1217, 1372, 1579, 1684, 1870, 2064
SchI	GAGTC	2 cut(s)	1318, 1373
SduI	GDGCHC	8 cut(s)	62, 286, 414, 543, 746, 1032, 1259, 1864
SfaNI	GCATC	4 cut(s)	1054, 1134, 1948, 1993
SfcI	CTRYAG	2 cut(s)	1104, 1183
Sfr303I	CCGCGG	1 cut(s)	843
SgrBI	CCGCGG	1 cut(s)	843
SinI	GGWCC	3 cut(s)	246, 733, 781
SmaI	CCCGGG	1 cut(s)	2081
SmiMI	CAYNNNNRTG	3 cut(s)	55, 1062, 1505
SmlI	CTYRAG	1 cut(s)	1349
SmoI	CTYRAG	1 cut(s)	1349
SrfI	GCCCGGGC	1 cut(s)	2081
Sse9I	AATT	8 cut(s)	139, 819, 955, 1518, 1570, 1666, 1761, 1845
SspMI	CTAG	2 cut(s)	18, 1164
SstI	GAGCTC	1 cut(s)	543
StyI	CCWWGG	2 cut(s)	1163, 1500
TaaI	ACNGT	5 cut(s)	632, 886, 949, 1396, 1929
TaiI	ACGT	4 cut(s)	59, 555, 594, 2121
TaqI	TCGA	6 cut(s)	261, 306, 762, 1322, 1917, 2014
TasI	AATT	8 cut(s)	139, 819, 955, 1518, 1570, 1666, 1761, 1845
TatI	WGTACW	2 cut(s)	643, 651
TauI	GCSGC	3 cut(s)	879, 920, 1784
Tru1I	TTAA	5 cut(s)	404, 690, 1278, 1368, 1691
Tru9I	TTAA	5 cut(s)	404, 690, 1278, 1368, 1691
TscAI	CASTG	8 cut(s)	744, 927, 954, 1399, 1458, 1546, 1869, 1934
TseFI	GTSAC	1 cut(s)	1354
TseI	GCWGC	5 cut(s)	848, 893, 910, 1045, 1106
Tsp45I	GTSAC	1 cut(s)	1354
TspDTI	ATGAA	3 cut(s)	734, 983, 1815
TspGWI	ACGGA	1 cut(s)	780
TspMI	CCCGGG	1 cut(s)	2079
TspRI	CASTG	8 cut(s)	744, 927, 954, 1399, 1458, 1546, 1869, 1934
Tth111I	GACNNNGTC	1 cut(s)	437
Van91I	CCANNNNNTGG	1 cut(s)	292
VneI	GTGCAC	1 cut(s)	58
VpaK11BI	GGWCC	3 cut(s)	246, 733, 781
XapI	RAATTY	4 cut(s)	139, 819, 955, 1570
XceI	RCATGY	2 cut(s)	1487, 1601
XcmI	CCANNNNNNNNNTGG	1 cut(s)	604
XmaI	CCCGGG	1 cut(s)	2079
XmaJI	CCTAGG	1 cut(s)	1163
XmiI	GTMKAC	2 cut(s)	261, 762
XmnI	GAANNNNTTC	1 cut(s)	858
XspI	CTAG	2 cut(s)	18, 1164

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.