AT3G55440

triosephosphate isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0007425
Physical Location & Seq
Forward (+)
20553403 .. 20556560
3158 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G55440.1

Sequence Viewer

Length: 765 bp
ATGGCCAGAAAGTTCTTCGTCGGAGGCAACTGGAAATGTAACGGAACTGCTGAGGAGGTGAAGAAGATTGTGAACACTCTTAATGAAGCTCAGGTTCCTTCACAGGATGTTGTAGAGGTTGTGGTTAGCCCTCCATATGTTTTTCTTCCCCTGGTTAAGAGCACATTGAGGTCTGACTTTTTTGTTGCGGCACAAAACTGTTGGGTTAAGAAAGGAGGTGCTTTCACTGGTGAAGTGAGTGCGGAGATGCTTGTGAACTTGGATATTCCATGGGTTATCCTTGGTCACTCTGAAAGGAGGGCAATCCTTAATGAATCAAGCGAGTTCGTCGGAGACAAGGTTGCCTATGCACTTGCTCAAGGTTTGAAAGTGATTGCTTGTGTTGGTGAGACTCTTGAGGAGCGGGAAGCTGGATCGACCATGGATGTTGTGGCTGCCCAGACCAAGGCTATTGCCGATCGGGTGACAAACTGGTCAAATGTTGTCATAGCCTATGAACCAGTGTGGGCCATTGGAACCGGAAAGGTCGCTAGCCCAGCCCAAGCTCAAGAAGTACACGATGAGCTGAGGAAATGGCTGGCCAAGAACGTGAGTGCTGATGTCGCTGCTACAACCCGCATCATTTATGGAGGATCCGTCAATGGTGGTAACTGCAAGGAGCTAGGTGGACAGGCCGATGTTGATGGTTTCTTGGTCGGTGGTGCTTCTCTAAAGCCTGAGTTTATCGACATCATTAAGGCCGCAGAGGTGAAGAAAAGTGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004807 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0005911 GO:0005975 GO:0005996 GO:0006006 GO:0006066 GO:0006071 GO:0006081 GO:0006082 GO:0006090 GO:0006091 GO:0006094 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009506 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010043 GO:0016020 GO:0016051 GO:0016052 GO:0016053 GO:0016310 GO:0016853 GO:0016860 GO:0016861 GO:0017144 GO:0018130 GO:0019318 GO:0019319 GO:0019359 GO:0019362 GO:0019363 GO:0019400 GO:0019405 GO:0019438 GO:0019439 GO:0019563 GO:0019637 GO:0019682 GO:0019693 GO:0019751 GO:0019752 GO:0030054 GO:0030312 GO:0031090 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042221 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044275 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046164 GO:0046166 GO:0046174 GO:0046184 GO:0046364 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046872 GO:0046914 GO:0046939 GO:0048046 GO:0050896 GO:0051186 GO:0051188 GO:0055044 GO:0055086 GO:0071704 GO:0071944 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:0098588 GO:0098805 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1901615 GO:1901616
KEGG Pathways
Metabolic & Signaling
ko00010 ko00051 ko00562 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00051 map00562 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
TIM

Protein Analysis

254

Amino Acids

27.17

Weight (kDa)

5.39

Isoelectric Point (pI)

24.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIM PF00121 6 - 248 1.2e-90 Triosephosphate isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 472
AccBSI CCGCTC 1 cut(s) 403
AciI CCGC 5 cut(s) 188, 242, 403, 616, 741
AclWI GGATC 3 cut(s) 421, 627, 640
AcoI YGGCCR 2 cut(s) 3, 579
AfaI GTAC 1 cut(s) 555
AfiI CCNNNNNNNGG 1 cut(s) 445
AgsI TTSAA 1 cut(s) 367
AjnI CCWGG 1 cut(s) 150
AluBI AGCT 5 cut(s) 89, 410, 545, 565, 661
AluI AGCT 5 cut(s) 89, 410, 545, 565, 661
Alw21I GWGCWC 1 cut(s) 164
Alw26I GTCTC 2 cut(s) 327, 383
AlwI GGATC 3 cut(s) 421, 627, 640
AoxI GGCC 5 cut(s) 3, 507, 579, 672, 738
ApeKI GCWGC 2 cut(s) 434, 605
AspS9I GGNCC 1 cut(s) 507
AsuHPI GGTGA 5 cut(s) 70, 242, 398, 475, 760
AsuNHI GCTAGC 1 cut(s) 530
BalI TGGCCA 2 cut(s) 5, 581
BamHI GGATCC 1 cut(s) 632
Bbv12I GWGCWC 1 cut(s) 164
BbvCI CCTCAGC 2 cut(s) 51, 566
BbvI GCAGC 2 cut(s) 421, 592
BccI CCATC 1 cut(s) 677
BciT130I CCWGG 1 cut(s) 152
BcoDI GTCTC 2 cut(s) 327, 383
BfaI CTAG 2 cut(s) 531, 662
BisI GCNGC 4 cut(s) 189, 435, 606, 741
BlsI GCNGC 4 cut(s) 190, 436, 607, 742
Bme1390I CCNGG 1 cut(s) 152
BmgT120I GGNCC 1 cut(s) 507
BmiI GGNNCC 3 cut(s) 96, 517, 634
BmrFI CCNGG 1 cut(s) 152
BmsI GCATC 2 cut(s) 237, 627
BmtI GCTAGC 1 cut(s) 534
Bpu10I CCTNAGC 3 cut(s) 51, 90, 566
BpuEI CTTGAG 3 cut(s) 342, 416, 531
BsaJI CCNNGG 5 cut(s) 150, 269, 280, 420, 444
BsaWI WCCGGW 1 cut(s) 518
BsaXI ACNNNNNCTCC 4 cut(s) 324, 354, 621, 651
Bsc4I CCNNNNNNNGG 1 cut(s) 445
Bse1I ACTGG 4 cut(s) 35, 232, 476, 500
BseBI CCWGG 1 cut(s) 152
BseDI CCNNGG 5 cut(s) 150, 269, 280, 420, 444
BseGI GGATG 2 cut(s) 112, 430
BseLI CCNNNNNNNGG 1 cut(s) 445
BseMII CTCAG 4 cut(s) 42, 104, 557, 708
BseNI ACTGG 4 cut(s) 35, 232, 476, 500
BseRI GAGGAG 2 cut(s) 68, 413
BseXI GCAGC 2 cut(s) 421, 592
BseYI CCCAGC 1 cut(s) 535
Bsh1285I CGRYCG 1 cut(s) 460
BshFI GGCC 5 cut(s) 5, 509, 581, 674, 740
BsiEI CGRYCG 1 cut(s) 460
BsiHKAI GWGCWC 1 cut(s) 164
BsiSI CCGG 1 cut(s) 519
BslI CCNNNNNNNGG 1 cut(s) 445
BsmAI GTCTC 2 cut(s) 327, 383
BsnI GGCC 5 cut(s) 5, 509, 581, 674, 740
Bsp1286I GDGCHC 1 cut(s) 164
Bsp143I GATC 3 cut(s) 413, 457, 632
Bsp19I CCATGG 2 cut(s) 269, 420
BspACI CCGC 5 cut(s) 188, 242, 403, 616, 741
BspANI GGCC 5 cut(s) 5, 509, 581, 674, 740
BspCNI CTCAG 4 cut(s) 43, 103, 558, 709
BspLI GGNNCC 3 cut(s) 96, 517, 634
BspOI GCTAGC 1 cut(s) 534
BspPI GGATC 3 cut(s) 421, 627, 640
BsrBI CCGCTC 1 cut(s) 403
BsrI ACTGG 4 cut(s) 35, 232, 476, 500
BssECI CCNNGG 5 cut(s) 150, 269, 280, 420, 444
BssMI GATC 3 cut(s) 413, 457, 632
BssT1I CCWWGG 4 cut(s) 269, 280, 420, 444
Bst2UI CCWGG 1 cut(s) 152
Bst4CI ACNGT 1 cut(s) 200
BstC8I GCNNGC 2 cut(s) 532, 579
BstDEI CTNAG 4 cut(s) 51, 90, 566, 717
BstDSI CCRYGG 2 cut(s) 269, 420
BstF5I GGATG 2 cut(s) 112, 430
BstKTI GATC 3 cut(s) 416, 460, 635
BstMAI GTCTC 2 cut(s) 327, 383
BstMBI GATC 3 cut(s) 413, 457, 632
BstMCI CGRYCG 1 cut(s) 460
BstMWI GCNNNNNNNGC 2 cut(s) 536, 602
BstNI CCWGG 1 cut(s) 152
BstSCI CCNGG 1 cut(s) 150
BstV1I GCAGC 2 cut(s) 421, 592
BstX2I RGATCY 1 cut(s) 632
BstYI RGATCY 1 cut(s) 632
BsuRI GGCC 5 cut(s) 5, 509, 581, 674, 740
BtgI CCRYGG 2 cut(s) 269, 420
BtsCI GGATG 2 cut(s) 112, 430
BtsIMutI CAGTG 2 cut(s) 225, 507
Cac8I GCNNGC 2 cut(s) 532, 579
Cfr13I GGNCC 1 cut(s) 507
Csp6I GTAC 1 cut(s) 554
CviAII CATG 2 cut(s) 270, 421
CviQI GTAC 1 cut(s) 554
DdeI CTNAG 4 cut(s) 51, 90, 566, 717
DpnI GATC 3 cut(s) 415, 459, 634
DpnII GATC 3 cut(s) 413, 457, 632
DrdI GACNNNNNNGTC 1 cut(s) 472
DseDI GACNNNNNNGTC 1 cut(s) 472
EaeI YGGCCR 2 cut(s) 3, 579
Eco130I CCWWGG 4 cut(s) 269, 280, 420, 444
EcoRII CCWGG 1 cut(s) 150
EcoT14I CCWWGG 4 cut(s) 269, 280, 420, 444
ErhI CCWWGG 4 cut(s) 269, 280, 420, 444
FaeI CATG 2 cut(s) 273, 424
FaiI YATR 8 cut(s) 136, 138, 271, 348, 422, 488, 495, 627
FatI CATG 2 cut(s) 269, 420
FauI CCCGC 2 cut(s) 396, 623
FauNDI CATATG 1 cut(s) 136
Fnu4HI GCNGC 4 cut(s) 189, 435, 606, 741
FokI GGATG 2 cut(s) 119, 437
Fsp4HI GCNGC 4 cut(s) 189, 435, 606, 741
FspBI CTAG 2 cut(s) 531, 662
GluI GCNGC 4 cut(s) 189, 435, 606, 741
GsaI CCCAGC 1 cut(s) 539
HaeIII GGCC 5 cut(s) 5, 509, 581, 674, 740
HapII CCGG 1 cut(s) 519
Hin1II CATG 2 cut(s) 273, 424
HinfI GANTC 2 cut(s) 314, 391
HpaII CCGG 1 cut(s) 519
HphI GGTGA 5 cut(s) 70, 242, 398, 475, 760
Hpy166II GTNNAC 4 cut(s) 73, 256, 556, 668
Hpy188I TCNGA 4 cut(s) 23, 175, 292, 332
Hpy188III TCNNGA 2 cut(s) 395, 548
Hpy8I GTNNAC 4 cut(s) 73, 256, 556, 668
Hpy99I CGWCG 2 cut(s) 23, 332
HpyAV CCTTC 1 cut(s) 108
HpyCH4III ACNGT 1 cut(s) 200
HpyCH4IV ACGT 1 cut(s) 588
HpyCH4V TGCA 2 cut(s) 350, 654
HpyF10VI GCNNNNNNNGC 2 cut(s) 536, 602
HpyF3I CTNAG 4 cut(s) 51, 90, 566, 717
HpySE526I ACGT 1 cut(s) 588
Hsp92II CATG 2 cut(s) 273, 424
Kzo9I GATC 3 cut(s) 413, 457, 632
LmnI GCTCC 2 cut(s) 400, 658
Lsp1109I GCAGC 2 cut(s) 421, 592
LweI GCATC 2 cut(s) 237, 627
MaeI CTAG 2 cut(s) 531, 662
MaeII ACGT 1 cut(s) 588
MaeIII GTNAC 4 cut(s) 38, 284, 463, 647
MalI GATC 3 cut(s) 415, 459, 634
MbiI CCGCTC 1 cut(s) 403
MboI GATC 3 cut(s) 413, 457, 632
MboII GAAGA 5 cut(s) 7, 73, 76, 137, 763
MflI RGATCY 1 cut(s) 632
MhlI GDGCHC 1 cut(s) 164
MlsI TGGCCA 2 cut(s) 5, 581
MluNI TGGCCA 2 cut(s) 5, 581
MlyI GAGTC 1 cut(s) 385
MmeI TCCRAC 1 cut(s) 310
Mox20I TGGCCA 2 cut(s) 5, 581
MscI TGGCCA 2 cut(s) 5, 581
MseI TTAA 5 cut(s) 81, 156, 207, 309, 735
Msp20I TGGCCA 2 cut(s) 5, 581
MspI CCGG 1 cut(s) 519
MspR9I CCNGG 1 cut(s) 152
MvaI CCWGG 1 cut(s) 152
MwoI GCNNNNNNNGC 2 cut(s) 536, 602
NcoI CCATGG 2 cut(s) 269, 420
NdeI CATATG 1 cut(s) 136
NdeII GATC 3 cut(s) 413, 457, 632
NheI GCTAGC 1 cut(s) 530
NlaIII CATG 2 cut(s) 273, 424
NlaIV GGNNCC 3 cut(s) 96, 517, 634
NmuCI GTSAC 2 cut(s) 284, 463
PfeI GAWTC 1 cut(s) 314
PkrI GCNGC 4 cut(s) 190, 436, 607, 742
Ple19I CGATCG 1 cut(s) 460
PleI GAGTC 1 cut(s) 385
PpsI GAGTC 1 cut(s) 385
Psp6I CCWGG 1 cut(s) 150
PspFI CCCAGC 1 cut(s) 535
PspGI CCWGG 1 cut(s) 150
PspN4I GGNNCC 3 cut(s) 96, 517, 634
PspPI GGNCC 1 cut(s) 507
PsuI RGATCY 1 cut(s) 632
PvuI CGATCG 1 cut(s) 460
RsaI GTAC 1 cut(s) 555
RsaNI GTAC 1 cut(s) 554
SaqAI TTAA 5 cut(s) 81, 156, 207, 309, 735
SatI GCNGC 4 cut(s) 189, 435, 606, 741
Sau3AI GATC 3 cut(s) 413, 457, 632
Sau96I GGNCC 1 cut(s) 507
SchI GAGTC 1 cut(s) 385
ScrFI CCNGG 1 cut(s) 152
SduI GDGCHC 1 cut(s) 164
SfaNI GCATC 2 cut(s) 237, 627
SmlI CTYRAG 3 cut(s) 357, 395, 546
SmoI CTYRAG 3 cut(s) 357, 395, 546
SsiI CCGC 5 cut(s) 188, 242, 403, 616, 741
SspMI CTAG 2 cut(s) 531, 662
StyD4I CCNGG 1 cut(s) 150
StyI CCWWGG 4 cut(s) 269, 280, 420, 444
TaaI ACNGT 1 cut(s) 200
TaiI ACGT 1 cut(s) 591
TaqI TCGA 2 cut(s) 416, 726
TatI WGTACW 1 cut(s) 553
TauI GCSGC 2 cut(s) 191, 743
TfiI GAWTC 1 cut(s) 314
Tru1I TTAA 5 cut(s) 81, 156, 207, 309, 735
Tru9I TTAA 5 cut(s) 81, 156, 207, 309, 735
TscAI CASTG 2 cut(s) 232, 507
TseFI GTSAC 2 cut(s) 284, 463
TseI GCWGC 2 cut(s) 434, 605
Tsp45I GTSAC 2 cut(s) 284, 463
TspDTI ATGAA 3 cut(s) 99, 327, 510
TspGWI ACGGA 2 cut(s) 57, 625
TspRI CASTG 2 cut(s) 232, 507
XcmI CCANNNNNNNNNTGG 1 cut(s) 427
XspI CTAG 2 cut(s) 531, 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.