Rmu_sc0011000.1_g000007

triosephosphate isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0011000.1
OG0007425
Physical Location & Seq
Forward (+)
22343 .. 25103
2761 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0011000.1_g000007.1.cds

Sequence Viewer

Length: 762 bp
atgggcaggaagtttttcgtcggcggcaactggaaatgcaatggaacaatcgatgaggttaagaagatcgtgaacttgctcaatgagggaaaagtgccgtcacaagatgttgtggaggttgtggttagcccaccgtatgtgtttcttccattggtgaagagcactttgaggccagatttccatgttgcggcacagaactgctgggtcaagaagggtggtgccttcactggtgaagttagtgctgagatgcttaaaaatctagaggttccatgggtcatccttggtcattccgaaagaagagctcttttgggtgaatccaatgagtttgttggggataaggttgcttatgcactttcccaaggtttgaaggtgatcgcctgtgttggtgagacccttgagcagcgagaagctggatcgactatggaagtagttgctgcacagacaaaggcaattgcagagaaagtgaaagattggaccaacattgttttggcctatgagccagtgtgggctattggaactgggaaggttgcttctccagctcaggctcaggaagtgcattgtgaattgaggaaatggcttcatgcaaacacaagtccggaggttgctgcaacaaccaggattatatatggaggctctgtcaatggtgcaaactccaaagagctggcaggccagcccgatcttgatgggtttttggttggcggagcttccttaaagcccgagtttattgacattatcaacgctgctgaggtgaagaaagcctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00051 ko00562 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00051 map00562 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
TIM

Protein Analysis

253

Amino Acids

27.24

Weight (kDa)

6.14

Isoelectric Point (pI)

24.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 218
AccIII TCCGGA 1 cut(s) 595
AciI CCGC 3 cut(s) 24, 188, 699
AclWI GGATC 1 cut(s) 421
AfiI CCNNNNNNNGG 1 cut(s) 187
AgsI TTSAA 1 cut(s) 367
AjnI CCWGG 1 cut(s) 614
AluBI AGCT 5 cut(s) 302, 410, 539, 661, 704
AluI AGCT 5 cut(s) 302, 410, 539, 661, 704
Alw21I GWGCWC 2 cut(s) 164, 304
Alw26I GTCTC 1 cut(s) 383
AlwI GGATC 1 cut(s) 421
Ama87I CYCGRG 1 cut(s) 716
Aor13HI TCCGGA 1 cut(s) 595
AoxI GGCC 3 cut(s) 170, 489, 667
ApeKI GCWGC 4 cut(s) 400, 434, 605, 740
Asp700I GAANNNNTTC 1 cut(s) 14
AspS9I GGNCC 1 cut(s) 474
AsuHPI GGTGA 6 cut(s) 166, 242, 323, 382, 398, 760
AvaI CYCGRG 1 cut(s) 716
AvaII GGWCC 1 cut(s) 474
BanI GGYRCC 1 cut(s) 218
BanII GRGCYC 1 cut(s) 304
Bbv12I GWGCWC 2 cut(s) 164, 304
BbvCI CCTCAGC 1 cut(s) 744
BbvI GCAGC 4 cut(s) 412, 421, 592, 727
BccI CCATC 1 cut(s) 677
BceAI ACGGC 1 cut(s) 82
BciT130I CCWGG 1 cut(s) 616
BcoDI GTCTC 1 cut(s) 383
BfaI CTAG 2 cut(s) 260, 760
BisI GCNGC 6 cut(s) 25, 189, 401, 435, 606, 741
BlsI GCNGC 6 cut(s) 26, 190, 402, 436, 607, 742
Bme1390I CCNGG 1 cut(s) 616
Bme18I GGWCC 1 cut(s) 474
BmeT110I CYCGRG 1 cut(s) 716
BmgT120I GGNCC 1 cut(s) 474
BmiI GGNNCC 2 cut(s) 220, 267
BmrFI CCNGG 1 cut(s) 616
BmrI ACTGGG 1 cut(s) 528
BmsI GCATC 1 cut(s) 237
BmuI ACTGGG 1 cut(s) 528
BpmI CTGGAG 1 cut(s) 519
Bpu10I CCTNAGC 3 cut(s) 540, 546, 744
BpuEI CTTGAG 1 cut(s) 416
Bsa29I ATCGAT 1 cut(s) 51
BsaI GGTCTC 1 cut(s) 383
BsaJI CCNNGG 3 cut(s) 269, 280, 358
BsaWI WCCGGW 1 cut(s) 595
BsaXI ACNNNNNCTCC 2 cut(s) 621, 651
Bsc4I CCNNNNNNNGG 1 cut(s) 187
Bse1I ACTGG 4 cut(s) 35, 232, 500, 523
Bse3DI GCAATG 1 cut(s) 46
BseAI TCCGGA 1 cut(s) 595
BseBI CCWGG 1 cut(s) 616
BseCI ATCGAT 1 cut(s) 51
BseDI CCNNGG 3 cut(s) 269, 280, 358
BseGI GGATG 1 cut(s) 276
BseLI CCNNNNNNNGG 1 cut(s) 187
BseMI GCAATG 1 cut(s) 46
BseMII CTCAG 4 cut(s) 234, 554, 560, 735
BseNI ACTGG 4 cut(s) 35, 232, 500, 523
BseXI GCAGC 4 cut(s) 412, 421, 592, 727
BseYI CCCAGC 1 cut(s) 201
BsgI GTGCAG 1 cut(s) 420
BshFI GGCC 3 cut(s) 172, 491, 669
BshNI GGYRCC 1 cut(s) 218
BshVI ATCGAT 1 cut(s) 51
BsiHKAI GWGCWC 2 cut(s) 164, 304
BsiHKCI CYCGRG 1 cut(s) 716
BsiSI CCGG 1 cut(s) 596
BslI CCNNNNNNNGG 1 cut(s) 187
BsmAI GTCTC 1 cut(s) 383
BsnI GGCC 3 cut(s) 172, 491, 669
Bso31I GGTCTC 1 cut(s) 383
BsoBI CYCGRG 1 cut(s) 716
Bsp1286I GDGCHC 2 cut(s) 164, 304
Bsp13I TCCGGA 1 cut(s) 595
Bsp143I GATC 4 cut(s) 66, 372, 413, 676
Bsp19I CCATGG 1 cut(s) 269
BspACI CCGC 3 cut(s) 24, 188, 699
BspANI GGCC 3 cut(s) 172, 491, 669
BspCNI CTCAG 4 cut(s) 235, 553, 559, 736
BspDI ATCGAT 1 cut(s) 51
BspEI TCCGGA 1 cut(s) 595
BspLI GGNNCC 2 cut(s) 220, 267
BspPI GGATC 1 cut(s) 421
BspQI GCTCTTC 2 cut(s) 152, 292
BspT107I GGYRCC 1 cut(s) 218
BspTNI GGTCTC 1 cut(s) 383
BsrDI GCAATG 1 cut(s) 46
BsrI ACTGG 4 cut(s) 35, 232, 500, 523
BssECI CCNNGG 3 cut(s) 269, 280, 358
BssMI GATC 4 cut(s) 66, 372, 413, 676
BssT1I CCWWGG 3 cut(s) 269, 280, 358
Bst2UI CCWGG 1 cut(s) 616
Bst4CI ACNGT 1 cut(s) 135
Bst6I CTCTTC 2 cut(s) 152, 292
BstC8I GCNNGC 3 cut(s) 663, 667, 671
BstDEI CTNAG 4 cut(s) 243, 540, 546, 744
BstDSI CCRYGG 1 cut(s) 269
BstF5I GGATG 1 cut(s) 276
BstKTI GATC 4 cut(s) 69, 375, 416, 679
BstMAI GTCTC 1 cut(s) 383
BstMBI GATC 4 cut(s) 66, 372, 413, 676
BstMWI GCNNNNNNNGC 1 cut(s) 536
BstNI CCWGG 1 cut(s) 616
BstSCI CCNGG 1 cut(s) 614
BstV1I GCAGC 4 cut(s) 412, 421, 592, 727
BstXI CCANNNNNNTGG 1 cut(s) 661
Bsu15I ATCGAT 1 cut(s) 51
BsuRI GGCC 3 cut(s) 172, 491, 669
BsuTUI ATCGAT 1 cut(s) 51
BtgI CCRYGG 1 cut(s) 269
BtsCI GGATG 1 cut(s) 276
BtsIMutI CAGTG 2 cut(s) 225, 507
Cac8I GCNNGC 3 cut(s) 663, 667, 671
Cfr13I GGNCC 1 cut(s) 474
ClaI ATCGAT 1 cut(s) 51
CspCI CAANNNNNGTGG 2 cut(s) 196, 231
CviAII CATG 3 cut(s) 182, 270, 581
DdeI CTNAG 4 cut(s) 243, 540, 546, 744
DpnI GATC 4 cut(s) 68, 374, 415, 678
DpnII GATC 4 cut(s) 66, 372, 413, 676
Eam1104I CTCTTC 2 cut(s) 152, 292
EarI CTCTTC 2 cut(s) 152, 292
EciI GGCGGA 1 cut(s) 714
Ecl136II GAGCTC 1 cut(s) 302
Eco130I CCWWGG 3 cut(s) 269, 280, 358
Eco24I GRGCYC 1 cut(s) 304
Eco31I GGTCTC 1 cut(s) 383
Eco47I GGWCC 1 cut(s) 474
Eco53kI GAGCTC 1 cut(s) 302
Eco88I CYCGRG 1 cut(s) 716
EcoICRI GAGCTC 1 cut(s) 302
EcoRII CCWGG 1 cut(s) 614
EcoT14I CCWWGG 3 cut(s) 269, 280, 358
EcoT38I GRGCYC 1 cut(s) 304
ErhI CCWWGG 3 cut(s) 269, 280, 358
FaeI CATG 3 cut(s) 185, 273, 584
FatI CATG 3 cut(s) 181, 269, 580
Fnu4HI GCNGC 6 cut(s) 25, 189, 401, 435, 606, 741
FokI GGATG 1 cut(s) 263
FriOI GRGCYC 1 cut(s) 304
Fsp4HI GCNGC 6 cut(s) 25, 189, 401, 435, 606, 741
FspBI CTAG 2 cut(s) 260, 760
GluI GCNGC 6 cut(s) 25, 189, 401, 435, 606, 741
GsaI CCCAGC 1 cut(s) 205
GsuI CTGGAG 1 cut(s) 519
HaeIII GGCC 3 cut(s) 172, 491, 669
HapII CCGG 1 cut(s) 596
Hin1II CATG 3 cut(s) 185, 273, 584
HinfI GANTC 1 cut(s) 314
HpaII CCGG 1 cut(s) 596
HphI GGTGA 6 cut(s) 166, 242, 323, 382, 398, 760
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 1 cut(s) 292
Hpy188III TCNNGA 6 cut(s) 70, 208, 260, 548, 596, 680
Hpy8I GTNNAC 1 cut(s) 73
Hpy99I CGWCG 1 cut(s) 23
HpyAV CCTTC 4 cut(s) 205, 232, 361, 517
HpyCH4III ACNGT 1 cut(s) 135
HpyCH4V TGCA 8 cut(s) 39, 350, 437, 455, 556, 584, 608, 647
HpyF10VI GCNNNNNNNGC 1 cut(s) 536
HpyF3I CTNAG 4 cut(s) 243, 540, 546, 744
Hsp92II CATG 3 cut(s) 185, 273, 584
Kpn2I TCCGGA 1 cut(s) 595
Kzo9I GATC 4 cut(s) 66, 372, 413, 676
LguI GCTCTTC 2 cut(s) 152, 292
LmnI GCTCC 1 cut(s) 701
Lsp1109I GCAGC 4 cut(s) 412, 421, 592, 727
LweI GCATC 1 cut(s) 237
MaeI CTAG 2 cut(s) 260, 760
MaeIII GTNAC 1 cut(s) 99
MalI GATC 4 cut(s) 68, 374, 415, 678
MboI GATC 4 cut(s) 66, 372, 413, 676
MboII GAAGA 4 cut(s) 76, 137, 169, 309
MfeI CAATTG 1 cut(s) 450
MhlI GDGCHC 2 cut(s) 164, 304
MluCI AATT 2 cut(s) 450, 563
MnlI CCTC 9 cut(s) 49, 79, 109, 162, 256, 561, 592, 623, 739
MroI TCCGGA 1 cut(s) 595
MroXI GAANNNNTTC 1 cut(s) 14
MseI TTAA 3 cut(s) 60, 252, 710
MspI CCGG 1 cut(s) 596
MspR9I CCNGG 1 cut(s) 616
MunI CAATTG 1 cut(s) 450
MvaI CCWGG 1 cut(s) 616
MwoI GCNNNNNNNGC 1 cut(s) 536
NcoI CCATGG 1 cut(s) 269
NdeII GATC 4 cut(s) 66, 372, 413, 676
NlaIII CATG 3 cut(s) 185, 273, 584
NlaIV GGNNCC 2 cut(s) 220, 267
NmuCI GTSAC 1 cut(s) 99
PciSI GCTCTTC 2 cut(s) 152, 292
PdmI GAANNNNTTC 1 cut(s) 14
PfeI GAWTC 1 cut(s) 314
PkrI GCNGC 6 cut(s) 26, 190, 402, 436, 607, 742
Psp124BI GAGCTC 1 cut(s) 304
Psp6I CCWGG 1 cut(s) 614
PspFI CCCAGC 1 cut(s) 201
PspGI CCWGG 1 cut(s) 614
PspN4I GGNNCC 2 cut(s) 220, 267
PspPI GGNCC 1 cut(s) 474
SacI GAGCTC 1 cut(s) 304
SapI GCTCTTC 2 cut(s) 152, 292
SaqAI TTAA 3 cut(s) 60, 252, 710
SatI GCNGC 6 cut(s) 25, 189, 401, 435, 606, 741
Sau3AI GATC 4 cut(s) 66, 372, 413, 676
Sau96I GGNCC 1 cut(s) 474
ScrFI CCNGG 1 cut(s) 616
SduI GDGCHC 2 cut(s) 164, 304
SfaNI GCATC 1 cut(s) 237
SinI GGWCC 1 cut(s) 474
SmlI CTYRAG 1 cut(s) 395
SmoI CTYRAG 1 cut(s) 395
Sse9I AATT 2 cut(s) 450, 563
SsiI CCGC 3 cut(s) 24, 188, 699
SspMI CTAG 2 cut(s) 260, 760
SstI GAGCTC 1 cut(s) 304
StyD4I CCNGG 1 cut(s) 614
StyI CCWWGG 3 cut(s) 269, 280, 358
TaaI ACNGT 1 cut(s) 135
TaqI TCGA 2 cut(s) 51, 416
TasI AATT 2 cut(s) 450, 563
TauI GCSGC 2 cut(s) 27, 191
TfiI GAWTC 1 cut(s) 314
Tru1I TTAA 3 cut(s) 60, 252, 710
Tru9I TTAA 3 cut(s) 60, 252, 710
TscAI CASTG 2 cut(s) 232, 507
TseFI GTSAC 1 cut(s) 99
TseI GCWGC 4 cut(s) 400, 434, 605, 740
Tsp45I GTSAC 1 cut(s) 99
TspDTI ATGAA 1 cut(s) 569
TspRI CASTG 2 cut(s) 232, 507
VpaK11BI GGWCC 1 cut(s) 474
XbaI TCTAGA 1 cut(s) 259
XcmI CCANNNNNNNNNTGG 1 cut(s) 484
XmnI GAANNNNTTC 1 cut(s) 14
XspI CTAG 2 cut(s) 260, 760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.