RchiOBHm_Chr5g0064961

Triosephosphate isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0007425
Physical Location & Seq
Forward (+)
70860849 .. 70864202
3354 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34087

Sequence Viewer

Length: 765 bp
ATGGGCAGGAAATTCTTCGTCGGTGGCAACTGGAAATGCAATGGGACAACTTCAGAGGTGAAGAAGATTGTCACCACATTGAATGAAGCTGAAGTCCCTTCAGAAGATGTTGTGGACGTTGTTGTGAGCCCTCCCTTTGTGTTTCTTGCTTATGTGAAAAGTTTGTTGCGGTCTGATTTCCATGTTGCGGCACAGAACTGTTGGGTTCGGAGAGGTGGTGCGTTGACTGGAGAGATTAGTGCTGAGATGCTGGTCAATTTGGGGATTCCCTGGGTCATTATCGGCCATTCTGAGAGAAGACAGATTCTAAATGAATCCAATGAGTTTGTTGGGGACAAAGTTGGATATGCACTTTCTCAAGGCCTGAAAGTTATTGCCTGTATTGGAGAGACTCTTGAGCAGCGAGAATCAGGATCTACAGTGGCTGTTGTTGCTGCACAAACAAAAGCAATAGCAGATAAAGTATCAAGCTGGGAGAACATTGTTTTGGCATATGAGCCAGTTTGGGCCATTGGCACTGGGAAGGTTGCAACCCCTGATCAGGCACAGCAAGTCCATTTTGAATTGAGGAAATGGCTTCAAGTCAATGTTGGTGCTGAAGTCGCTGCATCAACCAGAATTATTTACGGAGGTTCTGTAAATGGAGCAAACTGCAAAGAATTGGCAACACAGCCAGACGTTGATGGGTTTTTGGTTGGCGGAGCTTCCCTTAAGCCGGAGTTCATTGACATTATCAATTCTGCTACAGTGAAGAAAGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004807 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0005911 GO:0005975 GO:0005996 GO:0006006 GO:0006066 GO:0006071 GO:0006081 GO:0006082 GO:0006090 GO:0006091 GO:0006094 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009506 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010043 GO:0016020 GO:0016051 GO:0016052 GO:0016053 GO:0016310 GO:0016853 GO:0016860 GO:0016861 GO:0017144 GO:0018130 GO:0019318 GO:0019319 GO:0019359 GO:0019362 GO:0019363 GO:0019400 GO:0019405 GO:0019438 GO:0019439 GO:0019563 GO:0019637 GO:0019682 GO:0019693 GO:0019751 GO:0019752 GO:0030054 GO:0030312 GO:0031090 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042221 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044275 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046164 GO:0046166 GO:0046174 GO:0046184 GO:0046364 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046872 GO:0046914 GO:0046939 GO:0048046 GO:0050896 GO:0051186 GO:0051188 GO:0055044 GO:0055086 GO:0071704 GO:0071944 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:0098588 GO:0098805 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1901615 GO:1901616
KEGG Pathways
Metabolic & Signaling
ko00010 ko00051 ko00562 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00051 map00562 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
TIM

Protein Analysis

254

Amino Acids

27.36

Weight (kDa)

5.36

Isoelectric Point (pI)

25.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIM PF00121 6 - 247 4.4e-90 Triosephosphate isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 169, 188, 699
AclWI GGATC 1 cut(s) 421
AcoI YGGCCR 1 cut(s) 283
AcsI RAATTY 1 cut(s) 11
AcuI CTGAAG 4 cut(s) 36, 84, 111, 618
AfiI CCNNNNNNNGG 3 cut(s) 187, 541, 715
AflII CTTAAG 1 cut(s) 710
AgsI TTSAA 3 cut(s) 82, 563, 581
AjnI CCWGG 1 cut(s) 269
AjuI GAANNNNNNNTTGG 2 cut(s) 470, 502
AluBI AGCT 3 cut(s) 89, 471, 704
AluI AGCT 3 cut(s) 89, 471, 704
Alw26I GTCTC 1 cut(s) 383
AlwI GGATC 1 cut(s) 421
AlwNI CAGNNNCTG 1 cut(s) 425
AoxI GGCC 3 cut(s) 283, 361, 507
ApeKI GCWGC 3 cut(s) 400, 434, 605
ApoI RAATTY 1 cut(s) 11
Asp700I GAANNNNTTC 1 cut(s) 14
AspS9I GGNCC 1 cut(s) 507
AsuHPI GGTGA 2 cut(s) 64, 70
BanII GRGCYC 1 cut(s) 131
BbsI GAAGAC 1 cut(s) 304
BbvI GCAGC 3 cut(s) 412, 421, 592
BccI CCATC 1 cut(s) 677
BciT130I CCWGG 1 cut(s) 271
BclI TGATCA 1 cut(s) 538
BcoDI GTCTC 1 cut(s) 383
BfmI CTRYAG 2 cut(s) 417, 744
BfrI CTTAAG 1 cut(s) 710
BisI GCNGC 4 cut(s) 189, 401, 435, 606
BlsI GCNGC 4 cut(s) 190, 402, 436, 607
Bme1390I CCNGG 1 cut(s) 271
BmgT120I GGNCC 1 cut(s) 507
BmrFI CCNGG 1 cut(s) 271
BmrI ACTGGG 1 cut(s) 528
BmsI GCATC 2 cut(s) 237, 617
BmuI ACTGGG 1 cut(s) 528
BpiI GAAGAC 1 cut(s) 304
BpmI CTGGAG 1 cut(s) 249
BpuEI CTTGAG 2 cut(s) 342, 416
BsaJI CCNNGG 2 cut(s) 269, 270
BsaXI ACNNNNNCTCC 4 cut(s) 202, 232, 621, 651
Bsc4I CCNNNNNNNGG 3 cut(s) 187, 541, 715
Bse1I ACTGG 4 cut(s) 35, 232, 500, 523
Bse3DI GCAATG 1 cut(s) 46
BseBI CCWGG 1 cut(s) 271
BseDI CCNNGG 2 cut(s) 269, 270
BseLI CCNNNNNNNGG 3 cut(s) 187, 541, 715
BseMI GCAATG 1 cut(s) 46
BseMII CTCAG 2 cut(s) 234, 282
BseNI ACTGG 4 cut(s) 35, 232, 500, 523
BseXI GCAGC 3 cut(s) 412, 421, 592
BseYI CCCAGC 1 cut(s) 471
BsgI GTGCAG 1 cut(s) 420
BshFI GGCC 3 cut(s) 285, 363, 509
BsiSI CCGG 1 cut(s) 716
BslFI GGGAC 3 cut(s) 58, 80, 347
BslI CCNNNNNNNGG 3 cut(s) 187, 541, 715
BsmAI GTCTC 1 cut(s) 383
BsmFI GGGAC 3 cut(s) 58, 80, 347
BsnI GGCC 3 cut(s) 285, 363, 509
Bsp1286I GDGCHC 1 cut(s) 131
Bsp143I GATC 2 cut(s) 413, 538
BspACI CCGC 3 cut(s) 169, 188, 699
BspANI GGCC 3 cut(s) 285, 363, 509
BspCNI CTCAG 2 cut(s) 235, 283
BspPI GGATC 1 cut(s) 421
BspTI CTTAAG 1 cut(s) 710
BsrDI GCAATG 1 cut(s) 46
BsrI ACTGG 4 cut(s) 35, 232, 500, 523
BssECI CCNNGG 2 cut(s) 269, 270
BssMI GATC 2 cut(s) 413, 538
Bst2UI CCWGG 1 cut(s) 271
Bst4CI ACNGT 3 cut(s) 200, 421, 748
BstAFI CTTAAG 1 cut(s) 710
BstDEI CTNAG 2 cut(s) 243, 291
BstKTI GATC 2 cut(s) 416, 541
BstMAI GTCTC 1 cut(s) 383
BstMBI GATC 2 cut(s) 413, 538
BstMWI GCNNNNNNNGC 2 cut(s) 431, 602
BstNI CCWGG 1 cut(s) 271
BstSCI CCNGG 1 cut(s) 269
BstSFI CTRYAG 2 cut(s) 417, 744
BstV1I GCAGC 3 cut(s) 412, 421, 592
BstV2I GAAGAC 1 cut(s) 304
BstX2I RGATCY 1 cut(s) 413
BstYI RGATCY 1 cut(s) 413
BsuRI GGCC 3 cut(s) 285, 363, 509
BtsIMutI CAGTG 3 cut(s) 426, 516, 753
CaiI CAGNNNCTG 1 cut(s) 425
Cfr13I GGNCC 1 cut(s) 507
CviAII CATG 1 cut(s) 182
DdeI CTNAG 2 cut(s) 243, 291
DpnI GATC 2 cut(s) 415, 540
DpnII GATC 2 cut(s) 413, 538
EaeI YGGCCR 1 cut(s) 283
EciI GGCGGA 1 cut(s) 714
Eco147I AGGCCT 1 cut(s) 363
Eco24I GRGCYC 1 cut(s) 131
Eco57I CTGAAG 4 cut(s) 36, 84, 111, 618
EcoRII CCWGG 1 cut(s) 269
EcoT38I GRGCYC 1 cut(s) 131
FaeI CATG 1 cut(s) 185
FaiI YATR 5 cut(s) 153, 183, 348, 493, 495
FaqI GGGAC 3 cut(s) 58, 80, 347
FatI CATG 1 cut(s) 181
FauNDI CATATG 1 cut(s) 493
FbaI TGATCA 1 cut(s) 538
Fnu4HI GCNGC 4 cut(s) 189, 401, 435, 606
FriOI GRGCYC 1 cut(s) 131
Fsp4HI GCNGC 4 cut(s) 189, 401, 435, 606
GluI GCNGC 4 cut(s) 189, 401, 435, 606
GsaI CCCAGC 1 cut(s) 475
GsuI CTGGAG 1 cut(s) 249
HaeIII GGCC 3 cut(s) 285, 363, 509
HapII CCGG 1 cut(s) 716
Hin1II CATG 1 cut(s) 185
HincII GTYRAC 1 cut(s) 225
HindII GTYRAC 1 cut(s) 225
HinfI GANTC 5 cut(s) 265, 304, 314, 391, 407
HpaII CCGG 1 cut(s) 716
HphI GGTGA 2 cut(s) 64, 70
Hpy166II GTNNAC 2 cut(s) 115, 225
Hpy188I TCNGA 5 cut(s) 55, 103, 175, 210, 292
Hpy188III TCNNGA 2 cut(s) 395, 411
Hpy8I GTNNAC 2 cut(s) 115, 225
Hpy99I CGWCG 1 cut(s) 23
HpyAV CCTTC 2 cut(s) 108, 517
HpyCH4III ACNGT 3 cut(s) 200, 421, 748
HpyCH4IV ACGT 2 cut(s) 117, 678
HpyCH4V TGCA 6 cut(s) 39, 350, 437, 530, 608, 654
HpyF10VI GCNNNNNNNGC 2 cut(s) 431, 602
HpyF3I CTNAG 2 cut(s) 243, 291
HpySE526I ACGT 2 cut(s) 117, 678
Hsp92II CATG 1 cut(s) 185
Ksp22I TGATCA 1 cut(s) 538
Kzo9I GATC 2 cut(s) 413, 538
LmnI GCTCC 2 cut(s) 644, 701
Lsp1109I GCAGC 3 cut(s) 412, 421, 592
LweI GCATC 2 cut(s) 237, 617
MaeII ACGT 2 cut(s) 117, 678
MaeIII GTNAC 1 cut(s) 70
MalI GATC 2 cut(s) 415, 540
MboI GATC 2 cut(s) 413, 538
MboII GAAGA 6 cut(s) 7, 73, 76, 116, 309, 763
MflI RGATCY 1 cut(s) 413
MhlI GDGCHC 1 cut(s) 131
MluCI AATT 6 cut(s) 11, 256, 563, 618, 659, 736
MlyI GAGTC 1 cut(s) 385
MmeI TCCRAC 1 cut(s) 322
MnlI CCTC 5 cut(s) 49, 141, 206, 561, 623
MroXI GAANNNNTTC 1 cut(s) 14
MseI TTAA 1 cut(s) 711
MspCI CTTAAG 1 cut(s) 710
MspI CCGG 1 cut(s) 716
MspR9I CCNGG 1 cut(s) 271
MvaI CCWGG 1 cut(s) 271
MwoI GCNNNNNNNGC 2 cut(s) 431, 602
NdeI CATATG 1 cut(s) 493
NdeII GATC 2 cut(s) 413, 538
NlaIII CATG 1 cut(s) 185
NmuCI GTSAC 1 cut(s) 70
PasI CCCWGGG 1 cut(s) 270
PceI AGGCCT 1 cut(s) 363
PdmI GAANNNNTTC 1 cut(s) 14
PfeI GAWTC 4 cut(s) 265, 304, 314, 407
PkrI GCNGC 4 cut(s) 190, 402, 436, 607
PleI GAGTC 1 cut(s) 385
PpsI GAGTC 1 cut(s) 385
Psp6I CCWGG 1 cut(s) 269
PspFI CCCAGC 1 cut(s) 471
PspGI CCWGG 1 cut(s) 269
PspPI GGNCC 1 cut(s) 507
PstNI CAGNNNCTG 1 cut(s) 425
PsuI RGATCY 1 cut(s) 413
SaqAI TTAA 1 cut(s) 711
SatI GCNGC 4 cut(s) 189, 401, 435, 606
Sau3AI GATC 2 cut(s) 413, 538
Sau96I GGNCC 1 cut(s) 507
SchI GAGTC 1 cut(s) 385
ScrFI CCNGG 1 cut(s) 271
SduI GDGCHC 1 cut(s) 131
SetI ASST 9 cut(s) 60, 91, 120, 217, 473, 528, 634, 681, 706
SfaNI GCATC 2 cut(s) 237, 617
SfcI CTRYAG 2 cut(s) 417, 744
SmlI CTYRAG 3 cut(s) 357, 395, 710
SmoI CTYRAG 3 cut(s) 357, 395, 710
Sse9I AATT 6 cut(s) 11, 256, 563, 618, 659, 736
SseBI AGGCCT 1 cut(s) 363
SsiI CCGC 3 cut(s) 169, 188, 699
StuI AGGCCT 1 cut(s) 363
StyD4I CCNGG 1 cut(s) 269
TaaI ACNGT 3 cut(s) 200, 421, 748
TaiI ACGT 2 cut(s) 120, 681
TasI AATT 6 cut(s) 11, 256, 563, 618, 659, 736
TauI GCSGC 1 cut(s) 191
TfiI GAWTC 4 cut(s) 265, 304, 314, 407
Tru1I TTAA 1 cut(s) 711
Tru9I TTAA 1 cut(s) 711
TscAI CASTG 3 cut(s) 426, 523, 753
TseFI GTSAC 1 cut(s) 70
TseI GCWGC 3 cut(s) 400, 434, 605
Tsp45I GTSAC 1 cut(s) 70
TspDTI ATGAA 3 cut(s) 99, 327, 712
TspGWI ACGGA 1 cut(s) 642
TspRI CASTG 3 cut(s) 426, 523, 753
Vha464I CTTAAG 1 cut(s) 710
XapI RAATTY 1 cut(s) 11
XmnI GAANNNNTTC 1 cut(s) 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.