MD01G1134600.v1.1

triosephosphate isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0007425
Physical Location & Seq
Forward (+)
24420808 .. 24424268
3461 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1134600.v1.1.491

Sequence Viewer

Length: 765 bp
ATGGGCAGGAAGTTCTTCGTCGGCGGCAACTGGAAATGCAACGGAACCTTCGATGAGGTGAAGAAGATCGTGAACATACTCAATGAGGGACAAGTTCCATCGCAAGATGTTGTGGAGGTTGTGGTTAGCCCACCGTATGTGTTTCTTCCGGTGGTGAAGAGTTCATTGAGGCCCGAATTCCATGTCGCTGCACAGAACTGCTGGGTCAAGAAGGGTGGTGCCTTCACTGGTGAAGTTAGTGCTGAGATGCTAGTCAATCTCGAGGTTCCATGGGTCATTCTTGGTCATTCGGAAAGAAGACTGATTTTGGGTGAATCCAATGAGTTTGTTGCGGATAAGGTTGCATACGCACTTTCTCAAGGTTTGAAGGTGATAGCTTGTGTCGGTGAGACCCTTGAGCAGCGAGAAGCTGGCACAACCGTGGAGGTTGTTGCCGCACAAACCAAGGCAATTGCAGAGAAAGTGAAAGATTGGACCAATATTGTTTTGGCTTATGAGCCCGTGTGGGCCATTGGAACTGGGAAAGTTGCATCTCCAGCTCAGGCTCAGGAAGTGCATTCTGAATTGAGGAAATGGCTTCAAGCAAAAGTCAGTTCTGAAGTTGCTGCATCAACCCGCATTATTTATGGAGGGTCTGTGAATGGTGCAAACAGCAAAGAGCTGGCTGGTCAGCCTGATATTGACGGATTTTTGGTTGGTGGAGCTTCTCTAAAGCCTGAGTTCATCGACATCATCAAGTCAGCGGAGGTGAAGAAGAGTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00051 ko00562 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00051 map00562 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
TIM

Protein Analysis

255

Amino Acids

27.35

Weight (kDa)

6.02

Isoelectric Point (pI)

32.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIM PF00121 6 - 247 4.6e-91 Triosephosphate isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 218
AciI CCGC 5 cut(s) 24, 332, 435, 616, 743
AcsI RAATTY 1 cut(s) 176
AcuI CTGAAG 1 cut(s) 618
AgsI TTSAA 2 cut(s) 367, 581
AleI CACNNNNGTG 1 cut(s) 419
AluBI AGCT 5 cut(s) 377, 410, 539, 661, 704
AluI AGCT 5 cut(s) 377, 410, 539, 661, 704
Alw26I GTCTC 1 cut(s) 383
Ama87I CYCGRG 1 cut(s) 260
AoxI GGCC 2 cut(s) 170, 507
ApeKI GCWGC 3 cut(s) 188, 400, 605
ApoI RAATTY 1 cut(s) 176
ArsI GACNNNNNNTTYG 2 cut(s) 168, 200
Asp700I GAANNNNTTC 1 cut(s) 14
AspS9I GGNCC 3 cut(s) 171, 474, 507
AsuHPI GGTGA 7 cut(s) 70, 166, 242, 323, 382, 398, 760
AvaI CYCGRG 1 cut(s) 260
AvaII GGWCC 1 cut(s) 474
BanI GGYRCC 1 cut(s) 218
BanII GRGCYC 1 cut(s) 501
BbsI GAAGAC 1 cut(s) 304
BbvI GCAGC 3 cut(s) 175, 412, 592
BccI CCATC 1 cut(s) 106
BcoDI GTCTC 1 cut(s) 383
BfaI CTAG 1 cut(s) 251
BisI GCNGC 5 cut(s) 25, 189, 401, 435, 606
BlsI GCNGC 5 cut(s) 26, 190, 402, 436, 607
Bme18I GGWCC 1 cut(s) 474
BmeT110I CYCGRG 1 cut(s) 260
BmgT120I GGNCC 3 cut(s) 171, 474, 507
BmiI GGNNCC 3 cut(s) 46, 220, 267
BmrI ACTGGG 1 cut(s) 528
BmsI GCATC 3 cut(s) 237, 539, 617
BmuI ACTGGG 1 cut(s) 528
BpiI GAAGAC 1 cut(s) 304
BpmI CTGGAG 1 cut(s) 519
Bpu10I CCTNAGC 2 cut(s) 540, 546
BpuEI CTTGAG 2 cut(s) 342, 416
BsaI GGTCTC 1 cut(s) 383
BsaJI CCNNGG 3 cut(s) 269, 420, 444
BsaWI WCCGGW 1 cut(s) 148
BsaXI ACNNNNNCTCC 2 cut(s) 621, 651
Bse1I ACTGG 3 cut(s) 35, 232, 523
BseDI CCNNGG 3 cut(s) 269, 420, 444
BseMII CTCAG 4 cut(s) 234, 554, 560, 708
BseNI ACTGG 3 cut(s) 35, 232, 523
BseXI GCAGC 3 cut(s) 175, 412, 592
BseYI CCCAGC 1 cut(s) 201
BsgI GTGCAG 1 cut(s) 174
BshFI GGCC 2 cut(s) 172, 509
BshNI GGYRCC 1 cut(s) 218
BsiHKCI CYCGRG 1 cut(s) 260
BsiSI CCGG 1 cut(s) 149
BslFI GGGAC 1 cut(s) 102
BsmAI GTCTC 1 cut(s) 383
BsmFI GGGAC 1 cut(s) 102
BsmI GAATGC 1 cut(s) 556
BsnI GGCC 2 cut(s) 172, 509
Bso31I GGTCTC 1 cut(s) 383
BsoBI CYCGRG 1 cut(s) 260
Bsp1286I GDGCHC 1 cut(s) 501
Bsp143I GATC 1 cut(s) 66
Bsp19I CCATGG 1 cut(s) 269
BspACI CCGC 5 cut(s) 24, 332, 435, 616, 743
BspANI GGCC 2 cut(s) 172, 509
BspCNI CTCAG 4 cut(s) 235, 553, 559, 709
BspLI GGNNCC 3 cut(s) 46, 220, 267
BspT107I GGYRCC 1 cut(s) 218
BspTNI GGTCTC 1 cut(s) 383
BsrI ACTGG 3 cut(s) 35, 232, 523
BssECI CCNNGG 3 cut(s) 269, 420, 444
BssMI GATC 1 cut(s) 66
BssT1I CCWWGG 2 cut(s) 269, 444
Bst4CI ACNGT 2 cut(s) 135, 421
Bst6I CTCTTC 2 cut(s) 152, 749
BstC8I GCNNGC 2 cut(s) 412, 663
BstDEI CTNAG 4 cut(s) 243, 540, 546, 717
BstDSI CCRYGG 2 cut(s) 269, 420
BstKTI GATC 1 cut(s) 69
BstMAI GTCTC 1 cut(s) 383
BstMBI GATC 1 cut(s) 66
BstMWI GCNNNNNNNGC 1 cut(s) 536
BstV1I GCAGC 3 cut(s) 175, 412, 592
BstV2I GAAGAC 1 cut(s) 304
BsuRI GGCC 2 cut(s) 172, 509
BtgI CCRYGG 2 cut(s) 269, 420
BtgZI GCGATG 1 cut(s) 84
BtsIMutI CAGTG 1 cut(s) 225
Cac8I GCNNGC 2 cut(s) 412, 663
Cfr13I GGNCC 3 cut(s) 171, 474, 507
CspCI CAANNNNNGTGG 2 cut(s) 196, 231
CviAII CATG 2 cut(s) 182, 270
DdeI CTNAG 4 cut(s) 243, 540, 546, 717
DpnI GATC 1 cut(s) 68
DpnII GATC 1 cut(s) 66
Eam1104I CTCTTC 2 cut(s) 152, 749
EarI CTCTTC 2 cut(s) 152, 749
Eco130I CCWWGG 2 cut(s) 269, 444
Eco24I GRGCYC 1 cut(s) 501
Eco31I GGTCTC 1 cut(s) 383
Eco47I GGWCC 1 cut(s) 474
Eco57I CTGAAG 1 cut(s) 618
Eco88I CYCGRG 1 cut(s) 260
EcoRI GAATTC 1 cut(s) 176
EcoT14I CCWWGG 2 cut(s) 269, 444
EcoT38I GRGCYC 1 cut(s) 501
ErhI CCWWGG 2 cut(s) 269, 444
FaeI CATG 2 cut(s) 185, 273
FaiI YATR 7 cut(s) 77, 138, 183, 271, 346, 495, 627
FaqI GGGAC 1 cut(s) 102
FatI CATG 2 cut(s) 181, 269
FauI CCCGC 1 cut(s) 623
Fnu4HI GCNGC 5 cut(s) 25, 189, 401, 435, 606
FriOI GRGCYC 1 cut(s) 501
Fsp4HI GCNGC 5 cut(s) 25, 189, 401, 435, 606
FspBI CTAG 1 cut(s) 251
GluI GCNGC 5 cut(s) 25, 189, 401, 435, 606
GsaI CCCAGC 1 cut(s) 205
GsuI CTGGAG 1 cut(s) 519
HaeIII GGCC 2 cut(s) 172, 509
HapII CCGG 1 cut(s) 149
Hin1II CATG 2 cut(s) 185, 273
HinfI GANTC 1 cut(s) 314
HpaII CCGG 1 cut(s) 149
HphI GGTGA 7 cut(s) 70, 166, 242, 323, 382, 398, 760
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 3 cut(s) 292, 562, 598
Hpy188III TCNNGA 4 cut(s) 70, 208, 260, 548
Hpy8I GTNNAC 1 cut(s) 73
Hpy99I CGWCG 1 cut(s) 23
HpyAV CCTTC 4 cut(s) 58, 205, 232, 361
HpyCH4III ACNGT 2 cut(s) 135, 421
HpyCH4V TGCA 8 cut(s) 39, 191, 344, 455, 530, 556, 608, 647
HpyF10VI GCNNNNNNNGC 1 cut(s) 536
HpyF3I CTNAG 4 cut(s) 243, 540, 546, 717
Hsp92II CATG 2 cut(s) 185, 273
Kzo9I GATC 1 cut(s) 66
LmnI GCTCC 1 cut(s) 701
Lsp1109I GCAGC 3 cut(s) 175, 412, 592
LweI GCATC 3 cut(s) 237, 539, 617
MaeI CTAG 1 cut(s) 251
MalI GATC 1 cut(s) 68
MboI GATC 1 cut(s) 66
MboII GAAGA 7 cut(s) 7, 73, 76, 137, 169, 309, 763
MfeI CAATTG 1 cut(s) 450
MhlI GDGCHC 1 cut(s) 501
MluCI AATT 3 cut(s) 176, 450, 563
MnlI CCTC 9 cut(s) 49, 79, 109, 162, 256, 418, 561, 623, 739
MroXI GAANNNNTTC 1 cut(s) 14
MseI TTAA 1 cut(s) 763
MslI CAYNNNNRTG 1 cut(s) 419
MspA1I CMGCKG 1 cut(s) 743
MspI CCGG 1 cut(s) 149
MunI CAATTG 1 cut(s) 450
Mva1269I GAATGC 1 cut(s) 556
MwoI GCNNNNNNNGC 1 cut(s) 536
NcoI CCATGG 1 cut(s) 269
NdeII GATC 1 cut(s) 66
NlaIII CATG 2 cut(s) 185, 273
NlaIV GGNNCC 3 cut(s) 46, 220, 267
OliI CACNNNNGTG 1 cut(s) 419
PaeR7I CTCGAG 1 cut(s) 260
PcsI WCGNNNNNNNCGW 1 cut(s) 48
PctI GAATGC 1 cut(s) 556
PdmI GAANNNNTTC 1 cut(s) 14
PfeI GAWTC 1 cut(s) 314
PkrI GCNGC 5 cut(s) 26, 190, 402, 436, 607
PspFI CCCAGC 1 cut(s) 201
PspN4I GGNNCC 3 cut(s) 46, 220, 267
PspPI GGNCC 3 cut(s) 171, 474, 507
RseI CAYNNNNRTG 1 cut(s) 419
SaqAI TTAA 1 cut(s) 763
SatI GCNGC 5 cut(s) 25, 189, 401, 435, 606
Sau3AI GATC 1 cut(s) 66
Sau96I GGNCC 3 cut(s) 171, 474, 507
SduI GDGCHC 1 cut(s) 501
SfaNI GCATC 3 cut(s) 237, 539, 617
Sfr274I CTCGAG 1 cut(s) 260
SinI GGWCC 1 cut(s) 474
SlaI CTCGAG 1 cut(s) 260
SmiMI CAYNNNNRTG 1 cut(s) 419
SmlI CTYRAG 3 cut(s) 260, 357, 395
SmoI CTYRAG 3 cut(s) 260, 357, 395
Sse9I AATT 3 cut(s) 176, 450, 563
SsiI CCGC 5 cut(s) 24, 332, 435, 616, 743
SspI AATATT 1 cut(s) 481
SspMI CTAG 1 cut(s) 251
StyI CCWWGG 2 cut(s) 269, 444
TaaI ACNGT 2 cut(s) 135, 421
TaqI TCGA 3 cut(s) 51, 261, 726
TasI AATT 3 cut(s) 176, 450, 563
TauI GCSGC 2 cut(s) 27, 437
TfiI GAWTC 1 cut(s) 314
Tru1I TTAA 1 cut(s) 763
Tru9I TTAA 1 cut(s) 763
TscAI CASTG 1 cut(s) 232
TseI GCWGC 3 cut(s) 188, 400, 605
TspDTI ATGAA 2 cut(s) 153, 712
TspGWI ACGGA 2 cut(s) 57, 699
TspRI CASTG 1 cut(s) 232
VpaK11BI GGWCC 1 cut(s) 474
XapI RAATTY 1 cut(s) 176
XcmI CCANNNNNNNNNTGG 1 cut(s) 484
XhoI CTCGAG 1 cut(s) 260
XmnI GAANNNNTTC 1 cut(s) 14
XspI CTAG 1 cut(s) 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.