FvH4_7g22530

triosephosphate isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0007425
Physical Location & Seq
Forward (+)
17724210 .. 17727421
3212 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g22530.t1

Sequence Viewer

Length: 762 bp
ATGGGCCGGAAGTTTTTCGTCGGCGGCAACTGGAAATGCAATGGAACAATCGATGAGGTGAAGAAGATCGTGAACTTGCTCAATGATGGAAAAGTGCCGTCACCAGATTGCGTGGAGGTTGTGGTTAGTCCTCCGTATGTGTTTCTTCCATTGGTGAAGAGCACGTTGAGGCCAGATTTCCAGGTTGCGGCACAGAACTGCTGGGTGAAGAAGGGCGGTGCCTTCACTGGTGAAGTTAGTGCTGAGATGCTTAAAAATCTTGAGGTTCCATGGGTCATCCTTGGTCATTCCGAAAGGAGAGCTCTTTTGGGTGAAACCAATGAGTTTGTTGGGGACAAGGTTGCTTATGCACTTTCCCAAGGTTTGAAGGTGATCGCCTGTGTTGGTGAGACTCTTGAACAGCGAGAAGCTGGATCAACTGTTGAAGTTGTTGCTGCACAAACCAAGGCAATTGCTGAGAAAGTGAAAGATTGGACCAACATTGTTTTGGCTTATGAGCCAGTGTGGGCTATTGGAACCGGGAAGGTTGCATCTCCAGCTCAGGCCCAGGAAGTGCATTGTGAATTGAGGAAATGGCTTCATGCAAACACAAGTCCTGAGGTTGCTGCATCAACCAGGATCATATATGGAGGCTCTGTCAATGGTGCAAACTCCAAAGAGTTGGCAGGTCAGCCTGATCTCGATGGATTTTTGGTTGGTGGTGCTTCTCTAAAGCCCGAGTTTATTGACATTATCAATGCTGCTGTGGTGAAGAAAGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00051 ko00562 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00051 map00562 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
TIM

Protein Analysis

254

Amino Acids

27.13

Weight (kDa)

6.34

Isoelectric Point (pI)

22.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIM PF00121 6 - 248 2.4e-89 Triosephosphate isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 656
AccB1I GGYRCC 1 cut(s) 218
AciI CCGC 3 cut(s) 24, 188, 216
AclWI GGATC 2 cut(s) 421, 626
AfiI CCNNNNNNNGG 1 cut(s) 187
AgsI TTSAA 3 cut(s) 367, 398, 425
AjnI CCWGG 3 cut(s) 180, 546, 614
AluBI AGCT 3 cut(s) 302, 410, 539
AluI AGCT 3 cut(s) 302, 410, 539
Alw21I GWGCWC 2 cut(s) 164, 304
Alw26I GTCTC 1 cut(s) 383
AlwI GGATC 2 cut(s) 421, 626
Ama87I CYCGRG 1 cut(s) 716
AoxI GGCC 3 cut(s) 4, 170, 543
ApeKI GCWGC 3 cut(s) 434, 605, 740
Asp700I GAANNNNTTC 1 cut(s) 14
AspS9I GGNCC 3 cut(s) 4, 474, 544
AsuC2I CCSGG 1 cut(s) 520
AsuHPI GGTGA 9 cut(s) 70, 93, 166, 217, 242, 323, 382, 398, 760
AvaI CYCGRG 1 cut(s) 716
AvaII GGWCC 1 cut(s) 474
AxyI CCTNAGG 1 cut(s) 597
BanI GGYRCC 1 cut(s) 218
BanII GRGCYC 1 cut(s) 304
Bbv12I GWGCWC 2 cut(s) 164, 304
BbvI GCAGC 3 cut(s) 421, 592, 727
BccI CCATC 2 cut(s) 80, 677
BceAI ACGGC 1 cut(s) 82
BciT130I CCWGG 3 cut(s) 182, 548, 616
BcnI CCSGG 1 cut(s) 520
BcoDI GTCTC 1 cut(s) 383
BfaI CTAG 1 cut(s) 760
BfuAI ACCTGC 1 cut(s) 656
BisI GCNGC 5 cut(s) 25, 189, 435, 606, 741
BlsI GCNGC 5 cut(s) 26, 190, 436, 607, 742
Bme1390I CCNGG 4 cut(s) 182, 520, 548, 616
Bme18I GGWCC 1 cut(s) 474
BmeT110I CYCGRG 1 cut(s) 716
BmgT120I GGNCC 3 cut(s) 4, 474, 544
BmiI GGNNCC 3 cut(s) 220, 267, 517
BmrFI CCNGG 4 cut(s) 182, 520, 548, 616
BmsI GCATC 3 cut(s) 237, 539, 617
BpmI CTGGAG 1 cut(s) 519
Bpu10I CCTNAGC 1 cut(s) 540
BpuEI CTTGAG 1 cut(s) 281
BpuMI CCSGG 1 cut(s) 520
Bsa29I ATCGAT 1 cut(s) 51
BsaJI CCNNGG 5 cut(s) 269, 280, 358, 444, 546
BsaXI ACNNNNNCTCC 2 cut(s) 621, 651
Bsc4I CCNNNNNNNGG 1 cut(s) 187
Bse1I ACTGG 3 cut(s) 35, 232, 500
Bse21I CCTNAGG 1 cut(s) 597
Bse3DI GCAATG 1 cut(s) 46
BseBI CCWGG 3 cut(s) 182, 548, 616
BseCI ATCGAT 1 cut(s) 51
BseDI CCNNGG 5 cut(s) 269, 280, 358, 444, 546
BseGI GGATG 1 cut(s) 276
BseLI CCNNNNNNNGG 1 cut(s) 187
BseMI GCAATG 1 cut(s) 46
BseMII CTCAG 4 cut(s) 234, 447, 554, 588
BseNI ACTGG 3 cut(s) 35, 232, 500
BseXI GCAGC 3 cut(s) 421, 592, 727
BseYI CCCAGC 1 cut(s) 201
BsgI GTGCAG 1 cut(s) 420
BshFI GGCC 3 cut(s) 6, 172, 545
BshNI GGYRCC 1 cut(s) 218
BshVI ATCGAT 1 cut(s) 51
BsiHKAI GWGCWC 2 cut(s) 164, 304
BsiHKCI CYCGRG 1 cut(s) 716
BsiSI CCGG 2 cut(s) 7, 519
BslFI GGGAC 1 cut(s) 347
BslI CCNNNNNNNGG 1 cut(s) 187
BsmAI GTCTC 1 cut(s) 383
BsmFI GGGAC 1 cut(s) 347
BsnI GGCC 3 cut(s) 6, 172, 545
BsoBI CYCGRG 1 cut(s) 716
Bsp1286I GDGCHC 2 cut(s) 164, 304
Bsp143I GATC 5 cut(s) 66, 372, 413, 618, 676
Bsp19I CCATGG 1 cut(s) 269
BspACI CCGC 3 cut(s) 24, 188, 216
BspANI GGCC 3 cut(s) 6, 172, 545
BspCNI CTCAG 4 cut(s) 235, 448, 553, 589
BspDI ATCGAT 1 cut(s) 51
BspLI GGNNCC 3 cut(s) 220, 267, 517
BspMI ACCTGC 1 cut(s) 656
BspPI GGATC 2 cut(s) 421, 626
BspQI GCTCTTC 1 cut(s) 152
BspT107I GGYRCC 1 cut(s) 218
BsrDI GCAATG 1 cut(s) 46
BsrI ACTGG 3 cut(s) 35, 232, 500
BssECI CCNNGG 5 cut(s) 269, 280, 358, 444, 546
BssMI GATC 5 cut(s) 66, 372, 413, 618, 676
BssT1I CCWWGG 4 cut(s) 269, 280, 358, 444
Bst2UI CCWGG 3 cut(s) 182, 548, 616
Bst4CI ACNGT 1 cut(s) 421
Bst6I CTCTTC 1 cut(s) 152
BstDEI CTNAG 4 cut(s) 243, 456, 540, 597
BstDSI CCRYGG 1 cut(s) 269
BstF5I GGATG 1 cut(s) 276
BstKTI GATC 5 cut(s) 69, 375, 416, 621, 679
BstMAI GTCTC 1 cut(s) 383
BstMBI GATC 5 cut(s) 66, 372, 413, 618, 676
BstMWI GCNNNNNNNGC 1 cut(s) 536
BstNI CCWGG 3 cut(s) 182, 548, 616
BstSCI CCNGG 4 cut(s) 180, 518, 546, 614
BstV1I GCAGC 3 cut(s) 421, 592, 727
BstXI CCANNNNNNTGG 1 cut(s) 661
Bsu15I ATCGAT 1 cut(s) 51
Bsu36I CCTNAGG 1 cut(s) 597
BsuRI GGCC 3 cut(s) 6, 172, 545
BsuTUI ATCGAT 1 cut(s) 51
BtgI CCRYGG 1 cut(s) 269
BtsCI GGATG 1 cut(s) 276
BtsIMutI CAGTG 2 cut(s) 225, 507
BveI ACCTGC 1 cut(s) 656
Cfr13I GGNCC 3 cut(s) 4, 474, 544
ClaI ATCGAT 1 cut(s) 51
CviAII CATG 2 cut(s) 270, 581
DdeI CTNAG 4 cut(s) 243, 456, 540, 597
DpnI GATC 5 cut(s) 68, 374, 415, 620, 678
DpnII GATC 5 cut(s) 66, 372, 413, 618, 676
Eam1104I CTCTTC 1 cut(s) 152
EarI CTCTTC 1 cut(s) 152
Ecl136II GAGCTC 1 cut(s) 302
Eco130I CCWWGG 4 cut(s) 269, 280, 358, 444
Eco24I GRGCYC 1 cut(s) 304
Eco47I GGWCC 1 cut(s) 474
Eco53kI GAGCTC 1 cut(s) 302
Eco81I CCTNAGG 1 cut(s) 597
Eco88I CYCGRG 1 cut(s) 716
EcoICRI GAGCTC 1 cut(s) 302
EcoRII CCWGG 3 cut(s) 180, 546, 614
EcoT14I CCWWGG 4 cut(s) 269, 280, 358, 444
EcoT38I GRGCYC 1 cut(s) 304
ErhI CCWWGG 4 cut(s) 269, 280, 358, 444
FaeI CATG 2 cut(s) 273, 584
FaiI YATR 8 cut(s) 138, 271, 348, 495, 582, 623, 625, 627
FaqI GGGAC 1 cut(s) 347
FatI CATG 2 cut(s) 269, 580
Fnu4HI GCNGC 5 cut(s) 25, 189, 435, 606, 741
FokI GGATG 1 cut(s) 263
FriOI GRGCYC 1 cut(s) 304
Fsp4HI GCNGC 5 cut(s) 25, 189, 435, 606, 741
FspBI CTAG 1 cut(s) 760
GluI GCNGC 5 cut(s) 25, 189, 435, 606, 741
GsaI CCCAGC 1 cut(s) 205
GsuI CTGGAG 1 cut(s) 519
HaeIII GGCC 3 cut(s) 6, 172, 545
HapII CCGG 2 cut(s) 7, 519
Hin1II CATG 2 cut(s) 273, 584
HinfI GANTC 1 cut(s) 391
HpaII CCGG 2 cut(s) 7, 519
HphI GGTGA 9 cut(s) 70, 93, 166, 217, 242, 323, 382, 398, 760
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 1 cut(s) 292
Hpy188III TCNNGA 5 cut(s) 70, 260, 395, 596, 680
Hpy8I GTNNAC 1 cut(s) 73
Hpy99I CGWCG 1 cut(s) 23
HpyAV CCTTC 4 cut(s) 205, 232, 361, 517
HpyCH4III ACNGT 1 cut(s) 421
HpyCH4IV ACGT 1 cut(s) 164
HpyCH4V TGCA 8 cut(s) 39, 350, 437, 530, 556, 584, 608, 647
HpyF10VI GCNNNNNNNGC 1 cut(s) 536
HpyF3I CTNAG 4 cut(s) 243, 456, 540, 597
HpySE526I ACGT 1 cut(s) 164
Hsp92II CATG 2 cut(s) 273, 584
Kzo9I GATC 5 cut(s) 66, 372, 413, 618, 676
LguI GCTCTTC 1 cut(s) 152
Lsp1109I GCAGC 3 cut(s) 421, 592, 727
LweI GCATC 3 cut(s) 237, 539, 617
MaeI CTAG 1 cut(s) 760
MaeII ACGT 1 cut(s) 164
MaeIII GTNAC 1 cut(s) 99
MalI GATC 5 cut(s) 68, 374, 415, 620, 678
MboI GATC 5 cut(s) 66, 372, 413, 618, 676
MboII GAAGA 5 cut(s) 73, 76, 137, 169, 220
MfeI CAATTG 1 cut(s) 450
MhlI GDGCHC 2 cut(s) 164, 304
MluCI AATT 2 cut(s) 450, 563
MlyI GAGTC 1 cut(s) 385
MnlI CCTC 8 cut(s) 49, 109, 141, 162, 256, 561, 592, 623
MroXI GAANNNNTTC 1 cut(s) 14
MseI TTAA 1 cut(s) 252
MspI CCGG 2 cut(s) 7, 519
MspR9I CCNGG 4 cut(s) 182, 520, 548, 616
MunI CAATTG 1 cut(s) 450
MvaI CCWGG 3 cut(s) 182, 548, 616
MwoI GCNNNNNNNGC 1 cut(s) 536
NciI CCSGG 1 cut(s) 520
NcoI CCATGG 1 cut(s) 269
NdeII GATC 5 cut(s) 66, 372, 413, 618, 676
NlaIII CATG 2 cut(s) 273, 584
NlaIV GGNNCC 3 cut(s) 220, 267, 517
NmuCI GTSAC 1 cut(s) 99
PciSI GCTCTTC 1 cut(s) 152
PdmI GAANNNNTTC 1 cut(s) 14
PkrI GCNGC 5 cut(s) 26, 190, 436, 607, 742
PleI GAGTC 1 cut(s) 385
PpsI GAGTC 1 cut(s) 385
Psp124BI GAGCTC 1 cut(s) 304
Psp6I CCWGG 3 cut(s) 180, 546, 614
PspFI CCCAGC 1 cut(s) 201
PspGI CCWGG 3 cut(s) 180, 546, 614
PspN4I GGNNCC 3 cut(s) 220, 267, 517
PspPI GGNCC 3 cut(s) 4, 474, 544
SacI GAGCTC 1 cut(s) 304
SapI GCTCTTC 1 cut(s) 152
SaqAI TTAA 1 cut(s) 252
SatI GCNGC 5 cut(s) 25, 189, 435, 606, 741
Sau3AI GATC 5 cut(s) 66, 372, 413, 618, 676
Sau96I GGNCC 3 cut(s) 4, 474, 544
SchI GAGTC 1 cut(s) 385
ScrFI CCNGG 4 cut(s) 182, 520, 548, 616
SduI GDGCHC 2 cut(s) 164, 304
SfaNI GCATC 3 cut(s) 237, 539, 617
SinI GGWCC 1 cut(s) 474
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
Sse9I AATT 2 cut(s) 450, 563
SsiI CCGC 3 cut(s) 24, 188, 216
SspMI CTAG 1 cut(s) 760
SstI GAGCTC 1 cut(s) 304
StyD4I CCNGG 4 cut(s) 180, 518, 546, 614
StyI CCWWGG 4 cut(s) 269, 280, 358, 444
TaaI ACNGT 1 cut(s) 421
TaiI ACGT 1 cut(s) 167
TaqI TCGA 2 cut(s) 51, 681
TasI AATT 2 cut(s) 450, 563
TauI GCSGC 2 cut(s) 27, 191
Tru1I TTAA 1 cut(s) 252
Tru9I TTAA 1 cut(s) 252
TscAI CASTG 2 cut(s) 232, 507
TseFI GTSAC 1 cut(s) 99
TseI GCWGC 3 cut(s) 434, 605, 740
Tsp45I GTSAC 1 cut(s) 99
TspDTI ATGAA 1 cut(s) 569
TspGWI ACGGA 1 cut(s) 123
TspRI CASTG 2 cut(s) 232, 507
VpaK11BI GGWCC 1 cut(s) 474
XcmI CCANNNNNNNNNTGG 1 cut(s) 484
XmnI GAANNNNTTC 1 cut(s) 14
XspI CTAG 1 cut(s) 760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.