MD00G1012200.v1.1

Triosephosphate isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0007425
Physical Location & Seq
Forward (+)
1510464 .. 1514078
3615 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1012200.v1.1.491

Sequence Viewer

Length: 780 bp
ATGGGCAGAAAATTCTTCGTGGGTGGCAACTGGAAATGCAATGGGACAACTGCAGAGGTGAAGAAGATTGTTACTTCATTGAATGAAGCTGAAGTCCCTTCAGAAGATGTTGTGGAGGTTGTGGTGAGCCCTCCGTTTGTGTTTCTTTCTTTAGTGAAAAGTTTATTGCGGTCTGATTTCAATGTCGCGGCACAGAACTGTTGGGTGCGGAAAGGTGGTGCTTTTACCGGAGAGGTTAGTGTGGAGATGCTGGTGAATTTGGGCATTCCCTGGGTAATTATTGGTCACTCTGAAAGAAGACTTATTCTAAATGAGTCCAATGAGTTTGTTGGGGATAAGGTTTCTTATGCACTTTCTCAAGGCTTAAAGGTTATAGCTTGTGTTGGGGAGACTCTTGAGCAGAGAGAATCAGGATCTACAGTGGCTGTTGTTTCCGCGCAAACAAAAGCAATCGCAGATAAAGTATCGAGCTGGGAGAACATTGTTTTGGCTTATGAGCCAGTTTGGGCAATTGGAACTGGGAAGGTTGCAAGCCCTGCTCAGGCTCAGGAAGATGCTGTAGCTCAGGTCCATTTTGAATTGAGGAAATGGCTTCATGACAATGTTTGTGCTGAAGTTGCTGCATCAACCAGAATCATTTATGGAGGTTCTGTAAATGGAGCAAACTGCACGGAGTTGGCAACACAGCCAGATATGGATGGATTTTTGGTTGGCGGAGCTTCCCTTAAGCCGGAGTTCATTGACATTATCGAGTCAGCTACAGTGAAGAAGAATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004807 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0005911 GO:0005975 GO:0005996 GO:0006006 GO:0006066 GO:0006071 GO:0006081 GO:0006082 GO:0006090 GO:0006091 GO:0006094 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009506 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010043 GO:0016020 GO:0016051 GO:0016052 GO:0016053 GO:0016310 GO:0016853 GO:0016860 GO:0016861 GO:0017144 GO:0018130 GO:0019318 GO:0019319 GO:0019359 GO:0019362 GO:0019363 GO:0019400 GO:0019405 GO:0019438 GO:0019439 GO:0019563 GO:0019637 GO:0019682 GO:0019693 GO:0019751 GO:0019752 GO:0030054 GO:0030312 GO:0031090 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042221 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044275 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046164 GO:0046166 GO:0046174 GO:0046184 GO:0046364 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046872 GO:0046914 GO:0046939 GO:0048046 GO:0050896 GO:0051186 GO:0051188 GO:0055044 GO:0055086 GO:0071704 GO:0071944 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:0098588 GO:0098805 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1901615 GO:1901616
KEGG Pathways
Metabolic & Signaling
ko00010 ko00051 ko00562 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00051 map00562 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
TIM

Protein Analysis

260

Amino Acids

27.84

Weight (kDa)

5.05

Isoelectric Point (pI)

29.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIM PF00121 6 - 252 4.4e-87 Triosephosphate isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 188, 437
AciI CCGC 5 cut(s) 169, 188, 208, 435, 714
AclWI GGATC 1 cut(s) 421
AcsI RAATTY 2 cut(s) 11, 256
AcuI CTGAAG 3 cut(s) 84, 111, 633
AfiI CCNNNNNNNGG 2 cut(s) 541, 730
AflII CTTAAG 1 cut(s) 725
AgsI TTSAA 3 cut(s) 82, 181, 578
AjnI CCWGG 1 cut(s) 269
AjuI GAANNNNNNNTTGG 2 cut(s) 470, 502
AluBI AGCT 6 cut(s) 89, 377, 471, 563, 719, 758
AluI AGCT 6 cut(s) 89, 377, 471, 563, 719, 758
Alw26I GTCTC 1 cut(s) 383
AlwI GGATC 1 cut(s) 421
AlwNI CAGNNNCTG 1 cut(s) 425
ApeKI GCWGC 1 cut(s) 620
ApoI RAATTY 2 cut(s) 11, 256
AspLEI GCGC 1 cut(s) 439
AspS9I GGNCC 1 cut(s) 568
AsuHPI GGTGA 3 cut(s) 70, 136, 265
AvaII GGWCC 1 cut(s) 568
BanII GRGCYC 1 cut(s) 131
BbsI GAAGAC 1 cut(s) 304
BbvI GCAGC 1 cut(s) 607
BccI CCATC 1 cut(s) 692
BciT130I CCWGG 1 cut(s) 271
BcoDI GTCTC 1 cut(s) 383
BfmI CTRYAG 4 cut(s) 51, 417, 558, 759
BfrI CTTAAG 1 cut(s) 725
BisI GCNGC 2 cut(s) 189, 621
BlsI GCNGC 2 cut(s) 190, 622
Bme1390I CCNGG 1 cut(s) 271
Bme18I GGWCC 1 cut(s) 568
BmgT120I GGNCC 1 cut(s) 568
BmrFI CCNGG 1 cut(s) 271
BmrI ACTGGG 1 cut(s) 528
BmsI GCATC 3 cut(s) 237, 544, 632
BmuI ACTGGG 1 cut(s) 528
BpiI GAAGAC 1 cut(s) 304
Bpu10I CCTNAGC 3 cut(s) 540, 546, 564
BpuEI CTTGAG 2 cut(s) 342, 416
BsaJI CCNNGG 2 cut(s) 269, 270
BsaWI WCCGGW 1 cut(s) 227
BsaXI ACNNNNNCTCC 2 cut(s) 636, 666
Bsc4I CCNNNNNNNGG 2 cut(s) 541, 730
Bse1I ACTGG 3 cut(s) 35, 500, 523
Bse3DI GCAATG 1 cut(s) 46
BseBI CCWGG 1 cut(s) 271
BseDI CCNNGG 2 cut(s) 269, 270
BseGI GGATG 1 cut(s) 703
BseLI CCNNNNNNNGG 2 cut(s) 541, 730
BseMI GCAATG 1 cut(s) 46
BseMII CTCAG 3 cut(s) 554, 560, 578
BseNI ACTGG 3 cut(s) 35, 500, 523
BseXI GCAGC 1 cut(s) 607
BseYI CCCAGC 1 cut(s) 471
BsgI GTGCAG 1 cut(s) 652
Bsh1236I CGCG 2 cut(s) 188, 437
BsiSI CCGG 2 cut(s) 228, 731
BslFI GGGAC 2 cut(s) 58, 80
BslI CCNNNNNNNGG 2 cut(s) 541, 730
BsmAI GTCTC 1 cut(s) 383
BsmFI GGGAC 2 cut(s) 58, 80
BsmI GAATGC 1 cut(s) 264
Bsp1286I GDGCHC 1 cut(s) 131
Bsp143I GATC 1 cut(s) 413
BspACI CCGC 5 cut(s) 169, 188, 208, 435, 714
BspCNI CTCAG 3 cut(s) 553, 559, 577
BspFNI CGCG 2 cut(s) 188, 437
BspHI TCATGA 1 cut(s) 595
BspMAI CTGCAG 1 cut(s) 55
BspPI GGATC 1 cut(s) 421
BspTI CTTAAG 1 cut(s) 725
BsrDI GCAATG 1 cut(s) 46
BsrI ACTGG 3 cut(s) 35, 500, 523
BssECI CCNNGG 2 cut(s) 269, 270
BssMI GATC 1 cut(s) 413
Bst2UI CCWGG 1 cut(s) 271
Bst4CI ACNGT 3 cut(s) 200, 421, 763
BstAFI CTTAAG 1 cut(s) 725
BstAPI GCANNNNNTGC 1 cut(s) 536
BstC8I GCNNGC 1 cut(s) 532
BstDEI CTNAG 3 cut(s) 540, 546, 564
BstF5I GGATG 1 cut(s) 703
BstFNI CGCG 2 cut(s) 188, 437
BstHHI GCGC 1 cut(s) 439
BstKTI GATC 1 cut(s) 416
BstMAI GTCTC 1 cut(s) 383
BstMBI GATC 1 cut(s) 413
BstMWI GCNNNNNNNGC 2 cut(s) 536, 617
BstNI CCWGG 1 cut(s) 271
BstSCI CCNGG 1 cut(s) 269
BstSFI CTRYAG 4 cut(s) 51, 417, 558, 759
BstUI CGCG 2 cut(s) 188, 437
BstV1I GCAGC 1 cut(s) 607
BstV2I GAAGAC 1 cut(s) 304
BstX2I RGATCY 1 cut(s) 413
BstYI RGATCY 1 cut(s) 413
BtsCI GGATG 1 cut(s) 703
BtsIMutI CAGTG 2 cut(s) 426, 768
Cac8I GCNNGC 1 cut(s) 532
CaiI CAGNNNCTG 1 cut(s) 425
CciI TCATGA 1 cut(s) 595
CfoI GCGC 1 cut(s) 439
Cfr13I GGNCC 1 cut(s) 568
CviAII CATG 1 cut(s) 596
DdeI CTNAG 3 cut(s) 540, 546, 564
DpnI GATC 1 cut(s) 415
DpnII GATC 1 cut(s) 413
EciI GGCGGA 1 cut(s) 729
Eco24I GRGCYC 1 cut(s) 131
Eco47I GGWCC 1 cut(s) 568
Eco57I CTGAAG 3 cut(s) 84, 111, 633
EcoRII CCWGG 1 cut(s) 269
EcoT38I GRGCYC 1 cut(s) 131
FaeI CATG 1 cut(s) 599
FaiI YATR 6 cut(s) 348, 374, 495, 597, 642, 695
FaqI GGGAC 2 cut(s) 58, 80
FatI CATG 1 cut(s) 595
Fnu4HI GCNGC 2 cut(s) 189, 621
FokI GGATG 1 cut(s) 710
FriOI GRGCYC 1 cut(s) 131
Fsp4HI GCNGC 2 cut(s) 189, 621
GlaI GCGC 1 cut(s) 438
GluI GCNGC 2 cut(s) 189, 621
GsaI CCCAGC 1 cut(s) 475
HapII CCGG 2 cut(s) 228, 731
HhaI GCGC 1 cut(s) 439
Hin1II CATG 1 cut(s) 599
Hin6I GCGC 1 cut(s) 437
HinP1I GCGC 1 cut(s) 437
HinfI GANTC 5 cut(s) 314, 391, 407, 633, 752
HpaII CCGG 2 cut(s) 228, 731
HphI GGTGA 3 cut(s) 70, 136, 265
Hpy188I TCNGA 3 cut(s) 103, 175, 292
Hpy188III TCNNGA 4 cut(s) 395, 411, 548, 596
HpyAV CCTTC 2 cut(s) 108, 517
HpyCH4III ACNGT 3 cut(s) 200, 421, 763
HpyCH4V TGCA 6 cut(s) 39, 53, 350, 530, 623, 669
HpyF10VI GCNNNNNNNGC 2 cut(s) 536, 617
HpyF3I CTNAG 3 cut(s) 540, 546, 564
Hsp92II CATG 1 cut(s) 599
HspAI GCGC 1 cut(s) 437
Kzo9I GATC 1 cut(s) 413
LmnI GCTCC 2 cut(s) 659, 716
Lsp1109I GCAGC 1 cut(s) 607
LweI GCATC 3 cut(s) 237, 544, 632
MaeIII GTNAC 2 cut(s) 70, 284
MalI GATC 1 cut(s) 415
MboI GATC 1 cut(s) 413
MboII GAAGA 7 cut(s) 7, 73, 76, 116, 309, 563, 778
MfeI CAATTG 1 cut(s) 510
MflI RGATCY 1 cut(s) 413
MhlI GDGCHC 1 cut(s) 131
MluCI AATT 5 cut(s) 11, 256, 276, 510, 578
MlyI GAGTC 3 cut(s) 323, 385, 761
MnlI CCTC 6 cut(s) 49, 109, 141, 226, 576, 638
MseI TTAA 2 cut(s) 365, 726
MslI CAYNNNNRTG 1 cut(s) 600
MspCI CTTAAG 1 cut(s) 725
MspI CCGG 2 cut(s) 228, 731
MspR9I CCNGG 1 cut(s) 271
MunI CAATTG 1 cut(s) 510
Mva1269I GAATGC 1 cut(s) 264
MvaI CCWGG 1 cut(s) 271
MvnI CGCG 2 cut(s) 188, 437
MwoI GCNNNNNNNGC 2 cut(s) 536, 617
NdeII GATC 1 cut(s) 413
NlaIII CATG 1 cut(s) 599
NmuCI GTSAC 1 cut(s) 284
PagI TCATGA 1 cut(s) 595
PasI CCCWGGG 1 cut(s) 270
PctI GAATGC 1 cut(s) 264
PfeI GAWTC 2 cut(s) 407, 633
PkrI GCNGC 2 cut(s) 190, 622
PleI GAGTC 3 cut(s) 322, 385, 760
PpsI GAGTC 3 cut(s) 322, 385, 760
Psp6I CCWGG 1 cut(s) 269
PspFI CCCAGC 1 cut(s) 471
PspGI CCWGG 1 cut(s) 269
PspPI GGNCC 1 cut(s) 568
PstI CTGCAG 1 cut(s) 55
PstNI CAGNNNCTG 1 cut(s) 425
PsuI RGATCY 1 cut(s) 413
RseI CAYNNNNRTG 1 cut(s) 600
SaqAI TTAA 2 cut(s) 365, 726
SatI GCNGC 2 cut(s) 189, 621
Sau3AI GATC 1 cut(s) 413
Sau96I GGNCC 1 cut(s) 568
SchI GAGTC 3 cut(s) 323, 385, 761
ScrFI CCNGG 1 cut(s) 271
SduI GDGCHC 1 cut(s) 131
SfaNI GCATC 3 cut(s) 237, 544, 632
SfcI CTRYAG 4 cut(s) 51, 417, 558, 759
SinI GGWCC 1 cut(s) 568
SmiMI CAYNNNNRTG 1 cut(s) 600
SmlI CTYRAG 3 cut(s) 357, 395, 725
SmoI CTYRAG 3 cut(s) 357, 395, 725
Sse9I AATT 5 cut(s) 11, 256, 276, 510, 578
SsiI CCGC 5 cut(s) 169, 188, 208, 435, 714
StyD4I CCNGG 1 cut(s) 269
TaaI ACNGT 3 cut(s) 200, 421, 763
TaqI TCGA 2 cut(s) 467, 750
TasI AATT 5 cut(s) 11, 256, 276, 510, 578
TauI GCSGC 1 cut(s) 191
TfiI GAWTC 2 cut(s) 407, 633
Tru1I TTAA 2 cut(s) 365, 726
Tru9I TTAA 2 cut(s) 365, 726
TscAI CASTG 2 cut(s) 426, 768
TseFI GTSAC 1 cut(s) 284
TseI GCWGC 1 cut(s) 620
Tsp45I GTSAC 1 cut(s) 284
TspDTI ATGAA 4 cut(s) 66, 99, 584, 727
TspGWI ACGGA 2 cut(s) 123, 686
TspRI CASTG 2 cut(s) 426, 768
Vha464I CTTAAG 1 cut(s) 725
VpaK11BI GGWCC 1 cut(s) 568
XapI RAATTY 2 cut(s) 11, 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.