Rorug01G0335500

triosephosphate isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0007425
Physical Location & Seq
Reverse (-)
45385035 .. 45386335
1301 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0335500.1

Sequence Viewer

Length: 864 bp
ATGTCGATCATCGACGCTGTCACTGCCTCATTTTGCGAATTTCCTGGCCCTCGTTGGCGGCGAGGTGATCGACATCTCAACGATAGTCAGCGGCTCTCATCGCCGTGCGTCGCAGTCGTACCTCCTGGCTCATCCCTTGGCAGCGAAACCTGCTGCTCTGAACGATATGAAACCTTCTTTAAGCGTGTTTGGGTGCTTGATAGGGATTTCAGGATCCAGGAGAGGGTACCAAATACATTTGTGCTAAGTTTTGATCTACTTCGGGACAGGAACAAGGTGTTGAAAGGGGGACCTTGGAGATTCAATCATGCTCCCGTGGTTCTACGTGAATATGATGGCATTACACCAGTGCAAACAGTAGATCTCGTATCGAAGACATTCCACCGTTGGATGTTGCCCTGCGGCTCATGGCAGGGCGGCACCAAGGCTGGAACAAGCGGCCTGGAAAAGAGACCTGAAATCCCGAGTTCTTCTTTGGGTTTTGCTAACATCAGGGACAAACCTGCTTCCCTGTATTCATCACCAAAGAAATTGAAGGTATTGTTGCCAGACTTATTTAGCACACCAACATGCATGGATATGCCTACAAAGAAGTTGAAGATGTCAGAATTCACTACCAAGTTCACTGCTAAATCACTTGGGATGGTTGAAGCTCCTGGGAGTATTTTAGTGCCTAAAGAGTATGTGCATGAGGTGATGACTGAACCAAATAAGAAAAGAGGAAGGCCTCTTGGGTCCAAGAATAAGAATCCTTCCAAAAGCAAAGAAAGTAGCGGAAGACAGGAACTCTGCCTGGCCTATCTTACCAAGCTTCCAATCCCTAGTTTGAAGGGCAAGGAGAAAATTGAAGTCTTTTATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00051 ko00562 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00051 map00562 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
TIM

Protein Analysis

287

Amino Acids

32.34

Weight (kDa)

9.67

Isoelectric Point (pI)

57.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 56 - 111 1.4e-07 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 158, 511
Acc65I GGTACC 1 cut(s) 226
AccB1I GGYRCC 2 cut(s) 226, 419
AciI CCGC 6 cut(s) 58, 91, 402, 417, 438, 774
AclWI GGATC 2 cut(s) 208, 221
AcsI RAATTY 2 cut(s) 38, 608
AfaI GTAC 2 cut(s) 120, 228
AfiI CCNNNNNNNGG 1 cut(s) 223
AgsI TTSAA 7 cut(s) 283, 304, 535, 598, 650, 829, 848
AjnI CCWGG 6 cut(s) 43, 124, 216, 441, 655, 792
AluBI AGCT 2 cut(s) 653, 811
AluI AGCT 2 cut(s) 653, 811
Alw26I GTCTC 1 cut(s) 445
AlwI GGATC 2 cut(s) 208, 221
Ama87I CYCGRG 1 cut(s) 463
AoxI GGCC 4 cut(s) 46, 439, 725, 795
ApeKI GCWGC 2 cut(s) 141, 153
ApoI RAATTY 2 cut(s) 38, 608
Asp700I GAANNNNTTC 1 cut(s) 377
Asp718I GGTACC 1 cut(s) 226
AspS9I GGNCC 3 cut(s) 47, 290, 735
AsuHPI GGTGA 3 cut(s) 77, 513, 706
AvaI CYCGRG 1 cut(s) 463
AvaII GGWCC 2 cut(s) 290, 735
BamHI GGATCC 1 cut(s) 213
BanI GGYRCC 2 cut(s) 226, 419
BbsI GAAGAC 2 cut(s) 380, 784
BbvI GCAGC 2 cut(s) 140, 153
BccI CCATC 2 cut(s) 329, 637
BceAI ACGGC 1 cut(s) 88
BciT130I CCWGG 6 cut(s) 45, 126, 218, 443, 657, 794
BcoDI GTCTC 1 cut(s) 445
BfaI CTAG 1 cut(s) 822
BfuAI ACCTGC 2 cut(s) 158, 511
BglII AGATCT 1 cut(s) 361
BisI GCNGC 7 cut(s) 59, 92, 142, 154, 403, 418, 439
BlsI GCNGC 7 cut(s) 60, 93, 143, 155, 404, 419, 440
Bme1390I CCNGG 6 cut(s) 45, 126, 218, 443, 657, 794
Bme18I GGWCC 2 cut(s) 290, 735
BmeT110I CYCGRG 1 cut(s) 463
BmgT120I GGNCC 3 cut(s) 47, 290, 735
BmiI GGNNCC 5 cut(s) 215, 228, 291, 421, 736
BmrFI CCNGG 6 cut(s) 45, 126, 218, 443, 657, 794
BpiI GAAGAC 2 cut(s) 380, 784
BsaAI YACGTR 1 cut(s) 326
BsaBI GATNNNNATC 1 cut(s) 72
BsaI GGTCTC 1 cut(s) 445
BsaJI CCNNGG 5 cut(s) 136, 293, 315, 423, 656
Bsc4I CCNNNNNNNGG 1 cut(s) 223
Bse1I ACTGG 1 cut(s) 347
Bse8I GATNNNNATC 1 cut(s) 72
BseBI CCWGG 6 cut(s) 45, 126, 218, 443, 657, 794
BseDI CCNNGG 5 cut(s) 136, 293, 315, 423, 656
BseGI GGATG 3 cut(s) 131, 396, 648
BseJI GATNNNNATC 1 cut(s) 72
BseLI CCNNNNNNNGG 1 cut(s) 223
BseNI ACTGG 1 cut(s) 347
BseXI GCAGC 2 cut(s) 140, 153
BshFI GGCC 4 cut(s) 48, 441, 727, 797
BshNI GGYRCC 2 cut(s) 226, 419
BsiHKCI CYCGRG 1 cut(s) 463
BslFI GGGAC 3 cut(s) 278, 303, 509
BslI CCNNNNNNNGG 1 cut(s) 223
BsmAI GTCTC 1 cut(s) 445
BsmFI GGGAC 3 cut(s) 278, 303, 509
BsnI GGCC 4 cut(s) 48, 441, 727, 797
Bso31I GGTCTC 1 cut(s) 445
BsoBI CYCGRG 1 cut(s) 463
Bsp143I GATC 5 cut(s) 6, 67, 213, 253, 361
BspACI CCGC 6 cut(s) 58, 91, 402, 417, 438, 774
BspANI GGCC 4 cut(s) 48, 441, 727, 797
BspLI GGNNCC 5 cut(s) 215, 228, 291, 421, 736
BspMI ACCTGC 2 cut(s) 158, 511
BspPI GGATC 2 cut(s) 208, 221
BspT107I GGYRCC 2 cut(s) 226, 419
BspTNI GGTCTC 1 cut(s) 445
BsrI ACTGG 1 cut(s) 347
BssECI CCNNGG 5 cut(s) 136, 293, 315, 423, 656
BssMI GATC 5 cut(s) 6, 67, 213, 253, 361
BssT1I CCWWGG 3 cut(s) 136, 293, 423
Bst2UI CCWGG 6 cut(s) 45, 126, 218, 443, 657, 794
Bst4CI ACNGT 2 cut(s) 358, 386
BstBAI YACGTR 1 cut(s) 326
BstDEI CTNAG 1 cut(s) 245
BstDSI CCRYGG 1 cut(s) 315
BstF5I GGATG 3 cut(s) 131, 396, 648
BstKTI GATC 5 cut(s) 9, 70, 216, 256, 364
BstMAI GTCTC 1 cut(s) 445
BstMBI GATC 5 cut(s) 6, 67, 213, 253, 361
BstMWI GCNNNNNNNGC 3 cut(s) 23, 100, 150
BstNI CCWGG 6 cut(s) 45, 126, 218, 443, 657, 794
BstNSI RCATGY 1 cut(s) 573
BstSCI CCNGG 6 cut(s) 43, 124, 216, 441, 655, 792
BstV1I GCAGC 2 cut(s) 140, 153
BstV2I GAAGAC 2 cut(s) 380, 784
BstX2I RGATCY 2 cut(s) 213, 361
BstYI RGATCY 2 cut(s) 213, 361
BsuRI GGCC 4 cut(s) 48, 441, 727, 797
BtgI CCRYGG 1 cut(s) 315
BtgZI GCGATG 1 cut(s) 84
BtsCI GGATG 3 cut(s) 131, 396, 648
BtsI GCAGTG 2 cut(s) 21, 624
BtsIMutI CAGTG 3 cut(s) 21, 354, 624
BveI ACCTGC 2 cut(s) 158, 511
Cfr13I GGNCC 3 cut(s) 47, 290, 735
CseI GACGC 2 cut(s) 23, 97
Csp6I GTAC 2 cut(s) 119, 227
CviAII CATG 5 cut(s) 308, 408, 570, 574, 689
CviQI GTAC 2 cut(s) 119, 227
DdeI CTNAG 1 cut(s) 245
DpnI GATC 5 cut(s) 8, 69, 215, 255, 363
DpnII GATC 5 cut(s) 6, 67, 213, 253, 361
Eco130I CCWWGG 3 cut(s) 136, 293, 423
Eco147I AGGCCT 1 cut(s) 727
Eco31I GGTCTC 1 cut(s) 445
Eco47I GGWCC 2 cut(s) 290, 735
Eco88I CYCGRG 1 cut(s) 463
EcoO109I RGGNCCY 1 cut(s) 290
EcoRI GAATTC 1 cut(s) 608
EcoRII CCWGG 6 cut(s) 43, 124, 216, 441, 655, 792
EcoT14I CCWWGG 3 cut(s) 136, 293, 423
EcoT22I ATGCAT 1 cut(s) 575
ErhI CCWWGG 3 cut(s) 136, 293, 423
FaeI CATG 5 cut(s) 311, 411, 573, 577, 692
FaiI YATR 9 cut(s) 168, 309, 333, 409, 571, 575, 581, 684, 690
FaqI GGGAC 3 cut(s) 278, 303, 509
FatI CATG 5 cut(s) 307, 407, 569, 573, 688
Fnu4HI GCNGC 7 cut(s) 59, 92, 142, 154, 403, 418, 439
FokI GGATG 3 cut(s) 118, 403, 655
Fsp4HI GCNGC 7 cut(s) 59, 92, 142, 154, 403, 418, 439
FspBI CTAG 1 cut(s) 822
GluI GCNGC 7 cut(s) 59, 92, 142, 154, 403, 418, 439
HaeIII GGCC 4 cut(s) 48, 441, 727, 797
HgaI GACGC 2 cut(s) 23, 97
Hin1II CATG 5 cut(s) 311, 411, 573, 577, 692
HindIII AAGCTT 1 cut(s) 809
HinfI GANTC 2 cut(s) 300, 748
HphI GGTGA 3 cut(s) 77, 513, 706
Hpy166II GTNNAC 1 cut(s) 624
Hpy188I TCNGA 2 cut(s) 160, 607
Hpy188III TCNNGA 3 cut(s) 211, 263, 463
Hpy8I GTNNAC 1 cut(s) 624
Hpy99I CGWCG 2 cut(s) 17, 113
HpyAV CCTTC 5 cut(s) 184, 529, 717, 762, 823
HpyCH4III ACNGT 2 cut(s) 358, 386
HpyCH4IV ACGT 1 cut(s) 325
HpyCH4V TGCA 3 cut(s) 352, 573, 688
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 100, 150
HpyF3I CTNAG 1 cut(s) 245
HpySE526I ACGT 1 cut(s) 325
Hsp92II CATG 5 cut(s) 311, 411, 573, 577, 692
KpnI GGTACC 1 cut(s) 230
Kzo9I GATC 5 cut(s) 6, 67, 213, 253, 361
LmnI GCTCC 2 cut(s) 316, 658
Lsp1109I GCAGC 2 cut(s) 140, 153
MaeI CTAG 1 cut(s) 822
MaeII ACGT 1 cut(s) 325
MaeIII GTNAC 1 cut(s) 19
MalI GATC 5 cut(s) 8, 69, 215, 255, 363
MboI GATC 5 cut(s) 6, 67, 213, 253, 361
MboII GAAGA 4 cut(s) 385, 462, 610, 789
MflI RGATCY 2 cut(s) 213, 361
MluCI AATT 4 cut(s) 38, 530, 608, 843
MmeI TCCRAC 1 cut(s) 368
MnlI CCTC 8 cut(s) 37, 56, 60, 132, 216, 685, 713, 738
Mph1103I ATGCAT 1 cut(s) 575
MroXI GAANNNNTTC 1 cut(s) 377
MseI TTAA 1 cut(s) 180
MslI CAYNNNNRTG 3 cut(s) 103, 568, 578
MspA1I CMGCKG 1 cut(s) 91
MspR9I CCNGG 6 cut(s) 45, 126, 218, 443, 657, 794
MvaI CCWGG 6 cut(s) 45, 126, 218, 443, 657, 794
MwoI GCNNNNNNNGC 3 cut(s) 23, 100, 150
NdeII GATC 5 cut(s) 6, 67, 213, 253, 361
NlaIII CATG 5 cut(s) 311, 411, 573, 577, 692
NlaIV GGNNCC 5 cut(s) 215, 228, 291, 421, 736
NmuCI GTSAC 1 cut(s) 19
NsiI ATGCAT 1 cut(s) 575
NspI RCATGY 1 cut(s) 573
PceI AGGCCT 1 cut(s) 727
PcsI WCGNNNNNNNCGW 1 cut(s) 58
PdmI GAANNNNTTC 1 cut(s) 377
PfeI GAWTC 2 cut(s) 300, 748
PflFI GACNNNGTC 1 cut(s) 17
PfoI TCCNGGA 1 cut(s) 216
PkrI GCNGC 7 cut(s) 60, 93, 143, 155, 404, 419, 440
Ppu21I YACGTR 1 cut(s) 326
PpuMI RGGWCCY 1 cut(s) 290
Psp5II RGGWCCY 1 cut(s) 290
Psp6I CCWGG 6 cut(s) 43, 124, 216, 441, 655, 792
PspGI CCWGG 6 cut(s) 43, 124, 216, 441, 655, 792
PspN4I GGNNCC 5 cut(s) 215, 228, 291, 421, 736
PspPI GGNCC 3 cut(s) 47, 290, 735
PspPPI RGGWCCY 1 cut(s) 290
PsuI RGATCY 2 cut(s) 213, 361
PsyI GACNNNGTC 1 cut(s) 17
RsaI GTAC 2 cut(s) 120, 228
RsaNI GTAC 2 cut(s) 119, 227
RseI CAYNNNNRTG 3 cut(s) 103, 568, 578
SaqAI TTAA 1 cut(s) 180
SatI GCNGC 7 cut(s) 59, 92, 142, 154, 403, 418, 439
Sau3AI GATC 5 cut(s) 6, 67, 213, 253, 361
Sau96I GGNCC 3 cut(s) 47, 290, 735
ScrFI CCNGG 6 cut(s) 45, 126, 218, 443, 657, 794
SinI GGWCC 2 cut(s) 290, 735
SmiMI CAYNNNNRTG 3 cut(s) 103, 568, 578
Sse9I AATT 4 cut(s) 38, 530, 608, 843
SseBI AGGCCT 1 cut(s) 727
SsiI CCGC 6 cut(s) 58, 91, 402, 417, 438, 774
SspMI CTAG 1 cut(s) 822
StuI AGGCCT 1 cut(s) 727
StyD4I CCNGG 6 cut(s) 43, 124, 216, 441, 655, 792
StyI CCWWGG 3 cut(s) 136, 293, 423
TaaI ACNGT 2 cut(s) 358, 386
TaiI ACGT 1 cut(s) 328
TaqI TCGA 4 cut(s) 5, 12, 70, 371
TasI AATT 4 cut(s) 38, 530, 608, 843
TauI GCSGC 5 cut(s) 61, 94, 405, 420, 441
TfiI GAWTC 2 cut(s) 300, 748
Tru1I TTAA 1 cut(s) 180
Tru9I TTAA 1 cut(s) 180
TscAI CASTG 3 cut(s) 28, 354, 631
TseFI GTSAC 1 cut(s) 19
TseI GCWGC 2 cut(s) 141, 153
Tsp45I GTSAC 1 cut(s) 19
TspDTI ATGAA 2 cut(s) 183, 507
TspRI CASTG 3 cut(s) 28, 354, 631
Tth111I GACNNNGTC 1 cut(s) 17
VpaK11BI GGWCC 2 cut(s) 290, 735
XapI RAATTY 2 cut(s) 38, 608
XceI RCATGY 1 cut(s) 573
XmnI GAANNNNTTC 1 cut(s) 377
XspI CTAG 1 cut(s) 822
Zsp2I ATGCAT 1 cut(s) 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.