AT3G56830

Protein of unknown function (DUF565)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0008566
Physical Location & Seq
Reverse (-)
21042430 .. 21044503
2074 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G56830.3

Sequence Viewer

Length: 567 bp
ATGGGATTTGCCATCACGGTGACATTACCTAATTGTGGAAGTAAGTTCTTGCCTAAAAGGAGTTTCAGAGACAAAGGTGCTTTGTCTCTTGCCATTTGTTCCCTCTGTCATTTCCTTCATCCTGCACAGCCTCTGTTGGTCAGACATCAAAGGAGGATGTCATGGACAACTATCAAAAGCTCGGTTGGTGGGGACAGATTCGATCCAGCATCTGGTTCTTCAAGTAATAATAGCTCCAGAGGTCTACGGCTAATCAAAGCCATACAAGTTCTCAGAAGTAAGCTTCTGGTGAAAATTCAGGAGATAAAGAAAGATCTCCCCAAGAAACTGTTCTTTCTCTTGGTGGGATTCTACTCTGCAACTGCTTTCTCTACATTCATAGGACAAACAGGAGACTGGGATGTTCTATCTGCTGGATTGGCTGTGCTTGTAGTTGAATGCATTGGAGCTTTAATGTACAGAGCTTCTATTCCATTAATCAACAAGATGCGGAGCACGATCACCATGTTTAACTATTGGAAGACTGGACTCGCCCTGGGACTGTTCCTAGACTCATTCAAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF565

Protein Analysis

188

Amino Acids

20.84

Weight (kDa)

10.26

Isoelectric Point (pI)

40.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF565 PF04483 129 - 187 2.8e-17 Protein of unknown function (DUF565)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 212
AccI GTMKAC 1 cut(s) 244
AciI CCGC 1 cut(s) 490
AclWI GGATC 1 cut(s) 197
AcsI RAATTY 1 cut(s) 294
AfaI GTAC 1 cut(s) 458
AfiI CCNNNNNNNGG 2 cut(s) 35, 212
AgsI TTSAA 3 cut(s) 222, 437, 559
AjnI CCWGG 1 cut(s) 534
AjuI GAANNNNNNNTTGG 2 cut(s) 314, 346
AluBI AGCT 5 cut(s) 180, 234, 283, 449, 464
AluI AGCT 5 cut(s) 180, 234, 283, 449, 464
Alw21I GWGCWC 1 cut(s) 497
Alw26I GTCTC 3 cut(s) 63, 90, 387
AlwI GGATC 1 cut(s) 197
AlwNI CAGNNNCTG 2 cut(s) 133, 212
ApoI RAATTY 1 cut(s) 294
AseI ATTAAT 1 cut(s) 476
Asp700I GAANNNNTTC 1 cut(s) 329
AsuHPI GGTGA 3 cut(s) 31, 301, 493
BbsI GAAGAC 1 cut(s) 527
Bbv12I GWGCWC 1 cut(s) 497
BccI CCATC 1 cut(s) 20
BceAI ACGGC 1 cut(s) 263
BciT130I CCWGG 1 cut(s) 536
BcoDI GTCTC 3 cut(s) 63, 90, 387
BfaI CTAG 1 cut(s) 548
BglII AGATCT 1 cut(s) 313
Bme1390I CCNGG 1 cut(s) 536
BmrFI CCNGG 1 cut(s) 536
BmrI ACTGGG 1 cut(s) 406
BmsI GCATC 2 cut(s) 218, 477
BmuI ACTGGG 1 cut(s) 406
BpiI GAAGAC 1 cut(s) 527
BpmI CTGGAG 1 cut(s) 220
BsaJI CCNNGG 2 cut(s) 534, 535
Bsc4I CCNNNNNNNGG 2 cut(s) 35, 212
Bse1I ACTGG 2 cut(s) 401, 529
BseBI CCWGG 1 cut(s) 536
BseDI CCNNGG 2 cut(s) 534, 535
BseGI GGATG 3 cut(s) 118, 162, 406
BseLI CCNNNNNNNGG 2 cut(s) 35, 212
BseMII CTCAG 1 cut(s) 286
BseNI ACTGG 2 cut(s) 401, 529
BsgI GTGCAG 1 cut(s) 108
BsiHKAI GWGCWC 1 cut(s) 497
BslFI GGGAC 2 cut(s) 206, 552
BslI CCNNNNNNNGG 2 cut(s) 35, 212
BsmAI GTCTC 3 cut(s) 63, 90, 387
BsmFI GGGAC 2 cut(s) 206, 552
BsmI GAATGC 1 cut(s) 443
Bsp1286I GDGCHC 1 cut(s) 497
Bsp1407I TGTACA 1 cut(s) 456
Bsp143I GATC 3 cut(s) 202, 313, 498
BspACI CCGC 1 cut(s) 490
BspCNI CTCAG 1 cut(s) 285
BspPI GGATC 1 cut(s) 197
BsrGI TGTACA 1 cut(s) 456
BsrI ACTGG 2 cut(s) 401, 529
BssECI CCNNGG 2 cut(s) 534, 535
BssMI GATC 3 cut(s) 202, 313, 498
Bst2UI CCWGG 1 cut(s) 536
Bst4CI ACNGT 3 cut(s) 19, 330, 543
BstAUI TGTACA 1 cut(s) 456
BstDEI CTNAG 1 cut(s) 272
BstF5I GGATG 3 cut(s) 118, 162, 406
BstKTI GATC 3 cut(s) 205, 316, 501
BstMAI GTCTC 3 cut(s) 63, 90, 387
BstMBI GATC 3 cut(s) 202, 313, 498
BstMWI GCNNNNNNNGC 1 cut(s) 419
BstNI CCWGG 1 cut(s) 536
BstSCI CCNGG 1 cut(s) 534
BstV2I GAAGAC 1 cut(s) 527
BstX2I RGATCY 1 cut(s) 313
BstYI RGATCY 1 cut(s) 313
BtsCI GGATG 3 cut(s) 118, 162, 406
CaiI CAGNNNCTG 2 cut(s) 133, 212
Csp6I GTAC 1 cut(s) 457
CviAII CATG 2 cut(s) 162, 505
CviJI RGCY 9 cut(s) 130, 180, 234, 250, 260, 283, 422, 449, 464
CviKI_1 RGCY 9 cut(s) 130, 180, 234, 250, 260, 283, 422, 449, 464
CviQI GTAC 1 cut(s) 457
DdeI CTNAG 1 cut(s) 272
DpnI GATC 3 cut(s) 204, 315, 500
DpnII GATC 3 cut(s) 202, 313, 498
EcoRII CCWGG 1 cut(s) 534
EcoT22I ATGCAT 1 cut(s) 443
FaeI CATG 2 cut(s) 165, 508
FaiI YATR 4 cut(s) 163, 263, 380, 506
FaqI GGGAC 2 cut(s) 206, 552
FatI CATG 2 cut(s) 161, 504
FblI GTMKAC 1 cut(s) 244
FokI GGATG 3 cut(s) 105, 169, 413
FspBI CTAG 1 cut(s) 548
GsuI CTGGAG 1 cut(s) 220
Hin1II CATG 2 cut(s) 165, 508
HindIII AAGCTT 1 cut(s) 281
HinfI GANTC 4 cut(s) 198, 348, 528, 551
HphI GGTGA 3 cut(s) 31, 301, 493
Hpy166II GTNNAC 1 cut(s) 245
Hpy188I TCNGA 3 cut(s) 68, 143, 275
Hpy188III TCNNGA 2 cut(s) 237, 299
Hpy8I GTNNAC 1 cut(s) 245
HpyAV CCTTC 1 cut(s) 125
HpyCH4III ACNGT 3 cut(s) 19, 330, 543
HpyCH4V TGCA 3 cut(s) 125, 359, 441
HpyF10VI GCNNNNNNNGC 1 cut(s) 419
HpyF3I CTNAG 1 cut(s) 272
Hsp92II CATG 2 cut(s) 165, 508
Kzo9I GATC 3 cut(s) 202, 313, 498
LmnI GCTCC 3 cut(s) 239, 446, 492
LweI GCATC 2 cut(s) 218, 477
MaeI CTAG 1 cut(s) 548
MaeIII GTNAC 1 cut(s) 19
MalI GATC 3 cut(s) 204, 315, 500
MboI GATC 3 cut(s) 202, 313, 498
MboII GAAGA 2 cut(s) 210, 532
MflI RGATCY 1 cut(s) 313
MhlI GDGCHC 1 cut(s) 497
MluCI AATT 2 cut(s) 31, 294
MlyI GAGTC 2 cut(s) 522, 545
MnlI CCTC 4 cut(s) 113, 141, 147, 233
Mph1103I ATGCAT 1 cut(s) 443
MroXI GAANNNNTTC 1 cut(s) 329
MseI TTAA 3 cut(s) 452, 476, 510
MslI CAYNNNNRTG 1 cut(s) 17
MspR9I CCNGG 1 cut(s) 536
Mva1269I GAATGC 1 cut(s) 443
MvaI CCWGG 1 cut(s) 536
MwoI GCNNNNNNNGC 1 cut(s) 419
NdeII GATC 3 cut(s) 202, 313, 498
NlaIII CATG 2 cut(s) 165, 508
NmuCI GTSAC 1 cut(s) 19
NsiI ATGCAT 1 cut(s) 443
PasI CCCWGGG 1 cut(s) 535
PctI GAATGC 1 cut(s) 443
PdmI GAANNNNTTC 1 cut(s) 329
PfeI GAWTC 2 cut(s) 198, 348
PflMI CCANNNNNTGG 1 cut(s) 212
PleI GAGTC 2 cut(s) 522, 545
PpsI GAGTC 2 cut(s) 522, 545
PshBI ATTAAT 1 cut(s) 476
Psp6I CCWGG 1 cut(s) 534
PspGI CCWGG 1 cut(s) 534
PstNI CAGNNNCTG 2 cut(s) 133, 212
PsuI RGATCY 1 cut(s) 313
RsaI GTAC 1 cut(s) 458
RsaNI GTAC 1 cut(s) 457
RseI CAYNNNNRTG 1 cut(s) 17
SaqAI TTAA 3 cut(s) 452, 476, 510
Sau3AI GATC 3 cut(s) 202, 313, 498
SchI GAGTC 2 cut(s) 522, 545
ScrFI CCNGG 1 cut(s) 536
SduI GDGCHC 1 cut(s) 497
SetI ASST 8 cut(s) 31, 79, 182, 236, 244, 285, 451, 466
SfaNI GCATC 2 cut(s) 218, 477
SmiMI CAYNNNNRTG 1 cut(s) 17
Sse9I AATT 2 cut(s) 31, 294
SsiI CCGC 1 cut(s) 490
SspMI CTAG 1 cut(s) 548
StyD4I CCNGG 1 cut(s) 534
TaaI ACNGT 3 cut(s) 19, 330, 543
TaqI TCGA 1 cut(s) 201
TasI AATT 2 cut(s) 31, 294
TatI WGTACW 1 cut(s) 456
TfiI GAWTC 2 cut(s) 198, 348
Tru1I TTAA 3 cut(s) 452, 476, 510
Tru9I TTAA 3 cut(s) 452, 476, 510
TseFI GTSAC 1 cut(s) 19
Tsp45I GTSAC 1 cut(s) 19
TspDTI ATGAA 2 cut(s) 107, 367
Van91I CCANNNNNTGG 1 cut(s) 212
VspI ATTAAT 1 cut(s) 476
XapI RAATTY 1 cut(s) 294
XmiI GTMKAC 1 cut(s) 244
XmnI GAANNNNTTC 1 cut(s) 329
XspI CTAG 1 cut(s) 548
Zsp2I ATGCAT 1 cut(s) 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.