Rorug01G0205300

Ycf20-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0008566
Physical Location & Seq
Forward (+)
28773646 .. 28788443
14798 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0205300.1

Sequence Viewer

Length: 900 bp
ATGCCATCCCGGCTAATTAACTGGCAGGTGCAATTTTTTGGGAGGGAATGGGACTTCATGGATTTATACCATCTTACTGTCCTTCTGGCCATCCATTGCCTCTGTCTTCTAGCACCATTTCAGTTCACTTGGGGTGCACTTTGGGTGGCAATATCACTGTATTTTGTGTCGGGTATGGGTGTAACCATCTCTTACCATCGGAACCTTGCCCACCAGAGCTTTAAGGTCCCCAAATGGCTTGAATACTCTCTGGCTTATTGTGCAGTTTTTTCACTTCAGGGTAGTCCACTTGAATGGGTGAGCACCCATAGATACCACCATCAATTTACAGACAAATTGAGAGACACTCATAGCCCCACTAAGGGATTTTGGTTTAGTCACGTGAATTGGGTGTTCGATTATCATTCTCGGTTTGGAAGCTATGACGGACAACTGATGAAGAACGTGGGAGATTTGGAATGCCAACTATACTATAGGTTTCTTCATTATACATACTTCTTTCATTCATTTCTTCTTGGAGTTGCACTCTATGTGGCCGGAGGATTACCTTTTGTGGTTTGGGGAATGGGTGTGAGGTTGGCATTTCTTTTAGACGTTACTTTTTCAGTAAATTCCATTTGCCACACTAGGGGAAAACAAATATGGGATACTGGTGATGCCTCTAAAAACAACTGGTTGTTTGGATTGCTGGCACTAGGAGAAGGTTGGCACAATAATCACCATGCTTTTGAGTACTCAGCTCGACAGGGCTTGGAATGGTGGCAAATTGATGTTTCTTGGTATGTGATAAAGTTTTTTCAAGTTCTGAATCTGGCAACAGACGTGAAACTACCAACCGAGATCCAGAAGAGACAAAAAGCTCTAGCAGCAAAGTTGATCCTGGAGGATAAAGTAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF565

Protein Analysis

299

Amino Acids

35.03

Weight (kDa)

8.21

Isoelectric Point (pI)

28.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase PF00487 43 - 256 1.6e-15 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 16
Acc36I ACCTGC 1 cut(s) 16
AclWI GGATC 2 cut(s) 835, 871
AcoI YGGCCR 2 cut(s) 87, 534
AcsI RAATTY 1 cut(s) 610
AcuI CTGAAG 1 cut(s) 260
AcvI CACGTG 1 cut(s) 382
AfaI GTAC 1 cut(s) 734
AfiI CCNNNNNNNGG 3 cut(s) 361, 362, 628
AgsI TTSAA 3 cut(s) 242, 293, 800
AjiI CACGTC 1 cut(s) 823
AjnI CCWGG 1 cut(s) 879
AluBI AGCT 4 cut(s) 219, 420, 740, 860
AluI AGCT 4 cut(s) 219, 420, 740, 860
Alw21I GWGCWC 2 cut(s) 139, 305
Alw26I GTCTC 2 cut(s) 336, 844
Alw44I GTGCAC 1 cut(s) 135
AlwI GGATC 2 cut(s) 835, 871
AoxI GGCC 2 cut(s) 87, 534
ApaLI GTGCAC 1 cut(s) 135
ApeKI GCWGC 1 cut(s) 866
ApoI RAATTY 1 cut(s) 610
AspS9I GGNCC 1 cut(s) 226
AsuC2I CCSGG 1 cut(s) 10
AsuHPI GGTGA 3 cut(s) 310, 665, 710
AvaII GGWCC 1 cut(s) 226
BaeGI GKGCMC 1 cut(s) 139
BalI TGGCCA 1 cut(s) 89
BbrPI CACGTG 1 cut(s) 382
BbsI GAAGAC 1 cut(s) 98
Bbv12I GWGCWC 2 cut(s) 139, 305
BbvI GCAGC 1 cut(s) 878
BccI CCATC 6 cut(s) 13, 78, 98, 194, 204, 327
BciT130I CCWGG 1 cut(s) 881
BciVI GTATCC 1 cut(s) 640
BcnI CCSGG 1 cut(s) 10
BcoDI GTCTC 2 cut(s) 336, 844
BfaI CTAG 4 cut(s) 110, 627, 695, 863
BfmI CTRYAG 1 cut(s) 472
BfuAI ACCTGC 1 cut(s) 16
BfuI GTATCC 1 cut(s) 640
BglI GCCNNNNNGGC 1 cut(s) 10
BisI GCNGC 1 cut(s) 867
BlsI GCNGC 1 cut(s) 868
BmcAI AGTACT 1 cut(s) 734
Bme1390I CCNGG 2 cut(s) 10, 881
Bme18I GGWCC 1 cut(s) 226
BmgBI CACGTC 1 cut(s) 823
BmgT120I GGNCC 1 cut(s) 226
BmiI GGNNCC 2 cut(s) 203, 228
BmrFI CCNGG 2 cut(s) 10, 881
BmsI GCATC 1 cut(s) 646
BpiI GAAGAC 1 cut(s) 98
BplI GAGNNNNNCTC 4 cut(s) 331, 363, 510, 542
BpuMI CCSGG 1 cut(s) 10
BsaAI YACGTR 1 cut(s) 382
Bsc4I CCNNNNNNNGG 3 cut(s) 361, 362, 628
Bse1I ACTGG 3 cut(s) 26, 655, 677
Bse3DI GCAATG 1 cut(s) 94
BseBI CCWGG 1 cut(s) 881
BseGI GGATG 2 cut(s) 5, 90
BseLI CCNNNNNNNGG 3 cut(s) 361, 362, 628
BseMI GCAATG 1 cut(s) 94
BseMII CTCAG 1 cut(s) 750
BseNI ACTGG 3 cut(s) 26, 655, 677
BseSI GKGCMC 1 cut(s) 139
BseXI GCAGC 1 cut(s) 878
BsgI GTGCAG 1 cut(s) 282
BshFI GGCC 2 cut(s) 89, 536
BsiHKAI GWGCWC 2 cut(s) 139, 305
BsiSI CCGG 2 cut(s) 10, 537
BslFI GGGAC 2 cut(s) 65, 212
BslI CCNNNNNNNGG 3 cut(s) 361, 362, 628
BsmAI GTCTC 2 cut(s) 336, 844
BsmFI GGGAC 2 cut(s) 65, 212
BsmI GAATGC 1 cut(s) 464
BsnI GGCC 2 cut(s) 89, 536
Bsp1286I GDGCHC 2 cut(s) 139, 305
Bsp143I GATC 2 cut(s) 840, 876
BspANI GGCC 2 cut(s) 89, 536
BspCNI CTCAG 1 cut(s) 749
BspLI GGNNCC 2 cut(s) 203, 228
BspMI ACCTGC 1 cut(s) 16
BspPI GGATC 2 cut(s) 835, 871
BsrDI GCAATG 1 cut(s) 94
BsrI ACTGG 3 cut(s) 26, 655, 677
BssMI GATC 2 cut(s) 840, 876
Bst2UI CCWGG 1 cut(s) 881
Bst4CI ACNGT 2 cut(s) 79, 159
Bst6I CTCTTC 1 cut(s) 842
BstBAI YACGTR 1 cut(s) 382
BstC8I GCNNGC 1 cut(s) 690
BstDEI CTNAG 2 cut(s) 360, 736
BstF5I GGATG 2 cut(s) 5, 90
BstKTI GATC 2 cut(s) 843, 879
BstMAI GTCTC 2 cut(s) 336, 844
BstMBI GATC 2 cut(s) 840, 876
BstMWI GCNNNNNNNGC 3 cut(s) 10, 260, 866
BstNI CCWGG 1 cut(s) 881
BstSCI CCNGG 2 cut(s) 8, 879
BstSFI CTRYAG 1 cut(s) 472
BstSLI GKGCMC 1 cut(s) 139
BstV1I GCAGC 1 cut(s) 878
BstV2I GAAGAC 1 cut(s) 98
BstX2I RGATCY 1 cut(s) 840
BstXI CCANNNNNNTGG 1 cut(s) 294
BstYI RGATCY 1 cut(s) 840
BsuI GTATCC 1 cut(s) 640
BsuRI GGCC 2 cut(s) 89, 536
BtrI CACGTC 1 cut(s) 823
BtsCI GGATG 2 cut(s) 5, 90
BtsIMutI CAGTG 1 cut(s) 155
BveI ACCTGC 1 cut(s) 16
Cac8I GCNNGC 1 cut(s) 690
Cfr13I GGNCC 1 cut(s) 226
Csp6I GTAC 1 cut(s) 733
CviAII CATG 2 cut(s) 58, 722
CviQI GTAC 1 cut(s) 733
DdeI CTNAG 2 cut(s) 360, 736
DpnI GATC 2 cut(s) 842, 878
DpnII GATC 2 cut(s) 840, 876
EaeI YGGCCR 2 cut(s) 87, 534
Eam1104I CTCTTC 1 cut(s) 842
EarI CTCTTC 1 cut(s) 842
Eco47I GGWCC 1 cut(s) 226
Eco57I CTGAAG 1 cut(s) 260
Eco72I CACGTG 1 cut(s) 382
EcoO109I RGGNCCY 1 cut(s) 226
EcoRII CCWGG 1 cut(s) 879
FaeI CATG 2 cut(s) 61, 725
FaqI GGGAC 2 cut(s) 65, 212
FatI CATG 2 cut(s) 57, 721
Fnu4HI GCNGC 1 cut(s) 867
FokI GGATG 1 cut(s) 77
Fsp4HI GCNGC 1 cut(s) 867
FspBI CTAG 4 cut(s) 110, 627, 695, 863
GluI GCNGC 1 cut(s) 867
HaeIII GGCC 2 cut(s) 89, 536
HapII CCGG 2 cut(s) 10, 537
Hin1II CATG 2 cut(s) 61, 725
HinfI GANTC 1 cut(s) 808
HpaII CCGG 2 cut(s) 10, 537
HphI GGTGA 3 cut(s) 310, 665, 710
Hpy166II GTNNAC 3 cut(s) 126, 137, 287
Hpy188I TCNGA 2 cut(s) 201, 807
Hpy188III TCNNGA 1 cut(s) 844
Hpy8I GTNNAC 3 cut(s) 126, 137, 287
HpyAV CCTTC 2 cut(s) 92, 695
HpyCH4III ACNGT 2 cut(s) 79, 159
HpyCH4IV ACGT 4 cut(s) 381, 444, 594, 822
HpyCH4V TGCA 4 cut(s) 31, 137, 263, 524
HpyF10VI GCNNNNNNNGC 3 cut(s) 10, 260, 866
HpyF3I CTNAG 2 cut(s) 360, 736
HpySE526I ACGT 4 cut(s) 381, 444, 594, 822
Hsp92II CATG 2 cut(s) 61, 725
Kzo9I GATC 2 cut(s) 840, 876
Lsp1109I GCAGC 1 cut(s) 878
LweI GCATC 1 cut(s) 646
MaeI CTAG 4 cut(s) 110, 627, 695, 863
MaeII ACGT 4 cut(s) 381, 444, 594, 822
MaeIII GTNAC 3 cut(s) 181, 377, 595
MalI GATC 2 cut(s) 842, 878
MboI GATC 2 cut(s) 840, 876
MboII GAAGA 5 cut(s) 98, 451, 473, 503, 859
MflI RGATCY 1 cut(s) 840
MhlI GDGCHC 2 cut(s) 139, 305
MlsI TGGCCA 1 cut(s) 89
MluCI AATT 8 cut(s) 15, 32, 323, 335, 385, 610, 765, 894
MluNI TGGCCA 1 cut(s) 89
MnlI CCTC 6 cut(s) 36, 110, 533, 567, 670, 877
Mox20I TGGCCA 1 cut(s) 89
MscI TGGCCA 1 cut(s) 89
MseI TTAA 2 cut(s) 18, 222
MslI CAYNNNNRTG 1 cut(s) 292
Msp20I TGGCCA 1 cut(s) 89
MspI CCGG 2 cut(s) 10, 537
MspR9I CCNGG 2 cut(s) 10, 881
Mva1269I GAATGC 1 cut(s) 464
MvaI CCWGG 1 cut(s) 881
MwoI GCNNNNNNNGC 3 cut(s) 10, 260, 866
NciI CCSGG 1 cut(s) 10
NdeII GATC 2 cut(s) 840, 876
NlaIII CATG 2 cut(s) 61, 725
NlaIV GGNNCC 2 cut(s) 203, 228
NmuCI GTSAC 1 cut(s) 377
PaqCI CACCTGC 1 cut(s) 16
PctI GAATGC 1 cut(s) 464
PfeI GAWTC 1 cut(s) 808
PfoI TCCNGGA 1 cut(s) 879
PkrI GCNGC 1 cut(s) 868
PmaCI CACGTG 1 cut(s) 382
PmlI CACGTG 1 cut(s) 382
Ppu21I YACGTR 1 cut(s) 382
PpuMI RGGWCCY 1 cut(s) 226
Psp5II RGGWCCY 1 cut(s) 226
Psp6I CCWGG 1 cut(s) 879
PspCI CACGTG 1 cut(s) 382
PspGI CCWGG 1 cut(s) 879
PspN4I GGNNCC 2 cut(s) 203, 228
PspPI GGNCC 1 cut(s) 226
PspPPI RGGWCCY 1 cut(s) 226
PsuI RGATCY 1 cut(s) 840
RsaI GTAC 1 cut(s) 734
RsaNI GTAC 1 cut(s) 733
RseI CAYNNNNRTG 1 cut(s) 292
SaqAI TTAA 2 cut(s) 18, 222
SatI GCNGC 1 cut(s) 867
Sau3AI GATC 2 cut(s) 840, 876
Sau96I GGNCC 1 cut(s) 226
ScaI AGTACT 1 cut(s) 734
ScrFI CCNGG 2 cut(s) 10, 881
SduI GDGCHC 2 cut(s) 139, 305
SfaNI GCATC 1 cut(s) 646
SfcI CTRYAG 1 cut(s) 472
SinI GGWCC 1 cut(s) 226
SmiMI CAYNNNNRTG 1 cut(s) 292
Sse9I AATT 8 cut(s) 15, 32, 323, 335, 385, 610, 765, 894
SspMI CTAG 4 cut(s) 110, 627, 695, 863
StyD4I CCNGG 2 cut(s) 8, 879
TaaI ACNGT 2 cut(s) 79, 159
TaiI ACGT 4 cut(s) 384, 447, 597, 825
TaqI TCGA 2 cut(s) 396, 742
TasI AATT 8 cut(s) 15, 32, 323, 335, 385, 610, 765, 894
TatI WGTACW 1 cut(s) 732
TfiI GAWTC 1 cut(s) 808
Tru1I TTAA 2 cut(s) 18, 222
Tru9I TTAA 2 cut(s) 18, 222
TscAI CASTG 1 cut(s) 162
TseFI GTSAC 1 cut(s) 377
TseI GCWGC 1 cut(s) 866
Tsp45I GTSAC 1 cut(s) 377
TspDTI ATGAA 5 cut(s) 46, 452, 473, 491, 495
TspGWI ACGGA 1 cut(s) 441
TspRI CASTG 1 cut(s) 162
VneI GTGCAC 1 cut(s) 135
VpaK11BI GGWCC 1 cut(s) 226
XapI RAATTY 1 cut(s) 610
XspI CTAG 4 cut(s) 110, 627, 695, 863
ZrmI AGTACT 1 cut(s) 734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.