Rh1AG222700

Ycf20-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0008566
Physical Location & Seq
Forward (+)
42440347 .. 42442128
1782 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG222700.1

Sequence Viewer

Length: 759 bp
ATGGCACCACACCAGAAGTTTTACTCAGTACACCCAACAGAAAAGTCCAAAAAACTTATTTTGTGTGAGAGCCTCAAACTGTTGTATATGATAATGGCGACTGCAATTACAGTGCCTACCTATCCAAGAAATGTTAAGCTTTTATCTTCTAAGAGTGTTGGTTCCAGAGTTATAGCGCTGGGTCTTTGTGCAATGTCTTACAAACCCACTAGTACTTTTGGGCACAAGCTCAATTCTCGCTGCTGTTTCTTCTGTATGCCTGAACCTCTTCTAGTCAACAATTTCAAGAGGATTTCATGGTCTGTCAGAAGCAGTGTAGATAACAGTGGGTTAGACCCATCTCCAACAAATGGCACTACAGGCACAACCAGATTAATTAGGGTTATCCAAGCTATTCAATCCAAGTTAGGTGCCAGAATTGGAGAGATAAGGAGAGGTTTACCCGTGAAAATACTTTTCTTCCTAGTCGGTTTCTATTGTTCAACTGCTTATGCTACTGTTATTGGGCAAACGGGAGACTGGGACATTCTCTCTGCGGCCTTGGCTGTGCTTATTGTGGAGGGGATTGGGGCCCTCATGTACAAGGCTTCTCTTCCTTTATTGAAGAAGACTAAAAGCCTGATAACCAAGTTTAATTATTGGAAAGCTGGGCTTGCGATGGGTCTTTTCTTGGATTCGTTTAAATATGAAGTCAATGACATTTTCGGATTCAGTAACCCCTTCAGTTTTGTACTAGATGCATTTTCATTATTCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF565

Protein Analysis

252

Amino Acids

27.88

Weight (kDa)

9.74

Isoelectric Point (pI)

36.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF565 PF04483 170 - 228 6.8e-16 Protein of unknown function (DUF565)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 4, 410
AccB7I CCANNNNNTGG 1 cut(s) 350
AciI CCGC 1 cut(s) 536
AcuI CTGAAG 1 cut(s) 706
AfaI GTAC 4 cut(s) 30, 214, 581, 732
AfeI AGCGCT 1 cut(s) 177
AfiI CCNNNNNNNGG 1 cut(s) 350
AgsI TTSAA 4 cut(s) 286, 398, 483, 604
AhlI ACTAGT 1 cut(s) 209
AluBI AGCT 4 cut(s) 139, 229, 392, 647
AluI AGCT 4 cut(s) 139, 229, 392, 647
Alw26I GTCTC 1 cut(s) 510
Aor51HI AGCGCT 1 cut(s) 177
AoxI GGCC 2 cut(s) 537, 570
ApaI GGGCCC 1 cut(s) 574
ApeKI GCWGC 1 cut(s) 240
AseI ATTAAT 1 cut(s) 374
Asp700I GAANNNNTTC 1 cut(s) 267
AspLEI GCGC 1 cut(s) 178
AspS9I GGNCC 2 cut(s) 570, 571
BaeGI GKGCMC 2 cut(s) 225, 574
BanI GGYRCC 2 cut(s) 4, 410
BanII GRGCYC 1 cut(s) 574
BbsI GAAGAC 1 cut(s) 614
BbvI GCAGC 1 cut(s) 227
BccI CCATC 2 cut(s) 346, 652
BcoDI GTCTC 1 cut(s) 510
BcuI ACTAGT 1 cut(s) 209
BfaI CTAG 4 cut(s) 210, 272, 464, 734
BfmI CTRYAG 1 cut(s) 357
BfoI RGCGCY 1 cut(s) 179
BisI GCNGC 2 cut(s) 241, 537
BlsI GCNGC 2 cut(s) 242, 538
BmcAI AGTACT 1 cut(s) 214
BmgT120I GGNCC 2 cut(s) 570, 571
BmiI GGNNCC 5 cut(s) 6, 163, 412, 571, 572
BmrI ACTGGG 1 cut(s) 529
BmsI GCATC 1 cut(s) 727
BmuI ACTGGG 1 cut(s) 529
BpiI GAAGAC 1 cut(s) 614
BsaJI CCNNGG 1 cut(s) 540
Bsc4I CCNNNNNNNGG 1 cut(s) 350
Bse1I ACTGG 1 cut(s) 524
Bse3DI GCAATG 1 cut(s) 198
BseDI CCNNGG 1 cut(s) 540
BseLI CCNNNNNNNGG 1 cut(s) 350
BseMI GCAATG 1 cut(s) 198
BseMII CTCAG 1 cut(s) 39
BseNI ACTGG 1 cut(s) 524
BseSI GKGCMC 2 cut(s) 225, 574
BseXI GCAGC 1 cut(s) 227
BseYI CCCAGC 2 cut(s) 178, 647
BshFI GGCC 2 cut(s) 539, 572
BshNI GGYRCC 2 cut(s) 4, 410
BslFI GGGAC 1 cut(s) 536
BslI CCNNNNNNNGG 1 cut(s) 350
BsmAI GTCTC 1 cut(s) 510
BsmFI GGGAC 1 cut(s) 536
BsnI GGCC 2 cut(s) 539, 572
Bsp120I GGGCCC 1 cut(s) 570
Bsp1286I GDGCHC 2 cut(s) 225, 574
Bsp1407I TGTACA 1 cut(s) 579
BspACI CCGC 1 cut(s) 536
BspANI GGCC 2 cut(s) 539, 572
BspCNI CTCAG 1 cut(s) 38
BspLI GGNNCC 5 cut(s) 6, 163, 412, 571, 572
BspT107I GGYRCC 2 cut(s) 4, 410
BsrDI GCAATG 1 cut(s) 198
BsrGI TGTACA 1 cut(s) 579
BsrI ACTGG 1 cut(s) 524
BssECI CCNNGG 1 cut(s) 540
BssT1I CCWWGG 1 cut(s) 540
Bst4CI ACNGT 4 cut(s) 81, 112, 326, 499
Bst6I CTCTTC 2 cut(s) 273, 597
BstAUI TGTACA 1 cut(s) 579
BstC8I GCNNGC 1 cut(s) 654
BstDEI CTNAG 2 cut(s) 25, 150
BstH2I RGCGCY 1 cut(s) 179
BstHHI GCGC 1 cut(s) 178
BstMAI GTCTC 1 cut(s) 510
BstMWI GCNNNNNNNGC 3 cut(s) 360, 542, 653
BstSFI CTRYAG 1 cut(s) 357
BstSLI GKGCMC 2 cut(s) 225, 574
BstV1I GCAGC 1 cut(s) 227
BstV2I GAAGAC 1 cut(s) 614
BsuRI GGCC 2 cut(s) 539, 572
BtgZI GCGATG 1 cut(s) 671
BtsI GCAGTG 1 cut(s) 319
BtsIMutI CAGTG 3 cut(s) 117, 319, 331
Cac8I GCNNGC 1 cut(s) 654
CfoI GCGC 1 cut(s) 178
Cfr13I GGNCC 2 cut(s) 570, 571
Csp6I GTAC 4 cut(s) 29, 213, 580, 731
CviAII CATG 2 cut(s) 297, 577
CviQI GTAC 4 cut(s) 29, 213, 580, 731
DdeI CTNAG 2 cut(s) 25, 150
DraI TTTAAA 1 cut(s) 682
Eam1104I CTCTTC 2 cut(s) 273, 597
EarI CTCTTC 2 cut(s) 273, 597
Eco130I CCWWGG 1 cut(s) 540
Eco24I GRGCYC 1 cut(s) 574
Eco47III AGCGCT 1 cut(s) 177
Eco57I CTGAAG 1 cut(s) 706
EcoO109I RGGNCCY 2 cut(s) 570, 571
EcoT14I CCWWGG 1 cut(s) 540
EcoT22I ATGCAT 1 cut(s) 742
EcoT38I GRGCYC 1 cut(s) 574
ErhI CCWWGG 1 cut(s) 540
FaeI CATG 2 cut(s) 300, 580
FaiI YATR 8 cut(s) 87, 89, 173, 257, 298, 492, 578, 687
FalI AAGNNNNNCTT 2 cut(s) 636, 668
FaqI GGGAC 1 cut(s) 536
FatI CATG 2 cut(s) 296, 576
Fnu4HI GCNGC 2 cut(s) 241, 537
FriOI GRGCYC 1 cut(s) 574
Fsp4HI GCNGC 2 cut(s) 241, 537
FspBI CTAG 4 cut(s) 210, 272, 464, 734
GlaI GCGC 1 cut(s) 177
GluI GCNGC 2 cut(s) 241, 537
GsaI CCCAGC 2 cut(s) 182, 651
HaeII RGCGCY 1 cut(s) 179
HaeIII GGCC 2 cut(s) 539, 572
HhaI GCGC 1 cut(s) 178
Hin1II CATG 2 cut(s) 300, 580
Hin6I GCGC 1 cut(s) 176
HinP1I GCGC 1 cut(s) 176
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HindIII AAGCTT 1 cut(s) 137
HinfI GANTC 2 cut(s) 674, 708
Hpy166II GTNNAC 3 cut(s) 31, 277, 440
Hpy188I TCNGA 2 cut(s) 308, 707
Hpy188III TCNNGA 2 cut(s) 165, 286
Hpy8I GTNNAC 3 cut(s) 31, 277, 440
HpyAV CCTTC 1 cut(s) 730
HpyCH4III ACNGT 4 cut(s) 81, 112, 326, 499
HpyCH4V TGCA 3 cut(s) 104, 191, 740
HpyF10VI GCNNNNNNNGC 3 cut(s) 360, 542, 653
HpyF3I CTNAG 2 cut(s) 25, 150
Hsp92II CATG 2 cut(s) 300, 580
HspAI GCGC 1 cut(s) 176
Lsp1109I GCAGC 1 cut(s) 227
LweI GCATC 1 cut(s) 727
MaeI CTAG 4 cut(s) 210, 272, 464, 734
MaeIII GTNAC 1 cut(s) 713
MboII GAAGA 7 cut(s) 138, 241, 260, 451, 584, 616, 619
MhlI GDGCHC 2 cut(s) 225, 574
MluCI AATT 6 cut(s) 105, 232, 280, 375, 417, 634
MmeI TCCRAC 1 cut(s) 368
MnlI CCTC 6 cut(s) 83, 276, 282, 428, 553, 584
Mph1103I ATGCAT 1 cut(s) 742
MroXI GAANNNNTTC 1 cut(s) 267
MseI TTAA 5 cut(s) 135, 374, 633, 681, 757
MwoI GCNNNNNNNGC 3 cut(s) 360, 542, 653
NlaIII CATG 2 cut(s) 300, 580
NlaIV GGNNCC 5 cut(s) 6, 163, 412, 571, 572
NsiI ATGCAT 1 cut(s) 742
PdmI GAANNNNTTC 1 cut(s) 267
PfeI GAWTC 2 cut(s) 674, 708
PflMI CCANNNNNTGG 1 cut(s) 350
PkrI GCNGC 2 cut(s) 242, 538
PshBI ATTAAT 1 cut(s) 374
PspFI CCCAGC 2 cut(s) 178, 647
PspN4I GGNNCC 5 cut(s) 6, 163, 412, 571, 572
PspOMI GGGCCC 1 cut(s) 570
PspPI GGNCC 2 cut(s) 570, 571
RsaI GTAC 4 cut(s) 30, 214, 581, 732
RsaNI GTAC 4 cut(s) 29, 213, 580, 731
SaqAI TTAA 5 cut(s) 135, 374, 633, 681, 757
SatI GCNGC 2 cut(s) 241, 537
Sau96I GGNCC 2 cut(s) 570, 571
ScaI AGTACT 1 cut(s) 214
SduI GDGCHC 2 cut(s) 225, 574
SetI ASST 8 cut(s) 122, 141, 231, 268, 394, 412, 439, 649
SfaNI GCATC 1 cut(s) 727
SfcI CTRYAG 1 cut(s) 357
SpeI ACTAGT 1 cut(s) 209
Sse9I AATT 6 cut(s) 105, 232, 280, 375, 417, 634
SsiI CCGC 1 cut(s) 536
SspMI CTAG 4 cut(s) 210, 272, 464, 734
StyI CCWWGG 1 cut(s) 540
TaaI ACNGT 4 cut(s) 81, 112, 326, 499
TasI AATT 6 cut(s) 105, 232, 280, 375, 417, 634
TatI WGTACW 4 cut(s) 28, 212, 579, 730
TauI GCSGC 1 cut(s) 539
TfiI GAWTC 2 cut(s) 674, 708
Tru1I TTAA 5 cut(s) 135, 374, 633, 681, 757
Tru9I TTAA 5 cut(s) 135, 374, 633, 681, 757
TscAI CASTG 3 cut(s) 117, 319, 331
TseI GCWGC 1 cut(s) 240
TspDTI ATGAA 3 cut(s) 285, 702, 735
TspRI CASTG 3 cut(s) 117, 319, 331
Van91I CCANNNNNTGG 1 cut(s) 350
VspI ATTAAT 1 cut(s) 374
XmnI GAANNNNTTC 1 cut(s) 267
XspI CTAG 4 cut(s) 210, 272, 464, 734
ZrmI AGTACT 1 cut(s) 214
Zsp2I ATGCAT 1 cut(s) 742
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.