pycom07g10570

Ycf20-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0008566
Physical Location & Seq
Reverse (-)
10674392 .. 10675290
899 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g10570.1

Sequence Viewer

Length: 588 bp
ATGGTTTTGGATCTTTATGCAATGCTTCACAAACCTTCGCATGCTTTTGGCCAAAAACTCAGTTCTCGCTGCTGTTTACTCTGCAGACCTCAACCTCTACTGGTCAACAATTTCAAGAGGATGACATGGTCTATCAGAAGTAGTGTGGATAGCAGTGGGTCGGATCCATCTCCAACAAATGGCTCTACCGGCACGACACGATTAATTAGGGCAATCCAAGCTATTCAAACCAAGTTAGGTGTAAAAATTCGACAGCTACGGAGAGGTTTTCCAATGAAATTACTCTTCTTCTTAGTGGGTTTTTACTGTGCTACTGCTTATGCTACCGTTATTGGGCAAACAGGCGACTGGGACATTCTATCTGCCGCTTTTGCTGTGGTTATTGTGGAGGGGATTGGGGCACTCATGTATAAGGCGTCTATTCCTTTTGTAAAGAAGACTAGGAACCTGATAACCATGTTTAACTATTGGAAAGCTGGGCTTTCCATGGGTCTCTTCTTGGATTCATTCAAATATGAGTTCAATGACATCTTTGGATTCAGTAACCCCTTCAATTTCGAACTACACACTTTCTCAATATTCTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF565

Protein Analysis

196

Amino Acids

22.01

Weight (kDa)

9.79

Isoelectric Point (pI)

30.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF565 PF04483 113 - 171 7.2e-17 Protein of unknown function (DUF565)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 179
AciI CCGC 1 cut(s) 366
AclWI GGATC 3 cut(s) 18, 158, 171
AcoI YGGCCR 1 cut(s) 49
AcsI RAATTY 1 cut(s) 246
AcyI GRCGYC 1 cut(s) 416
AfiI CCNNNNNNNGG 2 cut(s) 179, 333
AgsI TTSAA 5 cut(s) 115, 227, 511, 523, 553
AluBI AGCT 3 cut(s) 221, 256, 476
AluI AGCT 3 cut(s) 221, 256, 476
Alw26I GTCTC 1 cut(s) 497
AlwI GGATC 3 cut(s) 18, 158, 171
AoxI GGCC 1 cut(s) 49
ApeKI GCWGC 1 cut(s) 69
ApoI RAATTY 1 cut(s) 246
AseI ATTAAT 1 cut(s) 203
AsuII TTCGAA 1 cut(s) 558
BaeGI GKGCMC 1 cut(s) 403
BalI TGGCCA 1 cut(s) 51
BamHI GGATCC 1 cut(s) 163
BbsI GAAGAC 1 cut(s) 443
BbvI GCAGC 1 cut(s) 56
BccI CCATC 1 cut(s) 175
BcoDI GTCTC 1 cut(s) 497
BfaI CTAG 1 cut(s) 441
BfmI CTRYAG 1 cut(s) 82
BisI GCNGC 2 cut(s) 70, 366
BlsI GCNGC 2 cut(s) 71, 367
BmiI GGNNCC 2 cut(s) 165, 446
BmrI ACTGGG 1 cut(s) 358
BmuI ACTGGG 1 cut(s) 358
BpiI GAAGAC 1 cut(s) 443
Bpu14I TTCGAA 1 cut(s) 558
BsaHI GRCGYC 1 cut(s) 416
BsaI GGTCTC 1 cut(s) 497
BsaJI CCNNGG 1 cut(s) 486
Bsc4I CCNNNNNNNGG 2 cut(s) 179, 333
Bse118I RCCGGY 1 cut(s) 188
Bse1I ACTGG 2 cut(s) 105, 353
Bse3DI GCAATG 1 cut(s) 27
BseDI CCNNGG 1 cut(s) 486
BseGI GGATG 1 cut(s) 126
BseLI CCNNNNNNNGG 2 cut(s) 179, 333
BseMI GCAATG 1 cut(s) 27
BseMII CTCAG 1 cut(s) 73
BseNI ACTGG 2 cut(s) 105, 353
BseSI GKGCMC 1 cut(s) 403
BseXI GCAGC 1 cut(s) 56
BseYI CCCAGC 1 cut(s) 476
BshFI GGCC 1 cut(s) 51
BsiSI CCGG 1 cut(s) 189
BslFI GGGAC 1 cut(s) 365
BslI CCNNNNNNNGG 2 cut(s) 179, 333
BsmAI GTCTC 1 cut(s) 497
BsmFI GGGAC 1 cut(s) 365
BsnI GGCC 1 cut(s) 51
Bso31I GGTCTC 1 cut(s) 497
Bsp119I TTCGAA 1 cut(s) 558
Bsp1286I GDGCHC 1 cut(s) 403
Bsp143I GATC 2 cut(s) 10, 163
Bsp19I CCATGG 1 cut(s) 486
BspACI CCGC 1 cut(s) 366
BspANI GGCC 1 cut(s) 51
BspCNI CTCAG 1 cut(s) 72
BspLI GGNNCC 2 cut(s) 165, 446
BspMAI CTGCAG 1 cut(s) 86
BspPI GGATC 3 cut(s) 18, 158, 171
BspT104I TTCGAA 1 cut(s) 558
BspTNI GGTCTC 1 cut(s) 497
BsrDI GCAATG 1 cut(s) 27
BsrFI RCCGGY 1 cut(s) 188
BsrI ACTGG 2 cut(s) 105, 353
BssAI RCCGGY 1 cut(s) 188
BssECI CCNNGG 1 cut(s) 486
BssMI GATC 2 cut(s) 10, 163
BssNI GRCGYC 1 cut(s) 416
BssT1I CCWWGG 1 cut(s) 486
Bst4CI ACNGT 2 cut(s) 308, 328
Bst6I CTCTTC 2 cut(s) 290, 500
BstACI GRCGYC 1 cut(s) 416
BstBI TTCGAA 1 cut(s) 558
BstC8I GCNNGC 1 cut(s) 42
BstDEI CTNAG 2 cut(s) 59, 292
BstDSI CCRYGG 1 cut(s) 486
BstF5I GGATG 1 cut(s) 126
BstKTI GATC 2 cut(s) 13, 166
BstMAI GTCTC 1 cut(s) 497
BstMBI GATC 2 cut(s) 10, 163
BstMWI GCNNNNNNNGC 3 cut(s) 189, 218, 371
BstNSI RCATGY 1 cut(s) 44
BstSFI CTRYAG 1 cut(s) 82
BstSLI GKGCMC 1 cut(s) 403
BstV1I GCAGC 1 cut(s) 56
BstV2I GAAGAC 1 cut(s) 443
BstX2I RGATCY 2 cut(s) 10, 163
BstYI RGATCY 2 cut(s) 10, 163
BsuRI GGCC 1 cut(s) 51
BtgI CCRYGG 1 cut(s) 486
BtsCI GGATG 1 cut(s) 126
BtsI GCAGTG 1 cut(s) 160
BtsIMutI CAGTG 1 cut(s) 160
Cac8I GCNNGC 1 cut(s) 42
Cfr10I RCCGGY 1 cut(s) 188
CseI GACGC 1 cut(s) 405
CviAII CATG 5 cut(s) 41, 126, 406, 457, 487
CviJI RGCY 6 cut(s) 51, 183, 221, 256, 476, 481
CviKI_1 RGCY 6 cut(s) 51, 183, 221, 256, 476, 481
DdeI CTNAG 2 cut(s) 59, 292
DpnI GATC 2 cut(s) 12, 165
DpnII GATC 2 cut(s) 10, 163
EaeI YGGCCR 1 cut(s) 49
Eam1104I CTCTTC 2 cut(s) 290, 500
EarI CTCTTC 2 cut(s) 290, 500
Eco130I CCWWGG 1 cut(s) 486
Eco31I GGTCTC 1 cut(s) 497
EcoT14I CCWWGG 1 cut(s) 486
ErhI CCWWGG 1 cut(s) 486
FaeI CATG 5 cut(s) 44, 129, 409, 460, 490
FaiI YATR 9 cut(s) 18, 42, 127, 321, 407, 411, 458, 488, 516
FalI AAGNNNNNCTT 2 cut(s) 465, 497
FaqI GGGAC 1 cut(s) 365
FatI CATG 5 cut(s) 40, 125, 405, 456, 486
Fnu4HI GCNGC 2 cut(s) 70, 366
FokI GGATG 1 cut(s) 133
Fsp4HI GCNGC 2 cut(s) 70, 366
FspBI CTAG 1 cut(s) 441
GluI GCNGC 2 cut(s) 70, 366
GsaI CCCAGC 1 cut(s) 480
HaeIII GGCC 1 cut(s) 51
HapII CCGG 1 cut(s) 189
HgaI GACGC 1 cut(s) 405
Hin1I GRCGYC 1 cut(s) 416
Hin1II CATG 5 cut(s) 44, 129, 409, 460, 490
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HinfI GANTC 2 cut(s) 503, 537
HpaII CCGG 1 cut(s) 189
Hpy166II GTNNAC 2 cut(s) 77, 106
Hpy188I TCNGA 2 cut(s) 137, 163
Hpy188III TCNNGA 1 cut(s) 115
Hpy8I GTNNAC 2 cut(s) 77, 106
HpyAV CCTTC 2 cut(s) 45, 559
HpyCH4III ACNGT 2 cut(s) 308, 328
HpyCH4V TGCA 2 cut(s) 20, 84
HpyF10VI GCNNNNNNNGC 3 cut(s) 189, 218, 371
HpyF3I CTNAG 2 cut(s) 59, 292
Hsp92I GRCGYC 1 cut(s) 416
Hsp92II CATG 5 cut(s) 44, 129, 409, 460, 490
Kzo9I GATC 2 cut(s) 10, 163
LpnPI CCDG 7 cut(s) 86, 202, 327, 334, 461, 462, 568
Lsp1109I GCAGC 1 cut(s) 56
MaeI CTAG 1 cut(s) 441
MaeIII GTNAC 1 cut(s) 542
MalI GATC 2 cut(s) 12, 165
MboI GATC 2 cut(s) 10, 163
MboII GAAGA 4 cut(s) 277, 280, 448, 487
MflI RGATCY 2 cut(s) 10, 163
MhlI GDGCHC 1 cut(s) 403
MlsI TGGCCA 1 cut(s) 51
MluCI AATT 5 cut(s) 109, 204, 246, 278, 553
MluNI TGGCCA 1 cut(s) 51
MmeI TCCRAC 2 cut(s) 141, 197
MnlI CCTC 5 cut(s) 99, 105, 111, 257, 382
Mox20I TGGCCA 1 cut(s) 51
MscI TGGCCA 1 cut(s) 51
MseI TTAA 2 cut(s) 203, 462
Msp20I TGGCCA 1 cut(s) 51
MspI CCGG 1 cut(s) 189
MwoI GCNNNNNNNGC 3 cut(s) 189, 218, 371
NcoI CCATGG 1 cut(s) 486
NdeII GATC 2 cut(s) 10, 163
NlaIII CATG 5 cut(s) 44, 129, 409, 460, 490
NlaIV GGNNCC 2 cut(s) 165, 446
NspI RCATGY 1 cut(s) 44
NspV TTCGAA 1 cut(s) 558
PaeI GCATGC 1 cut(s) 44
PfeI GAWTC 2 cut(s) 503, 537
PflFI GACNNNGTC 1 cut(s) 127
PflMI CCANNNNNTGG 1 cut(s) 179
PkrI GCNGC 2 cut(s) 71, 367
PshBI ATTAAT 1 cut(s) 203
PspFI CCCAGC 1 cut(s) 476
PspN4I GGNNCC 2 cut(s) 165, 446
PstI CTGCAG 1 cut(s) 86
PsuI RGATCY 2 cut(s) 10, 163
PsyI GACNNNGTC 1 cut(s) 127
SaqAI TTAA 2 cut(s) 203, 462
SatI GCNGC 2 cut(s) 70, 366
Sau3AI GATC 2 cut(s) 10, 163
SduI GDGCHC 1 cut(s) 403
SetI ASST 9 cut(s) 37, 91, 97, 223, 241, 258, 268, 450, 478
SfcI CTRYAG 1 cut(s) 82
SfuI TTCGAA 1 cut(s) 558
SphI GCATGC 1 cut(s) 44
Sse9I AATT 5 cut(s) 109, 204, 246, 278, 553
SsiI CCGC 1 cut(s) 366
SspI AATATT 1 cut(s) 579
SspMI CTAG 1 cut(s) 441
StyI CCWWGG 1 cut(s) 486
TaaI ACNGT 2 cut(s) 308, 328
TaqI TCGA 2 cut(s) 250, 558
TasI AATT 5 cut(s) 109, 204, 246, 278, 553
TauI GCSGC 1 cut(s) 368
TfiI GAWTC 2 cut(s) 503, 537
Tru1I TTAA 2 cut(s) 203, 462
Tru9I TTAA 2 cut(s) 203, 462
TscAI CASTG 1 cut(s) 160
TseI GCWGC 1 cut(s) 69
TspDTI ATGAA 2 cut(s) 290, 495
TspGWI ACGGA 1 cut(s) 274
TspRI CASTG 1 cut(s) 160
Tth111I GACNNNGTC 1 cut(s) 127
Van91I CCANNNNNTGG 1 cut(s) 179
VspI ATTAAT 1 cut(s) 203
XapI RAATTY 1 cut(s) 246
XceI RCATGY 1 cut(s) 44
XspI CTAG 1 cut(s) 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.