Rh1BG188600

Ycf20-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0008566
Physical Location & Seq
Forward (+)
30350820 .. 30357641
6822 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG188600.1

Sequence Viewer

Length: 672 bp
ATGATAATGGCGACTGCAATTACAGTGCCTACCTATCCAAGAAATGTTAAGCTTTTATCTTCTAAGTGTGTTGGTTCCAGAGTTATAGCGCTGGGTCTTTGTGCATTGTCTTACAAACCTACTAGTACTTTTGGGCACAAGCTCAATTCTCGCTGCTGTTTCTTCTGTATGCCTGAACCTCTTCTAGTCAACAATTTCAAGAGGATTTCATGGTCTGTCAGAAGCAGTGTAGATAACAGTGGGTTAGACCCATCTCCAACAAATGGCACTACAGGCACAGCAAGATTAATTAGGGTTATCCAAGCTATTCAATCCAAGTTAGGTGCCAGAATTGGAGAGATAAGGAGAGGTTTACCCGTGAAAATACTTTTCTTCCTAGTTGGTTTCTATTGTTCAACTGCTTATGCTACTGTTATTGGGCAAACGGGAGACTGGGACATTCTCTCTGCGGCCTTTGCTGTGCTTATTGTGGAGGGGATTGGGGCCCTCATGTACAAGGCTTCTCTTCCTTTATTGAAGAAGACTAAAAGCCTGATAACCAAGTTTAATTATTGGAAAGCTGGGCTTGCGATGGGTCTTTTCTTGGATTCGTTTAAATATGAAGTCAATGACATTTTCGGATTCAGCAACCCCTTCAGTTTTGTACTAGATGCATTTTCATTATTCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF565

Protein Analysis

223

Amino Acids

24.47

Weight (kDa)

9.7

Isoelectric Point (pI)

31.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF565 PF04483 141 - 199 3e-16 Protein of unknown function (DUF565)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 323
AccB7I CCANNNNNTGG 1 cut(s) 263
AciI CCGC 1 cut(s) 449
AcuI CTGAAG 1 cut(s) 619
AfaI GTAC 3 cut(s) 127, 494, 645
AfeI AGCGCT 1 cut(s) 90
AfiI CCNNNNNNNGG 1 cut(s) 263
AgsI TTSAA 4 cut(s) 199, 311, 396, 517
AhlI ACTAGT 1 cut(s) 122
AluBI AGCT 4 cut(s) 52, 142, 305, 560
AluI AGCT 4 cut(s) 52, 142, 305, 560
Alw26I GTCTC 1 cut(s) 423
Aor51HI AGCGCT 1 cut(s) 90
AoxI GGCC 2 cut(s) 450, 483
ApaI GGGCCC 1 cut(s) 487
ApeKI GCWGC 1 cut(s) 153
AseI ATTAAT 1 cut(s) 287
Asp700I GAANNNNTTC 1 cut(s) 180
AspLEI GCGC 1 cut(s) 91
AspS9I GGNCC 2 cut(s) 483, 484
BaeGI GKGCMC 2 cut(s) 138, 487
BanI GGYRCC 1 cut(s) 323
BanII GRGCYC 1 cut(s) 487
BbsI GAAGAC 1 cut(s) 527
BbvI GCAGC 1 cut(s) 140
BccI CCATC 2 cut(s) 259, 565
BcoDI GTCTC 1 cut(s) 423
BcuI ACTAGT 1 cut(s) 122
BfaI CTAG 4 cut(s) 123, 185, 377, 647
BfmI CTRYAG 1 cut(s) 270
BfoI RGCGCY 1 cut(s) 92
BisI GCNGC 2 cut(s) 154, 450
BlsI GCNGC 2 cut(s) 155, 451
BmcAI AGTACT 1 cut(s) 127
BmgT120I GGNCC 2 cut(s) 483, 484
BmiI GGNNCC 4 cut(s) 76, 325, 484, 485
BmrI ACTGGG 1 cut(s) 442
BmsI GCATC 1 cut(s) 640
BmuI ACTGGG 1 cut(s) 442
BpiI GAAGAC 1 cut(s) 527
Bsc4I CCNNNNNNNGG 1 cut(s) 263
Bse1I ACTGG 1 cut(s) 437
BseLI CCNNNNNNNGG 1 cut(s) 263
BseNI ACTGG 1 cut(s) 437
BseSI GKGCMC 2 cut(s) 138, 487
BseXI GCAGC 1 cut(s) 140
BseYI CCCAGC 2 cut(s) 91, 560
BshFI GGCC 2 cut(s) 452, 485
BshNI GGYRCC 1 cut(s) 323
BslFI GGGAC 1 cut(s) 449
BslI CCNNNNNNNGG 1 cut(s) 263
BsmAI GTCTC 1 cut(s) 423
BsmFI GGGAC 1 cut(s) 449
BsnI GGCC 2 cut(s) 452, 485
Bsp120I GGGCCC 1 cut(s) 483
Bsp1286I GDGCHC 2 cut(s) 138, 487
Bsp1407I TGTACA 1 cut(s) 492
BspACI CCGC 1 cut(s) 449
BspANI GGCC 2 cut(s) 452, 485
BspLI GGNNCC 4 cut(s) 76, 325, 484, 485
BspT107I GGYRCC 1 cut(s) 323
BsrGI TGTACA 1 cut(s) 492
BsrI ACTGG 1 cut(s) 437
Bst4CI ACNGT 3 cut(s) 25, 239, 412
Bst6I CTCTTC 2 cut(s) 186, 510
BstAUI TGTACA 1 cut(s) 492
BstC8I GCNNGC 1 cut(s) 567
BstDEI CTNAG 1 cut(s) 63
BstH2I RGCGCY 1 cut(s) 92
BstHHI GCGC 1 cut(s) 91
BstMAI GTCTC 1 cut(s) 423
BstMWI GCNNNNNNNGC 3 cut(s) 273, 455, 566
BstSFI CTRYAG 1 cut(s) 270
BstSLI GKGCMC 2 cut(s) 138, 487
BstV1I GCAGC 1 cut(s) 140
BstV2I GAAGAC 1 cut(s) 527
BsuRI GGCC 2 cut(s) 452, 485
BtgZI GCGATG 1 cut(s) 584
BtsI GCAGTG 1 cut(s) 232
BtsIMutI CAGTG 3 cut(s) 30, 232, 244
Cac8I GCNNGC 1 cut(s) 567
CfoI GCGC 1 cut(s) 91
Cfr13I GGNCC 2 cut(s) 483, 484
Csp6I GTAC 3 cut(s) 126, 493, 644
CviAII CATG 2 cut(s) 210, 490
CviJI RGCY 9 cut(s) 52, 142, 305, 452, 485, 500, 531, 560, 565
CviKI_1 RGCY 9 cut(s) 52, 142, 305, 452, 485, 500, 531, 560, 565
CviQI GTAC 3 cut(s) 126, 493, 644
DdeI CTNAG 1 cut(s) 63
DraI TTTAAA 1 cut(s) 595
Eam1104I CTCTTC 2 cut(s) 186, 510
EarI CTCTTC 2 cut(s) 186, 510
Eco24I GRGCYC 1 cut(s) 487
Eco47III AGCGCT 1 cut(s) 90
Eco57I CTGAAG 1 cut(s) 619
EcoO109I RGGNCCY 2 cut(s) 483, 484
EcoT22I ATGCAT 1 cut(s) 655
EcoT38I GRGCYC 1 cut(s) 487
FaeI CATG 2 cut(s) 213, 493
FaiI YATR 6 cut(s) 86, 170, 211, 405, 491, 600
FalI AAGNNNNNCTT 2 cut(s) 549, 581
FaqI GGGAC 1 cut(s) 449
FatI CATG 2 cut(s) 209, 489
Fnu4HI GCNGC 2 cut(s) 154, 450
FriOI GRGCYC 1 cut(s) 487
Fsp4HI GCNGC 2 cut(s) 154, 450
FspBI CTAG 4 cut(s) 123, 185, 377, 647
GlaI GCGC 1 cut(s) 90
GluI GCNGC 2 cut(s) 154, 450
GsaI CCCAGC 2 cut(s) 95, 564
HaeII RGCGCY 1 cut(s) 92
HaeIII GGCC 2 cut(s) 452, 485
HhaI GCGC 1 cut(s) 91
Hin1II CATG 2 cut(s) 213, 493
Hin6I GCGC 1 cut(s) 89
HinP1I GCGC 1 cut(s) 89
HincII GTYRAC 1 cut(s) 190
HindII GTYRAC 1 cut(s) 190
HindIII AAGCTT 1 cut(s) 50
HinfI GANTC 2 cut(s) 587, 621
Hpy166II GTNNAC 2 cut(s) 190, 353
Hpy188I TCNGA 2 cut(s) 221, 620
Hpy188III TCNNGA 2 cut(s) 78, 199
Hpy8I GTNNAC 2 cut(s) 190, 353
HpyAV CCTTC 1 cut(s) 643
HpyCH4III ACNGT 3 cut(s) 25, 239, 412
HpyCH4V TGCA 3 cut(s) 17, 104, 653
HpyF10VI GCNNNNNNNGC 3 cut(s) 273, 455, 566
HpyF3I CTNAG 1 cut(s) 63
Hsp92II CATG 2 cut(s) 213, 493
HspAI GCGC 1 cut(s) 89
LpnPI CCDG 8 cut(s) 77, 91, 186, 258, 340, 418, 545, 546
Lsp1109I GCAGC 1 cut(s) 140
LweI GCATC 1 cut(s) 640
MaeI CTAG 4 cut(s) 123, 185, 377, 647
MboII GAAGA 7 cut(s) 51, 154, 173, 364, 497, 529, 532
MhlI GDGCHC 2 cut(s) 138, 487
MluCI AATT 6 cut(s) 18, 145, 193, 288, 330, 547
MmeI TCCRAC 1 cut(s) 281
MnlI CCTC 5 cut(s) 189, 195, 341, 466, 497
Mph1103I ATGCAT 1 cut(s) 655
MroXI GAANNNNTTC 1 cut(s) 180
MseI TTAA 5 cut(s) 48, 287, 546, 594, 670
MwoI GCNNNNNNNGC 3 cut(s) 273, 455, 566
NlaIII CATG 2 cut(s) 213, 493
NlaIV GGNNCC 4 cut(s) 76, 325, 484, 485
NsiI ATGCAT 1 cut(s) 655
PdmI GAANNNNTTC 1 cut(s) 180
PfeI GAWTC 2 cut(s) 587, 621
PflMI CCANNNNNTGG 1 cut(s) 263
PkrI GCNGC 2 cut(s) 155, 451
PshBI ATTAAT 1 cut(s) 287
PspFI CCCAGC 2 cut(s) 91, 560
PspN4I GGNNCC 4 cut(s) 76, 325, 484, 485
PspOMI GGGCCC 1 cut(s) 483
PspPI GGNCC 2 cut(s) 483, 484
RsaI GTAC 3 cut(s) 127, 494, 645
RsaNI GTAC 3 cut(s) 126, 493, 644
SaqAI TTAA 5 cut(s) 48, 287, 546, 594, 670
SatI GCNGC 2 cut(s) 154, 450
Sau96I GGNCC 2 cut(s) 483, 484
ScaI AGTACT 1 cut(s) 127
SduI GDGCHC 2 cut(s) 138, 487
SetI ASST 9 cut(s) 35, 54, 121, 144, 181, 307, 325, 352, 562
SfaNI GCATC 1 cut(s) 640
SfcI CTRYAG 1 cut(s) 270
SpeI ACTAGT 1 cut(s) 122
Sse9I AATT 6 cut(s) 18, 145, 193, 288, 330, 547
SsiI CCGC 1 cut(s) 449
SspMI CTAG 4 cut(s) 123, 185, 377, 647
TaaI ACNGT 3 cut(s) 25, 239, 412
TasI AATT 6 cut(s) 18, 145, 193, 288, 330, 547
TatI WGTACW 3 cut(s) 125, 492, 643
TauI GCSGC 1 cut(s) 452
TfiI GAWTC 2 cut(s) 587, 621
Tru1I TTAA 5 cut(s) 48, 287, 546, 594, 670
Tru9I TTAA 5 cut(s) 48, 287, 546, 594, 670
TscAI CASTG 3 cut(s) 30, 232, 244
TseI GCWGC 1 cut(s) 153
TspDTI ATGAA 3 cut(s) 198, 615, 648
TspRI CASTG 3 cut(s) 30, 232, 244
Van91I CCANNNNNTGG 1 cut(s) 263
VspI ATTAAT 1 cut(s) 287
XmnI GAANNNNTTC 1 cut(s) 180
XspI CTAG 4 cut(s) 123, 185, 377, 647
ZrmI AGTACT 1 cut(s) 127
Zsp2I ATGCAT 1 cut(s) 655
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.