FvH4_7g11410

Ycf20-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0008566
Physical Location & Seq
Forward (+)
10589920 .. 10592504
2585 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g11410.t1

Sequence Viewer

Length: 672 bp
ATGATAATGGCGGCTGCAATTACATTGCCTACCTATCCAAGGAGTGTTAAGCTTTTATCTTCTGAGTGTATCACTTTCAAAGTTATGGCGCTGGGTCTTTATGCAATGTCTTACAAACCTACTGGTGCTTTTGGCCGCAAGCTCAATTCTCACGGCTGTTTATTCTGCGTGCCTGAGCCTCTTTTGGTCAACAATTTCAAGAGGATTTCATGGTCTATCAGAAGCAGTGTAGATAACAATGGGTTAGACCCATCTCCAACAAATGGCAGCACAGGCACAACACGATTAATTAGGGTTATCCAAGATATTCAAGCCAAGTTAGGTGCCAGAATTGGAGAGATAAGGAGAGGTTTACCCATGAAAATATTTTTCTTCCTAGTTGGTTTCTATTGTTCGACTGCTTATTCTACTGTTATTGGGCAAACGGGAGACTGGGACATTCTCTCTGCTGCCTTTGCTATGCTTATTGTGGAGGGGATTGGGGCACTCATGTACAAGGCTTCTCTTCCTTTATTGGAGAAGACTAAAAGCCTGATAACCAAGTTTAATTATTGGAAAGCTGGGCTTGTGATGGGTCTCTTCTTGGATTCATTTAAATATGAAATCGATGACATTTTCGGATTCAGTAACCCCTTCAGCTTTGTACTAGATGCACTCTCAGTATTCCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF565

Protein Analysis

224

Amino Acids

24.64

Weight (kDa)

9.28

Isoelectric Point (pI)

29.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF565 PF04483 141 - 199 7.4e-16 Protein of unknown function (DUF565)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 323
AccB7I CCANNNNNTGG 1 cut(s) 263
AciI CCGC 2 cut(s) 11, 136
AcoI YGGCCR 1 cut(s) 133
AcuI CTGAAG 1 cut(s) 619
AfaI GTAC 2 cut(s) 494, 645
AfiI CCNNNNNNNGG 2 cut(s) 39, 263
AgsI TTSAA 3 cut(s) 79, 199, 311
AluBI AGCT 4 cut(s) 52, 142, 560, 639
AluI AGCT 4 cut(s) 52, 142, 560, 639
Alw26I GTCTC 2 cut(s) 423, 581
AoxI GGCC 1 cut(s) 133
ApeKI GCWGC 3 cut(s) 14, 267, 449
AseI ATTAAT 1 cut(s) 287
AspLEI GCGC 1 cut(s) 91
BaeGI GKGCMC 1 cut(s) 487
BanI GGYRCC 1 cut(s) 323
BbsI GAAGAC 1 cut(s) 527
BbvI GCAGC 2 cut(s) 279, 436
BccI CCATC 2 cut(s) 259, 565
BceAI ACGGC 1 cut(s) 169
BcoDI GTCTC 2 cut(s) 423, 581
BfaI CTAG 2 cut(s) 377, 647
BfoI RGCGCY 1 cut(s) 92
BisI GCNGC 5 cut(s) 12, 15, 136, 268, 450
BlsI GCNGC 5 cut(s) 13, 16, 137, 269, 451
BmiI GGNNCC 1 cut(s) 325
BmrI ACTGGG 1 cut(s) 442
BmsI GCATC 1 cut(s) 640
BmuI ACTGGG 1 cut(s) 442
BpiI GAAGAC 1 cut(s) 527
Bpu10I CCTNAGC 1 cut(s) 174
Bsa29I ATCGAT 1 cut(s) 606
BsaI GGTCTC 1 cut(s) 581
BsaJI CCNNGG 1 cut(s) 38
Bsc4I CCNNNNNNNGG 2 cut(s) 39, 263
Bse1I ACTGG 2 cut(s) 127, 437
Bse3DI GCAATG 2 cut(s) 23, 111
BseCI ATCGAT 1 cut(s) 606
BseDI CCNNGG 1 cut(s) 38
BseLI CCNNNNNNNGG 2 cut(s) 39, 263
BseMI GCAATG 2 cut(s) 23, 111
BseMII CTCAG 3 cut(s) 54, 165, 672
BseNI ACTGG 2 cut(s) 127, 437
BseSI GKGCMC 1 cut(s) 487
BseXI GCAGC 2 cut(s) 279, 436
BseYI CCCAGC 2 cut(s) 91, 560
BshFI GGCC 1 cut(s) 135
BshNI GGYRCC 1 cut(s) 323
BshVI ATCGAT 1 cut(s) 606
BslFI GGGAC 1 cut(s) 449
BslI CCNNNNNNNGG 2 cut(s) 39, 263
BsmAI GTCTC 2 cut(s) 423, 581
BsmFI GGGAC 1 cut(s) 449
BsnI GGCC 1 cut(s) 135
Bso31I GGTCTC 1 cut(s) 581
Bsp1286I GDGCHC 1 cut(s) 487
Bsp1407I TGTACA 1 cut(s) 492
BspACI CCGC 2 cut(s) 11, 136
BspANI GGCC 1 cut(s) 135
BspCNI CTCAG 3 cut(s) 55, 166, 671
BspDI ATCGAT 1 cut(s) 606
BspLI GGNNCC 1 cut(s) 325
BspT107I GGYRCC 1 cut(s) 323
BspTNI GGTCTC 1 cut(s) 581
BsrDI GCAATG 2 cut(s) 23, 111
BsrGI TGTACA 1 cut(s) 492
BsrI ACTGG 2 cut(s) 127, 437
BssECI CCNNGG 1 cut(s) 38
BssT1I CCWWGG 1 cut(s) 38
Bst4CI ACNGT 1 cut(s) 412
Bst6I CTCTTC 2 cut(s) 510, 584
BstAUI TGTACA 1 cut(s) 492
BstC8I GCNNGC 2 cut(s) 140, 170
BstDEI CTNAG 3 cut(s) 63, 174, 658
BstENI CCTNNNNNAGG 1 cut(s) 37
BstH2I RGCGCY 1 cut(s) 92
BstHHI GCGC 1 cut(s) 91
BstMAI GTCTC 2 cut(s) 423, 581
BstMWI GCNNNNNNNGC 2 cut(s) 273, 455
BstSLI GKGCMC 1 cut(s) 487
BstV1I GCAGC 2 cut(s) 279, 436
BstV2I GAAGAC 1 cut(s) 527
Bsu15I ATCGAT 1 cut(s) 606
BsuRI GGCC 1 cut(s) 135
BsuTUI ATCGAT 1 cut(s) 606
BtsI GCAGTG 1 cut(s) 232
BtsIMutI CAGTG 1 cut(s) 232
Cac8I GCNNGC 2 cut(s) 140, 170
CfoI GCGC 1 cut(s) 91
ClaI ATCGAT 1 cut(s) 606
Csp6I GTAC 2 cut(s) 493, 644
CviAII CATG 3 cut(s) 210, 358, 490
CviQI GTAC 2 cut(s) 493, 644
DdeI CTNAG 3 cut(s) 63, 174, 658
DraI TTTAAA 1 cut(s) 595
EaeI YGGCCR 1 cut(s) 133
Eam1104I CTCTTC 2 cut(s) 510, 584
EarI CTCTTC 2 cut(s) 510, 584
Eco130I CCWWGG 1 cut(s) 38
Eco31I GGTCTC 1 cut(s) 581
Eco57I CTGAAG 1 cut(s) 619
EcoNI CCTNNNNNAGG 1 cut(s) 37
EcoT14I CCWWGG 1 cut(s) 38
ErhI CCWWGG 1 cut(s) 38
FaeI CATG 3 cut(s) 213, 361, 493
FaiI YATR 7 cut(s) 86, 102, 211, 359, 461, 491, 600
FalI AAGNNNNNCTT 2 cut(s) 549, 581
FaqI GGGAC 1 cut(s) 449
FatI CATG 3 cut(s) 209, 357, 489
Fnu4HI GCNGC 5 cut(s) 12, 15, 136, 268, 450
Fsp4HI GCNGC 5 cut(s) 12, 15, 136, 268, 450
FspBI CTAG 2 cut(s) 377, 647
GlaI GCGC 1 cut(s) 90
GluI GCNGC 5 cut(s) 12, 15, 136, 268, 450
GsaI CCCAGC 2 cut(s) 95, 564
HaeII RGCGCY 1 cut(s) 92
HaeIII GGCC 1 cut(s) 135
HhaI GCGC 1 cut(s) 91
Hin1II CATG 3 cut(s) 213, 361, 493
Hin6I GCGC 1 cut(s) 89
HinP1I GCGC 1 cut(s) 89
HincII GTYRAC 1 cut(s) 190
HindII GTYRAC 1 cut(s) 190
HindIII AAGCTT 1 cut(s) 50
HinfI GANTC 2 cut(s) 587, 621
Hpy166II GTNNAC 2 cut(s) 190, 353
Hpy188I TCNGA 3 cut(s) 64, 221, 620
Hpy188III TCNNGA 1 cut(s) 199
Hpy8I GTNNAC 2 cut(s) 190, 353
HpyAV CCTTC 1 cut(s) 643
HpyCH4III ACNGT 1 cut(s) 412
HpyCH4V TGCA 3 cut(s) 17, 104, 653
HpyF10VI GCNNNNNNNGC 2 cut(s) 273, 455
HpyF3I CTNAG 3 cut(s) 63, 174, 658
Hsp92II CATG 3 cut(s) 213, 361, 493
HspAI GCGC 1 cut(s) 89
LpnPI CCDG 8 cut(s) 77, 108, 186, 258, 340, 418, 545, 546
Lsp1109I GCAGC 2 cut(s) 279, 436
LweI GCATC 1 cut(s) 640
MaeI CTAG 2 cut(s) 377, 647
MaeIII GTNAC 1 cut(s) 626
MboII GAAGA 5 cut(s) 51, 364, 497, 532, 571
MhlI GDGCHC 1 cut(s) 487
MluCI AATT 6 cut(s) 18, 145, 193, 288, 330, 547
MmeI TCCRAC 1 cut(s) 281
MnlI CCTC 4 cut(s) 189, 195, 341, 466
MseI TTAA 4 cut(s) 48, 287, 546, 594
MwoI GCNNNNNNNGC 2 cut(s) 273, 455
NlaIII CATG 3 cut(s) 213, 361, 493
NlaIV GGNNCC 1 cut(s) 325
PfeI GAWTC 2 cut(s) 587, 621
PflMI CCANNNNNTGG 1 cut(s) 263
PkrI GCNGC 5 cut(s) 13, 16, 137, 269, 451
PshBI ATTAAT 1 cut(s) 287
PspFI CCCAGC 2 cut(s) 91, 560
PspN4I GGNNCC 1 cut(s) 325
RsaI GTAC 2 cut(s) 494, 645
RsaNI GTAC 2 cut(s) 493, 644
SaqAI TTAA 4 cut(s) 48, 287, 546, 594
SatI GCNGC 5 cut(s) 12, 15, 136, 268, 450
SduI GDGCHC 1 cut(s) 487
SetI ASST 8 cut(s) 35, 54, 121, 144, 325, 352, 562, 641
SfaNI GCATC 1 cut(s) 640
SmiI ATTTAAAT 1 cut(s) 595
Sse9I AATT 6 cut(s) 18, 145, 193, 288, 330, 547
SsiI CCGC 2 cut(s) 11, 136
SspI AATATT 1 cut(s) 366
SspMI CTAG 2 cut(s) 377, 647
StyI CCWWGG 1 cut(s) 38
SwaI ATTTAAAT 1 cut(s) 595
TaaI ACNGT 1 cut(s) 412
TaqI TCGA 2 cut(s) 395, 606
TasI AATT 6 cut(s) 18, 145, 193, 288, 330, 547
TatI WGTACW 2 cut(s) 492, 643
TauI GCSGC 2 cut(s) 14, 138
TfiI GAWTC 2 cut(s) 587, 621
Tru1I TTAA 4 cut(s) 48, 287, 546, 594
Tru9I TTAA 4 cut(s) 48, 287, 546, 594
TscAI CASTG 1 cut(s) 232
TseI GCWGC 3 cut(s) 14, 267, 449
TspDTI ATGAA 4 cut(s) 198, 374, 579, 615
TspRI CASTG 1 cut(s) 232
Van91I CCANNNNNTGG 1 cut(s) 263
VspI ATTAAT 1 cut(s) 287
XagI CCTNNNNNAGG 1 cut(s) 37
XspI CTAG 2 cut(s) 377, 647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.