Prupe.2G142000_v2.0.a1

Ycf20-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0008566
Physical Location & Seq
Forward (+)
19795265 .. 19797683
2419 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G142000.1

Sequence Viewer

Length: 672 bp
ATGATAATGGCGGCTGCAGTTACATCTGGTAACTATCCAAAAAGTGTTAAGCTTCATTCTTCCAAGTTTGTCAGGTCCAGAGTTATAGCTTTGGGTCTTTATCCCATGTTTTACAAACCTCCTCGTGCTTTTCGCCTGAAACTCAGTTCTAGCAGCTGTTTCTTCTGCATACCTCAGCCTCTACTAGTGAACAATTTCAAGAGGATGACATGGTCTATCAGAAGCAGTGTGGACAGCAGCGGGTTGGACCCATCTCCAACAAATGGCACTACTGGCACAACACGTTTAATTAGGGCTATCCAAGCTATTCAAACCAAGTTAGGTGGCAAAATTCGAGAGCTAAGGAGAGGTTTCCTATTTAAAGTACTCTTCTTCTTAGTGGGTTTCTACTGTGCAACTGCTTATGCTACTGTGATTGGGCAAACAGGCGACTGGGACATTCTATCTGCTGCCTTTGCTGTGGTTGTTGTGGAGGGGATTGGAGCCCTCATGTACAAGGCTTCTCTTCCTTTACTAATGAAGACTAGGAGCCTGATAACCATGTTTAATTATTGGAAAGCTGGGCTATCCATGGGTCTCTTCTTGGATTCATTTAAATATGAGTTCAATGATATTTTTGGATTCAGTAGCCCCTTCAATTTTGAACTTGATGCATTTTCAATGTTCTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF565

Protein Analysis

224

Amino Acids

24.92

Weight (kDa)

9.97

Isoelectric Point (pI)

33.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 670
AccB7I CCANNNNNTGG 1 cut(s) 263
AciI CCGC 2 cut(s) 11, 240
AcsI RAATTY 1 cut(s) 330
AfaI GTAC 2 cut(s) 366, 494
AfiI CCNNNNNNNGG 1 cut(s) 263
AflIII ACRYGT 1 cut(s) 281
AgsI TTSAA 6 cut(s) 199, 311, 607, 637, 644, 660
AhlI ACTAGT 1 cut(s) 184
AluBI AGCT 6 cut(s) 52, 89, 156, 305, 340, 560
AluI AGCT 6 cut(s) 52, 89, 156, 305, 340, 560
Alw26I GTCTC 1 cut(s) 581
ApeKI GCWGC 4 cut(s) 14, 153, 237, 449
ApoI RAATTY 1 cut(s) 330
Asp700I GAANNNNTTC 1 cut(s) 194
AspS9I GGNCC 2 cut(s) 75, 247
AvaII GGWCC 2 cut(s) 75, 247
BanII GRGCYC 1 cut(s) 487
BauI CACGAG 1 cut(s) 123
BbsI GAAGAC 1 cut(s) 527
BbvCI CCTCAGC 1 cut(s) 174
BbvI GCAGC 3 cut(s) 165, 249, 436
BccI CCATC 1 cut(s) 259
BcoDI GTCTC 1 cut(s) 581
BcuI ACTAGT 1 cut(s) 184
BfaI CTAG 3 cut(s) 150, 185, 525
BfmI CTRYAG 1 cut(s) 15
BisI GCNGC 5 cut(s) 12, 15, 154, 238, 450
BlsI GCNGC 5 cut(s) 13, 16, 155, 239, 451
BmcAI AGTACT 1 cut(s) 366
Bme18I GGWCC 2 cut(s) 75, 247
BmgT120I GGNCC 2 cut(s) 75, 247
BmiI GGNNCC 3 cut(s) 249, 484, 530
BmrI ACTGGG 1 cut(s) 442
BmsI GCATC 1 cut(s) 640
BmuI ACTGGG 1 cut(s) 442
BpiI GAAGAC 1 cut(s) 527
Bpu10I CCTNAGC 2 cut(s) 174, 341
BsaI GGTCTC 1 cut(s) 581
BsaJI CCNNGG 1 cut(s) 570
Bsc4I CCNNNNNNNGG 1 cut(s) 263
Bse1I ACTGG 2 cut(s) 277, 437
BseDI CCNNGG 1 cut(s) 570
BseGI GGATG 1 cut(s) 210
BseLI CCNNNNNNNGG 1 cut(s) 263
BseMII CTCAG 2 cut(s) 157, 188
BseNI ACTGG 2 cut(s) 277, 437
BseRI GAGGAG 1 cut(s) 111
BseXI GCAGC 3 cut(s) 165, 249, 436
BseYI CCCAGC 1 cut(s) 560
BslFI GGGAC 1 cut(s) 449
BslI CCNNNNNNNGG 1 cut(s) 263
BsmAI GTCTC 1 cut(s) 581
BsmFI GGGAC 1 cut(s) 449
Bso31I GGTCTC 1 cut(s) 581
Bsp1286I GDGCHC 1 cut(s) 487
Bsp1407I TGTACA 1 cut(s) 492
Bsp19I CCATGG 1 cut(s) 570
BspACI CCGC 2 cut(s) 11, 240
BspCNI CTCAG 2 cut(s) 156, 187
BspLI GGNNCC 3 cut(s) 249, 484, 530
BspMAI CTGCAG 1 cut(s) 19
BspTNI GGTCTC 1 cut(s) 581
BsrGI TGTACA 1 cut(s) 492
BsrI ACTGG 2 cut(s) 277, 437
BssECI CCNNGG 1 cut(s) 570
BssSI CACGAG 1 cut(s) 123
BssT1I CCWWGG 1 cut(s) 570
Bst2BI CACGAG 1 cut(s) 123
Bst4CI ACNGT 2 cut(s) 392, 412
Bst6I CTCTTC 3 cut(s) 374, 510, 584
BstAUI TGTACA 1 cut(s) 492
BstDEI CTNAG 4 cut(s) 143, 174, 341, 376
BstDSI CCRYGG 1 cut(s) 570
BstF5I GGATG 1 cut(s) 210
BstMAI GTCTC 1 cut(s) 581
BstMWI GCNNNNNNNGC 3 cut(s) 273, 302, 455
BstSFI CTRYAG 1 cut(s) 15
BstV1I GCAGC 3 cut(s) 165, 249, 436
BstV2I GAAGAC 1 cut(s) 527
BtgI CCRYGG 1 cut(s) 570
BtsCI GGATG 1 cut(s) 210
BtsI GCAGTG 1 cut(s) 232
BtsIMutI CAGTG 1 cut(s) 232
Cfr13I GGNCC 2 cut(s) 75, 247
Csp6I GTAC 2 cut(s) 365, 493
CspCI CAANNNNNGTGG 2 cut(s) 304, 339
CviAII CATG 5 cut(s) 106, 210, 490, 541, 571
CviQI GTAC 2 cut(s) 365, 493
DdeI CTNAG 4 cut(s) 143, 174, 341, 376
DraI TTTAAA 2 cut(s) 361, 595
Eam1104I CTCTTC 3 cut(s) 374, 510, 584
EarI CTCTTC 3 cut(s) 374, 510, 584
Eco130I CCWWGG 1 cut(s) 570
Eco24I GRGCYC 1 cut(s) 487
Eco31I GGTCTC 1 cut(s) 581
Eco47I GGWCC 2 cut(s) 75, 247
EcoT14I CCWWGG 1 cut(s) 570
EcoT22I ATGCAT 1 cut(s) 655
EcoT38I GRGCYC 1 cut(s) 487
ErhI CCWWGG 1 cut(s) 570
FaeI CATG 5 cut(s) 109, 213, 493, 544, 574
FaqI GGGAC 1 cut(s) 449
FatI CATG 5 cut(s) 105, 209, 489, 540, 570
FauI CCCGC 1 cut(s) 233
Fnu4HI GCNGC 5 cut(s) 12, 15, 154, 238, 450
FokI GGATG 1 cut(s) 217
FriOI GRGCYC 1 cut(s) 487
Fsp4HI GCNGC 5 cut(s) 12, 15, 154, 238, 450
FspBI CTAG 3 cut(s) 150, 185, 525
GluI GCNGC 5 cut(s) 12, 15, 154, 238, 450
GsaI CCCAGC 1 cut(s) 564
Hin1II CATG 5 cut(s) 109, 213, 493, 544, 574
HindIII AAGCTT 1 cut(s) 50
HinfI GANTC 2 cut(s) 587, 621
Hpy166II GTNNAC 2 cut(s) 190, 232
Hpy188I TCNGA 1 cut(s) 221
Hpy188III TCNNGA 3 cut(s) 78, 199, 335
Hpy8I GTNNAC 2 cut(s) 190, 232
HpyAV CCTTC 1 cut(s) 643
HpyCH4III ACNGT 2 cut(s) 392, 412
HpyCH4IV ACGT 1 cut(s) 283
HpyCH4V TGCA 4 cut(s) 17, 168, 395, 653
HpyF10VI GCNNNNNNNGC 3 cut(s) 273, 302, 455
HpyF3I CTNAG 4 cut(s) 143, 174, 341, 376
HpySE526I ACGT 1 cut(s) 283
Hsp92II CATG 5 cut(s) 109, 213, 493, 544, 574
LmnI GCTCC 2 cut(s) 482, 528
LpnPI CCDG 9 cut(s) 12, 58, 91, 149, 258, 411, 418, 545, 546
Lsp1109I GCAGC 3 cut(s) 165, 249, 436
LweI GCATC 1 cut(s) 640
MaeI CTAG 3 cut(s) 150, 185, 525
MaeII ACGT 1 cut(s) 283
MaeIII GTNAC 2 cut(s) 19, 29
MboII GAAGA 7 cut(s) 51, 154, 361, 364, 497, 532, 571
MhlI GDGCHC 1 cut(s) 487
MluCI AATT 5 cut(s) 193, 288, 330, 547, 637
MmeI TCCRAC 2 cut(s) 225, 281
MnlI CCTC 8 cut(s) 129, 132, 183, 189, 195, 341, 466, 497
Mph1103I ATGCAT 1 cut(s) 655
MroXI GAANNNNTTC 1 cut(s) 194
MseI TTAA 5 cut(s) 48, 287, 360, 546, 594
MspA1I CMGCKG 2 cut(s) 156, 240
MwoI GCNNNNNNNGC 3 cut(s) 273, 302, 455
NcoI CCATGG 1 cut(s) 570
NlaIII CATG 5 cut(s) 109, 213, 493, 544, 574
NlaIV GGNNCC 3 cut(s) 249, 484, 530
NsiI ATGCAT 1 cut(s) 655
PdmI GAANNNNTTC 1 cut(s) 194
PfeI GAWTC 2 cut(s) 587, 621
PflFI GACNNNGTC 1 cut(s) 211
PflMI CCANNNNNTGG 1 cut(s) 263
PkrI GCNGC 5 cut(s) 13, 16, 155, 239, 451
PsiI TTATAA 1 cut(s) 670
PspFI CCCAGC 1 cut(s) 560
PspN4I GGNNCC 3 cut(s) 249, 484, 530
PspPI GGNCC 2 cut(s) 75, 247
PstI CTGCAG 1 cut(s) 19
PsyI GACNNNGTC 1 cut(s) 211
PvuII CAGCTG 1 cut(s) 156
RsaI GTAC 2 cut(s) 366, 494
RsaNI GTAC 2 cut(s) 365, 493
SaqAI TTAA 5 cut(s) 48, 287, 360, 546, 594
SatI GCNGC 5 cut(s) 12, 15, 154, 238, 450
Sau96I GGNCC 2 cut(s) 75, 247
ScaI AGTACT 1 cut(s) 366
SduI GDGCHC 1 cut(s) 487
SfaNI GCATC 1 cut(s) 640
SfcI CTRYAG 1 cut(s) 15
SinI GGWCC 2 cut(s) 75, 247
SmiI ATTTAAAT 1 cut(s) 595
SpeI ACTAGT 1 cut(s) 184
Sse9I AATT 5 cut(s) 193, 288, 330, 547, 637
SsiI CCGC 2 cut(s) 11, 240
SspMI CTAG 3 cut(s) 150, 185, 525
StyI CCWWGG 1 cut(s) 570
SwaI ATTTAAAT 1 cut(s) 595
TaaI ACNGT 2 cut(s) 392, 412
TaiI ACGT 1 cut(s) 286
TaqI TCGA 1 cut(s) 334
TasI AATT 5 cut(s) 193, 288, 330, 547, 637
TatI WGTACW 2 cut(s) 364, 492
TauI GCSGC 1 cut(s) 14
TfiI GAWTC 2 cut(s) 587, 621
Tru1I TTAA 5 cut(s) 48, 287, 360, 546, 594
Tru9I TTAA 5 cut(s) 48, 287, 360, 546, 594
TscAI CASTG 1 cut(s) 232
TseI GCWGC 4 cut(s) 14, 153, 237, 449
TspDTI ATGAA 3 cut(s) 44, 533, 579
TspRI CASTG 1 cut(s) 232
Tth111I GACNNNGTC 1 cut(s) 211
Van91I CCANNNNNTGG 1 cut(s) 263
VpaK11BI GGWCC 2 cut(s) 75, 247
XapI RAATTY 1 cut(s) 330
XmnI GAANNNNTTC 1 cut(s) 194
XspI CTAG 3 cut(s) 150, 185, 525
ZrmI AGTACT 1 cut(s) 366
Zsp2I ATGCAT 1 cut(s) 655
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.