Rroxscaffold_4G00305370

Ycf20-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0008566
Physical Location & Seq
Reverse (-)
26022730 .. 26026179
3450 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00305370.1

Sequence Viewer

Length: 669 bp
ATGATAATGGCTACTGCAATTACAGTGCCTACCTATCCAAGAAATGTTAAGCTTTTATCTTCTAAGTGTGTTGGTCCCAGAGTTATAGCGCTGGGTCTTTGTGCAATGTCTTACAAACCTACTGGTACTTTTGGGCACAAGCTCAATTCTCGCTGCTGTTTCTTCTGTATGCCTGAACCTCTTCTAGTCAACAATTTCAAGAGGATTTCATGGTCTGTCAGAAGCAATGTAGATAACAGTGGGTTAGACCCATCTCCAACAAATGGCACTACTGGCACAAGATTAATTAGCGTTATCCAAGCTATTCAATCCAAGTTAGGTGCCAGAATTGGAGAGATAAGGAGAGGTTTACCCGTGAAAATACTTTTCTTCCTGGTCGGTTTCTATTGTTCAACTGCTTATGCTACTGTAATTGGGCAAACAGGAGACTGGGACATTCTCTCTGCGGCCTTTGCTGTGCTTATTGTGGAGGGGATTGGGGCCCTCATGTACAAGGCTTCTCTTCCTTTATTGAAGAAGACTAAAAGCCTGATAACCAAGTTTAATTATTGGAAAGCTGGGCTTGCAATGGGTCTTTTCTTGGATTCGTTTAAATATGAAGTCAATGACATTTTCGGATTCAGTAACCCCTTCAGTTTTGTACTAGATGCATTTTCATTATTCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF565

Protein Analysis

222

Amino Acids

24.35

Weight (kDa)

9.62

Isoelectric Point (pI)

30.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF565 PF04483 140 - 198 2.9e-16 Protein of unknown function (DUF565)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 320
AccB7I CCANNNNNTGG 1 cut(s) 263
AciI CCGC 1 cut(s) 446
AcuI CTGAAG 1 cut(s) 616
AfaI GTAC 3 cut(s) 127, 491, 642
AfeI AGCGCT 1 cut(s) 90
AfiI CCNNNNNNNGG 1 cut(s) 263
AgsI TTSAA 4 cut(s) 199, 308, 393, 514
AjnI CCWGG 1 cut(s) 372
AluBI AGCT 4 cut(s) 52, 142, 302, 557
AluI AGCT 4 cut(s) 52, 142, 302, 557
Alw26I GTCTC 1 cut(s) 420
Aor51HI AGCGCT 1 cut(s) 90
AoxI GGCC 2 cut(s) 447, 480
ApaI GGGCCC 1 cut(s) 484
ApeKI GCWGC 1 cut(s) 153
AseI ATTAAT 1 cut(s) 284
Asp700I GAANNNNTTC 1 cut(s) 180
AspLEI GCGC 1 cut(s) 91
AspS9I GGNCC 3 cut(s) 74, 480, 481
AvaII GGWCC 1 cut(s) 74
BaeGI GKGCMC 2 cut(s) 138, 484
BanI GGYRCC 1 cut(s) 320
BanII GRGCYC 1 cut(s) 484
BbsI GAAGAC 1 cut(s) 524
BbvI GCAGC 1 cut(s) 140
BccI CCATC 1 cut(s) 259
BciT130I CCWGG 1 cut(s) 374
BcoDI GTCTC 1 cut(s) 420
BfaI CTAG 2 cut(s) 185, 644
BfoI RGCGCY 1 cut(s) 92
BisI GCNGC 2 cut(s) 154, 447
BlsI GCNGC 2 cut(s) 155, 448
Bme1390I CCNGG 1 cut(s) 374
Bme18I GGWCC 1 cut(s) 74
BmgT120I GGNCC 3 cut(s) 74, 480, 481
BmiI GGNNCC 4 cut(s) 76, 322, 481, 482
BmrFI CCNGG 1 cut(s) 374
BmrI ACTGGG 1 cut(s) 439
BmsI GCATC 1 cut(s) 637
BmuI ACTGGG 1 cut(s) 439
BpiI GAAGAC 1 cut(s) 524
Bsc4I CCNNNNNNNGG 1 cut(s) 263
Bse1I ACTGG 3 cut(s) 127, 277, 434
Bse3DI GCAATG 3 cut(s) 111, 232, 573
BseBI CCWGG 1 cut(s) 374
BseLI CCNNNNNNNGG 1 cut(s) 263
BseMI GCAATG 3 cut(s) 111, 232, 573
BseNI ACTGG 3 cut(s) 127, 277, 434
BseSI GKGCMC 2 cut(s) 138, 484
BseXI GCAGC 1 cut(s) 140
BseYI CCCAGC 2 cut(s) 91, 557
BshFI GGCC 2 cut(s) 449, 482
BshNI GGYRCC 1 cut(s) 320
BslFI GGGAC 2 cut(s) 60, 446
BslI CCNNNNNNNGG 1 cut(s) 263
BsmAI GTCTC 1 cut(s) 420
BsmFI GGGAC 2 cut(s) 60, 446
BsnI GGCC 2 cut(s) 449, 482
Bsp120I GGGCCC 1 cut(s) 480
Bsp1286I GDGCHC 2 cut(s) 138, 484
Bsp1407I TGTACA 1 cut(s) 489
BspACI CCGC 1 cut(s) 446
BspANI GGCC 2 cut(s) 449, 482
BspLI GGNNCC 4 cut(s) 76, 322, 481, 482
BspT107I GGYRCC 1 cut(s) 320
BsrDI GCAATG 3 cut(s) 111, 232, 573
BsrGI TGTACA 1 cut(s) 489
BsrI ACTGG 3 cut(s) 127, 277, 434
Bst2UI CCWGG 1 cut(s) 374
Bst4CI ACNGT 3 cut(s) 25, 239, 409
Bst6I CTCTTC 2 cut(s) 186, 507
BstAUI TGTACA 1 cut(s) 489
BstC8I GCNNGC 1 cut(s) 564
BstDEI CTNAG 1 cut(s) 63
BstH2I RGCGCY 1 cut(s) 92
BstHHI GCGC 1 cut(s) 91
BstMAI GTCTC 1 cut(s) 420
BstMWI GCNNNNNNNGC 3 cut(s) 273, 452, 563
BstNI CCWGG 1 cut(s) 374
BstSCI CCNGG 1 cut(s) 372
BstSLI GKGCMC 2 cut(s) 138, 484
BstV1I GCAGC 1 cut(s) 140
BstV2I GAAGAC 1 cut(s) 524
BsuRI GGCC 2 cut(s) 449, 482
BtsIMutI CAGTG 2 cut(s) 30, 244
Cac8I GCNNGC 1 cut(s) 564
CfoI GCGC 1 cut(s) 91
Cfr13I GGNCC 3 cut(s) 74, 480, 481
Csp6I GTAC 3 cut(s) 126, 490, 641
CviAII CATG 2 cut(s) 210, 487
CviQI GTAC 3 cut(s) 126, 490, 641
DdeI CTNAG 1 cut(s) 63
DraI TTTAAA 1 cut(s) 592
Eam1104I CTCTTC 2 cut(s) 186, 507
EarI CTCTTC 2 cut(s) 186, 507
Eco24I GRGCYC 1 cut(s) 484
Eco47I GGWCC 1 cut(s) 74
Eco47III AGCGCT 1 cut(s) 90
Eco57I CTGAAG 1 cut(s) 616
EcoO109I RGGNCCY 2 cut(s) 480, 481
EcoRII CCWGG 1 cut(s) 372
EcoT22I ATGCAT 1 cut(s) 652
EcoT38I GRGCYC 1 cut(s) 484
FaeI CATG 2 cut(s) 213, 490
FaiI YATR 6 cut(s) 86, 170, 211, 402, 488, 597
FalI AAGNNNNNCTT 2 cut(s) 546, 578
FaqI GGGAC 2 cut(s) 60, 446
FatI CATG 2 cut(s) 209, 486
Fnu4HI GCNGC 2 cut(s) 154, 447
FriOI GRGCYC 1 cut(s) 484
Fsp4HI GCNGC 2 cut(s) 154, 447
FspBI CTAG 2 cut(s) 185, 644
GlaI GCGC 1 cut(s) 90
GluI GCNGC 2 cut(s) 154, 447
GsaI CCCAGC 2 cut(s) 95, 561
HaeII RGCGCY 1 cut(s) 92
HaeIII GGCC 2 cut(s) 449, 482
HhaI GCGC 1 cut(s) 91
Hin1II CATG 2 cut(s) 213, 490
Hin6I GCGC 1 cut(s) 89
HinP1I GCGC 1 cut(s) 89
HincII GTYRAC 1 cut(s) 190
HindII GTYRAC 1 cut(s) 190
HindIII AAGCTT 1 cut(s) 50
HinfI GANTC 2 cut(s) 584, 618
Hpy166II GTNNAC 2 cut(s) 190, 350
Hpy188I TCNGA 2 cut(s) 221, 617
Hpy188III TCNNGA 1 cut(s) 199
Hpy8I GTNNAC 2 cut(s) 190, 350
HpyAV CCTTC 1 cut(s) 640
HpyCH4III ACNGT 3 cut(s) 25, 239, 409
HpyCH4V TGCA 4 cut(s) 17, 104, 566, 650
HpyF10VI GCNNNNNNNGC 3 cut(s) 273, 452, 563
HpyF3I CTNAG 1 cut(s) 63
Hsp92II CATG 2 cut(s) 213, 490
HspAI GCGC 1 cut(s) 89
Lsp1109I GCAGC 1 cut(s) 140
LweI GCATC 1 cut(s) 637
MaeI CTAG 2 cut(s) 185, 644
MaeIII GTNAC 1 cut(s) 623
MboII GAAGA 7 cut(s) 51, 154, 173, 361, 494, 526, 529
MhlI GDGCHC 2 cut(s) 138, 484
MluCI AATT 7 cut(s) 18, 145, 193, 285, 327, 411, 544
MmeI TCCRAC 1 cut(s) 281
MnlI CCTC 5 cut(s) 189, 195, 338, 463, 494
Mph1103I ATGCAT 1 cut(s) 652
MroXI GAANNNNTTC 1 cut(s) 180
MseI TTAA 5 cut(s) 48, 284, 543, 591, 667
MspR9I CCNGG 1 cut(s) 374
MvaI CCWGG 1 cut(s) 374
MwoI GCNNNNNNNGC 3 cut(s) 273, 452, 563
NlaIII CATG 2 cut(s) 213, 490
NlaIV GGNNCC 4 cut(s) 76, 322, 481, 482
NsiI ATGCAT 1 cut(s) 652
PdmI GAANNNNTTC 1 cut(s) 180
PfeI GAWTC 2 cut(s) 584, 618
PflMI CCANNNNNTGG 1 cut(s) 263
PkrI GCNGC 2 cut(s) 155, 448
PshBI ATTAAT 1 cut(s) 284
Psp6I CCWGG 1 cut(s) 372
PspFI CCCAGC 2 cut(s) 91, 557
PspGI CCWGG 1 cut(s) 372
PspN4I GGNNCC 4 cut(s) 76, 322, 481, 482
PspOMI GGGCCC 1 cut(s) 480
PspPI GGNCC 3 cut(s) 74, 480, 481
RsaI GTAC 3 cut(s) 127, 491, 642
RsaNI GTAC 3 cut(s) 126, 490, 641
SaqAI TTAA 5 cut(s) 48, 284, 543, 591, 667
SatI GCNGC 2 cut(s) 154, 447
Sau96I GGNCC 3 cut(s) 74, 480, 481
ScrFI CCNGG 1 cut(s) 374
SduI GDGCHC 2 cut(s) 138, 484
SetI ASST 9 cut(s) 35, 54, 121, 144, 181, 304, 322, 349, 559
SfaNI GCATC 1 cut(s) 637
SinI GGWCC 1 cut(s) 74
Sse9I AATT 7 cut(s) 18, 145, 193, 285, 327, 411, 544
SsiI CCGC 1 cut(s) 446
SspMI CTAG 2 cut(s) 185, 644
StyD4I CCNGG 1 cut(s) 372
TaaI ACNGT 3 cut(s) 25, 239, 409
TasI AATT 7 cut(s) 18, 145, 193, 285, 327, 411, 544
TatI WGTACW 2 cut(s) 489, 640
TauI GCSGC 1 cut(s) 449
TfiI GAWTC 2 cut(s) 584, 618
Tru1I TTAA 5 cut(s) 48, 284, 543, 591, 667
Tru9I TTAA 5 cut(s) 48, 284, 543, 591, 667
TscAI CASTG 2 cut(s) 30, 244
TseI GCWGC 1 cut(s) 153
TspDTI ATGAA 3 cut(s) 198, 612, 645
TspRI CASTG 2 cut(s) 30, 244
Van91I CCANNNNNTGG 1 cut(s) 263
VpaK11BI GGWCC 1 cut(s) 74
VspI ATTAAT 1 cut(s) 284
XmnI GAANNNNTTC 1 cut(s) 180
XspI CTAG 2 cut(s) 185, 644
Zsp2I ATGCAT 1 cut(s) 652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.