MD07G1118800.v1.1

Ycf20-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0008566
Physical Location & Seq
Forward (+)
14838724 .. 14841308
2585 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1118800.v1.1.491

Sequence Viewer

Length: 561 bp
ATGCTTCACAAACCTTCTCATGCTTTTGGCCAAAAACTCAGTTCTCGTTGCTGTTTATTCTGCAGACCTCAACCTCTACTGGTCAACAATTTCAAGAGGATGACATGGTCTATCAGAAGTAATAGCAGTGGGTTGGATCCATCTCCAAGAAATGGCTCTACTGGCACGACGCGATTAATTAGGGCAATCCAAGCTATTCAAACCAAGTTAGGTGTAAAAATTCGACAGCTACGGAGAGGTTTTCCAATGAAATTACTCTTCTTCTTAGTGGGTTTCTACTGTGCTACTGCTTATGCTACTGTTATTGGGCAAACAGGCGACTGGGACATTCTATCTGCCGCTTTTGCTGTGGTTATTGTGGAGGGGATTGGGGCACTCATGTATAAGGCGTCTATTCCTTTTGTAAAGAAGACTAGGAACCTGATAACCATGTTTAACTATTGGAAAGCTGGGCTTTCCATGGGTCTCTTCTTGGATTCATTCAAATATGAGTTCAATGACATCTTTGGATTCAGTAACCCCTTCAATTTCAAACTACACGCTTTCTCAATATTCTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF565

Protein Analysis

187

Amino Acids

21.1

Weight (kDa)

10.26

Isoelectric Point (pI)

28.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF565 PF04483 104 - 162 6.6e-17 Protein of unknown function (DUF565)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 152
AccII CGCG 1 cut(s) 172
AciI CCGC 1 cut(s) 339
AclWI GGATC 2 cut(s) 131, 144
AcoI YGGCCR 1 cut(s) 28
AcsI RAATTY 1 cut(s) 219
AcyI GRCGYC 1 cut(s) 389
AfiI CCNNNNNNNGG 1 cut(s) 152
AgsI TTSAA 6 cut(s) 94, 200, 484, 496, 526, 532
AluBI AGCT 3 cut(s) 194, 229, 449
AluI AGCT 3 cut(s) 194, 229, 449
Alw26I GTCTC 1 cut(s) 470
AlwI GGATC 2 cut(s) 131, 144
AoxI GGCC 1 cut(s) 28
ApoI RAATTY 1 cut(s) 219
AseI ATTAAT 1 cut(s) 176
BaeGI GKGCMC 1 cut(s) 376
BalI TGGCCA 1 cut(s) 30
BamHI GGATCC 1 cut(s) 136
BbsI GAAGAC 1 cut(s) 416
BccI CCATC 1 cut(s) 148
BcoDI GTCTC 1 cut(s) 470
BfaI CTAG 1 cut(s) 414
BfmI CTRYAG 1 cut(s) 61
BisI GCNGC 1 cut(s) 339
BlsI GCNGC 1 cut(s) 340
BmiI GGNNCC 2 cut(s) 138, 419
BmrI ACTGGG 1 cut(s) 331
BmuI ACTGGG 1 cut(s) 331
BpiI GAAGAC 1 cut(s) 416
BsaHI GRCGYC 1 cut(s) 389
BsaI GGTCTC 1 cut(s) 470
BsaJI CCNNGG 1 cut(s) 459
Bsc4I CCNNNNNNNGG 1 cut(s) 152
Bse1I ACTGG 3 cut(s) 84, 166, 326
BseDI CCNNGG 1 cut(s) 459
BseGI GGATG 1 cut(s) 105
BseLI CCNNNNNNNGG 1 cut(s) 152
BseMII CTCAG 1 cut(s) 52
BseNI ACTGG 3 cut(s) 84, 166, 326
BseSI GKGCMC 1 cut(s) 376
BseYI CCCAGC 1 cut(s) 449
Bsh1236I CGCG 1 cut(s) 172
BshFI GGCC 1 cut(s) 30
BslFI GGGAC 1 cut(s) 338
BslI CCNNNNNNNGG 1 cut(s) 152
BsmAI GTCTC 1 cut(s) 470
BsmFI GGGAC 1 cut(s) 338
BsnI GGCC 1 cut(s) 30
Bso31I GGTCTC 1 cut(s) 470
Bsp1286I GDGCHC 1 cut(s) 376
Bsp143I GATC 1 cut(s) 136
Bsp19I CCATGG 1 cut(s) 459
BspACI CCGC 1 cut(s) 339
BspANI GGCC 1 cut(s) 30
BspCNI CTCAG 1 cut(s) 51
BspFNI CGCG 1 cut(s) 172
BspLI GGNNCC 2 cut(s) 138, 419
BspMAI CTGCAG 1 cut(s) 65
BspPI GGATC 2 cut(s) 131, 144
BspTNI GGTCTC 1 cut(s) 470
BsrI ACTGG 3 cut(s) 84, 166, 326
BssECI CCNNGG 1 cut(s) 459
BssMI GATC 1 cut(s) 136
BssNI GRCGYC 1 cut(s) 389
BssT1I CCWWGG 1 cut(s) 459
Bst4CI ACNGT 2 cut(s) 281, 301
Bst6I CTCTTC 2 cut(s) 263, 473
BstACI GRCGYC 1 cut(s) 389
BstDEI CTNAG 2 cut(s) 38, 265
BstDSI CCRYGG 1 cut(s) 459
BstF5I GGATG 1 cut(s) 105
BstFNI CGCG 1 cut(s) 172
BstKTI GATC 1 cut(s) 139
BstMAI GTCTC 1 cut(s) 470
BstMBI GATC 1 cut(s) 136
BstMWI GCNNNNNNNGC 3 cut(s) 162, 191, 344
BstSFI CTRYAG 1 cut(s) 61
BstSLI GKGCMC 1 cut(s) 376
BstUI CGCG 1 cut(s) 172
BstV2I GAAGAC 1 cut(s) 416
BstX2I RGATCY 1 cut(s) 136
BstYI RGATCY 1 cut(s) 136
BsuRI GGCC 1 cut(s) 30
BtgI CCRYGG 1 cut(s) 459
BtsCI GGATG 1 cut(s) 105
BtsI GCAGTG 1 cut(s) 133
BtsIMutI CAGTG 1 cut(s) 133
CseI GACGC 2 cut(s) 178, 378
CviAII CATG 5 cut(s) 20, 105, 379, 430, 460
CviJI RGCY 6 cut(s) 30, 156, 194, 229, 449, 454
CviKI_1 RGCY 6 cut(s) 30, 156, 194, 229, 449, 454
DdeI CTNAG 2 cut(s) 38, 265
DpnI GATC 1 cut(s) 138
DpnII GATC 1 cut(s) 136
EaeI YGGCCR 1 cut(s) 28
Eam1104I CTCTTC 2 cut(s) 263, 473
EarI CTCTTC 2 cut(s) 263, 473
Eco130I CCWWGG 1 cut(s) 459
Eco31I GGTCTC 1 cut(s) 470
EcoT14I CCWWGG 1 cut(s) 459
ErhI CCWWGG 1 cut(s) 459
FaeI CATG 5 cut(s) 23, 108, 382, 433, 463
FaiI YATR 8 cut(s) 21, 106, 294, 380, 384, 431, 461, 489
FalI AAGNNNNNCTT 2 cut(s) 438, 470
FaqI GGGAC 1 cut(s) 338
FatI CATG 5 cut(s) 19, 104, 378, 429, 459
Fnu4HI GCNGC 1 cut(s) 339
FokI GGATG 1 cut(s) 112
Fsp4HI GCNGC 1 cut(s) 339
FspBI CTAG 1 cut(s) 414
GluI GCNGC 1 cut(s) 339
GsaI CCCAGC 1 cut(s) 453
HaeIII GGCC 1 cut(s) 30
HgaI GACGC 2 cut(s) 178, 378
Hin1I GRCGYC 1 cut(s) 389
Hin1II CATG 5 cut(s) 23, 108, 382, 433, 463
HincII GTYRAC 1 cut(s) 85
HindII GTYRAC 1 cut(s) 85
HinfI GANTC 2 cut(s) 476, 510
Hpy166II GTNNAC 1 cut(s) 85
Hpy188I TCNGA 1 cut(s) 116
Hpy188III TCNNGA 1 cut(s) 94
Hpy8I GTNNAC 1 cut(s) 85
Hpy99I CGWCG 1 cut(s) 172
HpyAV CCTTC 2 cut(s) 24, 532
HpyCH4III ACNGT 2 cut(s) 281, 301
HpyCH4V TGCA 1 cut(s) 63
HpyF10VI GCNNNNNNNGC 3 cut(s) 162, 191, 344
HpyF3I CTNAG 2 cut(s) 38, 265
Hsp92I GRCGYC 1 cut(s) 389
Hsp92II CATG 5 cut(s) 23, 108, 382, 433, 463
Kzo9I GATC 1 cut(s) 136
LpnPI CCDG 7 cut(s) 65, 147, 300, 307, 434, 435, 541
MaeI CTAG 1 cut(s) 414
MaeIII GTNAC 1 cut(s) 515
MalI GATC 1 cut(s) 138
MboI GATC 1 cut(s) 136
MboII GAAGA 4 cut(s) 250, 253, 421, 460
MflI RGATCY 1 cut(s) 136
MhlI GDGCHC 1 cut(s) 376
MlsI TGGCCA 1 cut(s) 30
MluCI AATT 5 cut(s) 88, 177, 219, 251, 526
MluNI TGGCCA 1 cut(s) 30
MmeI TCCRAC 1 cut(s) 114
MnlI CCTC 5 cut(s) 78, 84, 90, 230, 355
Mox20I TGGCCA 1 cut(s) 30
MscI TGGCCA 1 cut(s) 30
MseI TTAA 2 cut(s) 176, 435
Msp20I TGGCCA 1 cut(s) 30
MvnI CGCG 1 cut(s) 172
MwoI GCNNNNNNNGC 3 cut(s) 162, 191, 344
NcoI CCATGG 1 cut(s) 459
NdeII GATC 1 cut(s) 136
NlaIII CATG 5 cut(s) 23, 108, 382, 433, 463
NlaIV GGNNCC 2 cut(s) 138, 419
PfeI GAWTC 2 cut(s) 476, 510
PflFI GACNNNGTC 1 cut(s) 106
PflMI CCANNNNNTGG 1 cut(s) 152
PkrI GCNGC 1 cut(s) 340
PshBI ATTAAT 1 cut(s) 176
PspFI CCCAGC 1 cut(s) 449
PspN4I GGNNCC 2 cut(s) 138, 419
PstI CTGCAG 1 cut(s) 65
PsuI RGATCY 1 cut(s) 136
PsyI GACNNNGTC 1 cut(s) 106
SaqAI TTAA 2 cut(s) 176, 435
SatI GCNGC 1 cut(s) 339
Sau3AI GATC 1 cut(s) 136
SduI GDGCHC 1 cut(s) 376
SetI ASST 9 cut(s) 16, 70, 76, 196, 214, 231, 241, 423, 451
SfcI CTRYAG 1 cut(s) 61
Sse9I AATT 5 cut(s) 88, 177, 219, 251, 526
SsiI CCGC 1 cut(s) 339
SspI AATATT 1 cut(s) 552
SspMI CTAG 1 cut(s) 414
StyI CCWWGG 1 cut(s) 459
TaaI ACNGT 2 cut(s) 281, 301
TaqI TCGA 1 cut(s) 223
TasI AATT 5 cut(s) 88, 177, 219, 251, 526
TauI GCSGC 1 cut(s) 341
TfiI GAWTC 2 cut(s) 476, 510
Tru1I TTAA 2 cut(s) 176, 435
Tru9I TTAA 2 cut(s) 176, 435
TscAI CASTG 1 cut(s) 133
TspDTI ATGAA 2 cut(s) 263, 468
TspGWI ACGGA 1 cut(s) 247
TspRI CASTG 1 cut(s) 133
Tth111I GACNNNGTC 1 cut(s) 106
Van91I CCANNNNNTGG 1 cut(s) 152
VspI ATTAAT 1 cut(s) 176
XapI RAATTY 1 cut(s) 219
XspI CTAG 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.