Rh1CG207400

Ycf20-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0008566
Physical Location & Seq
Forward (+)
44924704 .. 44926524
1821 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG207400.1

Sequence Viewer

Length: 750 bp
ATGGGTCGTGGGCCGGCTGAAGCAGAGCTGATTCTCCATCCCAAGCTTATTTTGTGTGAGAGCCTCAAACTGTTGTATATGATAATGGCGACTGCAATTACAGTGCCTACCTATCCAAGAAATGTTAAGCTTTTATCTTCTAAGAGTGTTGGTTCCAGAGTTATAGCGCTGGGTCTTTGTGCAATGTCTTACAAACCCACTAGTACTTTTGGGCACAAGCTCAATTCTCGCTGCTGTTTCTTCTGTATGCCTGAACCTCTTCTAGTCAACAATTTCAAGAGGATTTCATGGTCTGTCAGAAGCAGTGTAGATAACAGTGGGTTAGACCCATCTCCAACAAATGGCACTACAGGCACAACCAGATTAATTAGGGTTATCCAAGCTATTCAATCCAAGTTAGGTGCCAGAATTGGAGAGATAAGGAGAGGTTTACCCGTGAAAATACTTTTCTTCCTAGTCGGTTTCTATTGTTCAACTGCTTATGCTACTGTTATTGGGCAAACGGGAGACTGGGACATTCTCTCTGCGGCCTTGGCTGTGCTTATTGTGGAGGGGATTGGGGCCCTCATGTACAAGGCTTCTCTTCCTTTATTGAAGAAGACTAAAAGCCTGATAACCAAGTTTAATTATTGGAAAGCTGGGCTTGCGATGGGTCTTTTCTTGGATTCGTTTAAATATGAAGTCAATGACATTTTCGGATTCAGTAACCCCTTCAGTTTTGTACTAGATGCATTTTCATTATTCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF565

Protein Analysis

249

Amino Acids

27.36

Weight (kDa)

9.62

Isoelectric Point (pI)

34.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF565 PF04483 167 - 225 6.7e-16 Protein of unknown function (DUF565)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 401
AccB7I CCANNNNNTGG 1 cut(s) 341
AciI CCGC 1 cut(s) 527
AcuI CTGAAG 2 cut(s) 39, 697
AfaI GTAC 3 cut(s) 205, 572, 723
AfeI AGCGCT 1 cut(s) 168
AfiI CCNNNNNNNGG 1 cut(s) 341
AgsI TTSAA 4 cut(s) 277, 389, 474, 595
AhlI ACTAGT 1 cut(s) 200
AluBI AGCT 6 cut(s) 28, 46, 130, 220, 383, 638
AluI AGCT 6 cut(s) 28, 46, 130, 220, 383, 638
Alw26I GTCTC 1 cut(s) 501
Aor51HI AGCGCT 1 cut(s) 168
AoxI GGCC 3 cut(s) 11, 528, 561
ApaI GGGCCC 1 cut(s) 565
ApeKI GCWGC 1 cut(s) 231
AseI ATTAAT 1 cut(s) 365
Asp700I GAANNNNTTC 1 cut(s) 258
AspLEI GCGC 1 cut(s) 169
AspS9I GGNCC 3 cut(s) 11, 561, 562
BaeGI GKGCMC 2 cut(s) 216, 565
BanI GGYRCC 1 cut(s) 401
BanII GRGCYC 1 cut(s) 565
BbsI GAAGAC 1 cut(s) 605
BbvI GCAGC 1 cut(s) 218
BccI CCATC 3 cut(s) 45, 337, 643
BcoDI GTCTC 1 cut(s) 501
BcuI ACTAGT 1 cut(s) 200
BfaI CTAG 4 cut(s) 201, 263, 455, 725
BfmI CTRYAG 1 cut(s) 348
BfoI RGCGCY 1 cut(s) 170
BisI GCNGC 2 cut(s) 232, 528
BlsI GCNGC 2 cut(s) 233, 529
BmcAI AGTACT 1 cut(s) 205
BmgT120I GGNCC 3 cut(s) 11, 561, 562
BmiI GGNNCC 4 cut(s) 154, 403, 562, 563
BmrI ACTGGG 1 cut(s) 520
BmsI GCATC 1 cut(s) 718
BmuI ACTGGG 1 cut(s) 520
BpiI GAAGAC 1 cut(s) 605
BsaJI CCNNGG 1 cut(s) 531
Bsc4I CCNNNNNNNGG 1 cut(s) 341
Bse118I RCCGGY 1 cut(s) 13
Bse1I ACTGG 1 cut(s) 515
Bse3DI GCAATG 1 cut(s) 189
BseDI CCNNGG 1 cut(s) 531
BseGI GGATG 1 cut(s) 37
BseLI CCNNNNNNNGG 1 cut(s) 341
BseMI GCAATG 1 cut(s) 189
BseNI ACTGG 1 cut(s) 515
BseSI GKGCMC 2 cut(s) 216, 565
BseXI GCAGC 1 cut(s) 218
BseYI CCCAGC 2 cut(s) 169, 638
BshFI GGCC 3 cut(s) 13, 530, 563
BshNI GGYRCC 1 cut(s) 401
BsiSI CCGG 1 cut(s) 14
BslFI GGGAC 1 cut(s) 527
BslI CCNNNNNNNGG 1 cut(s) 341
BsmAI GTCTC 1 cut(s) 501
BsmFI GGGAC 1 cut(s) 527
BsnI GGCC 3 cut(s) 13, 530, 563
Bsp120I GGGCCC 1 cut(s) 561
Bsp1286I GDGCHC 2 cut(s) 216, 565
Bsp1407I TGTACA 1 cut(s) 570
BspACI CCGC 1 cut(s) 527
BspANI GGCC 3 cut(s) 13, 530, 563
BspLI GGNNCC 4 cut(s) 154, 403, 562, 563
BspT107I GGYRCC 1 cut(s) 401
BsrDI GCAATG 1 cut(s) 189
BsrFI RCCGGY 1 cut(s) 13
BsrGI TGTACA 1 cut(s) 570
BsrI ACTGG 1 cut(s) 515
BssAI RCCGGY 1 cut(s) 13
BssECI CCNNGG 1 cut(s) 531
BssT1I CCWWGG 1 cut(s) 531
Bst4CI ACNGT 4 cut(s) 72, 103, 317, 490
Bst6I CTCTTC 2 cut(s) 264, 588
BstAUI TGTACA 1 cut(s) 570
BstC8I GCNNGC 2 cut(s) 15, 645
BstDEI CTNAG 1 cut(s) 141
BstF5I GGATG 1 cut(s) 37
BstH2I RGCGCY 1 cut(s) 170
BstHHI GCGC 1 cut(s) 169
BstMAI GTCTC 1 cut(s) 501
BstMWI GCNNNNNNNGC 3 cut(s) 351, 533, 644
BstSFI CTRYAG 1 cut(s) 348
BstSLI GKGCMC 2 cut(s) 216, 565
BstV1I GCAGC 1 cut(s) 218
BstV2I GAAGAC 1 cut(s) 605
BsuRI GGCC 3 cut(s) 13, 530, 563
BtgZI GCGATG 1 cut(s) 662
BtsCI GGATG 1 cut(s) 37
BtsI GCAGTG 1 cut(s) 310
BtsIMutI CAGTG 3 cut(s) 108, 310, 322
Cac8I GCNNGC 2 cut(s) 15, 645
CfoI GCGC 1 cut(s) 169
Cfr10I RCCGGY 1 cut(s) 13
Cfr13I GGNCC 3 cut(s) 11, 561, 562
Csp6I GTAC 3 cut(s) 204, 571, 722
CviAII CATG 2 cut(s) 288, 568
CviQI GTAC 3 cut(s) 204, 571, 722
DdeI CTNAG 1 cut(s) 141
DraI TTTAAA 1 cut(s) 673
Eam1104I CTCTTC 2 cut(s) 264, 588
EarI CTCTTC 2 cut(s) 264, 588
Eco130I CCWWGG 1 cut(s) 531
Eco24I GRGCYC 1 cut(s) 565
Eco47III AGCGCT 1 cut(s) 168
Eco57I CTGAAG 2 cut(s) 39, 697
EcoO109I RGGNCCY 2 cut(s) 561, 562
EcoT14I CCWWGG 1 cut(s) 531
EcoT22I ATGCAT 1 cut(s) 733
EcoT38I GRGCYC 1 cut(s) 565
ErhI CCWWGG 1 cut(s) 531
FaeI CATG 2 cut(s) 291, 571
FaiI YATR 8 cut(s) 78, 80, 164, 248, 289, 483, 569, 678
FalI AAGNNNNNCTT 2 cut(s) 627, 659
FaqI GGGAC 1 cut(s) 527
FatI CATG 2 cut(s) 287, 567
Fnu4HI GCNGC 2 cut(s) 232, 528
FokI GGATG 1 cut(s) 24
FriOI GRGCYC 1 cut(s) 565
Fsp4HI GCNGC 2 cut(s) 232, 528
FspBI CTAG 4 cut(s) 201, 263, 455, 725
GlaI GCGC 1 cut(s) 168
GluI GCNGC 2 cut(s) 232, 528
GsaI CCCAGC 2 cut(s) 173, 642
HaeII RGCGCY 1 cut(s) 170
HaeIII GGCC 3 cut(s) 13, 530, 563
HapII CCGG 1 cut(s) 14
HhaI GCGC 1 cut(s) 169
Hin1II CATG 2 cut(s) 291, 571
Hin6I GCGC 1 cut(s) 167
HinP1I GCGC 1 cut(s) 167
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HindIII AAGCTT 2 cut(s) 44, 128
HinfI GANTC 3 cut(s) 31, 665, 699
HpaII CCGG 1 cut(s) 14
Hpy166II GTNNAC 2 cut(s) 268, 431
Hpy188I TCNGA 2 cut(s) 299, 698
Hpy188III TCNNGA 2 cut(s) 156, 277
Hpy8I GTNNAC 2 cut(s) 268, 431
HpyAV CCTTC 1 cut(s) 721
HpyCH4III ACNGT 4 cut(s) 72, 103, 317, 490
HpyCH4V TGCA 3 cut(s) 95, 182, 731
HpyF10VI GCNNNNNNNGC 3 cut(s) 351, 533, 644
HpyF3I CTNAG 1 cut(s) 141
Hsp92II CATG 2 cut(s) 291, 571
HspAI GCGC 1 cut(s) 167
KroI GCCGGC 1 cut(s) 13
KroNI GCCGGC 1 cut(s) 15
Lsp1109I GCAGC 1 cut(s) 218
LweI GCATC 1 cut(s) 718
MaeI CTAG 4 cut(s) 201, 263, 455, 725
MaeIII GTNAC 1 cut(s) 704
MboII GAAGA 7 cut(s) 129, 232, 251, 442, 575, 607, 610
MhlI GDGCHC 2 cut(s) 216, 565
MluCI AATT 6 cut(s) 96, 223, 271, 366, 408, 625
MmeI TCCRAC 1 cut(s) 359
MnlI CCTC 6 cut(s) 74, 267, 273, 419, 544, 575
Mph1103I ATGCAT 1 cut(s) 733
MroNI GCCGGC 1 cut(s) 13
MroXI GAANNNNTTC 1 cut(s) 258
MseI TTAA 5 cut(s) 126, 365, 624, 672, 748
MspI CCGG 1 cut(s) 14
MwoI GCNNNNNNNGC 3 cut(s) 351, 533, 644
NaeI GCCGGC 1 cut(s) 15
NgoMIV GCCGGC 1 cut(s) 13
NlaIII CATG 2 cut(s) 291, 571
NlaIV GGNNCC 4 cut(s) 154, 403, 562, 563
NsiI ATGCAT 1 cut(s) 733
PdiI GCCGGC 1 cut(s) 15
PdmI GAANNNNTTC 1 cut(s) 258
PfeI GAWTC 3 cut(s) 31, 665, 699
PflMI CCANNNNNTGG 1 cut(s) 341
PkrI GCNGC 2 cut(s) 233, 529
PshBI ATTAAT 1 cut(s) 365
PspFI CCCAGC 2 cut(s) 169, 638
PspN4I GGNNCC 4 cut(s) 154, 403, 562, 563
PspOMI GGGCCC 1 cut(s) 561
PspPI GGNCC 3 cut(s) 11, 561, 562
RsaI GTAC 3 cut(s) 205, 572, 723
RsaNI GTAC 3 cut(s) 204, 571, 722
SaqAI TTAA 5 cut(s) 126, 365, 624, 672, 748
SatI GCNGC 2 cut(s) 232, 528
Sau96I GGNCC 3 cut(s) 11, 561, 562
ScaI AGTACT 1 cut(s) 205
SduI GDGCHC 2 cut(s) 216, 565
SfaNI GCATC 1 cut(s) 718
SfcI CTRYAG 1 cut(s) 348
SpeI ACTAGT 1 cut(s) 200
Sse9I AATT 6 cut(s) 96, 223, 271, 366, 408, 625
SsiI CCGC 1 cut(s) 527
SspMI CTAG 4 cut(s) 201, 263, 455, 725
StyI CCWWGG 1 cut(s) 531
TaaI ACNGT 4 cut(s) 72, 103, 317, 490
TasI AATT 6 cut(s) 96, 223, 271, 366, 408, 625
TatI WGTACW 3 cut(s) 203, 570, 721
TauI GCSGC 1 cut(s) 530
TfiI GAWTC 3 cut(s) 31, 665, 699
Tru1I TTAA 5 cut(s) 126, 365, 624, 672, 748
Tru9I TTAA 5 cut(s) 126, 365, 624, 672, 748
TscAI CASTG 3 cut(s) 108, 310, 322
TseI GCWGC 1 cut(s) 231
TspDTI ATGAA 3 cut(s) 276, 693, 726
TspRI CASTG 3 cut(s) 108, 310, 322
Van91I CCANNNNNTGG 1 cut(s) 341
VspI ATTAAT 1 cut(s) 365
XmnI GAANNNNTTC 1 cut(s) 258
XspI CTAG 4 cut(s) 201, 263, 455, 725
ZrmI AGTACT 1 cut(s) 205
Zsp2I ATGCAT 1 cut(s) 733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.