AT3G61250
MYB Family

myb domain protein 17

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0009623
Physical Location & Seq
Forward (+)
22670729 .. 22672751
2023 bp
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UTR
Exon/CDS
Intron
AT3G61250.1

Sequence Viewer

Length: 900 bp
ATGGGAAGAACACCTTGTTGTGACAAGATTGGTTTGAAGAAAGGTCCTTGGACGCCTGAAGAAGATGAGGTTCTTGTTGCGCATATCAAGAAAAATGGACATGGAAGCTGGAGAACACTTCCTAAACTTGCTGGTTTACTTCGCTGTGGGAAGAGTTGCAGGCTGAGATGGACAAACTATCTGAGACCAGACATAAAGAGAGGTCCTTTCACTGCTGATGAAGAGAAACTTGTTATCCAGCTTCATGCCATTCTCGGCAACAGGTGGGCTGCTATTGCAGCACAGCTTCCAGGAAGAACAGACAACGAGATCAAGAACTTATGGAACACTCATTTGAAGAAACGTCTTTTATCTATGGGTCTTGATCCCAGAACTCATGAGCCATTACCTTCATATGGGTTAGCTAAACAAGCTCCATCTTCACCAACAACTCGCCACATGGCTCAATGGGAAAGTGCTAGGGTTGAAGCTGAGGCAAGGCTTTCTAGAGAATCAATGCTCTTTAGCCCTTCTTTTTACTCTGGTGTAGTAAAAACTGAATGTGATCACTTCTTACGCATTTGGAATTCCGAGATTGGTGAAGCTTTCAGGAATCTCGCTCCATTAGATGAATCAACTATTACTAGTCAAAGCCCTTGCTCGAGGGCAACATCGACCTCATCTGCACTTCTGAAGAGCTCAACAAATTCTTGGGGAGGGAAAGAGGTTACTGTGGCGATTCATGGCTCAGATTATTCTCCATATTCAAATGATCTTGAAGATGATTCAACAGACTCTGCTCTTCAACTTCTGCTTGATTTCCCTATAAGCGATGATGATATGAGCTTCTTGGAAGAGAACATTGACAGCTACTCACAGGCTCCTCCTATTGGTCTTGTTTCCATGGTTTCCAAATTCTAG

Protein Analysis

299

Amino Acids

33.29

Weight (kDa)

6.46

Isoelectric Point (pI)

55.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 14 - 61 5.4e-15 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 17 - 76 3.2e-11 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 67 - 112 1.6e-16 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 70 - 114 3.4e-06 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 81
AclWI GGATC 1 cut(s) 359
AcsI RAATTY 3 cut(s) 565, 685, 893
AcuI CTGAAG 2 cut(s) 78, 692
AcyI GRCGYC 1 cut(s) 53
AfiI CCNNNNNNNGG 2 cut(s) 395, 869
AgsI TTSAA 7 cut(s) 37, 337, 467, 747, 758, 768, 785
AhlI ACTAGT 1 cut(s) 623
AjnI CCWGG 1 cut(s) 289
Alw21I GWGCWC 1 cut(s) 680
Alw26I GTCTC 1 cut(s) 178
AlwI GGATC 1 cut(s) 359
AlwNI CAGNNNCTG 1 cut(s) 776
Ama87I CYCGRG 1 cut(s) 640
ApeKI GCWGC 2 cut(s) 269, 278
ApoI RAATTY 3 cut(s) 565, 685, 893
AspLEI GCGC 1 cut(s) 82
AspS9I GGNCC 2 cut(s) 44, 203
AsuHPI GGTGA 2 cut(s) 414, 590
AvaI CYCGRG 1 cut(s) 640
AvaII GGWCC 2 cut(s) 44, 203
BanII GRGCYC 1 cut(s) 680
Bbv12I GWGCWC 1 cut(s) 680
BbvCI CCTCAGC 1 cut(s) 471
BbvI GCAGC 2 cut(s) 256, 290
BccI CCATC 2 cut(s) 162, 424
BciT130I CCWGG 1 cut(s) 291
BclI TGATCA 1 cut(s) 544
BcoDI GTCTC 1 cut(s) 178
BcuI ACTAGT 1 cut(s) 623
BfaI CTAG 4 cut(s) 459, 486, 624, 898
BisI GCNGC 2 cut(s) 270, 279
BlsI GCNGC 2 cut(s) 271, 280
Bme1390I CCNGG 1 cut(s) 291
Bme18I GGWCC 2 cut(s) 44, 203
BmeT110I CYCGRG 1 cut(s) 640
BmgT120I GGNCC 2 cut(s) 44, 203
BmiI GGNNCC 1 cut(s) 861
BmrFI CCNGG 1 cut(s) 291
BpmI CTGGAG 1 cut(s) 130
Bpu10I CCTNAGC 1 cut(s) 471
BsaHI GRCGYC 1 cut(s) 53
BsaI GGTCTC 1 cut(s) 178
BsaJI CCNNGG 2 cut(s) 47, 882
Bsc4I CCNNNNNNNGG 2 cut(s) 395, 869
BseBI CCWGG 1 cut(s) 291
BseDI CCNNGG 2 cut(s) 47, 882
BseLI CCNNNNNNNGG 2 cut(s) 395, 869
BseMII CTCAG 4 cut(s) 155, 173, 462, 741
BseRI GAGGAG 1 cut(s) 852
BseXI GCAGC 2 cut(s) 256, 290
BsgI GTGCAG 1 cut(s) 648
BsiHKAI GWGCWC 1 cut(s) 680
BsiHKCI CYCGRG 1 cut(s) 640
BslI CCNNNNNNNGG 2 cut(s) 395, 869
BsmAI GTCTC 1 cut(s) 178
Bso31I GGTCTC 1 cut(s) 178
BsoBI CYCGRG 1 cut(s) 640
Bsp1286I GDGCHC 1 cut(s) 680
Bsp143I GATC 4 cut(s) 309, 364, 544, 751
Bsp19I CCATGG 1 cut(s) 882
BspCNI CTCAG 4 cut(s) 156, 174, 463, 740
BspHI TCATGA 1 cut(s) 376
BspLI GGNNCC 1 cut(s) 861
BspPI GGATC 1 cut(s) 359
BspQI GCTCTTC 2 cut(s) 668, 786
BspTNI GGTCTC 1 cut(s) 178
BssECI CCNNGG 2 cut(s) 47, 882
BssMI GATC 4 cut(s) 309, 364, 544, 751
BssNI GRCGYC 1 cut(s) 53
BssT1I CCWWGG 2 cut(s) 47, 882
Bst2UI CCWGG 1 cut(s) 291
Bst4CI ACNGT 1 cut(s) 712
Bst6I CTCTTC 5 cut(s) 146, 216, 668, 786, 828
BstACI GRCGYC 1 cut(s) 53
BstC8I GCNNGC 1 cut(s) 161
BstDEI CTNAG 4 cut(s) 164, 182, 471, 727
BstDSI CCRYGG 1 cut(s) 882
BstHHI GCGC 1 cut(s) 82
BstKTI GATC 4 cut(s) 312, 367, 547, 754
BstMAI GTCTC 1 cut(s) 178
BstMBI GATC 4 cut(s) 309, 364, 544, 751
BstMWI GCNNNNNNNGC 3 cut(s) 275, 278, 410
BstNI CCWGG 1 cut(s) 291
BstSCI CCNGG 1 cut(s) 289
BstV1I GCAGC 2 cut(s) 256, 290
BtgI CCRYGG 1 cut(s) 882
BtgZI GCGATG 1 cut(s) 825
BtsI GCAGTG 1 cut(s) 210
BtsIMutI CAGTG 1 cut(s) 210
Cac8I GCNNGC 1 cut(s) 161
CaiI CAGNNNCTG 1 cut(s) 776
CciI TCATGA 1 cut(s) 376
CfoI GCGC 1 cut(s) 82
Cfr13I GGNCC 2 cut(s) 44, 203
CseI GACGC 1 cut(s) 61
CviAII CATG 6 cut(s) 101, 245, 377, 439, 722, 883
DdeI CTNAG 4 cut(s) 164, 182, 471, 727
DpnI GATC 4 cut(s) 311, 366, 546, 753
DpnII GATC 4 cut(s) 309, 364, 544, 751
Eam1104I CTCTTC 5 cut(s) 146, 216, 668, 786, 828
EarI CTCTTC 5 cut(s) 146, 216, 668, 786, 828
Ecl136II GAGCTC 1 cut(s) 678
Eco130I CCWWGG 2 cut(s) 47, 882
Eco24I GRGCYC 1 cut(s) 680
Eco31I GGTCTC 1 cut(s) 178
Eco47I GGWCC 2 cut(s) 44, 203
Eco53kI GAGCTC 1 cut(s) 678
Eco57I CTGAAG 2 cut(s) 78, 692
Eco88I CYCGRG 1 cut(s) 640
EcoICRI GAGCTC 1 cut(s) 678
EcoO109I RGGNCCY 2 cut(s) 44, 203
EcoRI GAATTC 1 cut(s) 565
EcoRII CCWGG 1 cut(s) 289
EcoT14I CCWWGG 2 cut(s) 47, 882
EcoT38I GRGCYC 1 cut(s) 680
ErhI CCWWGG 2 cut(s) 47, 882
FaeI CATG 6 cut(s) 104, 248, 380, 442, 725, 886
FalI AAGNNNNNCTT 3 cut(s) 30, 213, 245
FatI CATG 6 cut(s) 100, 244, 376, 438, 721, 882
FauNDI CATATG 1 cut(s) 394
FbaI TGATCA 1 cut(s) 544
Fnu4HI GCNGC 2 cut(s) 270, 279
FriOI GRGCYC 1 cut(s) 680
Fsp4HI GCNGC 2 cut(s) 270, 279
FspBI CTAG 4 cut(s) 459, 486, 624, 898
FspI TGCGCA 1 cut(s) 81
GlaI GCGC 1 cut(s) 81
GluI GCNGC 2 cut(s) 270, 279
GsuI CTGGAG 1 cut(s) 130
HgaI GACGC 1 cut(s) 61
HhaI GCGC 1 cut(s) 82
Hin1I GRCGYC 1 cut(s) 53
Hin1II CATG 6 cut(s) 104, 248, 380, 442, 725, 886
Hin6I GCGC 1 cut(s) 80
HinP1I GCGC 1 cut(s) 80
HindIII AAGCTT 1 cut(s) 582
HinfI GANTC 6 cut(s) 491, 592, 611, 718, 764, 773
HphI GGTGA 2 cut(s) 414, 590
Hpy166II GTNNAC 1 cut(s) 137
Hpy188I TCNGA 4 cut(s) 183, 571, 672, 730
Hpy188III TCNNGA 7 cut(s) 88, 313, 362, 377, 486, 589, 755
Hpy8I GTNNAC 1 cut(s) 137
HpyAV CCTTC 2 cut(s) 399, 519
HpyCH4III ACNGT 1 cut(s) 712
HpyCH4IV ACGT 1 cut(s) 343
HpyCH4V TGCA 3 cut(s) 159, 278, 665
HpyF10VI GCNNNNNNNGC 3 cut(s) 275, 278, 410
HpyF3I CTNAG 4 cut(s) 164, 182, 471, 727
HpySE526I ACGT 1 cut(s) 343
Hsp92I GRCGYC 1 cut(s) 53
Hsp92II CATG 6 cut(s) 104, 248, 380, 442, 725, 886
HspAI GCGC 1 cut(s) 80
Ksp22I TGATCA 1 cut(s) 544
Kzo9I GATC 4 cut(s) 309, 364, 544, 751
LguI GCTCTTC 2 cut(s) 668, 786
LmnI GCTCC 3 cut(s) 418, 604, 865
Lsp1109I GCAGC 2 cut(s) 256, 290
MaeI CTAG 4 cut(s) 459, 486, 624, 898
MaeII ACGT 1 cut(s) 343
MaeIII GTNAC 2 cut(s) 20, 706
MalI GATC 4 cut(s) 311, 366, 546, 753
MboI GATC 4 cut(s) 309, 364, 544, 751
MhlI GDGCHC 1 cut(s) 680
MluCI AATT 3 cut(s) 565, 685, 893
MlyI GAGTC 1 cut(s) 767
MnlI CCTC 8 cut(s) 61, 194, 466, 636, 667, 689, 697, 873
MspR9I CCNGG 1 cut(s) 291
MvaI CCWGG 1 cut(s) 291
MwoI GCNNNNNNNGC 3 cut(s) 275, 278, 410
NcoI CCATGG 1 cut(s) 882
NdeI CATATG 1 cut(s) 394
NdeII GATC 4 cut(s) 309, 364, 544, 751
NlaIII CATG 6 cut(s) 104, 248, 380, 442, 725, 886
NlaIV GGNNCC 1 cut(s) 861
NmeAIII GCCGAG 1 cut(s) 234
NmuCI GTSAC 1 cut(s) 20
NsbI TGCGCA 1 cut(s) 81
PaeR7I CTCGAG 1 cut(s) 640
PagI TCATGA 1 cut(s) 376
PciSI GCTCTTC 2 cut(s) 668, 786
PfeI GAWTC 5 cut(s) 491, 592, 611, 718, 764
PfoI TCCNGGA 1 cut(s) 289
PkrI GCNGC 2 cut(s) 271, 280
PleI GAGTC 1 cut(s) 767
PpsI GAGTC 1 cut(s) 767
PpuMI RGGWCCY 2 cut(s) 44, 203
Psp124BI GAGCTC 1 cut(s) 680
Psp5II RGGWCCY 2 cut(s) 44, 203
Psp6I CCWGG 1 cut(s) 289
PspGI CCWGG 1 cut(s) 289
PspN4I GGNNCC 1 cut(s) 861
PspPI GGNCC 2 cut(s) 44, 203
PspPPI RGGWCCY 2 cut(s) 44, 203
PspXI VCTCGAGB 1 cut(s) 640
PstNI CAGNNNCTG 1 cut(s) 776
SacI GAGCTC 1 cut(s) 680
SapI GCTCTTC 2 cut(s) 668, 786
SatI GCNGC 2 cut(s) 270, 279
Sau3AI GATC 4 cut(s) 309, 364, 544, 751
Sau96I GGNCC 2 cut(s) 44, 203
SchI GAGTC 1 cut(s) 767
ScrFI CCNGG 1 cut(s) 291
SduI GDGCHC 1 cut(s) 680
Sfr274I CTCGAG 1 cut(s) 640
SinI GGWCC 2 cut(s) 44, 203
SlaI CTCGAG 1 cut(s) 640
SmlI CTYRAG 1 cut(s) 640
SmoI CTYRAG 1 cut(s) 640
SpeI ACTAGT 1 cut(s) 623
Sse9I AATT 3 cut(s) 565, 685, 893
SspMI CTAG 4 cut(s) 459, 486, 624, 898
SstI GAGCTC 1 cut(s) 680
StyD4I CCNGG 1 cut(s) 289
StyI CCWWGG 2 cut(s) 47, 882
TaaI ACNGT 1 cut(s) 712
TaiI ACGT 1 cut(s) 346
TaqI TCGA 2 cut(s) 641, 653
TasI AATT 3 cut(s) 565, 685, 893
TfiI GAWTC 5 cut(s) 491, 592, 611, 718, 764
TscAI CASTG 1 cut(s) 217
TseFI GTSAC 1 cut(s) 20
TseI GCWGC 2 cut(s) 269, 278
Tsp45I GTSAC 1 cut(s) 20
TspDTI ATGAA 5 cut(s) 233, 234, 381, 624, 710
TspRI CASTG 1 cut(s) 217
VpaK11BI GGWCC 2 cut(s) 44, 203
XapI RAATTY 3 cut(s) 565, 685, 893
XbaI TCTAGA 1 cut(s) 485
XhoI CTCGAG 1 cut(s) 640
XspI CTAG 4 cut(s) 459, 486, 624, 898
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.