AT3G61460

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005870
Physical Location & Seq
Reverse (-)
22741511 .. 22742772
1262 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G61460.1

Sequence Viewer

Length: 513 bp
ATGGGCTTTCCCGTAGGTTACACAGAGGTTTTCTTACCGAAGCTTTTCGTACAAACGCTTTCGATTCTCGGATTCATCAGAACCATCGTCTTCTCTATCTTCCGCTTCTTGGGTCTCTCAGATTTTCTCGAAATGGATCAAACCTGGCCCGATTACACATCGTACCCGACCCGAATACCCGAAACCCGCTCACCTTTCTCCGCACTCCTAATTAGAGAGATCCTACCGGTTATCAAATTCGAAGAGTTAACGAATTCCGGCGAAGATCTACCGGAAAACTGCGCCGTTTGTCTATACGAATTCGAAGGAGAACAAGAGATCCGGTGGCTGAGAAATTGCAGACATATATTTCACCGGAGCTGTCTTGACCGTTGGATGGATCATGATCAGAAGACGTGTCCACTTTGTAGAACACCGTTTGTTCCAGATGAGATGCAAGAAGAGTTTAATCAACGGCTATGGGCTGCTTCTGGTGTTCATGATTTTCACTGTCCCGTGACCGAATTATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

170

Amino Acids

20.06

Weight (kDa)

4.9

Isoelectric Point (pI)

57.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 92 - 137 4e-07 RING-H2 zinc finger domain
zf-RING_2 PF13639 93 - 137 6.6e-13 Ring finger domain
zf-RING_11 PF17123 93 - 122 6.7e-09 RING-like zinc finger
zf-RING_5 PF14634 93 - 138 5.1e-08 zinc-RING finger domain
zf-C3HC4 PF00097 94 - 136 2.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 94 - 136 2.6e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 94 - 134 2.1e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 189
AciI CCGC 3 cut(s) 103, 187, 201
AclWI GGATC 4 cut(s) 144, 214, 313, 387
AcsI RAATTY 3 cut(s) 236, 253, 299
AfaI GTAC 2 cut(s) 51, 164
AfiI CCNNNNNNNGG 2 cut(s) 109, 376
AflIII ACRYGT 1 cut(s) 395
AgeI ACCGGT 1 cut(s) 226
AjiI CACGTC 1 cut(s) 396
AjnI CCWGG 1 cut(s) 143
AloI GAACNNNNNNTCC 2 cut(s) 303, 335
AluBI AGCT 2 cut(s) 43, 360
AluI AGCT 2 cut(s) 43, 360
Alw26I GTCTC 1 cut(s) 119
AlwI GGATC 4 cut(s) 144, 214, 313, 387
AoxI GGCC 1 cut(s) 146
ApeKI GCWGC 1 cut(s) 464
ApoI RAATTY 3 cut(s) 236, 253, 299
AsiGI ACCGGT 1 cut(s) 226
Asp700I GAANNNNTTC 1 cut(s) 44
AspLEI GCGC 1 cut(s) 284
AspS9I GGNCC 1 cut(s) 147
AsuHPI GGTGA 2 cut(s) 183, 344
AsuII TTCGAA 2 cut(s) 240, 303
BbsI GAAGAC 2 cut(s) 82, 398
BbvI GCAGC 1 cut(s) 451
BccI CCATC 2 cut(s) 92, 370
BceAI ACGGC 2 cut(s) 269, 470
BciT130I CCWGG 1 cut(s) 145
BclI TGATCA 1 cut(s) 385
BcoDI GTCTC 1 cut(s) 119
BglII AGATCT 1 cut(s) 265
BisI GCNGC 1 cut(s) 465
BlsI GCNGC 1 cut(s) 466
Bme1390I CCNGG 1 cut(s) 145
BmgBI CACGTC 1 cut(s) 396
BmgT120I GGNCC 1 cut(s) 147
BmrFI CCNGG 1 cut(s) 145
BmsI GCATC 1 cut(s) 423
BpiI GAAGAC 2 cut(s) 82, 398
Bpu14I TTCGAA 2 cut(s) 240, 303
BsaBI GATNNNNATC 1 cut(s) 384
BsaI GGTCTC 1 cut(s) 119
BsaWI WCCGGW 4 cut(s) 226, 271, 321, 354
Bsc4I CCNNNNNNNGG 2 cut(s) 109, 376
Bse118I RCCGGY 1 cut(s) 226
Bse8I GATNNNNATC 1 cut(s) 384
BseBI CCWGG 1 cut(s) 145
BseGI GGATG 1 cut(s) 381
BseJI GATNNNNATC 1 cut(s) 384
BseLI CCNNNNNNNGG 2 cut(s) 109, 376
BseMII CTCAG 2 cut(s) 132, 320
BseXI GCAGC 1 cut(s) 451
BshFI GGCC 1 cut(s) 148
BshTI ACCGGT 1 cut(s) 226
BsiSI CCGG 5 cut(s) 227, 258, 272, 322, 355
BslFI GGGAC 1 cut(s) 477
BslI CCNNNNNNNGG 2 cut(s) 109, 376
BsmAI GTCTC 1 cut(s) 119
BsmFI GGGAC 1 cut(s) 477
BsnI GGCC 1 cut(s) 148
Bso31I GGTCTC 1 cut(s) 119
Bsp119I TTCGAA 2 cut(s) 240, 303
Bsp143I GATC 6 cut(s) 136, 219, 265, 318, 379, 385
BspACI CCGC 3 cut(s) 103, 187, 201
BspANI GGCC 1 cut(s) 148
BspCNI CTCAG 2 cut(s) 131, 321
BspHI TCATGA 2 cut(s) 382, 478
BspPI GGATC 4 cut(s) 144, 214, 313, 387
BspT104I TTCGAA 2 cut(s) 240, 303
BspTNI GGTCTC 1 cut(s) 119
BsrBI CCGCTC 1 cut(s) 189
BsrFI RCCGGY 1 cut(s) 226
BssAI RCCGGY 1 cut(s) 226
BssMI GATC 6 cut(s) 136, 219, 265, 318, 379, 385
Bst2UI CCWGG 1 cut(s) 145
Bst4CI ACNGT 3 cut(s) 371, 417, 491
Bst6I CTCTTC 2 cut(s) 237, 435
BstBI TTCGAA 2 cut(s) 240, 303
BstDEI CTNAG 2 cut(s) 118, 329
BstF5I GGATG 1 cut(s) 381
BstHHI GCGC 1 cut(s) 284
BstKTI GATC 6 cut(s) 139, 222, 268, 321, 382, 388
BstMAI GTCTC 1 cut(s) 119
BstMBI GATC 6 cut(s) 136, 219, 265, 318, 379, 385
BstNI CCWGG 1 cut(s) 145
BstSCI CCNGG 1 cut(s) 143
BstV1I GCAGC 1 cut(s) 451
BstV2I GAAGAC 2 cut(s) 82, 398
BstX2I RGATCY 3 cut(s) 219, 265, 318
BstYI RGATCY 3 cut(s) 219, 265, 318
BsuRI GGCC 1 cut(s) 148
BtrI CACGTC 1 cut(s) 396
BtsCI GGATG 1 cut(s) 381
BtsIMutI CAGTG 1 cut(s) 487
CciI TCATGA 2 cut(s) 382, 478
CfoI GCGC 1 cut(s) 284
Cfr10I RCCGGY 1 cut(s) 226
Cfr13I GGNCC 1 cut(s) 147
Csp6I GTAC 2 cut(s) 50, 163
CspAI ACCGGT 1 cut(s) 226
CviAII CATG 2 cut(s) 383, 479
CviJI RGCY 7 cut(s) 6, 43, 148, 328, 360, 457, 464
CviKI_1 RGCY 7 cut(s) 6, 43, 148, 328, 360, 457, 464
CviQI GTAC 2 cut(s) 50, 163
DdeI CTNAG 2 cut(s) 118, 329
DpnI GATC 6 cut(s) 138, 221, 267, 320, 381, 387
DpnII GATC 6 cut(s) 136, 219, 265, 318, 379, 385
Eam1104I CTCTTC 2 cut(s) 237, 435
EarI CTCTTC 2 cut(s) 237, 435
Eco31I GGTCTC 1 cut(s) 119
EcoRI GAATTC 2 cut(s) 253, 299
EcoRII CCWGG 1 cut(s) 143
FaeI CATG 2 cut(s) 386, 482
FaiI YATR 7 cut(s) 295, 345, 347, 384, 460, 480, 511
FaqI GGGAC 1 cut(s) 477
FatI CATG 2 cut(s) 382, 478
FauI CCCGC 1 cut(s) 194
FbaI TGATCA 1 cut(s) 385
Fnu4HI GCNGC 1 cut(s) 465
FokI GGATG 1 cut(s) 388
Fsp4HI GCNGC 1 cut(s) 465
GlaI GCGC 1 cut(s) 283
GluI GCNGC 1 cut(s) 465
HaeIII GGCC 1 cut(s) 148
HapII CCGG 5 cut(s) 227, 258, 272, 322, 355
HhaI GCGC 1 cut(s) 284
Hin1II CATG 2 cut(s) 386, 482
Hin6I GCGC 1 cut(s) 282
HinP1I GCGC 1 cut(s) 282
HincII GTYRAC 1 cut(s) 249
HindII GTYRAC 1 cut(s) 249
HindIII AAGCTT 1 cut(s) 41
HinfI GANTC 2 cut(s) 64, 72
HpaI GTTAAC 1 cut(s) 249
HpaII CCGG 5 cut(s) 227, 258, 272, 322, 355
HphI GGTGA 2 cut(s) 183, 344
Hpy166II GTNNAC 2 cut(s) 249, 401
Hpy188I TCNGA 4 cut(s) 71, 80, 121, 390
Hpy188III TCNNGA 5 cut(s) 128, 365, 383, 425, 479
Hpy8I GTNNAC 2 cut(s) 249, 401
HpyAV CCTTC 1 cut(s) 299
HpyCH4III ACNGT 3 cut(s) 371, 417, 491
HpyCH4IV ACGT 1 cut(s) 395
HpyCH4V TGCA 2 cut(s) 339, 436
HpyF3I CTNAG 2 cut(s) 118, 329
HpySE526I ACGT 1 cut(s) 395
Hsp92II CATG 2 cut(s) 386, 482
HspAI GCGC 1 cut(s) 282
Ksp22I TGATCA 1 cut(s) 385
KspAI GTTAAC 1 cut(s) 249
Kzo9I GATC 6 cut(s) 136, 219, 265, 318, 379, 385
LmnI GCTCC 1 cut(s) 357
LpnPI CCDG 9 cut(s) 130, 157, 240, 271, 285, 335, 368, 438, 456
Lsp1109I GCAGC 1 cut(s) 451
LweI GCATC 1 cut(s) 423
MaeII ACGT 1 cut(s) 395
MaeIII GTNAC 2 cut(s) 17, 496
MalI GATC 6 cut(s) 138, 221, 267, 320, 381, 387
MbiI CCGCTC 1 cut(s) 189
MboI GATC 6 cut(s) 136, 219, 265, 318, 379, 385
MboII GAAGA 6 cut(s) 82, 91, 254, 275, 403, 452
MflI RGATCY 3 cut(s) 219, 265, 318
MluCI AATT 6 cut(s) 210, 236, 253, 299, 334, 503
MmeI TCCRAC 1 cut(s) 353
MnlI CCTC 1 cut(s) 19
MroXI GAANNNNTTC 1 cut(s) 44
MseI TTAA 2 cut(s) 248, 447
MspI CCGG 5 cut(s) 227, 258, 272, 322, 355
MspR9I CCNGG 1 cut(s) 145
MvaI CCWGG 1 cut(s) 145
NdeII GATC 6 cut(s) 136, 219, 265, 318, 379, 385
NlaIII CATG 2 cut(s) 386, 482
NmuCI GTSAC 1 cut(s) 496
NspV TTCGAA 2 cut(s) 240, 303
PagI TCATGA 2 cut(s) 382, 478
PdmI GAANNNNTTC 1 cut(s) 44
PfeI GAWTC 2 cut(s) 64, 72
PinAI ACCGGT 1 cut(s) 226
PkrI GCNGC 1 cut(s) 466
Psp6I CCWGG 1 cut(s) 143
PspGI CCWGG 1 cut(s) 143
PspPI GGNCC 1 cut(s) 147
PsuI RGATCY 3 cut(s) 219, 265, 318
RsaI GTAC 2 cut(s) 51, 164
RsaNI GTAC 2 cut(s) 50, 163
SaqAI TTAA 2 cut(s) 248, 447
SatI GCNGC 1 cut(s) 465
Sau3AI GATC 6 cut(s) 136, 219, 265, 318, 379, 385
Sau96I GGNCC 1 cut(s) 147
ScrFI CCNGG 1 cut(s) 145
SetI ASST 7 cut(s) 19, 30, 45, 146, 196, 362, 398
SfaNI GCATC 1 cut(s) 423
SfuI TTCGAA 2 cut(s) 240, 303
Sse9I AATT 6 cut(s) 210, 236, 253, 299, 334, 503
SsiI CCGC 3 cut(s) 103, 187, 201
StyD4I CCNGG 1 cut(s) 143
TaaI ACNGT 3 cut(s) 371, 417, 491
TaiI ACGT 1 cut(s) 398
TaqI TCGA 4 cut(s) 62, 129, 240, 303
TasI AATT 6 cut(s) 210, 236, 253, 299, 334, 503
TfiI GAWTC 2 cut(s) 64, 72
Tru1I TTAA 2 cut(s) 248, 447
Tru9I TTAA 2 cut(s) 248, 447
TscAI CASTG 1 cut(s) 494
TseFI GTSAC 1 cut(s) 496
TseI GCWGC 1 cut(s) 464
Tsp45I GTSAC 1 cut(s) 496
TspDTI ATGAA 2 cut(s) 64, 467
TspRI CASTG 1 cut(s) 494
XapI RAATTY 3 cut(s) 236, 253, 299
XmnI GAANNNNTTC 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.