AT3G63250

homocysteine S-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0010707
Physical Location & Seq
Reverse (-)
23369781 .. 23372823
3043 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G63250.1

Sequence Viewer

Length: 1002 bp
ATGACCGGAAACTCTTTTAACTCGATGAAAGATTTCCTGAAGCAGACAGGCGGCTATGCGGTAATAGACGGAGGGCTTGCAACGGAGTTCGAGAGACATGGAGCAGATCTCAACGATCCTCTCTGGAGCGCCAAATGCCTTGTCACCTCTCCTCACCTCATTCACACCGTGCATCTCGATTACCTTGAAGCTGGTGCTGATATCATCTCCAGCGCTTCTTATCAGGCCACGATTCAGGGGTTTGAAGCAAAGGGATTTTCTAGAGAAGAGAGCGAATCTTTGCTTAAAAAGAGCGTAGAAATAGCGACTGAAGCTCGGAACTCGTATTATGACAAATGCGGAACTAGCTCTTCCATGGATGATAAGATTCTCAAAAAGCGCCCTATATTAGTTGCAGCATCAGTTGGTAGCTACGGTGCATACTTGGCTGATGGGTCTGAATACAGTGGAATCTATGGTGATTCGATCACGCTGGAAAAGCTGAAGGATTTTCACCGCAGACGACTCCAAGTTCTGGCGGAATCTGGTGCTGATTTAATAGCATTTGAGACCATTCCAAACAAGATAGAGGCTCAGGCATTTGCTGATCTATTAGAAGAGGGAGATGTGAAGATTCCTGGGTGGTTTTCATTCAACTCAAAGGATGGCGTAAACGTGGTTAGCGGGGATTCCATCAAGGAGTGTATCTCCATAGCTGAAAATTGTGAGAAAGTAGTGGCGGTTGGAATCAACTGTACCCCTCCAAGATTCATAGAGGGGCTAGTTTTGGAGATAGAAAAGGTGACAAGCAAGCCCATACTAGTGTACCCAAACAGCGGAGAAAGCTATGATGCTGATCGGAAGGAGTGGGTGGAAAACACCGGAGTTGGGGATGAAGACTTTGTATCATACGTAGAGAAATGGATGGATGCAGGAGTGTCTCTCCTGGGAGGTTGCTGCCGCACAACACCAACCACCATCAGAGCCATCCACAAGAGACTCGTCAACCGCAGATCTCTTTAG

Protein Analysis

333

Amino Acids

36.45

Weight (kDa)

5.04

Isoelectric Point (pI)

37.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 21 - 327 9.9e-80 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010707)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63250 AT3G63250 AT3G63250
fragaria_vesca FvH4_2g23130 FvH4_2g23130
malus_domestica MD03G1260400.v1.1 MD11G1280700.v1.1
prunus_persica Prupe.8G236600_v2.0.a1
pyrus_communis pycom03g20810 pycom11g24850
rosa_chinensis RchiOBHm_Chr6g0290381
rosa_laevigata RLG00000012224
rosa_multiflora Rmu_sc0025482.1_g000001
rosa_roxburghii Rroxscaffold_7G00177120
rosa_rugosa Rorug06G0212100 Rorug06G0212100 Rorug06G0212200
rosa_samantha Rh6DG322700
rosa_wichuraiana Rw6G027840 Rw6G027940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 514
AclWI GGATC 1 cut(s) 110
AcuI CTGAAG 3 cut(s) 59, 330, 503
AdeI CACNNNGTG 1 cut(s) 169
AfaI GTAC 2 cut(s) 736, 806
AfeI AGCGCT 1 cut(s) 214
AfiI CCNNNNNNNGG 3 cut(s) 514, 815, 867
AgsI TTSAA 3 cut(s) 188, 245, 634
AhlI ACTAGT 1 cut(s) 799
AjnI CCWGG 2 cut(s) 616, 924
AluBI AGCT 7 cut(s) 191, 314, 348, 411, 481, 695, 825
AluI AGCT 7 cut(s) 191, 314, 348, 411, 481, 695, 825
Alw26I GTCTC 4 cut(s) 88, 542, 924, 970
AlwI GGATC 1 cut(s) 110
Aor51HI AGCGCT 1 cut(s) 214
AoxI GGCC 1 cut(s) 225
ApeKI GCWGC 2 cut(s) 395, 936
ArsI GACNNNNNNTTYG 2 cut(s) 126, 158
Asp700I GAANNNNTTC 1 cut(s) 32
AspLEI GCGC 3 cut(s) 131, 215, 381
AsuHPI GGTGA 5 cut(s) 136, 146, 470, 485, 793
BaeI ACNNNNGTAYC 2 cut(s) 788, 821
BbsI GAAGAC 1 cut(s) 882
BbvI GCAGC 2 cut(s) 407, 923
BccI CCATC 6 cut(s) 425, 638, 680, 898, 965, 974
BciT130I CCWGG 2 cut(s) 618, 926
BcoDI GTCTC 4 cut(s) 88, 542, 924, 970
BcuI ACTAGT 1 cut(s) 799
BfaI CTAG 4 cut(s) 261, 345, 761, 800
BfoI RGCGCY 3 cut(s) 132, 216, 382
BglII AGATCT 2 cut(s) 106, 992
BisI GCNGC 4 cut(s) 52, 396, 937, 940
BlsI GCNGC 4 cut(s) 53, 397, 938, 941
Bme1390I CCNGG 2 cut(s) 618, 926
BmrFI CCNGG 2 cut(s) 618, 926
BmsI GCATC 4 cut(s) 181, 407, 820, 898
BpiI GAAGAC 1 cut(s) 882
BplI GAGNNNNNCTC 6 cut(s) 93, 125, 671, 703, 906, 938
BpmI CTGGAG 2 cut(s) 145, 193
Bpu10I CCTNAGC 1 cut(s) 573
BsaAI YACGTR 1 cut(s) 892
BsaBI GATNNNNATC 1 cut(s) 834
BsaI GGTCTC 1 cut(s) 542
BsaJI CCNNGG 3 cut(s) 354, 617, 925
BsaWI WCCGGW 2 cut(s) 5, 860
Bsc4I CCNNNNNNNGG 3 cut(s) 514, 815, 867
Bse8I GATNNNNATC 1 cut(s) 834
BseBI CCWGG 2 cut(s) 618, 926
BseDI CCNNGG 3 cut(s) 354, 617, 925
BseGI GGATG 6 cut(s) 364, 649, 877, 909, 913, 966
BseJI GATNNNNATC 1 cut(s) 834
BseLI CCNNNNNNNGG 3 cut(s) 514, 815, 867
BseMII CTCAG 1 cut(s) 587
BseRI GAGGAG 1 cut(s) 141
BseXI GCAGC 2 cut(s) 407, 923
BshFI GGCC 1 cut(s) 227
BsiSI CCGG 2 cut(s) 6, 861
BslI CCNNNNNNNGG 3 cut(s) 514, 815, 867
BsmAI GTCTC 4 cut(s) 88, 542, 924, 970
BsnI GGCC 1 cut(s) 227
Bso31I GGTCTC 1 cut(s) 542
Bsp143I GATC 6 cut(s) 106, 115, 465, 586, 835, 992
Bsp19I CCATGG 1 cut(s) 354
BspANI GGCC 1 cut(s) 227
BspCNI CTCAG 1 cut(s) 586
BspPI GGATC 1 cut(s) 110
BspQI GCTCTTC 1 cut(s) 355
BspTNI GGTCTC 1 cut(s) 542
BssECI CCNNGG 3 cut(s) 354, 617, 925
BssMI GATC 6 cut(s) 106, 115, 465, 586, 835, 992
BssT1I CCWWGG 1 cut(s) 354
Bst2UI CCWGG 2 cut(s) 618, 926
Bst4CI ACNGT 4 cut(s) 169, 416, 446, 734
Bst6I CTCTTC 3 cut(s) 261, 355, 591
BstBAI YACGTR 1 cut(s) 892
BstC8I GCNNGC 2 cut(s) 78, 791
BstDEI CTNAG 1 cut(s) 573
BstDSI CCRYGG 1 cut(s) 354
BstF5I GGATG 6 cut(s) 364, 649, 877, 909, 913, 966
BstH2I RGCGCY 3 cut(s) 132, 216, 382
BstHHI GCGC 3 cut(s) 131, 215, 381
BstKTI GATC 6 cut(s) 109, 118, 468, 589, 838, 995
BstMAI GTCTC 4 cut(s) 88, 542, 924, 970
BstMBI GATC 6 cut(s) 106, 115, 465, 586, 835, 992
BstMWI GCNNNNNNNGC 6 cut(s) 135, 311, 345, 425, 478, 822
BstNI CCWGG 2 cut(s) 618, 926
BstSCI CCNGG 2 cut(s) 616, 924
BstSNI TACGTA 1 cut(s) 892
BstV1I GCAGC 2 cut(s) 407, 923
BstV2I GAAGAC 1 cut(s) 882
BstX2I RGATCY 2 cut(s) 106, 992
BstYI RGATCY 2 cut(s) 106, 992
BsuRI GGCC 1 cut(s) 227
BtgI CCRYGG 1 cut(s) 354
BtsCI GGATG 6 cut(s) 364, 649, 877, 909, 913, 966
BtsIMutI CAGTG 1 cut(s) 451
Cac8I GCNNGC 2 cut(s) 78, 791
CfoI GCGC 3 cut(s) 131, 215, 381
Csp6I GTAC 2 cut(s) 735, 805
CviAII CATG 2 cut(s) 98, 355
CviQI GTAC 2 cut(s) 735, 805
DdeI CTNAG 1 cut(s) 573
DpnI GATC 6 cut(s) 108, 117, 467, 588, 837, 994
DpnII GATC 6 cut(s) 106, 115, 465, 586, 835, 992
DraIII CACNNNGTG 1 cut(s) 169
Eam1104I CTCTTC 3 cut(s) 261, 355, 591
EarI CTCTTC 3 cut(s) 261, 355, 591
EciI GGCGGA 1 cut(s) 533
Eco105I TACGTA 1 cut(s) 892
Eco130I CCWWGG 1 cut(s) 354
Eco31I GGTCTC 1 cut(s) 542
Eco32I GATATC 1 cut(s) 202
Eco47III AGCGCT 1 cut(s) 214
Eco57I CTGAAG 3 cut(s) 59, 330, 503
EcoRII CCWGG 2 cut(s) 616, 924
EcoRV GATATC 1 cut(s) 202
EcoT14I CCWWGG 1 cut(s) 354
ErhI CCWWGG 1 cut(s) 354
FaeI CATG 2 cut(s) 101, 358
FatI CATG 2 cut(s) 97, 354
FauI CCCGC 1 cut(s) 656
Fnu4HI GCNGC 4 cut(s) 52, 396, 937, 940
FokI GGATG 6 cut(s) 371, 656, 884, 916, 920, 953
Fsp4HI GCNGC 4 cut(s) 52, 396, 937, 940
FspBI CTAG 4 cut(s) 261, 345, 761, 800
GlaI GCGC 3 cut(s) 130, 214, 380
GluI GCNGC 4 cut(s) 52, 396, 937, 940
GsuI CTGGAG 2 cut(s) 145, 193
HaeII RGCGCY 3 cut(s) 132, 216, 382
HaeIII GGCC 1 cut(s) 227
HapII CCGG 2 cut(s) 6, 861
HhaI GCGC 3 cut(s) 131, 215, 381
Hin1II CATG 2 cut(s) 101, 358
Hin6I GCGC 3 cut(s) 129, 213, 379
HinP1I GCGC 3 cut(s) 129, 213, 379
HincII GTYRAC 1 cut(s) 985
HindII GTYRAC 1 cut(s) 985
HpaII CCGG 2 cut(s) 6, 861
HphI GGTGA 5 cut(s) 136, 146, 470, 485, 793
Hpy166II GTNNAC 3 cut(s) 652, 805, 985
Hpy188I TCNGA 4 cut(s) 318, 439, 840, 962
Hpy188III TCNNGA 5 cut(s) 37, 91, 124, 176, 261
Hpy8I GTNNAC 3 cut(s) 652, 805, 985
HpyAV CCTTC 2 cut(s) 478, 835
HpyCH4III ACNGT 4 cut(s) 169, 416, 446, 734
HpyCH4IV ACGT 2 cut(s) 654, 891
HpyCH4V TGCA 5 cut(s) 80, 172, 395, 419, 911
HpyF10VI GCNNNNNNNGC 6 cut(s) 135, 311, 345, 425, 478, 822
HpyF3I CTNAG 1 cut(s) 573
HpySE526I ACGT 2 cut(s) 654, 891
Hsp92II CATG 2 cut(s) 101, 358
HspAI GCGC 3 cut(s) 129, 213, 379
Kzo9I GATC 6 cut(s) 106, 115, 465, 586, 835, 992
LguI GCTCTTC 1 cut(s) 355
LmnI GCTCC 2 cut(s) 101, 126
Lsp1109I GCAGC 2 cut(s) 407, 923
LweI GCATC 4 cut(s) 181, 407, 820, 898
MaeI CTAG 4 cut(s) 261, 345, 761, 800
MaeII ACGT 2 cut(s) 654, 891
MaeIII GTNAC 2 cut(s) 142, 781
MalI GATC 6 cut(s) 108, 117, 467, 588, 837, 994
MboI GATC 6 cut(s) 106, 115, 465, 586, 835, 992
MboII GAAGA 5 cut(s) 278, 342, 608, 622, 887
MflI RGATCY 2 cut(s) 106, 992
MluCI AATT 1 cut(s) 700
MlyI GAGTC 2 cut(s) 498, 972
MmeI TCCRAC 1 cut(s) 703
MroXI GAANNNNTTC 1 cut(s) 32
MseI TTAA 3 cut(s) 18, 285, 536
MslI CAYNNNNRTG 1 cut(s) 800
MspA1I CMGCKG 1 cut(s) 816
MspI CCGG 2 cut(s) 6, 861
MspR9I CCNGG 2 cut(s) 618, 926
MvaI CCWGG 2 cut(s) 618, 926
MwoI GCNNNNNNNGC 6 cut(s) 135, 311, 345, 425, 478, 822
NcoI CCATGG 1 cut(s) 354
NdeII GATC 6 cut(s) 106, 115, 465, 586, 835, 992
NlaIII CATG 2 cut(s) 101, 358
NmuCI GTSAC 2 cut(s) 142, 781
PciSI GCTCTTC 1 cut(s) 355
PdmI GAANNNNTTC 1 cut(s) 32
PflMI CCANNNNNTGG 1 cut(s) 514
PkrI GCNGC 4 cut(s) 53, 397, 938, 941
PleI GAGTC 2 cut(s) 498, 972
PpsI GAGTC 2 cut(s) 498, 972
Ppu21I YACGTR 1 cut(s) 892
Psp6I CCWGG 2 cut(s) 616, 924
PspGI CCWGG 2 cut(s) 616, 924
PsuI RGATCY 2 cut(s) 106, 992
RsaI GTAC 2 cut(s) 736, 806
RsaNI GTAC 2 cut(s) 735, 805
RseI CAYNNNNRTG 1 cut(s) 800
SapI GCTCTTC 1 cut(s) 355
SaqAI TTAA 3 cut(s) 18, 285, 536
SatI GCNGC 4 cut(s) 52, 396, 937, 940
Sau3AI GATC 6 cut(s) 106, 115, 465, 586, 835, 992
SchI GAGTC 2 cut(s) 498, 972
ScrFI CCNGG 2 cut(s) 618, 926
SfaNI GCATC 4 cut(s) 181, 407, 820, 898
SmiMI CAYNNNNRTG 1 cut(s) 800
SnaBI TACGTA 1 cut(s) 892
SpeI ACTAGT 1 cut(s) 799
Sse9I AATT 1 cut(s) 700
SspMI CTAG 4 cut(s) 261, 345, 761, 800
StyD4I CCNGG 2 cut(s) 616, 924
StyI CCWWGG 1 cut(s) 354
TaaI ACNGT 4 cut(s) 169, 416, 446, 734
TaiI ACGT 2 cut(s) 657, 894
TaqI TCGA 4 cut(s) 23, 90, 177, 464
TasI AATT 1 cut(s) 700
TauI GCSGC 2 cut(s) 54, 942
Tru1I TTAA 3 cut(s) 18, 285, 536
Tru9I TTAA 3 cut(s) 18, 285, 536
TscAI CASTG 1 cut(s) 451
TseFI GTSAC 2 cut(s) 142, 781
TseI GCWGC 2 cut(s) 395, 936
Tsp45I GTSAC 2 cut(s) 142, 781
TspDTI ATGAA 4 cut(s) 41, 618, 739, 888
TspGWI ACGGA 2 cut(s) 84, 98
TspRI CASTG 1 cut(s) 451
Van91I CCANNNNNTGG 1 cut(s) 514
XbaI TCTAGA 1 cut(s) 260
XmnI GAANNNNTTC 1 cut(s) 32
XspI CTAG 4 cut(s) 261, 345, 761, 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.