FvH4_2g23130

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010707
Physical Location & Seq
Forward (+)
18976519 .. 18979111
2593 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g23130.t1

Sequence Viewer

Length: 1032 bp
ATGGGCAGTAGCAGCAAAACGGCGTCGTTGTCAATGAGGGAGTTTCTCCGGCAGGCCGGCGGCGTCGCCGTCATCGACGGTGGCCTGGCGACCGAGCTCGAGCGCCACGGCGCTGACCTCAACGATCCTCTCTGGAGCGCCAAGTGCCTCCTGACTTCGTCTCACCTCATTCGTCAGGTTCATCTTGATTATCTTGAAGCTGGTGCTGATATCATAATCACTGCTTCCTATCAGGCAACTATTCAGGGTTTCGAGGCAAAAGGGTTTACGACTGAAGAGAGTGAAACGTTGCTGAGGAAGAGTGTGGAGATTGCACGTGAGGCGCGGGATATCTATAACAAAAAATGTAGTGAGAAGTCATGTAATGACAGTGGGGATGTTAGGATTATGAAGCAGCGGCCGATTATAGTTGCAGCATCTGTGGGGAGCTATGGGGCTTATTTGGCTGATGGGTCTGAGTACAGTGGTGATTATGGTGAGGCTATGACGCTTAGAGCATTGAAGGAGTTTCATCGTAGAAGGGTCCAAGTCCTAACAGAAGCAGGTCCTGACCTACTTGCATTTGAAACCATTCCAAATAAGCTGGAAGCTCAGGCTTTTGCTGAGCTTTTGGACGAAGAAGACATAAAGCTTCCTGCGTGGTTTTGTTTCAACTCTAAAGATGGAATTAATGTTGTGAGTGGTGATTCTTTCCTTGAATGTGCCTTAGTTGCTGAATCCTGTGAGAAAGTTGTTGCTGTTGGGATCAACTGCACTCCTCCTAGATTTATTCATGGACTGATCGCAATAATTAAAAAGGTGGCTACAAAACCAATAGCTATATACCCAAATAGTGGCGAAAGTTATGATCCTGATAGAAAGGAGTGGGTACAAAACACTGGGGTATCAGATGAAGATTTTGTTTCATATGTAAACAAATGGTGTGAGGCAGGGGCTTTGCTTGTAGGTGGTTGTTGCAGAACAACTCCAAACACTATCAGGGCCATCTACAGAACCCTTCCCAACCGGTCATCATCTCCACCTGAACAGTAA

Protein Analysis

344

Amino Acids

37.53

Weight (kDa)

5.18

Isoelectric Point (pI)

35.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 24 - 332 4.5e-81 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010707)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63250 AT3G63250 AT3G63250
fragaria_vesca FvH4_2g23130 FvH4_2g23130
malus_domestica MD03G1260400.v1.1 MD11G1280700.v1.1
prunus_persica Prupe.8G236600_v2.0.a1
pyrus_communis pycom03g20810 pycom11g24850
rosa_chinensis RchiOBHm_Chr6g0290381
rosa_laevigata RLG00000012224
rosa_multiflora Rmu_sc0025482.1_g000001
rosa_roxburghii Rroxscaffold_7G00177120
rosa_rugosa Rorug06G0212100 Rorug06G0212100 Rorug06G0212200
rosa_samantha Rh6DG322700
rosa_wichuraiana Rw6G027840 Rw6G027940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 533
AccB7I CCANNNNNTGG 1 cut(s) 833
AccII CGCG 1 cut(s) 325
AciI CCGC 3 cut(s) 60, 325, 397
AclI AACGTT 1 cut(s) 287
AclWI GGATC 3 cut(s) 119, 752, 842
AcoI YGGCCR 1 cut(s) 398
AcuI CTGAAG 1 cut(s) 294
AcvI CACGTG 1 cut(s) 317
AcyI GRCGYC 2 cut(s) 23, 63
AfaI GTAC 2 cut(s) 461, 870
AfiI CCNNNNNNNGG 1 cut(s) 833
AgeI ACCGGT 1 cut(s) 1005
AgsI TTSAA 5 cut(s) 197, 502, 566, 652, 698
AjnI CCWGG 1 cut(s) 84
AluBI AGCT 8 cut(s) 97, 200, 429, 583, 590, 607, 631, 818
AluI AGCT 8 cut(s) 97, 200, 429, 583, 590, 607, 631, 818
Alw21I GWGCWC 1 cut(s) 99
Alw26I GTCTC 1 cut(s) 165
AlwI GGATC 3 cut(s) 119, 752, 842
AlwNI CAGNNNCTG 2 cut(s) 419, 548
Ama87I CYCGRG 1 cut(s) 98
AoxI GGCC 4 cut(s) 54, 82, 398, 981
ApeKI GCWGC 3 cut(s) 12, 394, 413
AseI ATTAAT 1 cut(s) 669
AsiGI ACCGGT 1 cut(s) 1005
Asp700I GAANNNNTTC 1 cut(s) 570
AspLEI GCGC 4 cut(s) 105, 113, 140, 325
AspS9I GGNCC 3 cut(s) 523, 545, 981
AsuHPI GGTGA 4 cut(s) 155, 479, 488, 695
AvaI CYCGRG 1 cut(s) 98
AvaII GGWCC 2 cut(s) 523, 545
BaeI ACNNNNGTAYC 2 cut(s) 867, 900
BanII GRGCYC 1 cut(s) 99
BbrPI CACGTG 1 cut(s) 317
BbsI GAAGAC 1 cut(s) 627
Bbv12I GWGCWC 1 cut(s) 99
BbvCI CCTCAGC 1 cut(s) 293
BbvI GCAGC 3 cut(s) 24, 406, 425
BccI CCATC 3 cut(s) 443, 656, 992
BceAI ACGGC 3 cut(s) 36, 53, 124
BciT130I CCWGG 1 cut(s) 86
BcoDI GTCTC 1 cut(s) 165
BfaI CTAG 1 cut(s) 762
BfmI CTRYAG 1 cut(s) 988
BfoI RGCGCY 3 cut(s) 106, 114, 141
BfuAI ACCTGC 1 cut(s) 533
BisI GCNGC 5 cut(s) 13, 61, 395, 398, 414
BlpI GCTNAGC 1 cut(s) 603
BlsI GCNGC 5 cut(s) 14, 62, 396, 399, 415
Bme1390I CCNGG 1 cut(s) 86
Bme18I GGWCC 2 cut(s) 523, 545
BmeT110I CYCGRG 1 cut(s) 98
BmgT120I GGNCC 3 cut(s) 523, 545, 981
BmiI GGNNCC 1 cut(s) 524
BmrFI CCNGG 1 cut(s) 86
BmrI ACTGGG 1 cut(s) 888
BmsI GCATC 1 cut(s) 425
BmuI ACTGGG 1 cut(s) 888
BpiI GAAGAC 1 cut(s) 627
BpmI CTGGAG 1 cut(s) 154
Bpu10I CCTNAGC 2 cut(s) 293, 591
Bpu1102I GCTNAGC 1 cut(s) 603
BsaAI YACGTR 1 cut(s) 317
BsaHI GRCGYC 2 cut(s) 23, 63
BsaJI CCNNGG 1 cut(s) 106
BsaWI WCCGGW 1 cut(s) 1005
Bsc4I CCNNNNNNNGG 1 cut(s) 833
Bse118I RCCGGY 2 cut(s) 56, 1005
Bse1I ACTGG 1 cut(s) 883
BseBI CCWGG 1 cut(s) 86
BseDI CCNNGG 1 cut(s) 106
BseGI GGATG 1 cut(s) 382
BseLI CCNNNNNNNGG 1 cut(s) 833
BseMII CTCAG 4 cut(s) 284, 447, 594, 605
BseNI ACTGG 1 cut(s) 883
BseRI GAGGAG 1 cut(s) 747
BseX3I CGGCCG 1 cut(s) 398
BseXI GCAGC 3 cut(s) 24, 406, 425
BsgI GTGCAG 1 cut(s) 736
Bsh1236I CGCG 1 cut(s) 325
Bsh1285I CGRYCG 2 cut(s) 93, 401
BshFI GGCC 4 cut(s) 56, 84, 400, 983
BshTI ACCGGT 1 cut(s) 1005
BsiEI CGRYCG 2 cut(s) 93, 401
BsiHKAI GWGCWC 1 cut(s) 99
BsiHKCI CYCGRG 1 cut(s) 98
BsiSI CCGG 3 cut(s) 49, 57, 1006
BslI CCNNNNNNNGG 1 cut(s) 833
BsmAI GTCTC 1 cut(s) 165
BsmBI CGTCTC 1 cut(s) 165
BsnI GGCC 4 cut(s) 56, 84, 400, 983
BsoBI CYCGRG 1 cut(s) 98
Bsp1286I GDGCHC 1 cut(s) 99
Bsp143I GATC 4 cut(s) 124, 744, 780, 847
Bsp1720I GCTNAGC 1 cut(s) 603
BspACI CCGC 3 cut(s) 60, 325, 397
BspANI GGCC 4 cut(s) 56, 84, 400, 983
BspCNI CTCAG 4 cut(s) 285, 448, 595, 604
BspFNI CGCG 1 cut(s) 325
BspLI GGNNCC 1 cut(s) 524
BspMI ACCTGC 1 cut(s) 533
BspPI GGATC 3 cut(s) 119, 752, 842
BsrFI RCCGGY 2 cut(s) 56, 1005
BsrI ACTGG 1 cut(s) 883
BssAI RCCGGY 2 cut(s) 56, 1005
BssECI CCNNGG 1 cut(s) 106
BssMI GATC 4 cut(s) 124, 744, 780, 847
BssNI GRCGYC 2 cut(s) 23, 63
Bst2UI CCWGG 1 cut(s) 86
Bst4CI ACNGT 4 cut(s) 80, 371, 464, 1029
Bst6I CTCTTC 2 cut(s) 270, 293
BstACI GRCGYC 2 cut(s) 23, 63
BstBAI YACGTR 1 cut(s) 317
BstC8I GCNNGC 2 cut(s) 54, 58
BstDEI CTNAG 6 cut(s) 293, 456, 491, 591, 603, 706
BstDSI CCRYGG 1 cut(s) 106
BstF5I GGATG 1 cut(s) 382
BstFNI CGCG 1 cut(s) 325
BstH2I RGCGCY 3 cut(s) 106, 114, 141
BstHHI GCGC 4 cut(s) 105, 113, 140, 325
BstKTI GATC 4 cut(s) 127, 747, 783, 850
BstMAI GTCTC 1 cut(s) 165
BstMBI GATC 4 cut(s) 124, 744, 780, 847
BstMCI CGRYCG 2 cut(s) 93, 401
BstMWI GCNNNNNNNGC 5 cut(s) 12, 144, 320, 443, 710
BstNI CCWGG 1 cut(s) 86
BstSCI CCNGG 1 cut(s) 84
BstSFI CTRYAG 1 cut(s) 988
BstUI CGCG 1 cut(s) 325
BstV1I GCAGC 3 cut(s) 24, 406, 425
BstV2I GAAGAC 1 cut(s) 627
BstZI CGGCCG 1 cut(s) 398
BsuRI GGCC 4 cut(s) 56, 84, 400, 983
BtgI CCRYGG 1 cut(s) 106
BtsCI GGATG 1 cut(s) 382
BtsI GCAGTG 1 cut(s) 219
BtsIMutI CAGTG 4 cut(s) 219, 376, 469, 876
BveI ACCTGC 1 cut(s) 533
Cac8I GCNNGC 2 cut(s) 54, 58
CaiI CAGNNNCTG 2 cut(s) 419, 548
CfoI GCGC 4 cut(s) 105, 113, 140, 325
Cfr10I RCCGGY 2 cut(s) 56, 1005
Cfr13I GGNCC 3 cut(s) 523, 545, 981
CseI GACGC 3 cut(s) 12, 52, 496
Csp6I GTAC 2 cut(s) 460, 869
CspAI ACCGGT 1 cut(s) 1005
CviAII CATG 2 cut(s) 360, 773
CviQI GTAC 2 cut(s) 460, 869
DdeI CTNAG 6 cut(s) 293, 456, 491, 591, 603, 706
DpnI GATC 4 cut(s) 126, 746, 782, 849
DpnII GATC 4 cut(s) 124, 744, 780, 847
EaeI YGGCCR 1 cut(s) 398
EagI CGGCCG 1 cut(s) 398
Eam1104I CTCTTC 2 cut(s) 270, 293
EarI CTCTTC 2 cut(s) 270, 293
Ecl136II GAGCTC 1 cut(s) 97
EclXI CGGCCG 1 cut(s) 398
Eco24I GRGCYC 1 cut(s) 99
Eco32I GATATC 2 cut(s) 211, 331
Eco47I GGWCC 2 cut(s) 523, 545
Eco52I CGGCCG 1 cut(s) 398
Eco53kI GAGCTC 1 cut(s) 97
Eco57I CTGAAG 1 cut(s) 294
Eco72I CACGTG 1 cut(s) 317
Eco88I CYCGRG 1 cut(s) 98
EcoICRI GAGCTC 1 cut(s) 97
EcoO109I RGGNCCY 1 cut(s) 545
EcoRII CCWGG 1 cut(s) 84
EcoRV GATATC 2 cut(s) 211, 331
EcoT38I GRGCYC 1 cut(s) 99
Esp3I CGTCTC 1 cut(s) 165
FaeI CATG 2 cut(s) 363, 776
FatI CATG 2 cut(s) 359, 772
FauI CCCGC 1 cut(s) 318
FauNDI CATATG 1 cut(s) 907
Fnu4HI GCNGC 5 cut(s) 13, 61, 395, 398, 414
FokI GGATG 1 cut(s) 389
FriOI GRGCYC 1 cut(s) 99
Fsp4HI GCNGC 5 cut(s) 13, 61, 395, 398, 414
FspBI CTAG 1 cut(s) 762
GlaI GCGC 4 cut(s) 104, 112, 139, 324
GluI GCNGC 5 cut(s) 13, 61, 395, 398, 414
GsuI CTGGAG 1 cut(s) 154
HaeII RGCGCY 3 cut(s) 106, 114, 141
HaeIII GGCC 4 cut(s) 56, 84, 400, 983
HapII CCGG 3 cut(s) 49, 57, 1006
HgaI GACGC 3 cut(s) 12, 52, 496
HhaI GCGC 4 cut(s) 105, 113, 140, 325
Hin1I GRCGYC 2 cut(s) 23, 63
Hin1II CATG 2 cut(s) 363, 776
Hin6I GCGC 4 cut(s) 103, 111, 138, 323
HinP1I GCGC 4 cut(s) 103, 111, 138, 323
HindIII AAGCTT 1 cut(s) 629
HinfI GANTC 2 cut(s) 686, 716
HpaII CCGG 3 cut(s) 49, 57, 1006
HphI GGTGA 4 cut(s) 155, 479, 488, 695
Hpy166II GTNNAC 2 cut(s) 267, 913
Hpy188I TCNGA 2 cut(s) 457, 889
Hpy188III TCNNGA 6 cut(s) 133, 151, 185, 194, 548, 851
Hpy8I GTNNAC 2 cut(s) 267, 913
Hpy99I CGWCG 3 cut(s) 28, 68, 80
HpyAV CCTTC 3 cut(s) 496, 513, 1007
HpyCH4III ACNGT 4 cut(s) 80, 371, 464, 1029
HpyCH4IV ACGT 2 cut(s) 287, 316
HpyCH4V TGCA 5 cut(s) 314, 413, 560, 753, 957
HpyF10VI GCNNNNNNNGC 5 cut(s) 12, 144, 320, 443, 710
HpyF3I CTNAG 6 cut(s) 293, 456, 491, 591, 603, 706
HpySE526I ACGT 2 cut(s) 287, 316
Hsp92I GRCGYC 2 cut(s) 23, 63
Hsp92II CATG 2 cut(s) 363, 776
HspAI GCGC 4 cut(s) 103, 111, 138, 323
KroI GCCGGC 1 cut(s) 56
KroNI GCCGGC 1 cut(s) 58
Kzo9I GATC 4 cut(s) 124, 744, 780, 847
LmnI GCTCC 2 cut(s) 135, 426
Lsp1109I GCAGC 3 cut(s) 24, 406, 425
LweI GCATC 1 cut(s) 425
MaeI CTAG 1 cut(s) 762
MaeII ACGT 2 cut(s) 287, 316
MalI GATC 4 cut(s) 126, 746, 782, 849
MboI GATC 4 cut(s) 124, 744, 780, 847
MboII GAAGA 5 cut(s) 287, 310, 629, 632, 905
MhlI GDGCHC 1 cut(s) 99
MluCI AATT 2 cut(s) 666, 789
MroNI GCCGGC 1 cut(s) 56
MroXI GAANNNNTTC 1 cut(s) 570
MseI TTAA 2 cut(s) 669, 792
MspA1I CMGCKG 1 cut(s) 397
MspI CCGG 3 cut(s) 49, 57, 1006
MspR9I CCNGG 1 cut(s) 86
MvaI CCWGG 1 cut(s) 86
MvnI CGCG 1 cut(s) 325
MwoI GCNNNNNNNGC 5 cut(s) 12, 144, 320, 443, 710
NaeI GCCGGC 1 cut(s) 58
NdeI CATATG 1 cut(s) 907
NdeII GATC 4 cut(s) 124, 744, 780, 847
NgoMIV GCCGGC 1 cut(s) 56
NlaIII CATG 2 cut(s) 363, 776
NlaIV GGNNCC 1 cut(s) 524
PaeR7I CTCGAG 1 cut(s) 98
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PdiI GCCGGC 1 cut(s) 58
PdmI GAANNNNTTC 1 cut(s) 570
PfeI GAWTC 2 cut(s) 686, 716
PflFI GACNNNGTC 1 cut(s) 157
PflMI CCANNNNNTGG 1 cut(s) 833
PinAI ACCGGT 1 cut(s) 1005
PkrI GCNGC 5 cut(s) 14, 62, 396, 399, 415
PmaCI CACGTG 1 cut(s) 317
PmlI CACGTG 1 cut(s) 317
Ppu21I YACGTR 1 cut(s) 317
PpuMI RGGWCCY 1 cut(s) 545
PshBI ATTAAT 1 cut(s) 669
Psp124BI GAGCTC 1 cut(s) 99
Psp1406I AACGTT 1 cut(s) 287
Psp5II RGGWCCY 1 cut(s) 545
Psp6I CCWGG 1 cut(s) 84
PspCI CACGTG 1 cut(s) 317
PspGI CCWGG 1 cut(s) 84
PspN4I GGNNCC 1 cut(s) 524
PspPI GGNCC 3 cut(s) 523, 545, 981
PspPPI RGGWCCY 1 cut(s) 545
PspXI VCTCGAGB 1 cut(s) 98
PstNI CAGNNNCTG 2 cut(s) 419, 548
PsyI GACNNNGTC 1 cut(s) 157
RsaI GTAC 2 cut(s) 461, 870
RsaNI GTAC 2 cut(s) 460, 869
SacI GAGCTC 1 cut(s) 99
SaqAI TTAA 2 cut(s) 669, 792
SatI GCNGC 5 cut(s) 13, 61, 395, 398, 414
Sau3AI GATC 4 cut(s) 124, 744, 780, 847
Sau96I GGNCC 3 cut(s) 523, 545, 981
ScrFI CCNGG 1 cut(s) 86
SduI GDGCHC 1 cut(s) 99
SfaNI GCATC 1 cut(s) 425
SfcI CTRYAG 1 cut(s) 988
Sfr274I CTCGAG 1 cut(s) 98
SinI GGWCC 2 cut(s) 523, 545
SlaI CTCGAG 1 cut(s) 98
SmlI CTYRAG 1 cut(s) 98
SmoI CTYRAG 1 cut(s) 98
Sse9I AATT 2 cut(s) 666, 789
SsiI CCGC 3 cut(s) 60, 325, 397
SspMI CTAG 1 cut(s) 762
SstI GAGCTC 1 cut(s) 99
StyD4I CCNGG 1 cut(s) 84
TaaI ACNGT 4 cut(s) 80, 371, 464, 1029
TaiI ACGT 2 cut(s) 290, 319
TaqI TCGA 3 cut(s) 75, 99, 252
TaqII GACCGA 1 cut(s) 107
TasI AATT 2 cut(s) 666, 789
TatI WGTACW 1 cut(s) 459
TauI GCSGC 2 cut(s) 63, 400
TfiI GAWTC 2 cut(s) 686, 716
Tru1I TTAA 2 cut(s) 669, 792
Tru9I TTAA 2 cut(s) 669, 792
TscAI CASTG 4 cut(s) 226, 376, 469, 883
TseI GCWGC 3 cut(s) 12, 394, 413
TspDTI ATGAA 6 cut(s) 170, 404, 500, 761, 894, 906
TspRI CASTG 4 cut(s) 226, 376, 469, 883
Tth111I GACNNNGTC 1 cut(s) 157
Van91I CCANNNNNTGG 1 cut(s) 833
VpaK11BI GGWCC 2 cut(s) 523, 545
VspI ATTAAT 1 cut(s) 669
XhoI CTCGAG 1 cut(s) 98
XmnI GAANNNNTTC 1 cut(s) 570
XspI CTAG 1 cut(s) 762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.