Rw6G027840

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0010707
Physical Location & Seq
Forward (+)
51833144 .. 51835429
2286 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G027840.1

Sequence Viewer

Length: 1047 bp
ATGGGCAGCCGCAGCAGCAGCAGCAGCAGCGTCAAAATGGCGTCGTCGTCAATGAGGGAGTTTCTCCGGCAGGCAGGCGGCGTCGCCGTCGTCGACGGTGGTCTGGCGACCGAGCTCGAGCGGCACGGCGCTGACCTCAACGATCCTCTCTGGAGCGCCAAGTGCCTCCTCACTTCCTCTCACCTCATTCGCGCCGTTCATCTTGATTATCTTGAAGCTGGTGCTGATATCATAATCACAGCTTCTTATCAGGCAACTATTCAAGGTTTTGAGGCCAAAGGGTTTTCGACTGAGGAGAGTGAATCATTGCTTAGGAAGAGTGTGGAGATTGCGCGTGAGGCGCGAGATAGCTATTACGAAAGGTGTAGTGAGGGGTCTTGTAATGATAGTGATGGTAGAGTTCTCAAACGTCGGCCCATCATAGTTGCAGCATCTGTGGGGAGCTATGGGGCTTATTTGGCTGATGGGTCTGAGTACAGCGGTGATTATGGTGAGGCTATGACGCTGGGAGCATTAAAAGAGTTTCATCGTAGAAGAGTCCAAGTCCTAACTGAAGCAGGTCCTGACCTACTTGCATTTGAAACTATTCCAAATAAGCTGGAAGCCCAGGCTTTTGCTGAGCTTTTGGAAGAAGAAGACATAAAGCTTCCTGCATGGTTTTGTTTCAACTCTAAAGATGGAATCAATGTTGTTAGTGGTGATTCTTTCCTTGAATGTGCCTCGGTTGCTGAATCCTGTGAGAAAGTTGTTGCTGTGGGGATTAACTGTACTCCTCCTAGATTTATTCATGGATTGATCACAACAATTAAAAAGGTGGCTACAAAACCAATAGTTATATACCCAAATAGTGGCGAAAGTTATGATGCTGATCGAAAGGAGTGGGTACAAAATACTGGGGTTTCAGATGAAGATTTTGTTTCATATGTAAACAAATGGTGTGAGGCAGGGGCTTCCCTTGTGGGAGGTTGTTGCAGAACAACTCCAAATACTATCAGGGCCATCTACAAAACCCTTCCCAATCGGTCAACATCTCCACCTGAACAGTAA

Protein Analysis

348

Amino Acids

37.79

Weight (kDa)

5.06

Isoelectric Point (pI)

42.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 30 - 337 2e-82 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010707)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63250 AT3G63250 AT3G63250
fragaria_vesca FvH4_2g23130 FvH4_2g23130
malus_domestica MD03G1260400.v1.1 MD11G1280700.v1.1
prunus_persica Prupe.8G236600_v2.0.a1
pyrus_communis pycom03g20810 pycom11g24850
rosa_chinensis RchiOBHm_Chr6g0290381
rosa_laevigata RLG00000012224
rosa_multiflora Rmu_sc0025482.1_g000001
rosa_roxburghii Rroxscaffold_7G00177120
rosa_rugosa Rorug06G0212100 Rorug06G0212100 Rorug06G0212200
rosa_samantha Rh6DG322700
rosa_wichuraiana Rw6G027840 Rw6G027940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 548
AccB7I CCANNNNNTGG 1 cut(s) 848
AccBSI CCGCTC 1 cut(s) 121
AccI GTMKAC 1 cut(s) 93
AccII CGCG 3 cut(s) 192, 334, 343
AciI CCGC 4 cut(s) 10, 78, 121, 480
AclWI GGATC 1 cut(s) 137
AcuI CTGAAG 1 cut(s) 573
AcyI GRCGYC 2 cut(s) 41, 81
AfaI GTAC 3 cut(s) 476, 769, 885
AfiI CCNNNNNNNGG 1 cut(s) 848
AgsI TTSAA 5 cut(s) 215, 263, 581, 667, 713
AjnI CCWGG 1 cut(s) 606
AluBI AGCT 8 cut(s) 115, 218, 242, 351, 444, 598, 622, 646
AluI AGCT 8 cut(s) 115, 218, 242, 351, 444, 598, 622, 646
Alw21I GWGCWC 1 cut(s) 117
AlwI GGATC 1 cut(s) 137
AlwNI CAGNNNCTG 2 cut(s) 434, 563
Ama87I CYCGRG 1 cut(s) 116
AoxI GGCC 3 cut(s) 273, 413, 996
ApeKI GCWGC 8 cut(s) 6, 12, 15, 18, 21, 24, 27, 428
Asp700I GAANNNNTTC 1 cut(s) 585
AspLEI GCGC 5 cut(s) 131, 158, 194, 334, 343
AspS9I GGNCC 3 cut(s) 414, 560, 996
AsuHPI GGTGA 4 cut(s) 173, 494, 503, 710
AvaI CYCGRG 1 cut(s) 116
AvaII GGWCC 1 cut(s) 560
BanII GRGCYC 1 cut(s) 117
BbsI GAAGAC 1 cut(s) 642
Bbv12I GWGCWC 1 cut(s) 117
BbvI GCAGC 8 cut(s) 18, 24, 27, 30, 33, 36, 39, 440
BccI CCATC 5 cut(s) 386, 425, 458, 671, 1007
BceAI ACGGC 3 cut(s) 71, 142, 179
BciT130I CCWGG 1 cut(s) 608
BclI TGATCA 1 cut(s) 795
BfaI CTAG 1 cut(s) 777
BfoI RGCGCY 2 cut(s) 132, 159
BfuAI ACCTGC 1 cut(s) 548
BlpI GCTNAGC 1 cut(s) 618
Bme1390I CCNGG 1 cut(s) 608
Bme18I GGWCC 1 cut(s) 560
BmeT110I CYCGRG 1 cut(s) 116
BmgT120I GGNCC 3 cut(s) 414, 560, 996
BmrFI CCNGG 1 cut(s) 608
BmrI ACTGGG 1 cut(s) 903
BmsI GCATC 2 cut(s) 440, 853
BmuI ACTGGG 1 cut(s) 903
BoxI GACNNNNGTC 1 cut(s) 99
BpiI GAAGAC 1 cut(s) 642
BpmI CTGGAG 1 cut(s) 172
Bpu10I CCTNAGC 1 cut(s) 311
Bpu1102I GCTNAGC 1 cut(s) 618
BsaBI GATNNNNATC 1 cut(s) 867
BsaHI GRCGYC 2 cut(s) 41, 81
BsaJI CCNNGG 2 cut(s) 606, 720
Bsc4I CCNNNNNNNGG 1 cut(s) 848
Bse1I ACTGG 1 cut(s) 898
Bse3DI GCAATG 1 cut(s) 305
Bse8I GATNNNNATC 1 cut(s) 867
BseBI CCWGG 1 cut(s) 608
BseDI CCNNGG 2 cut(s) 606, 720
BseJI GATNNNNATC 1 cut(s) 867
BseLI CCNNNNNNNGG 1 cut(s) 848
BseMI GCAATG 1 cut(s) 305
BseMII CTCAG 3 cut(s) 282, 462, 609
BseNI ACTGG 1 cut(s) 898
BseRI GAGGAG 3 cut(s) 158, 308, 762
BseXI GCAGC 8 cut(s) 18, 24, 27, 30, 33, 36, 39, 440
BseYI CCCAGC 1 cut(s) 505
Bsh1236I CGCG 3 cut(s) 192, 334, 343
Bsh1285I CGRYCG 1 cut(s) 111
BshFI GGCC 3 cut(s) 275, 415, 998
BsiEI CGRYCG 1 cut(s) 111
BsiHKAI GWGCWC 1 cut(s) 117
BsiHKCI CYCGRG 1 cut(s) 116
BsiSI CCGG 1 cut(s) 67
BslI CCNNNNNNNGG 1 cut(s) 848
BsnI GGCC 3 cut(s) 275, 415, 998
BsoBI CYCGRG 1 cut(s) 116
Bsp1286I GDGCHC 1 cut(s) 117
Bsp143I GATC 3 cut(s) 142, 795, 868
Bsp1720I GCTNAGC 1 cut(s) 618
BspACI CCGC 4 cut(s) 10, 78, 121, 480
BspANI GGCC 3 cut(s) 275, 415, 998
BspCNI CTCAG 3 cut(s) 283, 463, 610
BspFNI CGCG 3 cut(s) 192, 334, 343
BspMI ACCTGC 1 cut(s) 548
BspPI GGATC 1 cut(s) 137
BsrBI CCGCTC 1 cut(s) 121
BsrDI GCAATG 1 cut(s) 305
BsrI ACTGG 1 cut(s) 898
BssECI CCNNGG 2 cut(s) 606, 720
BssMI GATC 3 cut(s) 142, 795, 868
BssNI GRCGYC 2 cut(s) 41, 81
Bst2UI CCWGG 1 cut(s) 608
Bst4CI ACNGT 3 cut(s) 98, 767, 1044
Bst6I CTCTTC 2 cut(s) 311, 529
BstACI GRCGYC 2 cut(s) 41, 81
BstC8I GCNNGC 2 cut(s) 72, 76
BstDEI CTNAG 4 cut(s) 291, 311, 471, 618
BstFNI CGCG 3 cut(s) 192, 334, 343
BstH2I RGCGCY 2 cut(s) 132, 159
BstHHI GCGC 5 cut(s) 131, 158, 194, 334, 343
BstKTI GATC 3 cut(s) 145, 798, 871
BstMBI GATC 3 cut(s) 142, 795, 868
BstMCI CGRYCG 1 cut(s) 111
BstNI CCWGG 1 cut(s) 608
BstPAI GACNNNNGTC 1 cut(s) 99
BstSCI CCNGG 1 cut(s) 606
BstUI CGCG 3 cut(s) 192, 334, 343
BstV1I GCAGC 8 cut(s) 18, 24, 27, 30, 33, 36, 39, 440
BstV2I GAAGAC 1 cut(s) 642
BsuRI GGCC 3 cut(s) 275, 415, 998
BveI ACCTGC 1 cut(s) 548
Cac8I GCNNGC 2 cut(s) 72, 76
CaiI CAGNNNCTG 2 cut(s) 434, 563
CfoI GCGC 5 cut(s) 131, 158, 194, 334, 343
Cfr13I GGNCC 3 cut(s) 414, 560, 996
CseI GACGC 4 cut(s) 19, 30, 70, 511
Csp6I GTAC 3 cut(s) 475, 768, 884
CviAII CATG 2 cut(s) 654, 788
CviQI GTAC 3 cut(s) 475, 768, 884
DdeI CTNAG 4 cut(s) 291, 311, 471, 618
DpnI GATC 3 cut(s) 144, 797, 870
DpnII GATC 3 cut(s) 142, 795, 868
Eam1104I CTCTTC 2 cut(s) 311, 529
EarI CTCTTC 2 cut(s) 311, 529
Ecl136II GAGCTC 1 cut(s) 115
Eco24I GRGCYC 1 cut(s) 117
Eco32I GATATC 1 cut(s) 229
Eco47I GGWCC 1 cut(s) 560
Eco53kI GAGCTC 1 cut(s) 115
Eco57I CTGAAG 1 cut(s) 573
Eco88I CYCGRG 1 cut(s) 116
EcoICRI GAGCTC 1 cut(s) 115
EcoO109I RGGNCCY 1 cut(s) 560
EcoRII CCWGG 1 cut(s) 606
EcoRV GATATC 1 cut(s) 229
EcoT38I GRGCYC 1 cut(s) 117
FaeI CATG 2 cut(s) 657, 791
FatI CATG 2 cut(s) 653, 787
FauNDI CATATG 1 cut(s) 922
FbaI TGATCA 1 cut(s) 795
FblI GTMKAC 1 cut(s) 93
FriOI GRGCYC 1 cut(s) 117
FspBI CTAG 1 cut(s) 777
GlaI GCGC 5 cut(s) 130, 157, 193, 333, 342
GsaI CCCAGC 1 cut(s) 509
GsuI CTGGAG 1 cut(s) 172
HaeII RGCGCY 2 cut(s) 132, 159
HaeIII GGCC 3 cut(s) 275, 415, 998
HapII CCGG 1 cut(s) 67
HgaI GACGC 4 cut(s) 19, 30, 70, 511
HhaI GCGC 5 cut(s) 131, 158, 194, 334, 343
Hin1I GRCGYC 2 cut(s) 41, 81
Hin1II CATG 2 cut(s) 657, 791
Hin6I GCGC 5 cut(s) 129, 156, 192, 332, 341
HinP1I GCGC 5 cut(s) 129, 156, 192, 332, 341
HincII GTYRAC 2 cut(s) 94, 1026
HindII GTYRAC 2 cut(s) 94, 1026
HindIII AAGCTT 1 cut(s) 644
HinfI GANTC 5 cut(s) 302, 537, 681, 701, 731
HpaII CCGG 1 cut(s) 67
HphI GGTGA 4 cut(s) 173, 494, 503, 710
Hpy166II GTNNAC 3 cut(s) 94, 928, 1026
Hpy188I TCNGA 2 cut(s) 472, 904
Hpy188III TCNNGA 4 cut(s) 151, 203, 212, 563
Hpy8I GTNNAC 3 cut(s) 94, 928, 1026
Hpy99I CGWCG 7 cut(s) 46, 49, 86, 92, 95, 98, 414
HpyAV CCTTC 1 cut(s) 1022
HpyCH4III ACNGT 3 cut(s) 98, 767, 1044
HpyCH4IV ACGT 1 cut(s) 409
HpyCH4V TGCA 4 cut(s) 428, 575, 653, 972
HpyF3I CTNAG 4 cut(s) 291, 311, 471, 618
HpySE526I ACGT 1 cut(s) 409
Hsp92I GRCGYC 2 cut(s) 41, 81
Hsp92II CATG 2 cut(s) 657, 791
HspAI GCGC 5 cut(s) 129, 156, 192, 332, 341
Ksp22I TGATCA 1 cut(s) 795
Kzo9I GATC 3 cut(s) 142, 795, 868
LmnI GCTCC 3 cut(s) 153, 441, 509
Lsp1109I GCAGC 8 cut(s) 18, 24, 27, 30, 33, 36, 39, 440
LweI GCATC 2 cut(s) 440, 853
MaeI CTAG 1 cut(s) 777
MaeII ACGT 1 cut(s) 409
MalI GATC 3 cut(s) 144, 797, 870
MbiI CCGCTC 1 cut(s) 121
MboI GATC 3 cut(s) 142, 795, 868
MboII GAAGA 6 cut(s) 328, 546, 641, 644, 647, 920
MhlI GDGCHC 1 cut(s) 117
MluCI AATT 1 cut(s) 804
MlyI GAGTC 1 cut(s) 546
MroXI GAANNNNTTC 1 cut(s) 585
MseI TTAA 3 cut(s) 515, 762, 807
MspA1I CMGCKG 1 cut(s) 480
MspI CCGG 1 cut(s) 67
MspR9I CCNGG 1 cut(s) 608
MvaI CCWGG 1 cut(s) 608
MvnI CGCG 3 cut(s) 192, 334, 343
NdeI CATATG 1 cut(s) 922
NdeII GATC 3 cut(s) 142, 795, 868
NlaIII CATG 2 cut(s) 657, 791
PaeR7I CTCGAG 1 cut(s) 116
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PdmI GAANNNNTTC 1 cut(s) 585
PfeI GAWTC 4 cut(s) 302, 681, 701, 731
PflMI CCANNNNNTGG 1 cut(s) 848
PleI GAGTC 1 cut(s) 545
PpsI GAGTC 1 cut(s) 545
PpuMI RGGWCCY 1 cut(s) 560
PshAI GACNNNNGTC 1 cut(s) 99
Psp124BI GAGCTC 1 cut(s) 117
Psp5II RGGWCCY 1 cut(s) 560
Psp6I CCWGG 1 cut(s) 606
PspFI CCCAGC 1 cut(s) 505
PspGI CCWGG 1 cut(s) 606
PspPI GGNCC 3 cut(s) 414, 560, 996
PspPPI RGGWCCY 1 cut(s) 560
PspXI VCTCGAGB 1 cut(s) 116
PstNI CAGNNNCTG 2 cut(s) 434, 563
RsaI GTAC 3 cut(s) 476, 769, 885
RsaNI GTAC 3 cut(s) 475, 768, 884
SacI GAGCTC 1 cut(s) 117
SalI GTCGAC 1 cut(s) 92
SaqAI TTAA 3 cut(s) 515, 762, 807
Sau3AI GATC 3 cut(s) 142, 795, 868
Sau96I GGNCC 3 cut(s) 414, 560, 996
SchI GAGTC 1 cut(s) 546
ScrFI CCNGG 1 cut(s) 608
SduI GDGCHC 1 cut(s) 117
SfaNI GCATC 2 cut(s) 440, 853
Sfr274I CTCGAG 1 cut(s) 116
SgrDI CGTCGACG 1 cut(s) 92
SinI GGWCC 1 cut(s) 560
SlaI CTCGAG 1 cut(s) 116
SmlI CTYRAG 1 cut(s) 116
SmoI CTYRAG 1 cut(s) 116
Sse9I AATT 1 cut(s) 804
SsiI CCGC 4 cut(s) 10, 78, 121, 480
SspMI CTAG 1 cut(s) 777
SstI GAGCTC 1 cut(s) 117
StyD4I CCNGG 1 cut(s) 606
TaaI ACNGT 3 cut(s) 98, 767, 1044
TaiI ACGT 1 cut(s) 412
TaqI TCGA 4 cut(s) 93, 117, 287, 871
TaqII GACCGA 2 cut(s) 125, 1011
TasI AATT 1 cut(s) 804
TatI WGTACW 2 cut(s) 474, 767
TauI GCSGC 3 cut(s) 12, 81, 124
TfiI GAWTC 4 cut(s) 302, 681, 701, 731
Tru1I TTAA 3 cut(s) 515, 762, 807
Tru9I TTAA 3 cut(s) 515, 762, 807
TseI GCWGC 8 cut(s) 6, 12, 15, 18, 21, 24, 27, 428
TspDTI ATGAA 5 cut(s) 188, 515, 776, 909, 921
Van91I CCANNNNNTGG 1 cut(s) 848
VpaK11BI GGWCC 1 cut(s) 560
XhoI CTCGAG 1 cut(s) 116
XmiI GTMKAC 1 cut(s) 93
XmnI GAANNNNTTC 1 cut(s) 585
XspI CTAG 1 cut(s) 777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.