RchiOBHm_Chr6g0290381

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0010707
Physical Location & Seq
Forward (+)
53418630 .. 53421434
2805 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26052

Sequence Viewer

Length: 1044 bp
ATGGCCAGTCGCAGCAGCAGCAGCGTCGAAACGGCGTCGTCGTCGTCAATGAGGGAGTTTCTCCGGCAGGCCGGCGGCGTCGCCGTCGTGGACGGTGGTCTGGCGACCGAGCTCGAGCGGCACGGCGCTGACCTCAACGATCCTCTCTGGAGCGCCAAGTGCCTCCTCACTTCCTCTCACCTCATTCGCGCCGTTCATCTTGATTATCTTGAAGCTGGTGCTGATATCATAATCACAGCTTCTTATCAGGCAACTATTCAAGGTTTTGAGGCCAAAGGGTTTTCGACTGAGGAGAGTGAATCATTGCTTAGGAAGAGTGTGGAGATTGCGCGTGAGGCGCGAGATATTTATTACGAAAGGTGTAGTGAGGGGTCTTGTAATGATAGTGATGGTAGAGTTCTCAAACGTCGGCCCATCATAGTTGCAGCATCTGTGGGGAGCTATGGGGCTTATTTGGCTGATGGGTCTGAGTACAGCGGTGATTATGGTGAGGCTATGACGCTGGGAGCATTAAAAGAGTTTCATCGTAGAAGAGTCCAAGTCCTAACTGAAGCAGGTCCTGACCTACTTGCATTTGAAACTATTCCAAATAAGCTGGAAGCCCAGGCTTTTGCTGAGCTTTTGGAAGAAGAAGACATAAAGCTTCCTGCATGGTTTTGTTTCAACTCTAAAGATGGAATCAATGTTGTTAGTGGTGATTCTTTCCTTGAATGTGCCTTGGTTGCTGAATCCTGTAAGAAAGTTGTTGCTGTGGGGATTAACTGTACTCCTCCTAGATTTATTCATGGACTGATCACAACAATTAAAAAGGTGGCTACAAAACCAATAGTTATATACCCAAATAGTGGCGAAAGTTATGATGCTGATCGAAAGGAGTGGGTACAAAATACTGGGGTTTCAGATGAAGATTTTGTTTCATATGTAAACAAATGGTGTGAGGCAGGGGCTTCCCTTGTGGGAGGTTGTTGCAGAACAACTCCAAATACTATCAGGGCCATCTACAAAACCCTTCCAAATCGGTCAACATCTCCACCTGAACAGTAA

Protein Analysis

347

Amino Acids

37.74

Weight (kDa)

5.06

Isoelectric Point (pI)

40.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 29 - 336 9.2e-82 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010707)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63250 AT3G63250 AT3G63250
fragaria_vesca FvH4_2g23130 FvH4_2g23130
malus_domestica MD03G1260400.v1.1 MD11G1280700.v1.1
prunus_persica Prupe.8G236600_v2.0.a1
pyrus_communis pycom03g20810 pycom11g24850
rosa_chinensis RchiOBHm_Chr6g0290381
rosa_laevigata RLG00000012224
rosa_multiflora Rmu_sc0025482.1_g000001
rosa_roxburghii Rroxscaffold_7G00177120
rosa_rugosa Rorug06G0212100 Rorug06G0212100 Rorug06G0212200
rosa_samantha Rh6DG322700
rosa_wichuraiana Rw6G027840 Rw6G027940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 545
AccB7I CCANNNNNTGG 1 cut(s) 845
AccBSI CCGCTC 1 cut(s) 118
AccII CGCG 3 cut(s) 189, 331, 340
AciI CCGC 3 cut(s) 75, 118, 477
AclWI GGATC 1 cut(s) 134
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 570
AcyI GRCGYC 2 cut(s) 35, 78
AfaI GTAC 3 cut(s) 473, 766, 882
AfiI CCNNNNNNNGG 1 cut(s) 845
AgsI TTSAA 5 cut(s) 212, 260, 578, 664, 710
AjnI CCWGG 1 cut(s) 603
AluBI AGCT 7 cut(s) 112, 215, 239, 441, 595, 619, 643
AluI AGCT 7 cut(s) 112, 215, 239, 441, 595, 619, 643
Alw21I GWGCWC 1 cut(s) 114
AlwI GGATC 1 cut(s) 134
AlwNI CAGNNNCTG 2 cut(s) 431, 560
Ama87I CYCGRG 1 cut(s) 113
AoxI GGCC 5 cut(s) 3, 69, 270, 410, 993
ApeKI GCWGC 5 cut(s) 12, 15, 18, 21, 425
Asp700I GAANNNNTTC 1 cut(s) 582
AspLEI GCGC 5 cut(s) 128, 155, 191, 331, 340
AspS9I GGNCC 3 cut(s) 411, 557, 993
AsuHPI GGTGA 4 cut(s) 170, 491, 500, 707
AvaI CYCGRG 1 cut(s) 113
AvaII GGWCC 1 cut(s) 557
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 114
BbsI GAAGAC 1 cut(s) 639
Bbv12I GWGCWC 1 cut(s) 114
BbvI GCAGC 5 cut(s) 24, 27, 30, 33, 437
BccI CCATC 5 cut(s) 383, 422, 455, 668, 1004
BceAI ACGGC 4 cut(s) 48, 68, 139, 176
BcgI CGANNNNNNTGC 2 cut(s) 7, 41
BciT130I CCWGG 1 cut(s) 605
BclI TGATCA 1 cut(s) 792
BfaI CTAG 1 cut(s) 774
BfoI RGCGCY 2 cut(s) 129, 156
BfuAI ACCTGC 1 cut(s) 545
BisI GCNGC 7 cut(s) 13, 16, 19, 22, 76, 119, 426
BlpI GCTNAGC 1 cut(s) 615
BlsI GCNGC 7 cut(s) 14, 17, 20, 23, 77, 120, 427
Bme1390I CCNGG 1 cut(s) 605
Bme18I GGWCC 1 cut(s) 557
BmeT110I CYCGRG 1 cut(s) 113
BmgT120I GGNCC 3 cut(s) 411, 557, 993
BmrFI CCNGG 1 cut(s) 605
BmrI ACTGGG 1 cut(s) 900
BmsI GCATC 2 cut(s) 437, 850
BmuI ACTGGG 1 cut(s) 900
BoxI GACNNNNGTC 1 cut(s) 96
BpiI GAAGAC 1 cut(s) 639
BpmI CTGGAG 1 cut(s) 169
Bpu10I CCTNAGC 1 cut(s) 308
Bpu1102I GCTNAGC 1 cut(s) 615
BsaBI GATNNNNATC 1 cut(s) 864
BsaHI GRCGYC 2 cut(s) 35, 78
BsaJI CCNNGG 2 cut(s) 603, 717
Bsc4I CCNNNNNNNGG 1 cut(s) 845
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 2 cut(s) 6, 895
Bse3DI GCAATG 1 cut(s) 302
Bse8I GATNNNNATC 1 cut(s) 864
BseBI CCWGG 1 cut(s) 605
BseDI CCNNGG 2 cut(s) 603, 717
BseJI GATNNNNATC 1 cut(s) 864
BseLI CCNNNNNNNGG 1 cut(s) 845
BseMI GCAATG 1 cut(s) 302
BseMII CTCAG 3 cut(s) 279, 459, 606
BseNI ACTGG 2 cut(s) 6, 895
BseRI GAGGAG 3 cut(s) 155, 305, 759
BseXI GCAGC 5 cut(s) 24, 27, 30, 33, 437
BseYI CCCAGC 1 cut(s) 502
Bsh1236I CGCG 3 cut(s) 189, 331, 340
Bsh1285I CGRYCG 1 cut(s) 108
BshFI GGCC 5 cut(s) 5, 71, 272, 412, 995
BsiEI CGRYCG 1 cut(s) 108
BsiHKAI GWGCWC 1 cut(s) 114
BsiHKCI CYCGRG 1 cut(s) 113
BsiSI CCGG 2 cut(s) 64, 72
BslI CCNNNNNNNGG 1 cut(s) 845
BsnI GGCC 5 cut(s) 5, 71, 272, 412, 995
BsoBI CYCGRG 1 cut(s) 113
Bsp1286I GDGCHC 1 cut(s) 114
Bsp143I GATC 3 cut(s) 139, 792, 865
Bsp1720I GCTNAGC 1 cut(s) 615
BspACI CCGC 3 cut(s) 75, 118, 477
BspANI GGCC 5 cut(s) 5, 71, 272, 412, 995
BspCNI CTCAG 3 cut(s) 280, 460, 607
BspFNI CGCG 3 cut(s) 189, 331, 340
BspMI ACCTGC 1 cut(s) 545
BspPI GGATC 1 cut(s) 134
BsrBI CCGCTC 1 cut(s) 118
BsrDI GCAATG 1 cut(s) 302
BsrFI RCCGGY 1 cut(s) 71
BsrI ACTGG 2 cut(s) 6, 895
BssAI RCCGGY 1 cut(s) 71
BssECI CCNNGG 2 cut(s) 603, 717
BssMI GATC 3 cut(s) 139, 792, 865
BssNI GRCGYC 2 cut(s) 35, 78
BssT1I CCWWGG 1 cut(s) 717
Bst2UI CCWGG 1 cut(s) 605
Bst4CI ACNGT 3 cut(s) 95, 764, 1041
Bst6I CTCTTC 2 cut(s) 308, 526
BstACI GRCGYC 2 cut(s) 35, 78
BstC8I GCNNGC 2 cut(s) 69, 73
BstDEI CTNAG 4 cut(s) 288, 308, 468, 615
BstFNI CGCG 3 cut(s) 189, 331, 340
BstH2I RGCGCY 2 cut(s) 129, 156
BstHHI GCGC 5 cut(s) 128, 155, 191, 331, 340
BstKTI GATC 3 cut(s) 142, 795, 868
BstMBI GATC 3 cut(s) 139, 792, 865
BstMCI CGRYCG 1 cut(s) 108
BstMWI GCNNNNNNNGC 8 cut(s) 18, 21, 118, 159, 335, 337, 455, 722
BstNI CCWGG 1 cut(s) 605
BstPAI GACNNNNGTC 1 cut(s) 96
BstSCI CCNGG 1 cut(s) 603
BstUI CGCG 3 cut(s) 189, 331, 340
BstV1I GCAGC 5 cut(s) 24, 27, 30, 33, 437
BstV2I GAAGAC 1 cut(s) 639
BsuRI GGCC 5 cut(s) 5, 71, 272, 412, 995
BveI ACCTGC 1 cut(s) 545
Cac8I GCNNGC 2 cut(s) 69, 73
CaiI CAGNNNCTG 2 cut(s) 431, 560
CfoI GCGC 5 cut(s) 128, 155, 191, 331, 340
Cfr10I RCCGGY 1 cut(s) 71
Cfr13I GGNCC 3 cut(s) 411, 557, 993
CseI GACGC 4 cut(s) 13, 24, 67, 508
Csp6I GTAC 3 cut(s) 472, 765, 881
CviAII CATG 2 cut(s) 651, 785
CviQI GTAC 3 cut(s) 472, 765, 881
DdeI CTNAG 4 cut(s) 288, 308, 468, 615
DpnI GATC 3 cut(s) 141, 794, 867
DpnII GATC 3 cut(s) 139, 792, 865
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 308, 526
EarI CTCTTC 2 cut(s) 308, 526
Ecl136II GAGCTC 1 cut(s) 112
Eco130I CCWWGG 1 cut(s) 717
Eco24I GRGCYC 1 cut(s) 114
Eco32I GATATC 1 cut(s) 226
Eco47I GGWCC 1 cut(s) 557
Eco53kI GAGCTC 1 cut(s) 112
Eco57I CTGAAG 1 cut(s) 570
Eco88I CYCGRG 1 cut(s) 113
EcoICRI GAGCTC 1 cut(s) 112
EcoO109I RGGNCCY 1 cut(s) 557
EcoRII CCWGG 1 cut(s) 603
EcoRV GATATC 1 cut(s) 226
EcoT14I CCWWGG 1 cut(s) 717
EcoT38I GRGCYC 1 cut(s) 114
ErhI CCWWGG 1 cut(s) 717
FaeI CATG 2 cut(s) 654, 788
FatI CATG 2 cut(s) 650, 784
FauNDI CATATG 1 cut(s) 919
FbaI TGATCA 1 cut(s) 792
Fnu4HI GCNGC 7 cut(s) 13, 16, 19, 22, 76, 119, 426
FriOI GRGCYC 1 cut(s) 114
Fsp4HI GCNGC 7 cut(s) 13, 16, 19, 22, 76, 119, 426
FspBI CTAG 1 cut(s) 774
GlaI GCGC 5 cut(s) 127, 154, 190, 330, 339
GluI GCNGC 7 cut(s) 13, 16, 19, 22, 76, 119, 426
GsaI CCCAGC 1 cut(s) 506
GsuI CTGGAG 1 cut(s) 169
HaeII RGCGCY 2 cut(s) 129, 156
HaeIII GGCC 5 cut(s) 5, 71, 272, 412, 995
HapII CCGG 2 cut(s) 64, 72
HgaI GACGC 4 cut(s) 13, 24, 67, 508
HhaI GCGC 5 cut(s) 128, 155, 191, 331, 340
Hin1I GRCGYC 2 cut(s) 35, 78
Hin1II CATG 2 cut(s) 654, 788
Hin6I GCGC 5 cut(s) 126, 153, 189, 329, 338
HinP1I GCGC 5 cut(s) 126, 153, 189, 329, 338
HincII GTYRAC 1 cut(s) 1023
HindII GTYRAC 1 cut(s) 1023
HindIII AAGCTT 1 cut(s) 641
HinfI GANTC 5 cut(s) 299, 534, 678, 698, 728
HpaII CCGG 2 cut(s) 64, 72
HphI GGTGA 4 cut(s) 170, 491, 500, 707
Hpy166II GTNNAC 3 cut(s) 91, 925, 1023
Hpy188I TCNGA 2 cut(s) 469, 901
Hpy188III TCNNGA 4 cut(s) 148, 200, 209, 560
Hpy8I GTNNAC 3 cut(s) 91, 925, 1023
Hpy99I CGWCG 7 cut(s) 29, 40, 43, 46, 83, 89, 411
HpyAV CCTTC 1 cut(s) 1019
HpyCH4III ACNGT 3 cut(s) 95, 764, 1041
HpyCH4IV ACGT 1 cut(s) 406
HpyCH4V TGCA 4 cut(s) 425, 572, 650, 969
HpyF10VI GCNNNNNNNGC 8 cut(s) 18, 21, 118, 159, 335, 337, 455, 722
HpyF3I CTNAG 4 cut(s) 288, 308, 468, 615
HpySE526I ACGT 1 cut(s) 406
Hsp92I GRCGYC 2 cut(s) 35, 78
Hsp92II CATG 2 cut(s) 654, 788
HspAI GCGC 5 cut(s) 126, 153, 189, 329, 338
KroI GCCGGC 1 cut(s) 71
KroNI GCCGGC 1 cut(s) 73
Ksp22I TGATCA 1 cut(s) 792
Kzo9I GATC 3 cut(s) 139, 792, 865
LmnI GCTCC 3 cut(s) 150, 438, 506
Lsp1109I GCAGC 5 cut(s) 24, 27, 30, 33, 437
LweI GCATC 2 cut(s) 437, 850
MaeI CTAG 1 cut(s) 774
MaeII ACGT 1 cut(s) 406
MalI GATC 3 cut(s) 141, 794, 867
MbiI CCGCTC 1 cut(s) 118
MboI GATC 3 cut(s) 139, 792, 865
MboII GAAGA 6 cut(s) 325, 543, 638, 641, 644, 917
MhlI GDGCHC 1 cut(s) 114
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 801
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 543
Mox20I TGGCCA 1 cut(s) 5
MroNI GCCGGC 1 cut(s) 71
MroXI GAANNNNTTC 1 cut(s) 582
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 512, 759, 804
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 477
MspI CCGG 2 cut(s) 64, 72
MspR9I CCNGG 1 cut(s) 605
MvaI CCWGG 1 cut(s) 605
MvnI CGCG 3 cut(s) 189, 331, 340
MwoI GCNNNNNNNGC 8 cut(s) 18, 21, 118, 159, 335, 337, 455, 722
NaeI GCCGGC 1 cut(s) 73
NdeI CATATG 1 cut(s) 919
NdeII GATC 3 cut(s) 139, 792, 865
NgoMIV GCCGGC 1 cut(s) 71
NlaIII CATG 2 cut(s) 654, 788
PaeR7I CTCGAG 1 cut(s) 113
PcsI WCGNNNNNNNCGW 1 cut(s) 38
PdiI GCCGGC 1 cut(s) 73
PdmI GAANNNNTTC 1 cut(s) 582
PfeI GAWTC 4 cut(s) 299, 678, 698, 728
PflMI CCANNNNNTGG 1 cut(s) 845
PkrI GCNGC 7 cut(s) 14, 17, 20, 23, 77, 120, 427
PleI GAGTC 1 cut(s) 542
PpsI GAGTC 1 cut(s) 542
PpuMI RGGWCCY 1 cut(s) 557
PshAI GACNNNNGTC 1 cut(s) 96
Psp124BI GAGCTC 1 cut(s) 114
Psp5II RGGWCCY 1 cut(s) 557
Psp6I CCWGG 1 cut(s) 603
PspFI CCCAGC 1 cut(s) 502
PspGI CCWGG 1 cut(s) 603
PspPI GGNCC 3 cut(s) 411, 557, 993
PspPPI RGGWCCY 1 cut(s) 557
PspXI VCTCGAGB 1 cut(s) 113
PstNI CAGNNNCTG 2 cut(s) 431, 560
RsaI GTAC 3 cut(s) 473, 766, 882
RsaNI GTAC 3 cut(s) 472, 765, 881
SacI GAGCTC 1 cut(s) 114
SaqAI TTAA 3 cut(s) 512, 759, 804
SatI GCNGC 7 cut(s) 13, 16, 19, 22, 76, 119, 426
Sau3AI GATC 3 cut(s) 139, 792, 865
Sau96I GGNCC 3 cut(s) 411, 557, 993
SchI GAGTC 1 cut(s) 543
ScrFI CCNGG 1 cut(s) 605
SduI GDGCHC 1 cut(s) 114
SfaNI GCATC 2 cut(s) 437, 850
Sfr274I CTCGAG 1 cut(s) 113
SinI GGWCC 1 cut(s) 557
SlaI CTCGAG 1 cut(s) 113
SmlI CTYRAG 1 cut(s) 113
SmoI CTYRAG 1 cut(s) 113
Sse9I AATT 1 cut(s) 801
SsiI CCGC 3 cut(s) 75, 118, 477
SspMI CTAG 1 cut(s) 774
SstI GAGCTC 1 cut(s) 114
StyD4I CCNGG 1 cut(s) 603
StyI CCWWGG 1 cut(s) 717
TaaI ACNGT 3 cut(s) 95, 764, 1041
TaiI ACGT 1 cut(s) 409
TaqI TCGA 4 cut(s) 27, 114, 284, 868
TaqII GACCGA 2 cut(s) 122, 1008
TasI AATT 1 cut(s) 801
TatI WGTACW 2 cut(s) 471, 764
TauI GCSGC 2 cut(s) 78, 121
TfiI GAWTC 4 cut(s) 299, 678, 698, 728
Tru1I TTAA 3 cut(s) 512, 759, 804
Tru9I TTAA 3 cut(s) 512, 759, 804
TseI GCWGC 5 cut(s) 12, 15, 18, 21, 425
TspDTI ATGAA 5 cut(s) 185, 512, 773, 906, 918
Van91I CCANNNNNTGG 1 cut(s) 845
VpaK11BI GGWCC 1 cut(s) 557
XhoI CTCGAG 1 cut(s) 113
XmnI GAANNNNTTC 1 cut(s) 582
XspI CTAG 1 cut(s) 774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.