MD03G1260400.v1.1

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0010707
Physical Location & Seq
Forward (+)
34725679 .. 34728890
3212 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1260400.v1.1.491

Sequence Viewer

Length: 1035 bp
ATGGCCAGCGGCAGCAGCGCCGAAACGTCGTCGTTAATGAGGGACTTACTCAGGCAGGCAGGCGGAGTCGCGGTGGTCGACGGTGGACTTTCCACCGAGCTCGAACGCCACGGCGCTGACCTCAACGATCCTCTCTGGAGCGCCAAATGCCTCCTCACTTCCCCTCACCTCATTCGCAAGGTACACCTTGATTACCTTGAAGCTGGTGCAGATATCATAATCACAGCATCGTATCAGGCCACCATTCAAGGTTTCAAGGCCAAAGGCTTTTCTACAGAAGAGAGTGAATCTTTGCTTAGGAAAAGTGTTGAAATTGCCCGAGAGGCACGAGATATCTATTACGACAGATGTACTCAATGCTCTTCTGCTGACAGTGGAAATGGTAGAATTCTCAAGCGTCGACCAATCTTAGTTGCGGCATCTGTGGGGAGCTATGGTGCTTATTTGGCTGATGGGTCTGAGTACAGTGGAAATTATGGTGAGGCTATGACGCTAGGAAGATTAAAAGATTTTCATCGGAGAAGGGTCCAAATCCTAGCAGAATCTGGTCCTGACCTACTTGCATTTGAAACAGTTCCAAATAAGCTGGAAGCTCAGGCTTATGCTGAGCTTTTGGAAGAAGAAAACATGCAACTTCCTGCATGGTTTTCTTTCAACTCTAAAGACGGCATCAATGTTGTTAGTGGTGATTCTCTCCTTGAATGCGCCACAGTTGCTGAATCATGCAAGAAAGTTGTTGCAGTTGGAATCAACTGCACTCCCCCTAGATTTATCCATGGACTGTTAACATTAATTACACAGGTGGCTACAAAACCAATTATTGTGTACCCAAATAGTGGTGAAAGCTATGATCCAGATAGAAAGATGTGGGTGCAAAACACTGGGGTTTCAGATGAAGATTTTGTCTCGTATGTAAACAAATGGTGTGAAGCAGGGGCTTCCCTTGTTGGAGGCTGTTGCAGAACAACACCAAATACTATCAGAGCAATATACACGACTCTTCCCAATAGGTCAACATCTCCACCTGAGCAGTAA

Protein Analysis

345

Amino Acids

37.4

Weight (kDa)

5.31

Isoelectric Point (pI)

39.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 25 - 332 2.9e-82 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010707)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63250 AT3G63250 AT3G63250
fragaria_vesca FvH4_2g23130 FvH4_2g23130
malus_domestica MD03G1260400.v1.1 MD11G1280700.v1.1
prunus_persica Prupe.8G236600_v2.0.a1
pyrus_communis pycom03g20810 pycom11g24850
rosa_chinensis RchiOBHm_Chr6g0290381
rosa_laevigata RLG00000012224
rosa_multiflora Rmu_sc0025482.1_g000001
rosa_roxburghii Rroxscaffold_7G00177120
rosa_rugosa Rorug06G0212100 Rorug06G0212100 Rorug06G0212200
rosa_samantha Rh6DG322700
rosa_wichuraiana Rw6G027840 Rw6G027940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 836
AccI GTMKAC 2 cut(s) 78, 400
AccII CGCG 1 cut(s) 71
AciI CCGC 4 cut(s) 9, 63, 71, 416
AclWI GGATC 2 cut(s) 122, 845
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 387
AfaI GTAC 4 cut(s) 183, 352, 464, 827
AfiI CCNNNNNNNGG 1 cut(s) 836
AgsI TTSAA 7 cut(s) 200, 248, 256, 311, 569, 655, 701
AluBI AGCT 7 cut(s) 100, 203, 432, 586, 593, 610, 846
AluI AGCT 7 cut(s) 100, 203, 432, 586, 593, 610, 846
Alw21I GWGCWC 1 cut(s) 102
Alw26I GTCTC 1 cut(s) 910
AlwI GGATC 2 cut(s) 122, 845
AlwNI CAGNNNCTG 2 cut(s) 545, 716
Ama87I CYCGRG 1 cut(s) 318
AoxI GGCC 3 cut(s) 3, 237, 258
ApeKI GCWGC 2 cut(s) 12, 15
ApoI RAATTY 1 cut(s) 387
AseI ATTAAT 1 cut(s) 791
Asp700I GAANNNNTTC 1 cut(s) 573
AspLEI GCGC 4 cut(s) 20, 116, 143, 707
AspS9I GGNCC 2 cut(s) 526, 548
AsuHPI GGTGA 4 cut(s) 158, 491, 698, 851
AvaI CYCGRG 1 cut(s) 318
AvaII GGWCC 2 cut(s) 526, 548
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 102
BauI CACGAG 1 cut(s) 327
Bbv12I GWGCWC 1 cut(s) 102
BbvI GCAGC 2 cut(s) 24, 27
BccI CCATC 1 cut(s) 446
BceAI ACGGC 2 cut(s) 127, 682
BcoDI GTCTC 1 cut(s) 910
BfaI CTAG 3 cut(s) 494, 536, 765
BfmI CTRYAG 1 cut(s) 273
BfoI RGCGCY 3 cut(s) 21, 117, 144
BglI GCCNNNNNGGC 1 cut(s) 323
BisI GCNGC 4 cut(s) 10, 13, 16, 417
BlpI GCTNAGC 1 cut(s) 606
BlsI GCNGC 4 cut(s) 11, 14, 17, 418
Bme18I GGWCC 2 cut(s) 526, 548
BmeT110I CYCGRG 1 cut(s) 318
BmgT120I GGNCC 2 cut(s) 526, 548
BmiI GGNNCC 1 cut(s) 527
BmrI ACTGGG 1 cut(s) 891
BmsI GCATC 3 cut(s) 236, 428, 678
BmuI ACTGGG 1 cut(s) 891
BpmI CTGGAG 1 cut(s) 157
Bpu10I CCTNAGC 3 cut(s) 296, 594, 1026
Bpu1102I GCTNAGC 1 cut(s) 606
BpuEI CTTGAG 1 cut(s) 377
BsaBI GATNNNNATC 1 cut(s) 513
BsaJI CCNNGG 2 cut(s) 109, 775
BsaXI ACNNNNNCTCC 4 cut(s) 57, 87, 421, 451
Bsc4I CCNNNNNNNGG 1 cut(s) 836
Bse1I ACTGG 1 cut(s) 886
Bse8I GATNNNNATC 1 cut(s) 513
BseDI CCNNGG 2 cut(s) 109, 775
BseJI GATNNNNATC 1 cut(s) 513
BseLI CCNNNNNNNGG 1 cut(s) 836
BseMII CTCAG 5 cut(s) 64, 450, 597, 608, 1017
BseNI ACTGG 1 cut(s) 886
BseRI GAGGAG 1 cut(s) 143
BseXI GCAGC 2 cut(s) 24, 27
BsgI GTGCAG 2 cut(s) 228, 739
Bsh1236I CGCG 1 cut(s) 71
BshFI GGCC 3 cut(s) 5, 239, 260
BsiHKAI GWGCWC 1 cut(s) 102
BsiHKCI CYCGRG 1 cut(s) 318
BslFI GGGAC 1 cut(s) 56
BslI CCNNNNNNNGG 1 cut(s) 836
BsmAI GTCTC 1 cut(s) 910
BsmFI GGGAC 1 cut(s) 56
BsmI GAATGC 1 cut(s) 707
BsnI GGCC 3 cut(s) 5, 239, 260
BsoBI CYCGRG 1 cut(s) 318
Bsp1286I GDGCHC 1 cut(s) 102
Bsp143I GATC 2 cut(s) 127, 850
Bsp1720I GCTNAGC 1 cut(s) 606
Bsp19I CCATGG 1 cut(s) 775
BspACI CCGC 4 cut(s) 9, 63, 71, 416
BspANI GGCC 3 cut(s) 5, 239, 260
BspCNI CTCAG 5 cut(s) 63, 451, 598, 607, 1018
BspFNI CGCG 1 cut(s) 71
BspLI GGNNCC 1 cut(s) 527
BspPI GGATC 2 cut(s) 122, 845
BspQI GCTCTTC 1 cut(s) 367
BsrI ACTGG 1 cut(s) 886
BssECI CCNNGG 2 cut(s) 109, 775
BssMI GATC 2 cut(s) 127, 850
BssSI CACGAG 1 cut(s) 327
BssT1I CCWWGG 1 cut(s) 775
Bst2BI CACGAG 1 cut(s) 327
Bst4CI ACNGT 6 cut(s) 83, 374, 467, 574, 712, 783
Bst6I CTCTTC 3 cut(s) 273, 367, 1005
BstC8I GCNNGC 3 cut(s) 7, 57, 61
BstDEI CTNAG 7 cut(s) 50, 296, 409, 459, 594, 606, 1026
BstDSI CCRYGG 2 cut(s) 109, 775
BstFNI CGCG 1 cut(s) 71
BstH2I RGCGCY 3 cut(s) 21, 117, 144
BstHHI GCGC 4 cut(s) 20, 116, 143, 707
BstKTI GATC 2 cut(s) 130, 853
BstMAI GTCTC 1 cut(s) 910
BstMBI GATC 2 cut(s) 127, 850
BstMWI GCNNNNNNNGC 5 cut(s) 15, 147, 323, 446, 713
BstNSI RCATGY 1 cut(s) 631
BstSFI CTRYAG 1 cut(s) 273
BstUI CGCG 1 cut(s) 71
BstV1I GCAGC 2 cut(s) 24, 27
BsuRI GGCC 3 cut(s) 5, 239, 260
BtgI CCRYGG 2 cut(s) 109, 775
BtsIMutI CAGTG 3 cut(s) 379, 472, 879
Cac8I GCNNGC 3 cut(s) 7, 57, 61
CaiI CAGNNNCTG 2 cut(s) 545, 716
CfoI GCGC 4 cut(s) 20, 116, 143, 707
Cfr13I GGNCC 2 cut(s) 526, 548
CseI GACGC 2 cut(s) 386, 499
Csp6I GTAC 4 cut(s) 182, 351, 463, 826
CviAII CATG 4 cut(s) 628, 642, 723, 776
CviQI GTAC 4 cut(s) 182, 351, 463, 826
DdeI CTNAG 7 cut(s) 50, 296, 409, 459, 594, 606, 1026
DpnI GATC 2 cut(s) 129, 852
DpnII GATC 2 cut(s) 127, 850
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 273, 367, 1005
EarI CTCTTC 3 cut(s) 273, 367, 1005
EciI GGCGGA 1 cut(s) 78
Ecl136II GAGCTC 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 775
Eco24I GRGCYC 1 cut(s) 102
Eco32I GATATC 2 cut(s) 214, 334
Eco47I GGWCC 2 cut(s) 526, 548
Eco53kI GAGCTC 1 cut(s) 100
Eco88I CYCGRG 1 cut(s) 318
EcoICRI GAGCTC 1 cut(s) 100
EcoRI GAATTC 1 cut(s) 387
EcoRV GATATC 2 cut(s) 214, 334
EcoT14I CCWWGG 1 cut(s) 775
EcoT38I GRGCYC 1 cut(s) 102
ErhI CCWWGG 1 cut(s) 775
FaeI CATG 4 cut(s) 631, 645, 726, 779
FaqI GGGAC 1 cut(s) 56
FatI CATG 4 cut(s) 627, 641, 722, 775
FblI GTMKAC 2 cut(s) 78, 400
Fnu4HI GCNGC 4 cut(s) 10, 13, 16, 417
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 4 cut(s) 10, 13, 16, 417
FspBI CTAG 3 cut(s) 494, 536, 765
GlaI GCGC 4 cut(s) 19, 115, 142, 706
GluI GCNGC 4 cut(s) 10, 13, 16, 417
GsuI CTGGAG 1 cut(s) 157
HaeII RGCGCY 3 cut(s) 21, 117, 144
HaeIII GGCC 3 cut(s) 5, 239, 260
HgaI GACGC 2 cut(s) 386, 499
HhaI GCGC 4 cut(s) 20, 116, 143, 707
Hin1II CATG 4 cut(s) 631, 645, 726, 779
Hin6I GCGC 4 cut(s) 18, 114, 141, 705
HinP1I GCGC 4 cut(s) 18, 114, 141, 705
HincII GTYRAC 4 cut(s) 79, 401, 786, 1014
HindII GTYRAC 4 cut(s) 79, 401, 786, 1014
HinfI GANTC 7 cut(s) 66, 287, 542, 689, 719, 747, 997
HpaI GTTAAC 1 cut(s) 786
HphI GGTGA 4 cut(s) 158, 491, 698, 851
Hpy166II GTNNAC 8 cut(s) 79, 86, 184, 401, 786, 826, 916, 1014
Hpy188I TCNGA 4 cut(s) 460, 519, 892, 983
Hpy188III TCNNGA 3 cut(s) 136, 551, 854
Hpy8I GTNNAC 8 cut(s) 79, 86, 184, 401, 786, 826, 916, 1014
Hpy99I CGWCG 4 cut(s) 31, 34, 83, 402
HpyAV CCTTC 1 cut(s) 516
HpyCH4III ACNGT 6 cut(s) 83, 374, 467, 574, 712, 783
HpyCH4IV ACGT 1 cut(s) 26
HpyCH4V TGCA 9 cut(s) 209, 563, 631, 641, 726, 740, 756, 874, 960
HpyF10VI GCNNNNNNNGC 5 cut(s) 15, 147, 323, 446, 713
HpyF3I CTNAG 7 cut(s) 50, 296, 409, 459, 594, 606, 1026
HpySE526I ACGT 1 cut(s) 26
Hsp92II CATG 4 cut(s) 631, 645, 726, 779
HspAI GCGC 4 cut(s) 18, 114, 141, 705
KspAI GTTAAC 1 cut(s) 786
Kzo9I GATC 2 cut(s) 127, 850
LguI GCTCTTC 1 cut(s) 367
LmnI GCTCC 2 cut(s) 138, 429
Lsp1109I GCAGC 2 cut(s) 24, 27
LweI GCATC 3 cut(s) 236, 428, 678
MaeI CTAG 3 cut(s) 494, 536, 765
MaeII ACGT 1 cut(s) 26
MalI GATC 2 cut(s) 129, 852
MboI GATC 2 cut(s) 127, 850
MboII GAAGA 7 cut(s) 290, 354, 510, 629, 632, 908, 992
MhlI GDGCHC 1 cut(s) 102
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 5 cut(s) 312, 387, 472, 792, 816
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 75, 991
MmeI TCCRAC 2 cut(s) 724, 928
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 573
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 35, 503, 785, 791
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 9
Mva1269I GAATGC 1 cut(s) 707
MvnI CGCG 1 cut(s) 71
MwoI GCNNNNNNNGC 5 cut(s) 15, 147, 323, 446, 713
NcoI CCATGG 1 cut(s) 775
NdeII GATC 2 cut(s) 127, 850
NlaIII CATG 4 cut(s) 631, 645, 726, 779
NlaIV GGNNCC 1 cut(s) 527
NspI RCATGY 1 cut(s) 631
PciSI GCTCTTC 1 cut(s) 367
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PctI GAATGC 1 cut(s) 707
PdmI GAANNNNTTC 1 cut(s) 573
PfeI GAWTC 5 cut(s) 287, 542, 689, 719, 747
PflMI CCANNNNNTGG 1 cut(s) 836
PkrI GCNGC 4 cut(s) 11, 14, 17, 418
PleI GAGTC 2 cut(s) 74, 991
PpsI GAGTC 2 cut(s) 74, 991
PshBI ATTAAT 1 cut(s) 791
Psp124BI GAGCTC 1 cut(s) 102
PspN4I GGNNCC 1 cut(s) 527
PspPI GGNCC 2 cut(s) 526, 548
PstNI CAGNNNCTG 2 cut(s) 545, 716
RsaI GTAC 4 cut(s) 183, 352, 464, 827
RsaNI GTAC 4 cut(s) 182, 351, 463, 826
SacI GAGCTC 1 cut(s) 102
SalI GTCGAC 2 cut(s) 77, 399
SapI GCTCTTC 1 cut(s) 367
SaqAI TTAA 4 cut(s) 35, 503, 785, 791
SatI GCNGC 4 cut(s) 10, 13, 16, 417
Sau3AI GATC 2 cut(s) 127, 850
Sau96I GGNCC 2 cut(s) 526, 548
SchI GAGTC 2 cut(s) 75, 991
SduI GDGCHC 1 cut(s) 102
SfaNI GCATC 3 cut(s) 236, 428, 678
SfcI CTRYAG 1 cut(s) 273
SinI GGWCC 2 cut(s) 526, 548
SmlI CTYRAG 1 cut(s) 392
SmoI CTYRAG 1 cut(s) 392
Sse9I AATT 5 cut(s) 312, 387, 472, 792, 816
SsiI CCGC 4 cut(s) 9, 63, 71, 416
SspMI CTAG 3 cut(s) 494, 536, 765
SstI GAGCTC 1 cut(s) 102
StyI CCWWGG 1 cut(s) 775
TaaI ACNGT 6 cut(s) 83, 374, 467, 574, 712, 783
TaiI ACGT 1 cut(s) 29
TaqI TCGA 3 cut(s) 78, 102, 400
TasI AATT 5 cut(s) 312, 387, 472, 792, 816
TatI WGTACW 2 cut(s) 350, 462
TauI GCSGC 2 cut(s) 12, 419
TfiI GAWTC 5 cut(s) 287, 542, 689, 719, 747
Tru1I TTAA 4 cut(s) 35, 503, 785, 791
Tru9I TTAA 4 cut(s) 35, 503, 785, 791
TscAI CASTG 3 cut(s) 379, 472, 886
TseI GCWGC 2 cut(s) 12, 15
TspDTI ATGAA 2 cut(s) 503, 909
TspRI CASTG 3 cut(s) 379, 472, 886
Van91I CCANNNNNTGG 1 cut(s) 836
VpaK11BI GGWCC 2 cut(s) 526, 548
VspI ATTAAT 1 cut(s) 791
XapI RAATTY 1 cut(s) 387
XceI RCATGY 1 cut(s) 631
XmiI GTMKAC 2 cut(s) 78, 400
XmnI GAANNNNTTC 1 cut(s) 573
XspI CTAG 3 cut(s) 494, 536, 765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.